FastQCFastQC Report
Thu 26 May 2016
SRR1547281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547281_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences442360
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.5696717605570124No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7360.16638032371823855No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6390.14445248214124243No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA4670.10557012388100188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG251.2834935E-680.02
TCGTTAG451.8189894E-1280.01
CGAACGG150.001309255580.02
CGAACCC150.001309255580.06
GATCGGA304.0543455E-880.09
GATCGCA204.0881147E-580.09
GTCGAAG204.0881147E-580.01
GCGAACG150.001309255580.01
CGACCGG150.001309255580.02
ATATGCG150.001309255580.01
TATTACG150.001309255580.01
GCGATAT150.001309255580.09
GACGATA304.0543455E-880.01
ATAGCGG1350.077.037032
GCGGGAT2500.075.25
TCGTAGG503.6379788E-1272.02
AAATGCG451.1641532E-1071.1111151
TGTTGCG403.7071004E-970.01
CGGGATC1200.070.06
TACGGGA1150.069.565214
Produced by FastQC (version 0.11.5)