##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547281_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	442360
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.037333845736505	33.0	31.0	34.0	31.0	34.0
2	32.4576611809386	34.0	31.0	34.0	31.0	34.0
3	32.58242155710281	34.0	31.0	34.0	31.0	34.0
4	36.095015372095126	37.0	35.0	37.0	35.0	37.0
5	36.051962202730806	37.0	35.0	37.0	35.0	37.0
6	36.112012840220636	37.0	36.0	37.0	35.0	37.0
7	36.33070576001447	37.0	37.0	37.0	35.0	37.0
8	36.32274844018446	37.0	37.0	37.0	35.0	37.0
9	38.118261144768965	39.0	39.0	39.0	37.0	39.0
10-11	37.66563093408084	39.0	37.5	39.0	35.0	39.0
12-13	36.70509200651054	39.0	35.0	39.0	33.0	39.0
14-15	37.79660796636224	40.0	35.0	41.0	33.0	41.0
16-17	37.98793855683154	40.0	35.0	41.0	34.0	41.0
18-19	37.905448051360885	40.0	36.0	41.0	34.0	41.0
20-21	37.72239804683968	40.0	35.0	41.0	34.0	41.0
22-23	37.50973076227507	39.0	35.0	41.0	33.0	41.0
24-25	37.47487792747988	39.0	35.0	41.0	34.0	41.0
26-27	37.45384754498599	39.0	35.0	41.0	34.0	41.0
28-29	37.407839768514336	39.0	35.0	41.0	33.0	41.0
30-31	37.28518288271995	39.0	35.0	41.0	33.0	41.0
32-33	36.99460394249028	39.0	35.0	41.0	33.0	41.0
34-35	36.770894746360426	39.0	35.0	41.0	32.5	41.0
36-37	36.55538023329415	39.0	35.0	41.0	31.5	41.0
38-39	36.486022696446334	38.0	35.0	41.0	31.0	41.0
40-41	36.36999954787956	38.0	35.0	41.0	31.0	41.0
42-43	36.34562912559906	38.0	35.0	41.0	31.0	41.0
44-45	36.19772018265666	38.0	35.0	41.0	31.0	41.0
46-47	36.067214485939054	38.0	35.0	40.5	31.0	41.0
48-49	35.98392711818428	38.0	35.0	40.0	31.0	41.0
50-51	35.757499547879554	37.0	35.0	40.0	30.5	41.0
52-53	35.542587485306086	37.0	35.0	40.0	30.0	41.0
54-55	35.374264173975945	36.0	35.0	40.0	29.5	41.0
56-57	35.113489013473185	36.0	34.5	40.0	29.0	41.0
58-59	34.84596821593273	36.0	34.0	39.0	28.0	41.0
60-61	34.45375146939145	35.0	34.0	39.0	27.5	41.0
62-63	33.974685776290805	35.0	33.5	39.0	26.5	40.0
64-65	33.601781354552855	35.0	33.0	38.0	26.0	40.0
66-67	33.252709331765985	35.0	33.0	37.0	25.0	40.0
68-69	32.8260263134099	35.0	33.0	36.5	23.5	39.0
70-71	32.504258974590826	35.0	33.0	36.0	22.5	39.0
72-73	32.23500768604757	35.0	33.0	36.0	21.0	38.5
74-75	31.955765665973416	35.0	32.5	35.0	20.0	37.5
76-77	30.057272357356	33.0	29.5	34.5	18.5	35.5
78-79	30.979903246224794	34.0	31.0	35.0	18.5	36.0
80-81	31.241515959851704	35.0	32.0	35.0	19.0	36.0
82-83	31.079612758838955	34.5	32.0	35.0	18.0	36.0
84-85	30.89274233655846	34.0	32.0	35.0	17.5	36.0
86	30.472079301926033	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	8.0
13	14.0
14	47.0
15	134.0
16	389.0
17	743.0
18	1564.0
19	2461.0
20	2976.0
21	3338.0
22	3062.0
23	2845.0
24	3147.0
25	3637.0
26	4240.0
27	5073.0
28	6359.0
29	7928.0
30	10344.0
31	12943.0
32	15727.0
33	20313.0
34	57392.0
35	52811.0
36	35943.0
37	63201.0
38	87374.0
39	38296.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.716249208789222	24.12243421647527	30.71932362781445	13.441992946921062
2	29.555565602676552	26.68437471742472	30.507279139162673	13.252780540736053
3	29.611854598064923	25.500497332489374	31.565240980197125	13.322407089248575
4	26.27995297947373	27.433312234379237	31.725291617686953	14.561443168460078
5	25.26968984537481	32.28682521023601	28.29550592277783	14.147979021611356
6	23.066506917442805	41.18093860204358	24.502667510624832	11.249886969888777
7	88.12392621394339	4.334026584682159	5.890225155981554	1.6518220453928927
8	88.59978298218645	3.5220182656659738	5.696717605570123	2.181481146577448
9	84.50944931729812	6.093905416402929	6.956325165023962	2.44032010127498
10-11	45.109413147662536	25.891468487204992	17.97303101546252	11.026087349669952
12-13	31.297472646713086	27.715548422099644	25.42126322452301	15.565715706664255
14-15	15.653653133194684	34.89895108056786	34.010082285920966	15.437313500316485
16-17	19.29853512975857	25.55000452120445	33.72762003797812	21.423840311058868
18-19	23.95334117008771	28.658445609910483	28.578985441721677	18.809227778280132
20-21	26.83142689212406	28.924518491726197	27.098177954607106	17.145876661542637
22-23	21.981643909937606	26.69895560177231	28.219323627814454	23.100076860475628
24-25	19.124129668143595	27.975404647798175	34.18980016276336	18.71066552129487
26-27	18.411700877113663	36.25542544533864	27.29959761280405	18.033276064743646
28-29	16.440455737408445	29.329731440455735	35.25748259336287	18.972330228772947
30-31	22.746970793019262	30.28155800705308	28.811262320282122	18.160208879645538
32-33	26.094357536847816	30.022040871688215	26.395469753142237	17.48813183832173
34-35	20.16931910661	29.163125056515053	29.368952889049645	21.2986029478253
36-37	23.875689483678453	29.675942671127586	28.569943032823947	17.878424812370014
38-39	20.641106790849083	32.431729812822134	26.64741387105525	20.279749525273534
40-41	20.561081472104167	28.643299575006782	27.36391174608916	23.431707206799892
42-43	22.327516050275793	27.442806763721855	26.727326159688943	23.50235102631341
44-45	19.589926756487927	33.831720770413234	25.90751876299846	20.67083371010037
46-47	21.75920065105344	31.10023510263134	26.494936251017272	20.645627995297946
48-49	22.303327606474365	26.365516773668507	30.655574645085448	20.67558097477168
50-51	19.32769689845375	31.489171715344966	27.325481508273803	21.85764987792748
52-53	22.977552219911384	27.340175422732614	29.32566235645176	20.35661000090424
54-55	22.859209693462336	27.400420472013742	30.237702323899086	19.50266751062483
56-57	23.296184103445157	25.07482593362872	29.280337281851885	22.34865268107424
58-59	23.88812279591283	27.972917985351298	27.749457455466136	20.389501763269735
60-61	24.75291617686952	30.57667962745275	25.474048286463514	19.196355909214212
62-63	23.307600144678542	26.92275522199114	29.955014015733795	19.814630617596528
64-65	19.81621303915363	32.29496337824396	29.727710462067098	18.161113120535312
66-67	19.06207613708292	29.398340717967265	25.536779998191516	26.002803146758296
68-69	18.602269644633328	33.865629803779726	23.675513156704948	23.856587394882
70-71	19.349624740030745	32.06166922868252	27.575391084184826	21.013314947101907
72-73	22.828465503210055	31.206144316846007	25.653766163305903	20.31162401663803
74-75	21.795483316755586	33.48630075051994	24.0191246948187	20.69909123790578
76-77	25.541979383307712	29.6336694095307	25.570462971335562	19.253888235826025
78-79	19.766931910661	29.931051632154805	29.3812731711728	20.920743286011394
80-81	16.888620128402206	29.253549145492357	30.374694818699698	23.48313590740573
82-83	18.99414504023872	28.042544533863822	30.608101998372366	22.355208427525092
84-85	17.653720951261416	28.09589474636043	28.759268469120176	25.491115833257982
86	19.261461253277872	27.629758567682433	30.450538023329415	22.65824215571028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	222.0
2	1024.5
3	1123.0
4	567.5
5	872.5
6	1298.5
7	1492.0
8	1546.0
9	1497.0
10	1227.0
11	969.5
12	825.0
13	781.5
14	792.0
15	803.0
16	836.5
17	909.5
18	937.0
19	965.5
20	1119.0
21	1221.0
22	1112.0
23	1149.0
24	1362.5
25	1723.5
26	2233.5
27	3051.5
28	4191.0
29	4729.0
30	6470.5
31	8052.5
32	8024.0
33	7546.5
34	8070.0
35	9690.0
36	10178.0
37	11347.5
38	13108.0
39	14728.5
40	16900.0
41	19439.5
42	22966.0
43	25095.0
44	25156.5
45	24980.0
46	25562.5
47	26655.5
48	25783.0
49	21601.0
50	18564.0
51	16434.0
52	13691.0
53	12215.0
54	11279.0
55	11338.0
56	10717.5
57	9965.0
58	9472.0
59	7771.0
60	5555.0
61	4056.5
62	3419.0
63	2736.0
64	2200.0
65	1716.5
66	1046.5
67	716.5
68	525.0
69	396.0
70	309.5
71	304.0
72	224.5
73	86.0
74	30.5
75	44.5
76	34.0
77	14.5
78	13.0
79	10.0
80	6.0
81	2.5
82	2.0
83	0.5
84	0.5
85	0.5
86	0.0
87	1.5
88	1.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	442360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.693593856553353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47199471725979	17.80811577227094
2	6.725999547205237	3.052742040072748
3	2.2400065284764445	1.5250139517981736
4	1.216999970286567	1.104724121964834
5	0.8299999446366658	0.9417840822273131
6	0.6459999565169733	0.8796036386728391
7	0.5049999660034312	0.8022184888238584
8	0.4279999711870126	0.7770286013387682
9	0.3599999757647763	0.7352723914537396
>10	6.403999568882298	36.8861649712626
>50	2.0539998617245847	30.900729994536558
>100	0.11499999225819243	3.702686524370128
>500	0.001999999865359868	0.31203689452126293
>1k	9.99999932679934E-4	0.571878526686242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2520	0.5696717605570124	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	736	0.16638032371823855	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	639	0.14445248214124243	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	467	0.10557012388100188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.260602224432589E-4	0.0	0.0	0.07211321095939958	0.0
2	2.260602224432589E-4	0.0	0.0	0.24504928112849264	0.0
3	2.260602224432589E-4	0.0	0.0	0.393796907496157	0.0
4	2.260602224432589E-4	0.0	0.0	0.6171444072700968	0.0
5	2.260602224432589E-4	0.0	0.0	1.112442354643277	0.0
6	2.260602224432589E-4	0.0	0.0	1.5175422732615969	0.0
7	2.260602224432589E-4	0.0	0.0	1.8003436115381137	0.0
8	2.260602224432589E-4	0.0	0.0	2.340175422732616	0.0
9	2.260602224432589E-4	0.0	0.0	2.4988696988877837	0.0
10	2.260602224432589E-4	0.0	0.0	2.7841576996111765	0.0
11	2.260602224432589E-4	0.0	0.0	3.2767429243150374	0.0
12	2.260602224432589E-4	0.0	0.0	3.6336920155529433	0.0
13	2.260602224432589E-4	0.0	0.0	3.761642101455828	0.0
14	2.260602224432589E-4	0.0	0.0	3.8041414232751607	0.0
15	2.260602224432589E-4	0.0	0.0	3.8782891762365495	0.0
16	2.260602224432589E-4	0.0	0.0	4.0430870783976856	0.0
17	4.521204448865178E-4	0.0	0.0	4.245863097929289	0.0
18	4.521204448865178E-4	0.0	0.0	4.503119631069717	0.0
19	4.521204448865178E-4	0.0	0.0	4.655710281218917	0.0
20	4.521204448865178E-4	0.0	0.0	4.8132742562618684	0.0
21	4.521204448865178E-4	0.0	0.0	4.990505470657383	0.0
22	4.521204448865178E-4	0.0	0.0	5.194411791301203	0.0
23	4.521204448865178E-4	0.0	0.0	5.4100732435120715	0.0
24	4.521204448865178E-4	0.0	0.0	5.56673297766525	0.0
25	4.521204448865178E-4	0.0	0.0	5.711185459806493	0.0
26	4.521204448865178E-4	0.0	0.0	5.826476173252554	0.0
27	4.521204448865178E-4	0.0	0.0	5.953295958043222	0.0
28	4.521204448865178E-4	0.0	0.0	6.090740573288724	0.0
29	4.521204448865178E-4	0.0	0.0	6.2349669952075235	0.0
30	4.521204448865178E-4	0.0	0.0	6.39773035536667	0.0
31	4.521204448865178E-4	0.0	0.0	6.563884618862465	0.0
32	4.521204448865178E-4	0.0	0.0	6.715118907677005	0.0
33	4.521204448865178E-4	0.0	0.0	6.866805316936432	0.0
34	4.521204448865178E-4	0.0	0.0	7.013970521746994	0.0
35	4.521204448865178E-4	0.0	0.0	7.206799891491094	0.0
36	4.521204448865178E-4	0.0	0.0	7.385387467221268	0.0
37	4.521204448865178E-4	0.0	0.0	7.561262320282123	0.0
38	4.521204448865178E-4	0.0	0.0	7.734198390451216	0.0
39	4.521204448865178E-4	0.0	0.0	7.923184736413781	0.0
40	4.521204448865178E-4	0.0	0.0	8.106067456370377	0.0
41	4.521204448865178E-4	0.0	0.0	8.29143683877385	0.0
42	4.521204448865178E-4	0.0	0.0	8.475449859842662	0.0
43	4.521204448865178E-4	0.0	0.0	8.635726557554932	0.0
44	4.521204448865178E-4	0.0	0.0	8.821548060403291	0.0
45	4.521204448865178E-4	0.0	0.0	8.997422913464147	0.0
46	4.521204448865178E-4	0.0	0.0	9.21331042589746	0.0
47	4.521204448865178E-4	0.0	0.0	9.435753684781625	0.0
48	4.521204448865178E-4	0.0	0.0	9.627678813635953	0.0
49	4.521204448865178E-4	0.0	0.0	9.830454833167556	0.0
50	4.521204448865178E-4	0.0	0.0	10.026222985803418	0.0
51	4.521204448865178E-4	0.0	0.0	10.223573559996384	0.0
52	4.521204448865178E-4	0.0	0.0	10.450764083551858	0.0
53	4.521204448865178E-4	0.0	0.0	10.652861922416133	0.0
54	4.521204448865178E-4	0.0	0.0	10.851342797721314	0.0
55	4.521204448865178E-4	0.0	0.0	11.05705760014468	0.0
56	4.521204448865178E-4	0.0	0.0	11.26322452301293	0.0
57	4.521204448865178E-4	0.0	0.0	11.486119902341985	0.0
58	4.521204448865178E-4	0.0	0.0	11.687313500316485	0.0
59	4.521204448865178E-4	0.0	0.0	11.89777556741116	0.0
60	4.521204448865178E-4	0.0	0.0	12.111854598064925	0.0
61	4.521204448865178E-4	0.0	0.0	12.332715435391988	0.0
62	4.521204448865178E-4	0.0	0.0	12.586581065195768	0.0
63	4.521204448865178E-4	0.0	0.0	12.872999367031378	0.0
64	4.521204448865178E-4	0.0	0.0	13.127543177502487	0.0
65	4.521204448865178E-4	0.0	0.0	13.343430689935799	0.0
66	4.521204448865178E-4	0.0	0.0	13.56542182837508	0.0
67	4.521204448865178E-4	0.0	0.0	13.782891762365495	0.0
68	4.521204448865178E-4	0.0	0.0	14.032010127497966	0.0
69	4.521204448865178E-4	0.0	0.0	14.28067637218555	0.0
70	4.521204448865178E-4	0.0	0.0	14.494981463061759	0.0
71	4.521204448865178E-4	0.0	0.0	14.710868975495073	0.0
72	4.521204448865178E-4	0.0	0.0	14.921104982367302	0.0
73	4.521204448865178E-4	0.0	0.0	15.142417940139254	0.0
74	4.521204448865178E-4	0.0	0.0	15.40781264128764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGG	25	1.2834935E-6	80.0	2
TCGTTAG	45	1.8189894E-12	80.0	1
CGAACGG	15	0.0013092555	80.0	2
CGAACCC	15	0.0013092555	80.0	6
GATCGGA	30	4.0543455E-8	80.0	9
GATCGCA	20	4.0881147E-5	80.0	9
GTCGAAG	20	4.0881147E-5	80.0	1
GCGAACG	15	0.0013092555	80.0	1
CGACCGG	15	0.0013092555	80.0	2
ATATGCG	15	0.0013092555	80.0	1
TATTACG	15	0.0013092555	80.0	1
GCGATAT	15	0.0013092555	80.0	9
GACGATA	30	4.0543455E-8	80.0	1
ATAGCGG	135	0.0	77.03703	2
GCGGGAT	250	0.0	75.2	5
TCGTAGG	50	3.6379788E-12	72.0	2
AAATGCG	45	1.1641532E-10	71.111115	1
TGTTGCG	40	3.7071004E-9	70.0	1
CGGGATC	120	0.0	70.0	6
TACGGGA	115	0.0	69.56521	4
>>END_MODULE