FastQCFastQC Report
Thu 26 May 2016
SRR1547280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547280_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3099022
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427251.3786607516823048No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC39980.12900844201815928TruSeq Adapter, Index 27 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT181500.074.8870541
ATAGCGG5550.065.585592
TAGCGGG17900.064.8044743
CGGGTAT2600.064.615386
CGAGGGA20650.064.116234
CGATAGG5200.063.076922
TAGGGAC31200.062.8205155
GTAGGGT15650.062.3642164
TAGGGAT46450.061.7438165
TCGTTAG1300.061.538461
TACGGGA11200.061.4285744
GTAGGGA44050.060.658344
TAGGGTA16450.060.0607955
ATAGGGA38050.060.0262834
TATAGCG2200.060.01
AGGGATT45150.059.9778566
AGGGATC29750.059.8319326
GGGCGAT15400.059.2207767
AGTAGGG48750.058.9948733
AGCGGGA20250.058.6666684
Produced by FastQC (version 0.11.5)