##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547280_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3099022
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97539836761404	33.0	31.0	34.0	30.0	34.0
2	32.38738705307674	33.0	31.0	34.0	31.0	34.0
3	32.45995575378297	34.0	31.0	34.0	31.0	34.0
4	36.04122010105124	37.0	35.0	37.0	35.0	37.0
5	36.019446457624376	37.0	35.0	37.0	35.0	37.0
6	36.071880419048334	37.0	35.0	37.0	35.0	37.0
7	36.29995172670604	37.0	36.0	37.0	35.0	37.0
8	36.28181568249596	37.0	37.0	37.0	35.0	37.0
9	38.02819437874271	39.0	39.0	39.0	35.0	39.0
10-11	37.65346309254984	39.0	37.5	39.0	35.0	39.0
12-13	37.088338191855364	39.0	35.0	39.0	33.5	39.0
14-15	38.202616341542594	40.0	37.0	41.0	33.0	41.0
16-17	38.366251352846156	40.0	37.0	41.0	34.0	41.0
18-19	38.17998452414987	40.0	36.0	41.0	34.0	41.0
20-21	37.943879552968646	40.0	35.0	41.0	34.0	41.0
22-23	37.77798140832817	40.0	35.0	41.0	33.5	41.0
24-25	37.70042903858056	40.0	35.0	41.0	33.5	41.0
26-27	37.657731213266636	40.0	35.0	41.0	33.5	41.0
28-29	37.54683009672084	40.0	35.0	41.0	33.0	41.0
30-31	37.3398204336723	40.0	35.0	41.0	33.0	41.0
32-33	36.94894131116204	40.0	35.0	41.0	31.5	41.0
34-35	36.54402647028643	39.0	35.0	41.0	31.0	41.0
36-37	36.34926309009745	39.0	35.0	41.0	30.0	41.0
38-39	36.23188493014894	39.0	35.0	41.0	30.0	41.0
40-41	36.07567984351192	39.0	35.0	41.0	30.0	41.0
42-43	36.00074733254556	39.0	35.0	41.0	29.5	41.0
44-45	35.87097235837629	39.0	35.0	41.0	29.0	41.0
46-47	35.7309104291612	38.0	35.0	41.0	29.0	41.0
48-49	35.60657071811688	38.0	35.0	40.0	28.0	41.0
50-51	35.37877514260951	38.0	35.0	40.0	27.5	41.0
52-53	35.19348281490096	37.5	35.0	40.0	27.0	41.0
54-55	34.96932516129282	37.0	34.5	40.0	26.0	41.0
56-57	34.66380893714211	37.0	34.0	40.0	25.5	41.0
58-59	34.380489554446534	36.0	34.0	40.0	25.0	41.0
60-61	33.957579358907424	35.5	34.0	39.0	24.0	41.0
62-63	33.51157510337132	35.0	33.0	39.0	22.0	40.5
64-65	32.97132256563522	35.0	33.0	38.5	19.5	40.0
66-67	32.462089975482584	35.0	32.5	37.5	17.5	40.0
68-69	31.820735057705303	35.0	31.5	37.0	13.0	39.5
70-71	31.417861667326015	35.0	31.0	36.5	11.5	39.0
72-73	31.071934145675634	35.0	31.0	36.0	10.5	39.0
74-75	30.726360122645147	35.0	31.0	35.5	10.0	37.5
76-77	28.87271645699837	32.5	27.5	34.5	10.0	36.0
78-79	29.84001952874165	34.0	29.5	35.0	10.0	36.0
80-81	29.858198328375856	34.0	30.0	35.0	10.0	36.0
82-83	29.59002517568446	34.0	29.0	35.0	9.0	36.0
84-85	29.309708353151414	34.0	29.0	35.0	8.0	35.5
86	28.89887067597455	34.0	28.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	18.0
10	19.0
11	30.0
12	56.0
13	149.0
14	440.0
15	1439.0
16	3600.0
17	8696.0
18	19491.0
19	32370.0
20	36823.0
21	34707.0
22	29496.0
23	25681.0
24	25974.0
25	28589.0
26	33757.0
27	41056.0
28	51444.0
29	64544.0
30	83367.0
31	103595.0
32	119619.0
33	140702.0
34	292694.0
35	291878.0
36	272202.0
37	447806.0
38	626462.0
39	281696.0
40	619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.630003917364895	23.423163823941877	28.452331090260092	15.494501168433139
2	33.98013954079707	23.557173843877198	28.953811880006015	13.508874735319724
3	29.230479809436655	23.601219997792853	33.79001504345565	13.378285149314847
4	25.919757910721515	26.222046826385874	32.97850095933491	14.879694303557702
5	23.52874552036094	30.36303065934995	31.12236699190906	14.98585682838005
6	22.27780248091172	38.32438104666569	28.575014956331383	10.822801516091205
7	82.57343768453403	4.072123398930373	10.558621397331159	2.7958175192044457
8	82.14585117498359	3.72811164296349	10.799407038736737	3.326630143316182
9	76.97241258693872	6.350680956766361	12.639406883849164	4.037499572445759
10-11	35.91765402117184	27.235705328971527	23.683584692202896	13.163055957653738
12-13	27.308889707785227	24.30954023559691	30.80789681389806	17.5736732427198
14-15	17.263462472999546	29.698869514317742	35.758329563326754	17.279338449355958
16-17	21.72761277590156	27.381138307504756	32.22195582993603	18.669293086657664
18-19	22.858889030152092	28.379195113813328	30.399929397080754	18.361986458953826
20-21	23.947619603862123	28.811750933036294	30.380019890145988	16.8606095729556
22-23	20.77010101896663	25.54660792985658	32.917594647601724	20.765696403575063
24-25	20.106198019891437	27.61805175955511	33.16212340538402	19.113626815169432
26-27	18.562904684122927	31.283095118395416	30.320242966974742	19.833757230506915
28-29	17.90359990990706	28.03624820991913	33.15504052568843	20.905111354485385
30-31	21.13942721284328	28.08229499500165	30.348751961102565	20.429525831052505
32-33	22.50521293491947	27.993557322277802	29.14106450357564	20.360165239227086
34-35	18.29977328331325	28.227808644146446	31.440483481562893	22.03193459097741
36-37	20.582170762259835	29.456276851213058	29.932701349006237	20.02885103752087
38-39	19.67591711191466	29.216410854779344	29.370394918138693	21.7372771151673
40-41	19.988289854024917	27.228251364462725	30.089121664834906	22.694337116677453
42-43	19.995066185396553	27.246531325043836	29.47920343902044	23.27919905053917
44-45	19.620464133523416	29.26100556885366	28.247024383821735	22.871505913801194
46-47	19.635194587195574	28.63795416747606	29.50011326153864	22.226737983789725
48-49	20.149986027850076	26.834691718871305	30.934969161238612	22.080353092040006
50-51	17.95637462399428	28.600151918895705	30.209111132479862	23.234362324630155
52-53	20.080431826556893	26.17943660935611	31.21741310645746	22.522718457629537
54-55	19.96749297036291	26.40920264522162	31.27368892508669	22.34961545932878
56-57	19.821527565793335	26.808618977212813	30.18679764132039	23.183055815673463
58-59	21.23465080273712	29.022107619758753	29.072139533052688	20.67110204445144
60-61	21.374743386784605	30.64229618247305	27.45208004331689	20.53088038742545
62-63	19.597811825795365	29.48134927728813	29.570103084134285	21.350735812782226
64-65	18.59281734689202	32.69153945986831	28.259802608693967	20.455840584545708
66-67	18.484508983801987	31.719022969181893	26.635031955242656	23.161436091773467
68-69	17.416672098487844	32.6548827339722	27.244659766855477	22.683785400684474
70-71	18.19887048236508	31.351019773334944	28.25999621816173	22.190113526138248
72-73	19.35141150982471	31.93875358096845	27.262262094299427	21.44757281490741
74-75	18.530426695905998	32.16142060301605	26.74514734003179	22.563005361046162
76-77	20.417747921763706	30.925304821972865	27.053970575233087	21.602976681030338
78-79	17.813394032052692	31.701630385328016	28.2203062772707	22.26466930534859
80-81	16.907688941866176	30.896295670053327	28.64745716551867	23.548558222561827
82-83	17.735288745933396	30.571919140941883	28.860782530746796	22.832009582377925
84-85	16.92879237385214	30.44060351943291	27.953496296573565	24.677107810141393
86	17.32562724627318	30.200753657121503	29.27878537164305	23.194833724962262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1203.0
1	1723.0
2	13477.5
3	15172.5
4	6395.5
5	9128.5
6	13074.0
7	15049.0
8	15691.5
9	15318.5
10	13318.5
11	12001.0
12	11575.0
13	11078.5
14	10675.0
15	10619.5
16	10784.5
17	10937.5
18	10963.5
19	11453.0
20	12028.5
21	12208.0
22	13002.5
23	14948.0
24	16639.0
25	17825.5
26	20232.0
27	26544.0
28	32062.5
29	33029.0
30	36706.5
31	42785.0
32	47487.5
33	54470.5
34	64177.0
35	73574.0
36	77946.0
37	80684.0
38	89842.5
39	102326.5
40	116502.5
41	133995.5
42	147181.0
43	150986.0
44	153312.5
45	153191.5
46	155054.5
47	156605.0
48	153395.0
49	142999.5
50	133054.0
51	125121.5
52	109877.5
53	99142.0
54	91802.0
55	86248.0
56	78627.5
57	72645.0
58	64663.5
59	49319.5
60	37063.0
61	28689.0
62	22754.0
63	17152.0
64	14005.0
65	11548.0
66	7399.0
67	4945.0
68	3966.5
69	3534.0
70	2567.5
71	1817.0
72	1430.0
73	909.0
74	595.5
75	306.5
76	147.5
77	94.0
78	72.5
79	55.0
80	51.5
81	34.0
82	19.0
83	17.0
84	13.0
85	8.0
86	6.0
87	3.5
88	2.5
89	2.5
90	3.5
91	4.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3099022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.13553258716107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14983035872694	16.30602753640822
2	9.026289399576138	3.8155086749177594
3	3.310601156805398	2.0991395589826087
4	1.7231635128443878	1.45679914314918
5	1.0904580386428282	1.1523705705333618
6	0.7534752720834569	0.9555060700043974
7	0.5718835011548432	0.8460943662302567
8	0.444022469727735	0.7507721102689826
9	0.3547282897223574	0.6747634194313319
>10	3.3018548822087013	16.15214339178686
>50	1.1579109892370236	17.738486238002142
>100	1.1097325622108978	35.08205089243296
>500	0.003102342081682816	0.4300385637171235
>1k	0.0027921078735145344	1.1395677697829911
>5k	0.0	0.0
>10k+	1.5511710408414077E-4	1.4007316943519175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42725	1.3786607516823048	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3998	0.12900844201815928	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.45364892537065E-5	0.0	0.0	0.13972149923427454	0.0
2	6.45364892537065E-5	0.0	0.0	0.42274627285640437	0.0
3	6.45364892537065E-5	0.0	0.0	0.6232934132122973	0.0
4	6.45364892537065E-5	0.0	0.0	0.9617872993479879	0.0
5	6.45364892537065E-5	0.0	0.0	1.8163472218009424	0.0
6	6.45364892537065E-5	0.0	0.0	2.619245684606305	0.0
7	6.45364892537065E-5	0.0	0.0	3.204365764425035	0.0
8	6.45364892537065E-5	0.0	0.0	4.145533655456464	0.0
9	6.45364892537065E-5	0.0	0.0	4.487093024831705	0.0
10	6.45364892537065E-5	0.0	0.0	4.9958664378633	0.0
11	6.45364892537065E-5	0.0	0.0	5.707897523799444	0.0
12	6.45364892537065E-5	0.0	0.0	6.200859496963881	0.0
13	6.45364892537065E-5	0.0	0.0	6.399728688599177	0.0
14	6.45364892537065E-5	0.0	0.0	6.493919694664962	0.0
15	6.45364892537065E-5	0.0	0.0	6.632350464114163	0.0
16	6.45364892537065E-5	0.0	0.0	6.899434724890627	0.0
17	6.45364892537065E-5	0.0	0.0	7.238832121875869	0.0
18	6.45364892537065E-5	0.0	0.0	7.6001073887181185	0.0
19	6.45364892537065E-5	0.0	0.0	7.841215712569966	0.0
20	6.45364892537065E-5	0.0	0.0	8.096554332302256	0.0
21	6.45364892537065E-5	0.0	0.0	8.404361117797809	0.0
22	6.45364892537065E-5	0.0	0.0	8.74149973765917	0.0
23	6.45364892537065E-5	0.0	0.0	9.078606089275906	0.0
24	9.680473388055973E-5	0.0	0.0	9.332428101510734	0.0
25	1.29072978507413E-4	0.0	0.0	9.566243802076913	0.0
26	1.29072978507413E-4	0.0	0.0	9.782408772832204	0.0
27	1.6134122313426623E-4	0.0	0.0	10.018547787011515	0.0
28	1.6134122313426623E-4	0.0	0.0	10.262302106922764	0.0
29	1.6134122313426623E-4	0.0	0.0	10.546133586660567	0.0
30	1.6134122313426623E-4	0.0	0.0	10.851584790298359	0.0
31	1.6134122313426623E-4	0.0	0.0	11.12002431734915	0.0
32	1.6134122313426623E-4	0.0	0.0	11.3742335485195	0.0
33	1.6134122313426623E-4	0.0	0.0	11.617665185984482	0.0
34	1.6134122313426623E-4	0.0	0.0	11.868228105511998	0.0
35	1.6134122313426623E-4	0.0	0.0	12.14434747478398	0.0
36	1.6134122313426623E-4	0.0	0.0	12.385036311455679	0.0
37	1.6134122313426623E-4	0.0	0.0	12.644343925277072	0.0
38	1.6134122313426623E-4	0.0	0.0	12.94902069104382	0.0
39	1.6134122313426623E-4	0.0	0.0	13.426397102053487	0.0
40	1.6134122313426623E-4	0.0	0.0	13.733397181433368	0.0
41	1.6134122313426623E-4	0.0	0.0	14.01464720160102	0.0
42	1.6134122313426623E-4	0.0	0.0	14.299446728677628	0.0
43	1.6134122313426623E-4	0.0	0.0	14.5631750920129	0.0
44	1.9360946776111947E-4	0.0	0.0	14.813221719626386	0.0
45	1.9360946776111947E-4	0.0	0.0	15.073465112541957	0.0
46	2.2587771238797273E-4	0.0	0.0	15.345131464055434	0.0
47	2.2587771238797273E-4	0.0	0.0	15.653970833379047	0.0
48	2.58145957014826E-4	0.0	0.0	15.927863693771776	0.0
49	2.58145957014826E-4	0.0	0.0	16.205499670541222	0.0
50	2.58145957014826E-4	0.0	0.0	16.495558921492005	0.0
51	2.58145957014826E-4	0.0	0.0	16.792039553123534	0.0
52	2.904142016416792E-4	0.0	0.0	17.14463466216116	0.0
53	2.904142016416792E-4	0.0	0.0	17.439308272093584	0.0
54	2.904142016416792E-4	0.0	0.0	17.72933525479974	0.0
55	2.904142016416792E-4	0.0	0.0	18.017200265115896	0.0
56	2.904142016416792E-4	0.0	0.0	18.29996689278101	0.0
57	2.904142016416792E-4	0.0	0.0	18.57934535476031	0.0
58	2.904142016416792E-4	0.0	0.0	18.85865928025035	0.0
59	2.904142016416792E-4	0.0	0.0	19.14426551344263	0.0
60	2.904142016416792E-4	0.0	0.0	19.472336756563845	0.0
61	2.904142016416792E-4	0.0	0.0	19.781531076578354	0.0
62	2.904142016416792E-4	0.0	0.0	20.137546619546423	0.0
63	2.904142016416792E-4	0.0	0.0	20.486398612207335	0.0
64	3.2268244626853246E-4	0.0	0.0	20.80036863242662	0.0
65	3.2268244626853246E-4	0.0	0.0	21.10194764670919	0.0
66	3.549506908953857E-4	0.0	0.0	21.395524136324298	0.0
67	3.549506908953857E-4	0.0	0.0	21.690875379393887	0.0
68	3.549506908953857E-4	0.0	0.0	22.04379317087778	0.0
69	3.8721893552223893E-4	0.0	0.0	22.556567846243105	0.0
70	3.8721893552223893E-4	0.0	0.0	22.8855748684585	0.0
71	3.8721893552223893E-4	0.0	0.0	23.196640746661366	0.0
72	3.8721893552223893E-4	0.0	0.0	23.49221786744334	0.0
73	3.8721893552223893E-4	0.0	0.0	23.797023706188597	0.0
74	4.194871801490922E-4	0.0	0.0	24.127256921699814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18150	0.0	74.887054	1
ATAGCGG	555	0.0	65.58559	2
TAGCGGG	1790	0.0	64.804474	3
CGGGTAT	260	0.0	64.61538	6
CGAGGGA	2065	0.0	64.11623	4
CGATAGG	520	0.0	63.07692	2
TAGGGAC	3120	0.0	62.820515	5
GTAGGGT	1565	0.0	62.364216	4
TAGGGAT	4645	0.0	61.743816	5
TCGTTAG	130	0.0	61.53846	1
TACGGGA	1120	0.0	61.428574	4
GTAGGGA	4405	0.0	60.65834	4
TAGGGTA	1645	0.0	60.060795	5
ATAGGGA	3805	0.0	60.026283	4
TATAGCG	220	0.0	60.0	1
AGGGATT	4515	0.0	59.977856	6
AGGGATC	2975	0.0	59.831932	6
GGGCGAT	1540	0.0	59.220776	7
AGTAGGG	4875	0.0	58.994873	3
AGCGGGA	2025	0.0	58.666668	4
>>END_MODULE