FastQCFastQC Report
Thu 26 May 2016
SRR1547279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547279_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1186241
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197081.6613824678121898No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC15200.1281358509780053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG404.1836756E-1180.01
CGTAAGG2700.077.037032
CGTTTTT80200.075.7107241
ATTACGG1800.068.8888852
TAGCGCG351.1815246E-768.571431
CGTTCGA351.1815246E-768.571436
CGGGACG700.068.571436
CGGGATC2700.068.148156
CGCGAGG1200.066.6666642
CGAGGGA6650.066.165424
GGCGATA1150.066.086968
TCGAAAG1150.066.086961
TTACGGG4950.065.454553
GGTTACG559.094947E-1265.4545441
ACGGGAT5750.064.6956565
GCGAGGG7200.064.444443
TACGGGA5350.064.2990654
TTAGGGA16450.063.4650424
AGGGCGA4350.063.4482776
ATTAGCG1200.063.3333321
Produced by FastQC (version 0.11.5)