##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547279_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1186241
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01952891528787	33.0	31.0	34.0	31.0	34.0
2	32.432857235587036	34.0	31.0	34.0	31.0	34.0
3	32.49706256991623	34.0	31.0	34.0	31.0	34.0
4	36.065329052022314	37.0	35.0	37.0	35.0	37.0
5	36.04659761380697	37.0	35.0	37.0	35.0	37.0
6	36.099630682129515	37.0	36.0	37.0	35.0	37.0
7	36.33573616153885	37.0	37.0	37.0	35.0	37.0
8	36.33113085789481	37.0	37.0	37.0	35.0	37.0
9	38.11846580922426	39.0	39.0	39.0	37.0	39.0
10-11	37.650332436663376	39.0	37.5	39.0	35.0	39.0
12-13	36.96676097015699	39.0	35.0	39.0	33.5	39.0
14-15	38.15180262695355	40.0	36.0	41.0	33.0	41.0
16-17	38.30422485818649	40.0	36.0	41.0	34.0	41.0
18-19	38.10439573408776	40.0	36.0	41.0	34.0	41.0
20-21	37.82748067213998	40.0	35.0	41.0	34.0	41.0
22-23	37.62230019026488	39.5	35.0	41.0	33.0	41.0
24-25	37.56519585817722	39.0	35.0	41.0	33.0	41.0
26-27	37.49146084143104	39.0	35.0	41.0	33.5	41.0
28-29	37.3754527958484	39.0	35.0	41.0	33.0	41.0
30-31	37.18648234212104	39.0	35.0	41.0	33.0	41.0
32-33	36.7366850412353	39.0	35.0	41.0	32.0	41.0
34-35	36.31468605452012	39.0	35.0	41.0	30.5	41.0
36-37	36.06206495981845	39.0	35.0	41.0	30.0	41.0
38-39	35.97263203682894	39.0	35.0	41.0	30.0	41.0
40-41	35.85992053891242	39.0	35.0	41.0	29.0	41.0
42-43	35.80004274005029	38.5	35.0	41.0	29.0	41.0
44-45	35.66841223663657	38.0	35.0	41.0	28.5	41.0
46-47	35.53986710963455	38.0	35.0	41.0	28.0	41.0
48-49	35.446410130825015	38.0	35.0	40.0	28.0	41.0
50-51	35.23166413907461	37.5	35.0	40.0	27.0	41.0
52-53	35.0524332745201	37.0	35.0	40.0	26.5	41.0
54-55	34.87732551817042	37.0	34.5	40.0	26.0	41.0
56-57	34.59110922653997	36.0	34.0	40.0	25.5	41.0
58-59	34.35366506468753	36.0	34.0	40.0	25.0	41.0
60-61	33.99761557727308	35.5	34.0	39.0	24.0	41.0
62-63	33.56841105643794	35.0	33.5	39.0	22.5	40.0
64-65	33.18407853041667	35.0	33.0	38.5	21.5	40.0
66-67	32.83360969651192	35.0	33.0	37.5	20.0	40.0
68-69	32.34363927734752	35.0	32.5	37.0	18.5	39.5
70-71	31.992776341401118	35.0	32.0	36.5	17.5	39.0
72-73	31.690308714670962	35.0	32.0	36.0	15.0	39.0
74-75	31.387755523540328	35.0	32.0	35.5	14.0	38.0
76-77	29.453466454118512	33.0	29.0	34.5	10.5	36.0
78-79	30.416192830967738	34.0	30.5	35.0	11.0	36.0
80-81	30.56088686868857	34.5	31.0	35.0	10.0	36.0
82-83	30.358220631389404	34.0	31.0	35.0	10.0	36.0
84-85	30.139479245785637	34.0	31.0	35.0	10.0	36.0
86	29.716855175297432	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	4.0
11	7.0
12	12.0
13	45.0
14	184.0
15	574.0
16	1523.0
17	3892.0
18	8700.0
19	14016.0
20	15259.0
21	13890.0
22	11321.0
23	9584.0
24	9350.0
25	10011.0
26	11560.0
27	13978.0
28	16988.0
29	20879.0
30	26304.0
31	32891.0
32	39991.0
33	49984.0
34	123239.0
35	126726.0
36	97267.0
37	165533.0
38	251188.0
39	111074.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.198044916673762	23.604141148383846	29.610509162977845	15.58730477196455
2	33.38739767045651	25.32689394482234	29.06171680122336	12.223991583497789
3	27.928473219185644	24.54484375434671	34.612359545825846	12.914323480641793
4	24.481113028465547	26.461570625193364	35.037821151014	14.019495195327089
5	23.855354856222302	31.214904897065605	32.07611269548094	12.853627551231158
6	22.377071775465527	39.59574825014479	27.731211448601083	10.295968525788604
7	85.08254224900337	3.4293200117008267	9.987093685010045	1.501044054285765
8	85.70400112624668	3.1851874956269426	9.577480461390223	1.533330916736144
9	81.40774092279732	5.685691187541149	10.432871566570368	2.4736963230911764
10-11	39.09740937971289	26.795904036363606	22.227734499144777	11.878952084778724
12-13	26.920667891263246	27.1611333616019	29.772112075033657	16.1460866721012
14-15	16.219554036658657	32.96235756477815	35.32423006792043	15.493858330642762
16-17	20.147086468938436	27.47342234840981	33.46305683246491	18.91643435018685
18-19	22.17926205551823	29.241781391808242	31.2349682737319	17.343988278941634
20-21	24.0382856434738	29.290759634846548	30.93869626829624	15.73225845338342
22-23	20.45237856388373	26.866041554793675	32.18603976763575	20.495540113686847
24-25	18.20797797412162	29.41324739239328	34.5446667245526	17.834107908932502
26-27	17.90820752275465	34.667323081903255	30.2052449713001	17.219224424041997
28-29	16.49921896140835	29.46083468704926	35.8230325878131	18.21691376372929
30-31	20.572000124763857	30.69068595673223	30.974608026530863	17.762705891973045
32-33	23.312337037752027	30.57114869575407	29.01788085220457	17.09863341428934
34-35	18.50450287926315	31.629786864557875	29.922418800226936	19.943291455952036
36-37	21.900524429690087	31.416634562454004	29.424838628912674	17.25800237894323
38-39	19.054559739547024	32.885012404730574	28.76654069451317	19.29388716120923
40-41	19.52988473674405	30.620674888155104	28.404725515304225	21.44471485979662
42-43	20.619334519713952	29.46370088371587	28.6046427328005	21.31232186376967
44-45	18.65295500661333	33.522277513591256	27.747397029777254	20.07737045001817
46-47	20.305401684817838	32.110043406019514	27.93555441095022	19.64900049821242
48-49	20.457267958197363	29.445112755333863	30.1409662960562	19.95665299041257
50-51	18.626274087643235	33.03826962649242	28.025165206732865	20.310291079131478
52-53	20.046432385999136	29.89114353660007	29.85750787571834	20.204916201682458
54-55	20.605720085547542	29.955422211843967	30.509399017568946	18.92945868503955
56-57	20.882729563385517	28.547445249321175	29.434617417539943	21.135207769753364
58-59	21.497528748374066	30.489462090755588	28.211004340601953	19.802004820268394
60-61	22.015172296354617	32.05899981538322	26.562182558181686	19.36364533008048
62-63	21.154259547596148	30.107836434586225	29.548211535429985	19.189692482387642
64-65	18.475040063528407	33.69100376736262	29.34926376680624	18.484692402302734
66-67	17.796257252952817	32.64796108042126	26.06717353387718	23.48860813274874
68-69	17.882201003000233	34.480345899357715	25.32651459526353	22.310938502378523
70-71	18.530003599605813	33.03843822629634	27.426678052773422	21.00488012132442
72-73	20.78068453206389	32.66351441233274	26.662288691758253	19.89351236384512
74-75	19.92141563139362	34.11018502985481	24.998250777034347	20.970148561717224
76-77	23.352885290594408	31.90818729077818	25.496041698103504	19.242885720523905
78-79	18.552132323870108	32.76914218948763	28.201857801239377	20.476867685402883
80-81	16.48404497905569	31.97811405945335	29.675883736947213	21.861957224543747
82-83	18.155374835299067	30.269987296004775	30.423160217864663	21.15147765083149
84-85	16.6609483233171	30.53730228511744	28.729659487406018	24.07208990415944
86	18.018513944468282	30.368871081002936	29.2524031794551	22.36021179507368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	459.0
1	742.0
2	6568.5
3	7213.5
4	2742.0
5	4364.5
6	6846.5
7	8133.0
8	8579.5
9	8290.0
10	6991.0
11	5951.5
12	5184.5
13	4887.0
14	4880.0
15	4914.5
16	4977.5
17	4971.5
18	5040.0
19	5116.5
20	5356.0
21	5622.0
22	5731.0
23	6241.0
24	7289.0
25	7999.5
26	8424.5
27	9922.5
28	11742.5
29	12426.0
30	14361.5
31	17730.5
32	20785.0
33	23434.5
34	26087.5
35	30615.5
36	33519.0
37	34095.0
38	36402.5
39	39426.5
40	43232.0
41	49835.0
42	57456.0
43	60987.0
44	62408.5
45	62283.5
46	62931.5
47	64335.0
48	62241.5
49	56064.5
50	51190.0
51	46136.5
52	38191.5
53	33123.5
54	29189.0
55	26495.0
56	22829.0
57	20099.0
58	18915.5
59	15285.0
60	10964.5
61	8018.0
62	5807.5
63	3663.5
64	2657.0
65	2329.5
66	1678.5
67	1142.0
68	780.5
69	544.0
70	385.0
71	314.0
72	272.0
73	149.0
74	87.0
75	75.5
76	52.0
77	35.0
78	10.5
79	10.0
80	13.0
81	9.5
82	3.0
83	3.0
84	3.0
85	3.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1186241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.639433302113645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89820237000042	18.414693592801758
2	7.971433425485844	3.7688033756802373
3	2.999002335583985	2.1268414705476193
4	1.6573658717214754	1.56716759927037
5	1.0479287416807053	1.2386220797164453
6	0.762313182009211	1.0812390972857255
7	0.5623954432289404	0.9306296698736224
8	0.437468321183324	0.8273202560320653
9	0.3389890403425189	0.7212157928392039
>10	4.198856449434936	23.28312971935862
>50	1.543335065794596	26.491595448198563
>100	0.5791105580211872	17.318122400960416
>500	0.0028793564102979944	0.4244787989741341
>1k	3.599195512872493E-4	0.12932607224708034
>5k	0.0	0.0
>10k+	3.599195512872493E-4	1.6768146262141177
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19708	1.6613824678121898	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	1520	0.1281358509780053	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05850413195969453	0.0
2	8.429990195921402E-5	0.0	0.0	0.1869771825455367	0.0
3	8.429990195921402E-5	0.0	0.0	0.281645972445734	0.0
4	8.429990195921402E-5	0.0	0.0	0.4267261037175414	0.0
5	8.429990195921402E-5	0.0	0.0	0.8180462486122129	0.0
6	8.429990195921402E-5	0.0	0.0	1.154402857429477	0.0
7	8.429990195921402E-5	0.0	0.0	1.4091571611502216	0.0
8	8.429990195921402E-5	0.0	0.0	1.833101368103109	0.0
9	8.429990195921402E-5	0.0	0.0	2.0038086695705175	0.0
10	8.429990195921402E-5	0.0	0.0	2.3238110974076935	0.0
11	8.429990195921402E-5	0.0	0.0	2.7778503693600203	0.0
12	8.429990195921402E-5	0.0	0.0	3.087736808962091	0.0
13	8.429990195921402E-5	0.0	0.0	3.195640683469885	0.0
14	8.429990195921402E-5	0.0	0.0	3.2383807337632065	0.0
15	8.429990195921402E-5	0.0	0.0	3.3096984508207017	0.0
16	8.429990195921402E-5	0.0	0.0	3.454778582092509	0.0
17	8.429990195921402E-5	0.0	0.0	3.64234586395176	0.0
18	8.429990195921402E-5	0.0	0.0	3.8558775156144494	0.0
19	1.6859980391842805E-4	0.0	0.0	4.000114647866664	0.0
20	1.6859980391842805E-4	0.0	0.0	4.144267480216921	0.0
21	1.6859980391842805E-4	0.0	0.0	4.324163470997883	0.0
22	1.6859980391842805E-4	0.0	0.0	4.534576026288081	0.0
23	1.6859980391842805E-4	0.0	0.0	4.746421679911586	0.0
24	1.6859980391842805E-4	0.0	0.0	4.90364099706552	0.0
25	1.6859980391842805E-4	0.0	0.0	5.056392419415616	0.0
26	1.6859980391842805E-4	0.0	0.0	5.189923464119011	0.0
27	1.6859980391842805E-4	0.0	0.0	5.3335704970575115	0.0
28	1.6859980391842805E-4	0.0	0.0	5.485900419897812	0.0
29	1.6859980391842805E-4	0.0	0.0	5.663098813816079	0.0
30	1.6859980391842805E-4	0.0	0.0	5.848137098616554	0.0
31	1.6859980391842805E-4	0.0	0.0	6.036463079593439	0.0
32	1.6859980391842805E-4	0.0	0.0	6.210795276845093	0.0
33	1.6859980391842805E-4	0.0	0.0	6.393388864488751	0.0
34	1.6859980391842805E-4	0.0	0.0	6.571598857230529	0.0
35	1.6859980391842805E-4	0.0	0.0	6.780072514775665	0.0
36	1.6859980391842805E-4	0.0	0.0	6.968229895948631	0.0
37	1.6859980391842805E-4	0.0	0.0	7.176534953689849	0.0
38	1.6859980391842805E-4	0.0	0.0	7.396726297607316	0.0
39	1.6859980391842805E-4	0.0	0.0	7.6627767881905955	0.0
40	1.6859980391842805E-4	0.0	0.0	7.883305331715899	0.0
41	2.5289970587764204E-4	0.0	0.0	8.1170689598488	0.0
42	2.5289970587764204E-4	0.0	0.0	8.338356202491736	0.0
43	2.5289970587764204E-4	0.0	0.0	8.554163951507324	0.0
44	2.5289970587764204E-4	0.0	0.0	8.763480608072053	8.429990195921402E-5
45	2.5289970587764204E-4	0.0	0.0	8.989488645224705	8.429990195921402E-5
46	2.5289970587764204E-4	0.0	0.0	9.216339681396951	8.429990195921402E-5
47	2.5289970587764204E-4	0.0	0.0	9.45979779825516	8.429990195921402E-5
48	2.5289970587764204E-4	0.0	0.0	9.699293819721287	8.429990195921402E-5
49	2.5289970587764204E-4	0.0	0.0	9.936766643540393	8.429990195921402E-5
50	2.5289970587764204E-4	0.0	0.0	10.183259556869135	8.429990195921402E-5
51	2.5289970587764204E-4	0.0	0.0	10.429668170295917	8.429990195921402E-5
52	2.5289970587764204E-4	0.0	0.0	10.697320359016423	8.429990195921402E-5
53	2.5289970587764204E-4	0.0	0.0	10.944066172051041	8.429990195921402E-5
54	2.5289970587764204E-4	0.0	0.0	11.21348865871269	8.429990195921402E-5
55	2.5289970587764204E-4	0.0	0.0	11.46984466057066	8.429990195921402E-5
56	2.5289970587764204E-4	0.0	0.0	11.736148050859818	8.429990195921402E-5
57	2.5289970587764204E-4	0.0	0.0	12.000006743992158	8.429990195921402E-5
58	2.5289970587764204E-4	0.0	0.0	12.24742695624245	8.429990195921402E-5
59	2.5289970587764204E-4	0.0	0.0	12.510695550061076	8.429990195921402E-5
60	2.5289970587764204E-4	0.0	0.0	12.784080132114806	8.429990195921402E-5
61	2.5289970587764204E-4	0.0	0.0	13.067243502795806	8.429990195921402E-5
62	2.5289970587764204E-4	0.0	0.0	13.37772004171159	8.429990195921402E-5
63	2.5289970587764204E-4	0.0	0.0	13.686173382980355	8.429990195921402E-5
64	2.5289970587764204E-4	0.0	0.0	13.992013427288384	8.429990195921402E-5
65	2.5289970587764204E-4	0.0	0.0	14.27559829747918	8.429990195921402E-5
66	2.5289970587764204E-4	0.0	0.0	14.53726519316058	8.429990195921402E-5
67	2.5289970587764204E-4	0.0	0.0	14.819754164625907	8.429990195921402E-5
68	2.5289970587764204E-4	0.0	0.0	15.123149511777118	8.429990195921402E-5
69	2.5289970587764204E-4	0.0	0.0	15.49929567431913	8.429990195921402E-5
70	2.5289970587764204E-4	0.0	0.0	15.797717327254748	8.429990195921402E-5
71	2.5289970587764204E-4	0.0	0.0	16.096223280092325	8.429990195921402E-5
72	2.5289970587764204E-4	0.0	0.0	16.371715359695038	8.429990195921402E-5
73	2.5289970587764204E-4	0.0	0.0	16.65319273233685	8.429990195921402E-5
74	2.5289970587764204E-4	0.0	0.0	16.964090770762432	8.429990195921402E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	40	4.1836756E-11	80.0	1
CGTAAGG	270	0.0	77.03703	2
CGTTTTT	8020	0.0	75.710724	1
ATTACGG	180	0.0	68.888885	2
TAGCGCG	35	1.1815246E-7	68.57143	1
CGTTCGA	35	1.1815246E-7	68.57143	6
CGGGACG	70	0.0	68.57143	6
CGGGATC	270	0.0	68.14815	6
CGCGAGG	120	0.0	66.666664	2
CGAGGGA	665	0.0	66.16542	4
GGCGATA	115	0.0	66.08696	8
TCGAAAG	115	0.0	66.08696	1
TTACGGG	495	0.0	65.45455	3
GGTTACG	55	9.094947E-12	65.454544	1
ACGGGAT	575	0.0	64.695656	5
GCGAGGG	720	0.0	64.44444	3
TACGGGA	535	0.0	64.299065	4
TTAGGGA	1645	0.0	63.465042	4
AGGGCGA	435	0.0	63.448277	6
ATTAGCG	120	0.0	63.333332	1
>>END_MODULE