FastQCFastQC Report
Thu 26 May 2016
SRR1547278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547278_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3360203
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220470.6561210736375154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127950.072.559591
ACGGGAT20300.069.3596045
GTTAGCG2700.068.148151
TTAGCGG9700.068.041242
CGGGATT19950.065.964916
GGGCGAT23250.065.720437
TACGGGA14550.065.704474
GCGGGAT32950.065.553875
CGTAAGG4800.064.1666642
TTAGGGA65900.063.1866464
CGAGGGA24650.063.123734
CGGGATA11050.062.9864236
TAGGGGT57200.062.937065
CGGGATC13750.062.2545476
GTTTGCG2700.062.222221
AGGGATC57150.062.222226
CTACGGG9900.061.8181843
TAGCGGG23100.061.645023
CGGGAAT10850.061.566826
TAAGGGA51900.061.271684
Produced by FastQC (version 0.11.5)