##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547278_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3360203
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12191971735041	33.0	31.0	34.0	31.0	34.0
2	32.551559831355426	34.0	31.0	34.0	31.0	34.0
3	32.654363739333604	34.0	31.0	34.0	31.0	34.0
4	36.17045696346322	37.0	35.0	37.0	35.0	37.0
5	36.14629264958099	37.0	35.0	37.0	35.0	37.0
6	36.204246588673364	37.0	37.0	37.0	35.0	37.0
7	36.42028145323363	37.0	37.0	37.0	35.0	37.0
8	36.4244395353495	37.0	37.0	37.0	35.0	37.0
9	38.242575820567986	39.0	39.0	39.0	37.0	39.0
10-11	37.771216649708364	39.0	37.5	39.0	35.0	39.0
12-13	37.219734641032105	39.0	37.0	39.0	34.5	39.0
14-15	38.450355975516956	40.0	37.0	41.0	34.0	41.0
16-17	38.60058067920301	40.0	37.0	41.0	35.0	41.0
18-19	38.455237972229654	40.0	37.0	41.0	35.0	41.0
20-21	38.28556905639332	40.0	36.0	41.0	34.0	41.0
22-23	38.104156058428615	40.0	35.0	41.0	34.0	41.0
24-25	38.09014008975053	40.0	35.0	41.0	34.0	41.0
26-27	38.084397430750464	40.0	35.0	41.0	34.0	41.0
28-29	38.01397817334251	40.0	36.0	41.0	34.0	41.0
30-31	37.863887836538446	40.0	35.5	41.0	34.0	41.0
32-33	37.56055288921532	40.0	35.0	41.0	33.0	41.0
34-35	37.253017302823665	40.0	35.0	41.0	33.0	41.0
36-37	37.07929119163336	40.0	35.0	41.0	32.5	41.0
38-39	36.98215479838569	40.0	35.0	41.0	32.0	41.0
40-41	36.872591179759084	40.0	35.0	41.0	31.5	41.0
42-43	36.82936269028984	40.0	35.0	41.0	32.0	41.0
44-45	36.71900656002033	39.5	35.0	41.0	31.0	41.0
46-47	36.588256721394515	39.0	35.0	41.0	31.0	41.0
48-49	36.48520744133613	39.0	35.0	41.0	31.0	41.0
50-51	36.27088467571751	39.0	35.0	41.0	31.0	41.0
52-53	36.08346326099941	38.5	35.0	40.0	30.0	41.0
54-55	35.901195552768684	38.0	35.0	40.0	30.0	41.0
56-57	35.64037827476494	37.5	35.0	40.0	29.5	41.0
58-59	35.392613630783615	37.0	35.0	40.0	29.0	41.0
60-61	35.04622071940297	36.5	34.5	40.0	28.5	41.0
62-63	34.69219136462886	36.0	34.0	39.0	27.5	41.0
64-65	34.325086758151215	35.5	34.0	39.0	26.5	40.0
66-67	33.99808210992015	35.0	34.0	38.5	26.0	40.0
68-69	33.49648577779378	35.0	34.0	37.5	25.5	40.0
70-71	33.178383716698065	35.0	33.5	37.0	25.0	39.5
72-73	32.886865912565405	35.0	33.0	37.0	24.5	39.0
74-75	32.57818337165939	35.0	33.0	36.0	24.0	38.5
76-77	30.609705127934234	33.5	30.0	34.5	20.0	36.0
78-79	31.64220703332507	34.5	32.0	35.0	20.0	37.0
80-81	31.73688643215901	35.0	33.0	35.0	20.0	37.0
82-83	31.53610302710878	35.0	32.5	35.0	19.5	36.0
84-85	31.297532619309013	35.0	32.0	35.0	19.0	36.0
86	30.869293015927905	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	5.0
11	16.0
12	25.0
13	79.0
14	287.0
15	904.0
16	2585.0
17	6450.0
18	14882.0
19	24177.0
20	28013.0
21	27435.0
22	22658.0
23	19861.0
24	19249.0
25	20868.0
26	24484.0
27	29947.0
28	37083.0
29	47680.0
30	60768.0
31	77563.0
32	97450.0
33	126089.0
34	306781.0
35	308215.0
36	281193.0
37	525855.0
38	845520.0
39	403013.0
40	1058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.301948126348318	24.45373092042356	30.12627510897407	14.118045844254052
2	30.618090633214717	25.14098106572728	31.62683921179762	12.61408908926038
3	28.046281727621814	24.516792586638367	33.93553901356555	13.50138667217427
4	25.43286819278478	27.52295025032714	33.016011235035506	14.02817032185258
5	23.85674317890913	31.81727413492578	31.016429662136485	13.30955302402861
6	22.767314950912194	40.839854020724346	26.134313909010853	10.25851711935261
7	89.29820609052489	3.0443994008695308	6.42032639099483	1.2370681176107516
8	90.0898844504335	2.1817134262424025	6.348961654995248	1.379440468328848
9	85.95906259234934	5.0399633593565625	7.076001063031014	1.9249729852630928
10-11	36.853487720831154	30.442491123304155	21.357310852945492	11.346710302919199
12-13	27.12987876030109	25.397483425852545	29.668713467608953	17.803924346237416
14-15	16.219555782790504	31.274866429200856	34.73037194479024	17.7752058432184
16-17	22.440355538043384	27.711554926889836	31.73888601373191	18.10920352133487
18-19	22.643884908143942	27.912167806528355	31.332868877267238	18.111078408060465
20-21	25.117857462778293	27.81389993402184	31.03854142145579	16.02970118174408
22-23	21.238716827525003	24.34396076665606	33.336899586126194	21.08042281969274
24-25	18.844114477607455	28.749944571801166	33.98825904268284	18.41768190790854
26-27	18.602030889205206	32.971876996717164	30.195333436700107	18.23075867737753
28-29	17.117254523015426	28.363911347022785	35.33713588137383	19.18169824858796
30-31	20.718733362240314	28.892569883426685	31.627910575640815	18.76078617869218
32-33	23.18855438198228	28.41508385058879	29.953264728351233	18.4430970390777
34-35	19.611240749442814	29.220541139925178	30.555713449455286	20.612504661176718
36-37	21.519756395670143	29.444872824647796	30.389146131945004	18.646224647737057
38-39	20.14381273988506	30.15896658624494	29.50211638999191	20.195104283878088
40-41	20.27712313809612	28.4444719560098	29.751714405349915	21.526690500544163
42-43	20.331167491964028	27.972417142654777	29.675230930988395	22.021184434392804
44-45	19.46998440272805	30.57938761437925	29.10437256320526	20.84625541968744
46-47	20.21419539236171	30.072126594732524	29.144652867698767	20.569025145207
48-49	21.130404918988525	27.713429813615427	30.93775881992844	20.218406447467608
50-51	19.089099676418357	30.461820312641823	29.39588768892832	21.053192322011498
52-53	21.04923422781302	28.290790764724633	30.503930863700795	20.156044143761555
54-55	20.947439782655987	28.43857945487222	30.742011717744433	19.871969044727358
56-57	20.927277310329167	27.15623133483304	30.243634089964207	21.672857264873578
58-59	21.82089891592859	28.596694902063952	28.843926988934893	20.73847919307256
60-61	21.785796870010532	29.1617351689764	28.750807614897077	20.301660346115995
62-63	21.037642666231772	27.94737401282006	30.485792078633345	20.529191242314823
64-65	19.66729391051672	30.627598987323086	29.616112478918684	20.08899462324151
66-67	19.43431691478164	29.86646937699895	27.294392630445248	23.40482107777417
68-69	18.57369926757401	31.376422793503846	27.694398225345314	22.355479713576827
70-71	19.665359503577612	29.98384323804246	28.83793032742367	21.512866930956253
72-73	20.882294908968298	30.331352004625913	27.961004141714056	20.825348944691736
74-75	20.074754412159027	31.84087389958285	26.704889555779815	21.379482132478305
76-77	22.745441272446932	29.899949497098838	27.23847338985174	20.116135840602485
78-79	19.156030156511378	30.62155768565173	29.15680987130837	21.065602286528524
80-81	17.761233473096716	29.97182015491326	30.01994224753683	22.247004124453195
82-83	18.953081108492555	29.358270913989422	30.047217385378204	21.64143059213982
84-85	18.24624881294374	28.96533334444377	29.11657420697499	23.67184363563749
86	18.779549926001497	29.24992924534619	29.86712409934757	22.103396729304748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1040.0
1	1547.0
2	7356.0
3	8098.0
4	4038.0
5	5180.0
6	6870.0
7	7918.0
8	8399.5
9	9035.0
10	9028.5
11	8694.5
12	8290.0
13	8123.5
14	8188.0
15	8270.0
16	8665.5
17	9043.0
18	9465.0
19	10138.0
20	11138.0
21	11823.0
22	12543.5
23	14317.5
24	16576.5
25	20468.0
26	25240.5
27	30139.5
28	37991.5
29	43031.0
30	48590.5
31	57390.5
32	63888.5
33	71859.0
34	83554.0
35	98203.5
36	105871.0
37	110183.0
38	117245.5
39	128844.0
40	143703.5
41	155768.0
42	170619.0
43	179417.0
44	180663.5
45	184442.0
46	183589.0
47	183654.5
48	176722.5
49	152698.5
50	139057.0
51	126944.0
52	108215.5
53	95429.0
54	83253.5
55	75663.0
56	68294.5
57	62512.0
58	55489.5
59	41056.0
60	28410.0
61	20654.0
62	16048.0
63	11724.0
64	9485.0
65	7326.0
66	4125.0
67	2535.5
68	1570.0
69	1001.5
70	736.0
71	621.0
72	433.5
73	200.0
74	112.0
75	58.5
76	42.0
77	29.5
78	15.0
79	8.0
80	8.5
81	6.5
82	4.0
83	5.0
84	5.0
85	3.0
86	2.0
87	2.5
88	2.0
89	1.0
90	1.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3360203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.659692092307456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34041047760604	13.051271169418937
2	9.02306463141644	3.0064295697677514
3	2.9306374730839524	1.464705538072699
4	1.4742740654627284	0.9824380796113354
5	0.8997354499550001	0.7494657780392003
6	0.6092623835881379	0.6090074228402216
7	0.4189291195922395	0.48854610986357094
8	0.3387653482877201	0.45149811152133695
9	0.2711951514084039	0.40662249474516393
>10	2.6765250727233183	10.566249424561844
>50	1.1965677506987429	14.541931517676659
>100	1.8142982430780015	52.10168396234246
>500	0.004524880785254312	0.4821504395925585
>1k	0.0016289570826915523	0.43321171767518996
>5k	0.0	0.0
>10k+	1.809952314101725E-4	0.6647886642712405
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22047	0.6561210736375154	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9760106755454953E-5	0.0	0.0	0.04151534892385966	0.0
2	2.9760106755454953E-5	0.0	0.0	0.1399915421776601	0.0
3	2.9760106755454953E-5	0.0	0.0	0.21165387924479562	0.0
4	2.9760106755454953E-5	0.0	0.0	0.31861170292390073	0.0
5	2.9760106755454953E-5	0.0	0.0	0.5997554314426836	0.0
6	2.9760106755454953E-5	0.0	0.0	0.7982553435015682	0.0
7	2.9760106755454953E-5	0.0	0.0	0.9455976320478257	0.0
8	2.9760106755454953E-5	0.0	0.0	1.2465913517724971	0.0
9	2.9760106755454953E-5	0.0	0.0	1.3538765366259122	0.0
10	2.9760106755454953E-5	0.0	0.0	1.5502039608916485	0.0
11	2.9760106755454953E-5	0.0	0.0	1.936638947111231	0.0
12	2.9760106755454953E-5	0.0	0.0	2.2037954254549503	0.0
13	2.9760106755454953E-5	0.0	0.0	2.294117349457756	0.0
14	2.9760106755454953E-5	0.0	0.0	2.33232932653176	0.0
15	2.9760106755454953E-5	0.0	0.0	2.387802165523928	0.0
16	2.9760106755454953E-5	0.0	0.0	2.513449336245459	0.0
17	2.9760106755454953E-5	0.0	0.0	2.6842723490217706	0.0
18	2.9760106755454953E-5	0.0	0.0	2.887087476560196	0.0
19	2.9760106755454953E-5	0.0	0.0	3.0363344119388027	0.0
20	2.9760106755454953E-5	0.0	0.0	3.1866527111606056	0.0
21	2.9760106755454953E-5	0.0	0.0	3.367088238418929	0.0
22	2.9760106755454953E-5	0.0	0.0	3.573742419728808	0.0
23	2.9760106755454953E-5	0.0	0.0	3.788550870289682	0.0
24	2.9760106755454953E-5	0.0	0.0	3.9531540207541034	0.0
25	2.9760106755454953E-5	0.0	0.0	4.09656797520864	0.0
26	2.9760106755454953E-5	0.0	0.0	4.232631183294581	0.0
27	2.9760106755454953E-5	0.0	0.0	4.381223396324567	0.0
28	2.9760106755454953E-5	0.0	0.0	4.537196115829906	0.0
29	2.9760106755454953E-5	0.0	0.0	4.730845130487652	0.0
30	2.9760106755454953E-5	0.0	0.0	4.93770763254482	0.0
31	2.9760106755454953E-5	0.0	0.0	5.123976140727212	0.0
32	2.9760106755454953E-5	0.0	0.0	5.298638207274977	0.0
33	2.9760106755454953E-5	0.0	0.0	5.471157546136349	0.0
34	2.9760106755454953E-5	0.0	0.0	5.6606401458483315	0.0
35	2.9760106755454953E-5	0.0	0.0	5.871311941570196	0.0
36	2.9760106755454953E-5	0.0	0.0	6.065288317402252	0.0
37	2.9760106755454953E-5	0.0	0.0	6.27090089497569	0.0
38	2.9760106755454953E-5	0.0	0.0	6.497256266957681	0.0
39	2.9760106755454953E-5	0.0	0.0	6.801850959599762	0.0
40	2.9760106755454953E-5	0.0	0.0	7.028206331581752	0.0
41	2.9760106755454953E-5	0.0	0.0	7.249532245522071	0.0
42	2.9760106755454953E-5	0.0	0.0	7.484220447395589	0.0
43	2.9760106755454953E-5	0.0	0.0	7.710218698096514	0.0
44	2.9760106755454953E-5	0.0	0.0	7.944966420183542	0.0
45	2.9760106755454953E-5	0.0	0.0	8.170756350137179	0.0
46	2.9760106755454953E-5	0.0	0.0	8.41249769731174	0.0
47	2.9760106755454953E-5	0.0	0.0	8.6640598797156	0.0
48	2.9760106755454953E-5	0.0	0.0	8.911783008348008	0.0
49	2.9760106755454953E-5	0.0	0.0	9.167958007298964	0.0
50	2.9760106755454953E-5	0.0	0.0	9.42282356155268	0.0
51	2.9760106755454953E-5	0.0	0.0	9.674951185984895	0.0
52	2.9760106755454953E-5	0.0	0.0	9.987164465956372	0.0
53	2.9760106755454953E-5	0.0	0.0	10.248726044230066	0.0
54	2.9760106755454953E-5	0.0	0.0	10.532637462677107	0.0
55	2.9760106755454953E-5	0.0	0.0	10.809674296463637	0.0
56	2.9760106755454953E-5	0.0	0.0	11.086859930783943	0.0
57	2.9760106755454953E-5	0.0	0.0	11.362408759232702	0.0
58	2.9760106755454953E-5	0.0	0.0	11.643135846256907	0.0
59	2.9760106755454953E-5	0.0	0.0	11.926749663636393	0.0
60	2.9760106755454953E-5	0.0	0.0	12.241254471828041	0.0
61	2.9760106755454953E-5	0.0	0.0	12.549747738455087	0.0
62	2.9760106755454953E-5	0.0	0.0	12.902196682759941	0.0
63	2.9760106755454953E-5	0.0	0.0	13.245866395571934	0.0
64	2.9760106755454953E-5	0.0	0.0	13.59167883607032	0.0
65	2.9760106755454953E-5	0.0	0.0	13.898178175544752	0.0
66	2.9760106755454953E-5	0.0	0.0	14.211343778932404	0.0
67	2.9760106755454953E-5	0.0	0.0	14.536294384595216	0.0
68	2.9760106755454953E-5	0.0	0.0	14.886094679398834	0.0
69	2.9760106755454953E-5	0.0	0.0	15.333567644573854	0.0
70	2.9760106755454953E-5	0.0	0.0	15.665214274256645	0.0
71	2.9760106755454953E-5	0.0	0.0	15.996712103405658	0.0
72	2.9760106755454953E-5	0.0	0.0	16.328269452768183	0.0
73	2.9760106755454953E-5	0.0	0.0	16.66155288832252	0.0
74	5.9520213510909906E-5	0.0	0.0	17.028703325364567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12795	0.0	72.55959	1
ACGGGAT	2030	0.0	69.359604	5
GTTAGCG	270	0.0	68.14815	1
TTAGCGG	970	0.0	68.04124	2
CGGGATT	1995	0.0	65.96491	6
GGGCGAT	2325	0.0	65.72043	7
TACGGGA	1455	0.0	65.70447	4
GCGGGAT	3295	0.0	65.55387	5
CGTAAGG	480	0.0	64.166664	2
TTAGGGA	6590	0.0	63.186646	4
CGAGGGA	2465	0.0	63.12373	4
CGGGATA	1105	0.0	62.986423	6
TAGGGGT	5720	0.0	62.93706	5
CGGGATC	1375	0.0	62.254547	6
GTTTGCG	270	0.0	62.22222	1
AGGGATC	5715	0.0	62.22222	6
CTACGGG	990	0.0	61.818184	3
TAGCGGG	2310	0.0	61.64502	3
CGGGAAT	1085	0.0	61.56682	6
TAAGGGA	5190	0.0	61.27168	4
>>END_MODULE