FastQCFastQC Report
Thu 26 May 2016
SRR1547277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547277_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2583216
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111350.43105183616081666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78150.072.1689151
CCGACGG700.068.571432
TTAGCGG5900.068.474582
CGATAGG3800.068.421052
TACGGGA9000.067.555564
TTGCGAG3100.067.096771
TAACGCG303.796924E-666.666671
TAATGCG1450.066.20691
CGAGGGA19450.064.9871444
TAGGGAC33250.063.1578945
ACGGGAT16250.063.0153855
TAGCGGG15700.062.1656073
GGGCGAT18750.061.2266657
ATTGCGG6250.060.82
AGGGATT53750.060.651166
GGCGATA4950.060.6060648
ATTAGCG2250.060.4444431
CGGGATC9700.060.206196
GTAGGGA42950.060.162984
TTGCGGG20050.059.8503763
Produced by FastQC (version 0.11.5)