##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547277_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2583216
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.197715560758375	33.0	31.0	34.0	31.0	34.0
2	32.64417687100111	34.0	31.0	34.0	31.0	34.0
3	32.75921796706121	34.0	31.0	34.0	31.0	34.0
4	36.26245075905383	37.0	37.0	37.0	35.0	37.0
5	36.24458814129364	37.0	37.0	37.0	35.0	37.0
6	36.300657784714865	37.0	37.0	37.0	35.0	37.0
7	36.45034561569764	37.0	37.0	37.0	35.0	37.0
8	36.44352040247505	37.0	37.0	37.0	35.0	37.0
9	38.254346132882425	39.0	39.0	39.0	37.0	39.0
10-11	37.88865332980285	39.0	38.0	39.0	35.0	39.0
12-13	37.177159981975954	39.0	35.0	39.0	34.5	39.0
14-15	38.35212909025029	40.0	36.0	41.0	34.5	41.0
16-17	38.5053899867452	40.0	36.0	41.0	35.0	41.0
18-19	38.39903263993409	40.0	36.0	41.0	35.0	41.0
20-21	38.250406082960154	40.0	35.0	41.0	34.0	41.0
22-23	38.07121316994011	40.0	35.0	41.0	34.0	41.0
24-25	38.04950573238939	40.0	35.0	41.0	34.0	41.0
26-27	38.06390619290063	40.0	35.0	41.0	34.0	41.0
28-29	38.007619571882486	40.0	35.0	41.0	34.0	41.0
30-31	37.879285936599956	40.0	35.5	41.0	34.0	41.0
32-33	37.59133924534379	40.0	35.0	41.0	33.5	41.0
34-35	37.309368244854475	40.0	35.0	41.0	33.0	41.0
36-37	37.145384474236764	40.0	35.0	41.0	33.0	41.0
38-39	37.0583814903593	40.0	35.0	41.0	33.0	41.0
40-41	36.94136069147915	40.0	35.0	41.0	32.5	41.0
42-43	36.892229492229845	39.5	35.0	41.0	33.0	41.0
44-45	36.762054934624125	39.0	35.0	41.0	32.0	41.0
46-47	36.63801768802919	39.0	35.0	41.0	32.0	41.0
48-49	36.55783701401664	39.0	35.0	41.0	32.0	41.0
50-51	36.333784321558866	38.5	35.0	40.5	31.5	41.0
52-53	36.16775678069507	38.0	35.0	40.0	31.0	41.0
54-55	36.00111450223288	38.0	35.0	40.0	31.0	41.0
56-57	35.74484073341138	37.0	35.0	40.0	30.5	41.0
58-59	35.51340247969972	37.0	35.0	40.0	30.0	41.0
60-61	35.16738805427033	36.0	35.0	40.0	29.5	41.0
62-63	34.796492627794194	36.0	34.0	39.0	28.5	41.0
64-65	34.47051988683873	35.0	34.0	39.0	28.5	40.0
66-67	34.167321664158166	35.0	34.0	38.5	28.0	40.0
68-69	33.70490543570495	35.0	34.0	37.5	26.5	40.0
70-71	33.42034541439818	35.0	34.0	37.0	26.0	39.0
72-73	33.15963299236301	35.0	34.0	36.5	26.0	39.0
74-75	32.851687199212144	35.0	33.0	36.0	25.5	38.5
76-77	30.92858011099343	33.5	30.0	34.5	22.5	36.0
78-79	31.94049123263405	34.5	32.5	35.0	24.0	37.0
80-81	32.07683252194164	35.0	33.0	35.0	24.0	37.0
82-83	31.881220927711816	35.0	33.0	35.0	23.5	36.0
84-85	31.66833861357316	35.0	33.0	35.0	22.5	36.0
86	31.252750447504198	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	14.0
11	23.0
12	37.0
13	86.0
14	245.0
15	645.0
16	1580.0
17	3764.0
18	8728.0
19	15864.0
20	19487.0
21	19278.0
22	16489.0
23	13822.0
24	12562.0
25	13462.0
26	15772.0
27	19135.0
28	23997.0
29	31525.0
30	41716.0
31	55214.0
32	71907.0
33	99780.0
34	281891.0
35	260501.0
36	219419.0
37	417639.0
38	624064.0
39	293797.0
40	763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40542022037646	24.613505026292806	29.49714619296257	13.483928560368161
2	31.08532155266923	25.358738874333387	30.819451412502865	12.736488160494517
3	28.904396690017403	24.26854742305715	33.03397780131433	13.793078085611112
4	26.657623675294673	26.766015695164473	31.467945382809642	15.108415246731207
5	24.281709311184198	31.650276244804925	29.745092938414754	14.322921505596126
6	23.241300766176735	40.63562628909081	25.495777356597358	10.6272955881351
7	89.04710252646314	3.174763550550941	6.142962880378567	1.6351710426073547
8	89.64894921679023	2.683476720491047	5.986994506073049	1.6805795566456696
9	85.24269747477562	5.167202432936309	7.179771261868926	2.4103288304191364
10-11	39.76233501186118	28.384637599023854	19.78889879901642	12.064128590098544
12-13	29.544683835962616	24.668494620658898	28.554890493090785	17.231931050287702
14-15	17.582579234566527	30.88948039962589	34.26099482195836	17.266945543849218
16-17	22.621840372620795	25.686295687236377	32.16163495425857	19.530228985884264
18-19	24.180982155576615	27.249715083833486	30.207752661798317	18.361550098791586
20-21	26.7977784281299	27.24117921226874	29.094237570532233	16.86680478906913
22-23	21.960261937058302	24.24261076115973	31.757352075862027	22.03977522591994
24-25	20.30606422382023	26.724962217638787	33.242438882385365	19.726534676155612
26-27	19.01414748127915	32.28359533232993	29.590982713021287	19.11127447336963
28-29	17.661666697635813	27.948359719047883	35.19310812568519	19.196865457631105
30-31	22.54610144873677	27.573207195991355	30.847130089005333	19.033561266266545
32-33	25.071654867421074	27.73192408222928	28.705981226502157	18.49043982384748
34-35	20.15321212008597	28.485171197453095	30.427052944856335	20.9345637376046
36-37	22.684881945605788	28.739079503998116	29.772849037788557	18.803189512607542
38-39	20.689133235470823	30.007111290732176	28.327712432874367	20.976043040922633
40-41	21.12635954562065	26.943604406290454	28.870620962397258	23.059415085691633
42-43	21.04065242705217	26.688631535264566	28.473325498138752	23.797390539544505
44-45	19.79451195718825	29.86428544883587	28.50104288607689	21.84015970789899
46-47	21.16681299589349	29.124858316145456	28.489119763891214	21.21920892406984
48-49	21.422327827018727	25.892685706499186	31.096993050523068	21.587993415959023
50-51	19.633336894785415	29.65510820620498	28.91289385014648	21.79866104886312
52-53	21.675965153514067	26.266289772129003	30.316764064638807	21.74098100971812
54-55	21.400281664405917	26.73049795293928	31.25338725062093	20.615833132033867
56-57	21.55268858663	25.89400189531189	29.322364060922506	23.2309454571356
58-59	22.91196322723303	28.106012040804952	28.018930666270265	20.96309406569176
60-61	23.827953218004225	28.422671584567453	27.304491765303407	20.444883432124918
62-63	22.242448947358643	26.049815423874733	30.383580002601406	21.324155626165215
64-65	19.77980161163449	29.63542344116791	29.87270518609361	20.712069761103987
66-67	19.752200358003357	28.97678707471617	27.1223738162043	24.148638751076177
68-69	19.120952332286574	30.566588314720878	26.950824089042495	23.361635263950053
70-71	19.578153743240982	29.130587608624285	29.05643585360264	22.234822794532086
72-73	21.75956249883866	29.7848108714099	27.52443078704994	20.9311958427015
74-75	20.91702745724709	30.878370217589236	26.3385833782386	21.866018946925074
76-77	23.50448046156419	28.04595124836638	27.434310564815327	21.015257725254102
78-79	19.58802515933627	29.08291447559941	29.582756532941882	21.74630383212244
80-81	18.11509374361261	29.08465649020446	29.90806808257614	22.89218168360679
82-83	19.414423726084078	27.641397389920165	30.679877331202654	22.264301552793107
84-85	17.651621080080023	27.80427575549238	29.25456098135038	25.289542183077216
86	18.7948278425033	27.744176251618136	30.53968386693176	22.9213120389468
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	789.0
1	1040.5
2	3875.5
3	4341.5
4	2501.0
5	3159.0
6	3839.0
7	4138.0
8	4303.5
9	4568.0
10	4600.0
11	4511.5
12	4609.5
13	4914.0
14	5099.0
15	5345.0
16	5612.5
17	5406.0
18	5509.0
19	6061.5
20	6536.5
21	6790.0
22	7874.0
23	9609.0
24	10783.5
25	13571.0
26	17919.0
27	21478.5
28	24333.5
29	25713.0
30	30745.0
31	39375.5
32	48673.0
33	53849.5
34	60651.0
35	68190.0
36	68405.0
37	69808.5
38	76231.0
39	84800.5
40	93962.5
41	107050.5
42	120609.0
43	126691.0
44	134367.0
45	143292.5
46	144433.0
47	144169.0
48	138675.0
49	123898.0
50	114460.0
51	107084.5
52	92209.5
53	81940.5
54	75693.0
55	70726.0
56	66907.5
57	64578.0
58	57781.0
59	42620.5
60	30178.5
61	24125.5
62	19404.0
63	14065.5
64	11474.0
65	9674.0
66	6902.0
67	4996.0
68	3249.5
69	2195.0
70	1504.5
71	1056.0
72	924.0
73	615.0
74	373.5
75	227.5
76	104.5
77	67.5
78	51.0
79	30.0
80	27.0
81	22.5
82	16.5
83	10.5
84	8.5
85	6.5
86	5.0
87	6.5
88	5.5
89	4.5
90	5.0
91	3.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2583216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.272625503952987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00339507188907	12.855926615456958
2	8.866378490307866	2.885585134981679
3	2.7675085518883518	1.3510389073159919
4	1.3073148830814987	0.8509378203251726
5	0.7976959908146419	0.6490304062265695
6	0.5090033858387308	0.49696928867986523
7	0.3696558246336303	0.42106895597325894
8	0.28648208930247304	0.37294526022473284
9	0.22258825289101064	0.3259885752787137
>10	2.704930383398104	10.690292321027762
>50	1.2784866480999497	15.278710063067328
>100	1.8834480819765895	52.98635346975714
>500	0.001915289771156486	0.2072981331223877
>1k	9.57644885578243E-4	0.19405224602191565
>5k	0.0	0.0
>10k+	2.3941122139456075E-4	0.43380280254045983
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11135	0.43105183616081666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1613430700336325E-4	0.0	0.0	0.03495642640801234	0.0
2	1.1613430700336325E-4	0.0	0.0	0.10227561303429523	0.0
3	1.1613430700336325E-4	0.0	0.0	0.15689744876154374	0.0
4	1.1613430700336325E-4	0.0	0.0	0.2729543328935714	0.0
5	1.1613430700336325E-4	0.0	0.0	0.5242302618131817	0.0
6	1.1613430700336325E-4	0.0	0.0	0.7145743909916941	0.0
7	1.1613430700336325E-4	0.0	0.0	0.8381800050789403	0.0
8	1.1613430700336325E-4	0.0	0.0	1.1079212888120853	0.0
9	1.1613430700336325E-4	0.0	0.0	1.1926606214888729	0.0
10	1.1613430700336325E-4	0.0	0.0	1.3719719915020656	0.0
11	1.1613430700336325E-4	0.0	0.0	1.6757019157515283	0.0
12	1.1613430700336325E-4	0.0	0.0	1.9125771906027216	0.0
13	1.1613430700336325E-4	0.0	0.0	1.9933679568413947	0.0
14	1.1613430700336325E-4	0.0	0.0	2.0244532396826282	0.0
15	1.1613430700336325E-4	0.0	0.0	2.0759781605564536	0.0
16	1.1613430700336325E-4	0.0	0.0	2.1856089463676285	0.0
17	1.1613430700336325E-4	0.0	0.0	2.331667193142192	0.0
18	1.1613430700336325E-4	0.0	0.0	2.519146676081288	0.0
19	1.1613430700336325E-4	0.0	0.0	2.643100693089544	0.0
20	1.1613430700336325E-4	0.0	0.0	2.7809521155025365	0.0
21	1.1613430700336325E-4	0.0	0.0	2.9481468061517115	0.0
22	1.1613430700336325E-4	0.0	0.0	3.130826071068002	0.0
23	1.1613430700336325E-4	0.0	0.0	3.3182281311357626	0.0
24	1.1613430700336325E-4	0.0	0.0	3.456969916569114	0.0
25	1.1613430700336325E-4	0.0	0.0	3.582627236746753	0.0
26	1.1613430700336325E-4	0.0	0.0	3.7039488761295996	0.0
27	1.1613430700336325E-4	0.0	0.0	3.8323159968039837	0.0
28	1.1613430700336325E-4	0.0	0.0	3.970128707781308	0.0
29	1.54845742671151E-4	0.0	0.0	4.131942508872661	0.0
30	1.54845742671151E-4	0.0	0.0	4.305795566456696	0.0
31	1.54845742671151E-4	0.0	0.0	4.471015973886814	0.0
32	1.54845742671151E-4	0.0	0.0	4.621332478584834	0.0
33	1.54845742671151E-4	0.0	0.0	4.78082359353612	0.0
34	1.54845742671151E-4	0.0	0.0	4.948482821413308	0.0
35	1.54845742671151E-4	0.0	0.0	5.136155861530743	0.0
36	1.54845742671151E-4	0.0	0.0	5.302847303516237	0.0
37	1.54845742671151E-4	0.0	0.0	5.493075298387746	0.0
38	1.54845742671151E-4	0.0	0.0	5.687135725390366	0.0
39	1.54845742671151E-4	0.0	0.0	5.961406247096642	0.0
40	1.54845742671151E-4	0.0	0.0	6.174473989012146	0.0
41	1.54845742671151E-4	0.0	0.0	6.385993273500938	0.0
42	1.54845742671151E-4	0.0	0.0	6.592983320016599	0.0
43	1.54845742671151E-4	0.0	0.0	6.783985543601465	0.0
44	1.54845742671151E-4	0.0	0.0	6.972665081046261	0.0
45	1.54845742671151E-4	0.0	0.0	7.168312676911261	0.0
46	1.54845742671151E-4	0.0	0.0	7.382270781847124	0.0
47	1.54845742671151E-4	0.0	0.0	7.618681519470304	0.0
48	1.54845742671151E-4	0.0	0.0	7.83136214702913	0.0
49	1.54845742671151E-4	0.0	0.0	8.055656205288292	0.0
50	1.54845742671151E-4	0.0	0.0	8.27654365720869	0.0
51	1.54845742671151E-4	0.0	0.0	8.499637660962149	0.0
52	1.54845742671151E-4	0.0	0.0	8.759623662907012	0.0
53	1.54845742671151E-4	0.0	0.0	8.991234182507386	0.0
54	1.9355717833893874E-4	0.0	0.0	9.233800038401744	0.0
55	1.9355717833893874E-4	0.0	0.0	9.470249487460592	0.0
56	1.9355717833893874E-4	0.0	0.0	9.706389245034098	0.0
57	1.9355717833893874E-4	0.0	0.0	9.946167877560374	0.0
58	1.9355717833893874E-4	0.0	0.0	10.180681754835833	0.0
59	1.9355717833893874E-4	0.0	0.0	10.423596013651201	0.0
60	1.9355717833893874E-4	0.0	0.0	10.681956135298016	0.0
61	1.9355717833893874E-4	0.0	0.0	10.952974896408199	0.0
62	1.9355717833893874E-4	0.0	0.0	11.236458739803409	0.0
63	1.9355717833893874E-4	0.0	0.0	11.55102786603985	0.0
64	2.322686140067265E-4	0.0	0.0	11.839544196071873	0.0
65	2.7098004967451427E-4	0.0	0.0	12.102588401434492	0.0
66	2.7098004967451427E-4	0.0	0.0	12.356961245207524	0.0
67	2.7098004967451427E-4	0.0	0.0	12.63595456206527	0.0
68	2.7098004967451427E-4	0.0	0.0	12.92032876848084	0.0
69	2.7098004967451427E-4	0.0	0.0	13.302178369907898	0.0
70	2.7098004967451427E-4	0.0	0.0	13.57478429988046	0.0
71	2.7098004967451427E-4	0.0	0.0	13.85160977634081	0.0
72	3.09691485342302E-4	0.0	0.0	14.121970443044638	0.0
73	3.09691485342302E-4	0.0	0.0	14.412267499117378	0.0
74	3.09691485342302E-4	0.0	0.0	14.709997150838335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7815	0.0	72.168915	1
CCGACGG	70	0.0	68.57143	2
TTAGCGG	590	0.0	68.47458	2
CGATAGG	380	0.0	68.42105	2
TACGGGA	900	0.0	67.55556	4
TTGCGAG	310	0.0	67.09677	1
TAACGCG	30	3.796924E-6	66.66667	1
TAATGCG	145	0.0	66.2069	1
CGAGGGA	1945	0.0	64.987144	4
TAGGGAC	3325	0.0	63.157894	5
ACGGGAT	1625	0.0	63.015385	5
TAGCGGG	1570	0.0	62.165607	3
GGGCGAT	1875	0.0	61.226665	7
ATTGCGG	625	0.0	60.8	2
AGGGATT	5375	0.0	60.65116	6
GGCGATA	495	0.0	60.606064	8
ATTAGCG	225	0.0	60.444443	1
CGGGATC	970	0.0	60.20619	6
GTAGGGA	4295	0.0	60.16298	4
TTGCGGG	2005	0.0	59.850376	3
>>END_MODULE