FastQCFastQC Report
Thu 26 May 2016
SRR1547276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547276_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3223060
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133310.41361315023611106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94550.071.665791
TACGGGA17400.070.3448264
CTAGCGG5350.068.0373842
TAGCGGG24150.067.080753
TAGGGAC41650.066.6506655
CGTAAGG6550.066.564892
TAGGGAT55400.065.415175
ACGGGAT21650.065.21945
CGGGATT21050.064.038016
CTACGGG6200.063.8709683
GTAGGGA56600.062.897534
CGGGATC11150.062.7802666
TTAGGGA54250.062.672814
AGGGATT66950.062.554156
GCGTAAG2750.062.5454561
ACGGGAC13200.061.8181845
GAGGGAT56500.061.5221255
TAAGGGA51850.061.0993234
GCGGGAT25100.060.876495
CGTTAGG3900.060.512822
Produced by FastQC (version 0.11.5)