##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547276_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3223060
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06848957202162	33.0	31.0	34.0	31.0	34.0
2	32.50129318101432	34.0	31.0	34.0	31.0	34.0
3	32.629225332448044	34.0	31.0	34.0	31.0	34.0
4	36.15272194746607	37.0	35.0	37.0	35.0	37.0
5	36.12419160673397	37.0	35.0	37.0	35.0	37.0
6	36.18138352993739	37.0	36.0	37.0	35.0	37.0
7	36.38008569496069	37.0	37.0	37.0	35.0	37.0
8	36.38111266932666	37.0	37.0	37.0	35.0	37.0
9	38.169370101704594	39.0	39.0	39.0	37.0	39.0
10-11	37.75415552301229	39.0	37.5	39.0	35.0	39.0
12-13	37.140241106277884	39.0	36.0	39.0	34.0	39.0
14-15	38.31945573461245	40.0	37.0	41.0	33.5	41.0
16-17	38.46241211767699	40.0	37.0	41.0	34.0	41.0
18-19	38.3177710002296	40.0	36.0	41.0	34.0	41.0
20-21	38.184316301899436	40.0	35.5	41.0	34.0	41.0
22-23	38.00600857570135	40.0	35.0	41.0	34.0	41.0
24-25	37.99217994700688	40.0	35.0	41.0	34.0	41.0
26-27	37.99736601242297	40.0	35.0	41.0	34.0	41.0
28-29	37.92547765167264	40.0	35.0	41.0	34.0	41.0
30-31	37.796065850465084	40.0	35.5	41.0	34.0	41.0
32-33	37.5334883619914	40.0	35.0	41.0	33.0	41.0
34-35	37.24974279101227	40.0	35.0	41.0	33.0	41.0
36-37	37.08224513350667	40.0	35.0	41.0	32.0	41.0
38-39	36.98279026763386	40.0	35.0	41.0	32.0	41.0
40-41	36.858642408146295	40.0	35.0	41.0	31.5	41.0
42-43	36.82110851178693	39.5	35.0	41.0	32.0	41.0
44-45	36.707275539394246	39.0	35.0	41.0	31.0	41.0
46-47	36.57695001023872	39.0	35.0	41.0	31.0	41.0
48-49	36.47385683170651	39.0	35.0	41.0	31.0	41.0
50-51	36.25175950804515	38.5	35.0	40.0	31.0	41.0
52-53	36.06305095158018	38.0	35.0	40.0	30.0	41.0
54-55	35.86296780078558	38.0	35.0	40.0	30.0	41.0
56-57	35.57598338225165	37.0	35.0	40.0	29.5	41.0
58-59	35.32457478297022	37.0	35.0	40.0	29.0	41.0
60-61	34.97184368271146	36.0	34.0	39.5	28.5	41.0
62-63	34.62158057870471	36.0	34.0	39.0	28.0	41.0
64-65	34.28580370827723	35.0	34.0	39.0	27.5	40.0
66-67	33.995270333161656	35.0	34.0	38.0	26.5	40.0
68-69	33.52580498036028	35.0	33.5	37.5	26.0	40.0
70-71	33.23269982563154	35.0	33.0	37.0	26.0	39.0
72-73	32.99059201504161	35.0	33.0	36.5	25.5	39.0
74-75	32.69654489832644	35.0	33.0	36.0	25.0	38.5
76-77	30.748649109852128	33.0	30.0	34.5	21.5	36.0
78-79	31.82224780177843	34.5	32.0	35.0	23.5	37.0
80-81	31.937414599790262	35.0	33.0	35.0	24.0	36.5
82-83	31.758875106265474	35.0	32.5	35.0	23.0	36.0
84-85	31.544893517340665	34.5	32.0	35.0	21.5	36.0
86	31.135622358876347	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	12.0
10	16.0
11	23.0
12	72.0
13	108.0
14	368.0
15	1039.0
16	2448.0
17	5610.0
18	11713.0
19	18651.0
20	23089.0
21	24297.0
22	21474.0
23	18707.0
24	18521.0
25	20200.0
26	23856.0
27	29147.0
28	36467.0
29	46164.0
30	58822.0
31	75420.0
32	93899.0
33	125948.0
34	321901.0
35	290325.0
36	290190.0
37	538347.0
38	783387.0
39	342149.0
40	686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.255465303159106	24.078515448052475	30.545258232859457	13.120761015928961
2	30.635389971021326	25.123361029580586	31.196875019391513	13.044373980006577
3	29.35852885146414	25.163074841920412	32.080817608111545	13.397578698503906
4	26.611884358342692	27.08624723089238	31.725441040501885	14.576427370263042
5	24.106253063858567	32.36669500412651	29.338237575471755	14.188814356543162
6	23.70297171011399	40.903147940156245	24.777044175410946	10.616836174318815
7	89.1074630940783	3.3263420476193435	5.935787729673043	1.630407128629315
8	89.88473686496683	2.586641266374191	5.745130404025988	1.7834914646329887
9	85.062611307267	5.607559275967558	6.913181883055233	2.416647533710201
10-11	38.068543557985265	29.13315296643562	20.22059781698138	12.57770565859773
12-13	29.804673198761428	23.54303053619852	28.253662668395872	18.39863359664418
14-15	18.14814182795232	30.062518228019336	33.45272815274925	18.336611791279093
16-17	24.63785967372621	26.460739173332176	30.151734686912434	18.749666466029176
18-19	24.339649277394773	27.755269836739	29.45432291052602	18.450757975340203
20-21	26.943572257419966	27.41205562415841	29.063731360880652	16.58064075754097
22-23	23.339698919660197	23.92138216477509	31.823081171309255	20.91583774425546
24-25	20.93411230321496	27.10289290301763	32.29472923246852	19.668265561298888
26-27	20.14216303761022	31.308554603389325	28.850471291257378	19.698811067743076
28-29	18.75523570768152	27.87701749269328	33.10050076635247	20.26724603327273
30-31	22.698072639044884	27.178457738918915	30.32811986124987	19.795349760786333
32-33	24.95451527430454	27.321737727501194	28.955697380749974	18.768049617444294
34-35	21.520232325802187	27.65897935502287	30.04805991821437	20.772728400960577
36-37	22.9116895124509	28.97364616234262	28.931807040514297	19.182857284692187
38-39	22.03575173903061	29.220321681879952	28.039564885543548	20.704361693545888
40-41	21.906495690430834	27.201339720638153	28.737085254385587	22.155079334545434
42-43	21.32827189068773	26.881209161480086	28.654989357939346	23.135529589892833
44-45	20.944009729884023	29.234050870911492	27.868919598145865	21.95301980105862
46-47	21.628948887082462	28.555332510099095	28.34204141405993	21.473677188758508
48-49	21.864811700681962	26.566911568509425	30.31960311008793	21.24867362072068
50-51	20.37920485501356	28.742251152631347	28.840046415518174	22.038497576836917
52-53	22.232940125222616	26.544200232077593	29.806255545971837	21.416604096727955
54-55	22.02211562924674	26.120069126854606	30.701445210452178	21.156370033446475
56-57	22.397256023778645	26.126801859102837	29.037157235670453	22.438784881448065
58-59	23.209325920088364	27.526620664834038	28.300217805439555	20.963835609638046
60-61	23.84770994024312	28.167626417131547	27.40695177874442	20.577711863880907
62-63	22.912915055878575	26.425989587534826	29.630242688625096	21.030852667961504
64-65	21.276783553517465	28.782864731032	29.232189285958064	20.708162429492468
66-67	21.34508820810038	28.377628713086324	27.41528237141102	22.862000707402284
68-69	20.80811402828368	29.952064187449196	27.22305821176149	22.01676357250563
70-71	20.619085589470878	28.487260553635362	28.90658256439533	21.987071292498435
72-73	21.989584432185563	29.366238915812925	27.568428760246473	21.07574789175504
74-75	21.242918220572996	30.185863744391977	26.66231779737268	21.908900237662348
76-77	23.06570464093129	28.031327372124625	27.1597643233449	21.74320366359919
78-79	21.01572729021489	28.545062766439344	28.323953013595776	22.11525692974999
80-81	19.63564128499004	28.626041711913526	28.910460866381637	22.8278561367148
82-83	20.558661644524147	28.147071416604096	29.55675662258847	21.737510316283284
84-85	19.588062276221976	27.62143739179537	28.850098974266693	23.94040135771596
86	20.3666701829938	27.591264202341875	29.3175429560728	22.724522658591525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	602.0
1	1123.5
2	4726.5
3	5356.0
4	3157.0
5	3834.5
6	4483.5
7	4708.0
8	4879.5
9	5154.0
10	5205.0
11	5241.0
12	5251.0
13	5283.5
14	5394.0
15	5479.5
16	5535.5
17	5722.0
18	6058.0
19	6871.0
20	8038.5
21	8513.0
22	8733.5
23	10678.5
24	12776.5
25	15882.5
26	20957.5
27	27512.5
28	33817.5
29	35910.0
30	41026.5
31	49732.5
32	54502.5
33	60337.0
34	66618.0
35	74272.0
36	80299.0
37	83587.5
38	90812.0
39	101521.5
40	118483.5
41	134146.0
42	144020.5
43	148421.0
44	155242.5
45	163569.5
46	169349.5
47	174740.5
48	172442.0
49	158145.5
50	147264.0
51	136902.5
52	120824.0
53	115057.5
54	111492.5
55	102603.0
56	92016.5
57	86804.0
58	78524.0
59	60537.0
60	42858.0
61	33664.0
62	29128.0
63	21501.5
64	17189.0
65	13827.0
66	8895.5
67	6249.0
68	4681.5
69	3522.5
70	2396.0
71	1938.0
72	1536.5
73	906.5
74	540.5
75	334.0
76	165.5
77	92.0
78	81.5
79	45.0
80	37.5
81	22.5
82	17.0
83	13.5
84	8.5
85	6.0
86	3.0
87	3.5
88	2.5
89	0.5
90	1.0
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3223060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.28447190134405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35367297572145	12.922326578415808
2	8.627790107458322	2.809980111511984
3	2.6165791483196856	1.27828828855364
4	1.193290259587408	0.7772840680959477
5	0.6790844504459157	0.552926582596309
6	0.4580362051619651	0.4475326627654968
7	0.3369083648390346	0.384046236038432
8	0.2728054036675679	0.35539935444474646
9	0.2259276814262486	0.33112016819294005
>10	2.8871139915736617	11.72950989611672
>50	1.4658758279187212	17.508777469394047
>100	1.8780974803860657	49.878525438468316
>500	0.0034690345156838756	0.3601326437334729
>1k	0.0011563448385612917	0.24576895277357963
>5k	0.0	0.0
>10k+	1.9272413976021528E-4	0.41838154889855583
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13331	0.41361315023611106	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2410566356195666E-4	0.0	0.0	0.029785359254869595	0.0
2	1.2410566356195666E-4	0.0	0.0	0.09729884023257401	0.0
3	1.2410566356195666E-4	0.0	0.0	0.14306280367104554	0.0
4	1.2410566356195666E-4	0.0	0.0	0.22863365869701463	0.0
5	1.2410566356195666E-4	0.0	0.0	0.43607627534082516	0.0
6	1.2410566356195666E-4	0.0	0.0	0.5914565661203949	0.0
7	1.2410566356195666E-4	0.0	0.0	0.7033067954056084	0.0
8	1.2410566356195666E-4	0.0	0.0	0.9343605145420811	0.0
9	1.2410566356195666E-4	0.0	0.0	1.014967143025572	0.0
10	1.2410566356195666E-4	0.0	0.0	1.1821995246753085	0.0
11	1.2410566356195666E-4	0.0	0.0	1.4581794940212096	0.0
12	1.2410566356195666E-4	0.0	0.0	1.6689419371653025	0.0
13	1.2410566356195666E-4	0.0	0.0	1.742164278666857	0.0
14	1.2410566356195666E-4	0.0	0.0	1.7739353285387178	0.0
15	1.2410566356195666E-4	0.0	0.0	1.824694544935558	0.0
16	1.2410566356195666E-4	0.0	0.0	1.9340006081177514	0.0
17	1.2410566356195666E-4	0.0	0.0	2.0780562570972925	0.0
18	1.2410566356195666E-4	0.0	0.0	2.238121536676326	0.0
19	1.2410566356195666E-4	0.0	0.0	2.3585350567473147	0.0
20	1.2410566356195666E-4	0.0	0.0	2.491886592244637	0.0
21	1.2410566356195666E-4	0.0	0.0	2.6468014867858494	0.0
22	1.2410566356195666E-4	0.0	0.0	2.826165197048767	0.0
23	1.2410566356195666E-4	0.0	0.0	2.998982333558792	0.0
24	1.2410566356195666E-4	0.0	0.0	3.1301930463596706	0.0
25	1.2410566356195666E-4	0.0	0.0	3.2564085062021806	0.0
26	1.2410566356195666E-4	0.0	0.0	3.3724162752167195	0.0
27	1.2410566356195666E-4	0.0	0.0	3.5000899766060822	0.0
28	1.2410566356195666E-4	0.0	0.0	3.6289116553833933	0.0
29	1.2410566356195666E-4	0.0	0.0	3.788604617971741	0.0
30	1.2410566356195666E-4	0.0	0.0	3.9622284412949185	0.0
31	1.551320794524458E-4	0.0	0.0	4.1220765359627185	0.0
32	1.8615849534293498E-4	0.0	0.0	4.271561807723096	0.0
33	1.8615849534293498E-4	0.0	0.0	4.421946845544296	0.0
34	1.8615849534293498E-4	0.0	0.0	4.576551475926604	0.0
35	1.8615849534293498E-4	0.0	0.0	4.755853133357741	0.0
36	2.1718491123342412E-4	0.0	0.0	4.925319416951592	0.0
37	2.1718491123342412E-4	0.0	0.0	5.1024181988545045	0.0
38	2.1718491123342412E-4	0.0	0.0	5.298877464273082	0.0
39	2.1718491123342412E-4	0.0	0.0	5.57336816565624	0.0
40	2.1718491123342412E-4	0.0	0.0	5.783075710660056	0.0
41	2.1718491123342412E-4	0.0	0.0	5.989339323500028	0.0
42	2.1718491123342412E-4	0.0	0.0	6.195044460853971	0.0
43	2.1718491123342412E-4	0.0	0.0	6.378131341023748	0.0
44	2.1718491123342412E-4	0.0	0.0	6.568199164768885	0.0
45	2.482113271239133E-4	0.0	0.0	6.75566076957922	0.0
46	2.482113271239133E-4	0.0	0.0	6.975576005411007	0.0
47	2.482113271239133E-4	0.0	0.0	7.202906554640621	0.0
48	3.102641589048916E-4	0.0	0.0	7.403088989966057	0.0
49	3.102641589048916E-4	0.0	0.0	7.607273832941367	0.0
50	3.102641589048916E-4	0.0	0.0	7.821418155417523	0.0
51	3.412905747953808E-4	0.0	0.0	8.042884712043834	0.0
52	3.412905747953808E-4	0.0	0.0	8.294633050579263	0.0
53	3.412905747953808E-4	0.0	0.0	8.517278611009413	0.0
54	3.412905747953808E-4	0.0	0.0	8.750659311337673	0.0
55	3.412905747953808E-4	0.0	0.0	8.979292970034688	0.0
56	3.412905747953808E-4	0.0	0.0	9.216148628942681	0.0
57	3.7231699068586996E-4	0.0	0.0	9.452321706701085	0.0
58	4.0334340657635913E-4	0.0	0.0	9.672454127444105	0.0
59	4.0334340657635913E-4	0.0	0.0	9.913560405329097	0.0
60	4.0334340657635913E-4	0.0	0.0	10.153208441667235	0.0
61	4.0334340657635913E-4	0.0	0.0	10.407438893473904	0.0
62	4.0334340657635913E-4	0.0	0.0	10.691175466792426	0.0
63	4.0334340657635913E-4	0.0	0.0	10.976091043914789	0.0
64	4.0334340657635913E-4	0.0	0.0	11.245214175348892	0.0
65	4.3436982246684825E-4	0.0	0.0	11.492742921323215	3.1026415890489166E-5
66	4.3436982246684825E-4	0.0	0.0	11.734531780357797	3.1026415890489166E-5
67	4.3436982246684825E-4	0.0	0.0	11.988203756678436	3.1026415890489166E-5
68	4.3436982246684825E-4	0.0	0.0	12.272436752651208	3.1026415890489166E-5
69	4.3436982246684825E-4	0.0	0.0	12.647204830192425	3.1026415890489166E-5
70	4.3436982246684825E-4	0.0	0.0	12.925170490155319	3.1026415890489166E-5
71	4.3436982246684825E-4	0.0	0.0	13.196837787692441	3.1026415890489166E-5
72	4.3436982246684825E-4	0.0	0.0	13.472228255136423	3.1026415890489166E-5
73	4.3436982246684825E-4	0.0	0.0	13.749759545276849	3.1026415890489166E-5
74	4.3436982246684825E-4	0.0	0.0	14.038336239474289	3.1026415890489166E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9455	0.0	71.66579	1
TACGGGA	1740	0.0	70.344826	4
CTAGCGG	535	0.0	68.037384	2
TAGCGGG	2415	0.0	67.08075	3
TAGGGAC	4165	0.0	66.650665	5
CGTAAGG	655	0.0	66.56489	2
TAGGGAT	5540	0.0	65.41517	5
ACGGGAT	2165	0.0	65.2194	5
CGGGATT	2105	0.0	64.03801	6
CTACGGG	620	0.0	63.870968	3
GTAGGGA	5660	0.0	62.89753	4
CGGGATC	1115	0.0	62.780266	6
TTAGGGA	5425	0.0	62.67281	4
AGGGATT	6695	0.0	62.55415	6
GCGTAAG	275	0.0	62.545456	1
ACGGGAC	1320	0.0	61.818184	5
GAGGGAT	5650	0.0	61.522125	5
TAAGGGA	5185	0.0	61.099323	4
GCGGGAT	2510	0.0	60.87649	5
CGTTAGG	390	0.0	60.51282	2
>>END_MODULE