FastQCFastQC Report
Thu 26 May 2016
SRR1547275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547275_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1475376
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138860.9411838067041893No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC16580.11237813276073352No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16040.10871804882280857No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC14910.10105898428603961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67800.073.510331
ACGGGTC1900.071.578955
GTAGCGT403.7143764E-970.000019
GGGCGAT10350.067.632857
GCGTAAG1500.066.6666641
TTTACGG2050.066.341462
GTTAGCG1700.065.8823551
ACGGGAT4750.065.684215
CGTATGG1950.065.641022
GACGGGT1950.065.641024
CGAACGG1100.065.4545442
CGGGATA2450.065.306126
CGATAGG2650.064.905662
ATTGCGG3400.064.705882
TAGGGTA6050.064.132235
ACGTGCG503.0013325E-1064.01
ACGTACT251.2356426E-464.080
TAGGGAC15550.063.7942125
CGTAGGG7000.063.428573
CGGGATC3850.063.3766256
Produced by FastQC (version 0.11.5)