##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547275_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1475376 Sequences flagged as poor quality 0 Sequence length 86 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01981935452386 33.0 31.0 34.0 31.0 34.0 2 32.441596582837185 34.0 31.0 34.0 31.0 34.0 3 32.53606267148171 34.0 31.0 34.0 31.0 34.0 4 36.07802553383002 37.0 35.0 37.0 35.0 37.0 5 36.04105123033044 37.0 35.0 37.0 35.0 37.0 6 36.09263672446888 37.0 35.0 37.0 35.0 37.0 7 36.33324318682153 37.0 37.0 37.0 35.0 37.0 8 36.32534147227554 37.0 37.0 37.0 35.0 37.0 9 38.111544446974875 39.0 39.0 39.0 37.0 39.0 10-11 37.64409750463611 39.0 37.5 39.0 35.0 39.0 12-13 36.76316579638004 39.0 35.0 39.0 33.5 39.0 14-15 37.82782185693681 40.0 35.0 41.0 33.0 41.0 16-17 38.01892636182234 40.0 35.0 41.0 34.0 41.0 18-19 37.880865962303844 39.5 36.0 41.0 34.0 41.0 20-21 37.64915960406026 39.0 35.0 41.0 34.0 41.0 22-23 37.44187752816909 39.0 35.0 41.0 33.0 41.0 24-25 37.405677264643046 39.0 35.0 41.0 33.0 41.0 26-27 37.36318436791706 39.0 35.0 41.0 33.5 41.0 28-29 37.30406587879971 39.0 35.0 41.0 33.0 41.0 30-31 37.15030914153409 39.0 35.0 41.0 33.0 41.0 32-33 36.77129558837883 39.0 35.0 41.0 32.5 41.0 34-35 36.42268886033119 39.0 35.0 41.0 31.0 41.0 36-37 36.21185887529687 38.0 35.0 41.0 30.5 41.0 38-39 36.13261941362744 38.0 35.0 41.0 30.5 41.0 40-41 36.01227754823177 38.0 35.0 41.0 30.0 41.0 42-43 35.96835789656332 38.0 35.0 41.0 30.0 41.0 44-45 35.850537761221545 38.0 35.0 41.0 30.0 41.0 46-47 35.70650532474434 38.0 35.0 40.0 30.0 41.0 48-49 35.62050148572296 37.5 35.0 40.0 29.0 41.0 50-51 35.3826336472872 37.0 35.0 40.0 29.0 41.0 52-53 35.19870527919663 37.0 35.0 40.0 28.0 41.0 54-55 35.04713984774051 36.0 35.0 40.0 28.0 41.0 56-57 34.76608810228714 36.0 34.0 40.0 27.5 41.0 58-59 34.49898093774061 36.0 34.0 39.5 26.0 41.0 60-61 34.13497067866089 35.0 34.0 39.0 26.0 41.0 62-63 33.65730430751212 35.0 33.5 39.0 24.5 40.0 64-65 33.22221996291115 35.0 33.0 38.0 23.0 40.0 66-67 32.86397738610361 35.0 33.0 37.0 21.5 40.0 68-69 32.38166643621693 35.0 33.0 36.5 19.5 39.0 70-71 32.029109189792976 35.0 32.5 36.0 18.5 39.0 72-73 31.73176397067595 35.0 32.0 36.0 17.5 38.5 74-75 31.43363895034215 35.0 32.0 35.0 16.0 37.5 76-77 29.53036039626509 33.0 29.0 34.5 11.5 35.5 78-79 30.441358338484562 34.0 30.5 35.0 12.0 36.0 80-81 30.62031305917949 34.5 31.0 35.0 11.0 36.0 82-83 30.412756138096324 34.5 31.0 35.0 10.0 36.0 84-85 30.175342082289532 34.0 31.0 35.0 10.0 35.5 86 29.752145215863617 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 3.0 10 6.0 11 9.0 12 16.0 13 80.0 14 188.0 15 634.0 16 1637.0 17 3923.0 18 8527.0 19 13014.0 20 14395.0 21 13955.0 22 12250.0 23 10968.0 24 10839.0 25 12236.0 26 14627.0 27 18090.0 28 22411.0 29 28243.0 30 36108.0 31 44983.0 32 54223.0 33 68380.0 34 180858.0 35 171852.0 36 119889.0 37 201921.0 38 284690.0 39 126189.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.331454490245196 24.266356508442595 30.919169079610892 14.483019921701315 2 31.353431260912473 25.44978364837167 30.296412575506178 12.900372515209682 3 28.326880740909438 24.80784559325894 33.77274674388088 13.092526921950743 4 26.110089902506207 26.620739391178926 33.46841754237564 13.800753163939225 5 24.23307685634035 31.505799199661645 30.51696652243225 13.744157421565756 6 22.49928153907885 40.00471744151999 27.15633167409528 10.339669345305873 7 86.24615013393196 3.5571949116699746 8.450252681350381 1.7464022730476843 8 86.54871707280043 3.5329299107481753 7.825191679951415 2.0931613364999837 9 82.50391764540022 5.178137640845453 9.234798451377818 3.083146262376506 10-11 42.99178650052597 24.837092375096248 20.034791131209943 12.136329993167843 12-13 29.975240209953256 27.28043563132381 26.695432215245468 16.048891943477457 14-15 16.27513935430697 33.01802388001431 35.09339314181605 15.613443623862663 16-17 20.124497077355198 25.41874071423149 33.39341971131427 21.063342497099043 18-19 23.775159688106626 27.921323106787693 29.99167669800783 18.311840507097852 20-21 25.78535912201364 28.920864918502133 28.881790133498175 16.411985825986054 22-23 21.095097114227155 26.58129181984796 30.491888169524245 21.831722896400645 24-25 19.40691728752535 27.964600210387047 34.005433191267855 18.623049310819752 26-27 18.066580993590787 34.75974937914132 29.17080120592988 18.002868421338018 28-29 16.61728942317077 29.193405613213173 34.949158722928935 19.24014624068712 30-31 22.07179729099565 30.40574063831864 29.451644868833437 18.070817201852275 32-33 26.025738523603476 30.148213065686306 26.55756905358363 17.26847935712659 34-35 20.179703343418897 30.67594294606934 29.12620240535294 20.01815130515882 36-37 23.04649797746473 31.106375595102538 28.710918437062823 17.13620799036991 38-39 20.739797854919694 32.169291082408826 27.32767782585592 19.763233236815562 40-41 20.87078141436488 28.356500309073756 27.778207046881608 22.994511229679755 42-43 21.385158766307708 27.98249395408357 27.336150242378892 23.29619703722983 44-45 19.39298863476158 32.87812733838696 27.073742557829327 20.655141469022134 46-47 21.415320569129495 31.369088286646928 26.760330925811456 20.455260218412118 48-49 21.739034659639305 27.563380453525067 30.479959007059893 20.21762587977573 50-51 19.338799058680635 32.03037734109813 27.272742677120952 21.35808092310028 52-53 21.99391883831647 27.380139028966177 29.784712507184608 20.841229625532744 54-55 21.513634490462092 28.118425404778176 30.78733827851341 19.580601826246326 56-57 22.27479639088612 26.244496318226673 28.771818167029966 22.70888912385724 58-59 23.662747665679802 28.87535109694071 27.476216232336707 19.985685005042782 60-61 23.770855700512953 31.27612893255685 25.56209400180022 19.390921365129973 62-63 22.660596349676286 28.432616499116158 29.23810608278839 19.66868106841917 64-65 19.2117805901682 32.98281251694483 28.770767587381112 19.034639305505852 66-67 18.361116081595473 31.622854106343063 25.16466988754053 24.851359924520935 68-69 18.399682521608053 34.191351899448 24.26286587283513 23.146099706108817 70-71 18.975637396839858 31.80762056587609 27.77430973528104 21.442432302003013 72-73 21.78481959852946 32.14465329516001 26.10568424591426 19.964842860396264 74-75 21.319378924423333 34.07395809610567 23.750352452527355 20.856310526943638 76-77 25.15677359534112 30.257270011170036 25.16585602585375 19.420100367635097 78-79 19.566469835486007 31.357193013848672 28.543910162561954 20.53242698810337 80-81 17.25705853965362 31.024803168819336 29.137148767500626 22.580989524026418 82-83 18.981025853748466 29.843341629523596 29.243833436357917 21.93179908037002 84-85 16.794972942490595 29.831954701716718 28.32145839433473 25.051613961457956 86 18.797919987853945 28.620636366594006 30.562988688985044 22.018454956567005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 575.0 1 904.0 2 4965.5 3 5487.5 4 2651.0 5 4056.0 6 6137.0 7 7187.0 8 7102.0 9 6489.5 10 5248.5 11 4288.5 12 4024.5 13 3918.5 14 3830.0 15 3923.0 16 4020.0 17 4299.5 18 4559.0 19 4828.5 20 5113.0 21 5112.0 22 5223.5 23 5493.5 24 5903.5 25 7502.0 26 9211.5 27 10514.0 28 13002.0 29 14550.0 30 17171.0 31 21285.0 32 23469.0 33 24604.5 34 28110.0 35 32364.5 36 33558.0 37 36221.5 38 41286.5 39 48553.5 40 56701.5 41 65594.5 42 75391.5 43 79578.0 44 81057.5 45 83142.5 46 87635.0 47 89567.0 48 83642.5 49 71226.5 50 62780.0 51 56620.5 52 48170.5 53 43330.5 54 39845.0 55 38316.0 56 35542.5 57 33362.0 58 30430.5 59 23834.5 60 16086.0 61 10711.0 62 7850.0 63 5423.5 64 4567.0 65 3757.0 66 2655.0 67 2007.0 68 1665.5 69 1217.0 70 622.0 71 490.0 72 401.5 73 278.0 74 204.0 75 109.0 76 45.0 77 27.5 78 14.0 79 10.0 80 10.5 81 7.5 82 6.0 83 5.0 84 1.5 85 1.0 86 1.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 86 1475376.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.310121399980467 #Duplication Level Percentage of deduplicated Percentage of total 1 78.27728507739192 16.68098447860101 2 8.845774599590554 3.7700906118827655 3 3.0635425629932653 1.9585339179418138 4 1.594945717052386 1.3595394742706097 5 0.9689020381454954 1.0323710028784505 6 0.703079017755633 0.8989619533290937 7 0.5074862218715196 0.7570215097829655 8 0.40712204974876826 0.6940656243804111 9 0.31029598195736374 0.5951200540893813 >10 3.082571118259484 14.93291265168384 >50 1.0418485871771985 16.102100643189775 >100 1.1920131892869668 39.376913421991524 >500 0.003529514154012034 0.4910814927306552 >1k 0.0012834596923680126 0.4008242426047203 >5k 0.0 0.0 >10k+ 3.2086492309200314E-4 0.9494789206429796 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13886 0.9411838067041893 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC 1658 0.11237813276073352 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1604 0.10871804882280857 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC 1491 0.10105898428603961 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04175206862521825 0.0 2 0.0 0.0 0.0 0.20320243788702 0.0 3 0.0 0.0 0.0 0.3287975404235937 0.0 4 0.0 0.0 0.0 0.5725320189565236 0.0 5 0.0 0.0 0.0 1.1842404919152814 0.0 6 0.0 0.0 0.0 1.652121221980024 0.0 7 0.0 0.0 0.0 1.9826810250403966 0.0 8 0.0 0.0 0.0 2.544707247508432 0.0 9 0.0 0.0 0.0 2.7087332313932175 0.0 10 0.0 0.0 0.0 3.1328285128672286 0.0 11 0.0 0.0 0.0 3.701293771892724 0.0 12 0.0 0.0 0.0 4.13691153983798 0.0 13 0.0 0.0 0.0 4.286093849974515 0.0 14 0.0 0.0 0.0 4.342892930344536 0.0 15 0.0 0.0 0.0 4.441918534665062 0.0 16 0.0 0.0 0.0 4.651017774452072 0.0 17 0.0 0.0 0.0 4.90810478142521 0.0 18 0.0 0.0 0.0 5.199488130483348 0.0 19 0.0 0.0 0.0 5.400386071076119 0.0 20 0.0 0.0 0.0 5.593624947132121 0.0 21 0.0 0.0 0.0 5.8481363394822745 0.0 22 0.0 0.0 0.0 6.105630022448515 0.0 23 0.0 0.0 0.0 6.406705816008936 0.0 24 0.0 0.0 0.0 6.616550628449968 0.0 25 6.777933218379585E-5 0.0 0.0 6.815279630412857 0.0 26 6.777933218379585E-5 0.0 0.0 6.994623743371181 0.0 27 6.777933218379585E-5 0.0 0.0 7.199927340555899 0.0 28 6.777933218379585E-5 0.0 0.0 7.404553144418779 0.0 29 6.777933218379585E-5 0.0 0.0 7.621650345403477 0.0 30 6.777933218379585E-5 0.0 0.0 7.857115745409984 0.0 31 6.777933218379585E-5 0.0 0.0 8.086074329526847 0.0 32 6.777933218379585E-5 0.0 0.0 8.293953541334547 0.0 33 6.777933218379585E-5 0.0 0.0 8.512338549630739 0.0 34 6.777933218379585E-5 0.0 0.0 8.730181323269457 0.0 35 6.777933218379585E-5 0.0 0.0 8.980625955688584 0.0 36 6.777933218379585E-5 0.0 0.0 9.212905727082452 0.0 37 6.777933218379585E-5 0.0 0.0 9.46355369749813 0.0 38 6.777933218379585E-5 0.0 0.0 9.714811681903461 0.0 39 6.777933218379585E-5 0.0 0.0 10.060892952033923 0.0 40 6.777933218379585E-5 0.0 0.0 10.322250056934639 0.0 41 6.777933218379585E-5 0.0 0.0 10.590113977725 0.0 42 6.777933218379585E-5 0.0 0.0 10.864823611065924 0.0 43 6.777933218379585E-5 0.0 0.0 11.111811497543677 0.0 44 6.777933218379585E-5 0.0 0.0 11.361713895305332 0.0 45 6.777933218379585E-5 0.0 0.0 11.630187830085347 0.0 46 6.777933218379585E-5 0.0 0.0 11.887749292383772 0.0 47 6.777933218379585E-5 0.0 0.0 12.170728004251119 0.0 48 6.777933218379585E-5 0.0 0.0 12.43804969038401 0.0 49 6.777933218379585E-5 0.0 0.0 12.72157063690883 0.0 50 6.777933218379585E-5 0.0 0.0 13.013767337953173 0.0 51 6.777933218379585E-5 0.0 0.0 13.305421804340046 0.0 52 6.777933218379585E-5 0.0 0.0 13.624798017590093 0.0 53 6.777933218379585E-5 0.0 0.0 13.927907191116027 0.0 54 6.777933218379585E-5 0.0 0.0 14.22579735606381 0.0 55 6.777933218379585E-5 0.0 0.0 14.519756319744932 0.0 56 6.777933218379585E-5 0.0 0.0 14.818663174675473 0.0 57 6.777933218379585E-5 0.0 0.0 15.123466831506002 0.0 58 6.777933218379585E-5 0.0 0.0 15.399735389487155 0.0 59 6.777933218379585E-5 0.0 0.0 15.692203207860233 0.0 60 6.777933218379585E-5 0.0 0.0 15.995719057379272 0.0 61 6.777933218379585E-5 0.0 0.0 16.31963648588563 0.0 62 6.777933218379585E-5 0.0 0.0 16.66009207144484 0.0 63 6.777933218379585E-5 0.0 0.0 17.019458090683322 0.0 64 6.777933218379585E-5 0.0 0.0 17.34683226513106 0.0 65 6.777933218379585E-5 0.0 0.0 17.65699048920411 0.0 66 6.777933218379585E-5 0.0 0.0 17.952847274186375 0.0 67 6.777933218379585E-5 0.0 0.0 18.248500721172096 0.0 68 1.355586643675917E-4 0.0 0.0 18.58034833154396 0.0 69 1.355586643675917E-4 0.0 0.0 18.993870037197297 0.0 70 1.355586643675917E-4 0.0 0.0 19.312161781132403 0.0 71 1.355586643675917E-4 0.0 0.0 19.635062519656007 0.0 72 1.355586643675917E-4 0.0 0.0 19.943051805099174 0.0 73 1.355586643675917E-4 0.0 0.0 20.251718883864182 0.0 74 2.711173287351834E-4 0.0 0.0 20.58993775146132 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 6780 0.0 73.51033 1 ACGGGTC 190 0.0 71.57895 5 GTAGCGT 40 3.7143764E-9 70.00001 9 GGGCGAT 1035 0.0 67.63285 7 GCGTAAG 150 0.0 66.666664 1 TTTACGG 205 0.0 66.34146 2 GTTAGCG 170 0.0 65.882355 1 ACGGGAT 475 0.0 65.68421 5 CGTATGG 195 0.0 65.64102 2 GACGGGT 195 0.0 65.64102 4 CGAACGG 110 0.0 65.454544 2 CGGGATA 245 0.0 65.30612 6 CGATAGG 265 0.0 64.90566 2 ATTGCGG 340 0.0 64.70588 2 TAGGGTA 605 0.0 64.13223 5 ACGTGCG 50 3.0013325E-10 64.0 1 ACGTACT 25 1.2356426E-4 64.0 80 TAGGGAC 1555 0.0 63.794212 5 CGTAGGG 700 0.0 63.42857 3 CGGGATC 385 0.0 63.376625 6 >>END_MODULE