FastQCFastQC Report
Thu 26 May 2016
SRR1547274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547274_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5027359
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590901.1753686179960492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT341500.076.3221051
CGTAAGG10450.065.4545442
TAGCGCG3600.064.444441
TAGGGAT80500.063.155285
TAACGGG14350.062.1602823
GGGCGAT36800.060.543487
CGAGGGA32400.060.493834
TACGGGA21100.060.4739344
ATAGGGA79600.060.3015024
AGGGATC72150.059.9861376
GTACGGG18600.059.784953
AGGGATT91650.059.399896
TTGCGGG35900.059.3871843
TAAGGGA71600.059.3854754
AAGGGAT91000.059.2527475
GCGGGAT37550.059.22775
TACGGGT6950.058.7050364
GTAGGGA73250.058.5938574
GGCGATT13000.058.4615448
TAGGGAC49250.058.395945
Produced by FastQC (version 0.11.5)