##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547274_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5027359
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.003005355296885	33.0	31.0	34.0	31.0	34.0
2	32.435000166091186	34.0	31.0	34.0	31.0	34.0
3	32.509827724656226	34.0	31.0	34.0	31.0	34.0
4	36.07861065820046	37.0	35.0	37.0	35.0	37.0
5	36.05030832291865	37.0	35.0	37.0	35.0	37.0
6	36.10207526456734	37.0	36.0	37.0	35.0	37.0
7	36.34174842099003	37.0	37.0	37.0	35.0	37.0
8	36.343595911889324	37.0	37.0	37.0	35.0	37.0
9	38.14253109833612	39.0	39.0	39.0	37.0	39.0
10-11	37.67210835748949	39.0	37.5	39.0	35.0	39.0
12-13	37.16078949205736	39.0	37.0	39.0	33.5	39.0
14-15	38.32532757258831	40.0	37.0	41.0	33.0	41.0
16-17	38.466151909978976	40.0	37.0	41.0	34.0	41.0
18-19	38.23579706561636	40.0	37.0	41.0	34.0	41.0
20-21	37.996189550020205	40.0	35.0	41.0	34.0	41.0
22-23	37.83277492218082	40.0	35.0	41.0	33.5	41.0
24-25	37.78811031796218	40.0	35.0	41.0	34.0	41.0
26-27	37.761424139394066	40.0	35.0	41.0	34.0	41.0
28-29	37.65110468538252	40.0	35.0	41.0	33.0	41.0
30-31	37.42589429161514	40.0	35.0	41.0	33.0	41.0
32-33	36.96200559777012	40.0	35.0	41.0	31.5	41.0
34-35	36.492310773907334	40.0	35.0	41.0	30.0	41.0
36-37	36.25942418275679	40.0	35.0	41.0	30.0	41.0
38-39	36.14531098733947	39.5	35.0	41.0	29.0	41.0
40-41	36.01718635967712	39.0	35.0	41.0	28.5	41.0
42-43	35.9603731303056	39.0	35.0	41.0	28.0	41.0
44-45	35.84238503755152	39.0	35.0	41.0	27.5	41.0
46-47	35.723673204957116	39.0	35.0	41.0	27.0	41.0
48-49	35.62361609743804	39.0	35.0	41.0	27.0	41.0
50-51	35.40125352098388	38.0	35.0	40.0	26.5	41.0
52-53	35.20693926970404	38.0	35.0	40.0	26.0	41.0
54-55	35.00020149744627	37.5	34.5	40.0	25.5	41.0
56-57	34.704584355324535	37.0	34.0	40.0	25.0	41.0
58-59	34.42906285387616	36.5	34.0	40.0	25.0	41.0
60-61	34.068916502680636	36.0	34.0	39.5	23.5	41.0
62-63	33.70437957583694	35.5	33.5	39.0	22.5	40.5
64-65	33.34465004388984	35.0	33.0	39.0	22.0	40.0
66-67	33.01108812798131	35.0	33.0	38.0	20.5	40.0
68-69	32.490043082262474	35.0	32.5	37.0	18.5	40.0
70-71	32.17781940776459	35.0	32.0	37.0	18.5	39.0
72-73	31.927144450993055	35.0	32.0	36.0	18.0	39.0
74-75	31.633807034667704	35.0	32.0	36.0	17.5	38.0
76-77	29.721371797796817	33.0	29.0	34.5	13.0	36.0
78-79	30.80405924860349	34.0	31.0	35.0	15.0	36.5
80-81	30.886264338791	34.5	31.0	35.0	15.0	36.0
82-83	30.691029823014432	34.0	31.0	35.0	11.5	36.0
84-85	30.467130256661598	34.0	31.0	35.0	10.5	36.0
86	30.046768492164574	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	9.0
10	12.0
11	38.0
12	76.0
13	233.0
14	773.0
15	2561.0
16	6591.0
17	15885.0
18	36717.0
19	59772.0
20	69455.0
21	66039.0
22	54607.0
23	44393.0
24	39516.0
25	40210.0
26	45737.0
27	53710.0
28	65455.0
29	80486.0
30	100561.0
31	126626.0
32	153265.0
33	194378.0
34	434191.0
35	415060.0
36	430892.0
37	802781.0
38	1188403.0
39	497893.0
40	1029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.363596472820024	22.946640572117488	30.32777249446479	16.3619904605977
2	33.51767796968548	24.033394074304223	30.09255953274871	12.356368423261598
3	27.623549462053536	23.76042769175625	35.49903637277545	13.116986473414768
4	25.65609497949122	26.196478111071837	34.615809215136615	13.531617694300326
5	23.091607342940897	31.179790422764714	32.34055892964875	13.388043304645638
6	22.16205367470276	39.104289150625604	28.83134067012123	9.902316504550402
7	86.14914908603104	2.815951675621335	9.605659750974617	1.4292394873729926
8	87.14036534888398	2.11094930757879	9.276242257614783	1.4724430859224495
9	82.39952627214409	4.780482157729336	10.682567129182539	2.1374244409440424
10-11	34.690510464838496	26.656978345886976	24.615916627398203	14.036594561876326
12-13	28.51429547800346	22.040439125194762	30.89682873254128	18.5484366642605
14-15	18.709226852508447	27.913055343769962	35.39061960763096	17.98709819609063
16-17	24.77147544068367	25.843479648061734	32.03079390192743	17.35425100932716
18-19	23.606470116814812	26.58251579009973	31.80384571700569	18.007168376079765
20-21	25.283951275411205	26.879699659403677	31.852807806245785	15.983541258939335
22-23	22.309506840470313	22.658019847001178	34.96468026253944	20.06779304998907
24-25	20.790438876555264	26.65343771948651	33.85291362721461	18.703209776743616
26-27	20.010019177066926	29.684442666616807	31.288187296749648	19.017350859566623
28-29	18.64363575388191	27.155669607044175	34.40174055602554	19.798954083048375
30-31	21.869683466010684	26.268494054234043	32.68578989485334	19.176032584901932
32-33	23.42011978854106	26.47296522886072	31.122732631586487	18.984182351011732
34-35	20.44227396531658	27.709310196466973	31.470778195867847	20.3776376423486
36-37	21.98328187821876	28.546409755102033	30.43543339554625	19.034874971132954
38-39	21.220177433121446	28.790364881441725	29.5585415722251	20.43091611321173
40-41	21.32937989906828	27.087074943325113	29.822318239059513	21.761226918547095
42-43	21.208749961958155	26.896060138136146	29.941993400511084	21.95319649939461
44-45	20.80907689305657	28.376777946432707	29.190346263316385	21.623798897194334
46-47	20.85988886013511	28.063919843400882	29.74821372414423	21.32797757231978
48-49	21.265041943493593	26.423575479690232	31.11844608670278	21.192936490113397
50-51	19.798228055724685	28.09510918158023	30.390210844302146	21.716451918392938
52-53	21.252053016305382	26.44450097954015	30.79012260711837	21.513323397036096
54-55	20.85205174327117	26.6163307613401	31.329421670503336	21.202195824885393
56-57	21.076284784913906	26.727383105125373	30.225193784649157	21.97113832531156
58-59	21.97814001347427	27.780092887736878	29.424256354081734	20.817510744707114
60-61	22.5091345177458	28.227862780438002	28.7863866495311	20.476616052285106
62-63	21.353209508212963	27.33571443773958	30.062235460009916	21.248840594037546
64-65	20.639564431344567	29.048383057585504	29.60437080383557	20.707681707234354
66-67	20.52143083475837	28.774392280320544	28.233820182724166	22.47035670219692
68-69	19.83780151765569	29.57670220089713	28.353823548308366	22.23167273313881
70-71	20.238240396200073	28.732740192216234	29.409158963980893	21.619860447602807
72-73	20.973338088646543	29.321011290421072	28.374172204531245	21.331478416401136
74-75	20.205380200618254	29.863085170563707	27.852934314020544	22.07860031479749
76-77	21.82091034278634	28.243427612788345	28.32165954331091	21.614002501114403
78-79	19.71972759454815	28.761303897334567	29.212296157883294	22.30667235023399
80-81	19.198241064543033	28.699412554384917	29.26614152679369	22.83620485427836
82-83	19.470282508171785	28.61943020182167	29.741013124385983	22.169274165620557
84-85	19.0868306798858	28.38040808305116	28.942651598980696	23.59010963808234
86	19.506902132909147	28.385997498885594	29.11695783014501	22.990142538060244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1761.5
2	15345.5
3	18545.0
4	9157.5
5	10924.5
6	12939.5
7	13729.0
8	14511.0
9	15674.0
10	16179.5
11	16598.5
12	16997.0
13	17289.5
14	17478.0
15	17714.5
16	17972.5
17	18213.5
18	18368.0
19	18427.0
20	19591.5
21	20632.0
22	21713.5
23	24015.0
24	26364.0
25	28896.5
26	33973.5
27	40502.5
28	48782.5
29	54207.0
30	58049.0
31	65989.5
32	78995.0
33	92043.5
34	103767.0
35	116751.5
36	122154.0
37	126293.0
38	138425.0
39	157049.5
40	179284.5
41	199124.0
42	220492.5
43	233625.0
44	235761.5
45	244402.0
46	255728.5
47	263270.0
48	262454.0
49	245934.0
50	232949.0
51	221151.0
52	199684.5
53	178593.0
54	165693.0
55	153949.5
56	133441.5
57	123200.0
58	113576.5
59	89476.0
60	66007.5
61	49451.0
62	36019.0
63	25534.5
64	20917.0
65	16929.5
66	10854.0
67	7464.5
68	5209.5
69	3480.0
70	2230.0
71	1756.0
72	1406.0
73	944.5
74	566.5
75	243.0
76	168.5
77	111.0
78	57.5
79	44.0
80	62.0
81	54.0
82	23.0
83	16.0
84	11.0
85	7.0
86	6.0
87	7.0
88	6.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	5027359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.530359972854768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54722582583923	14.740387293947702
2	8.214545352759718	3.0443696479995768
3	2.6992389438415727	1.5005360784639796
4	1.3135374051730873	0.9736128382266757
5	0.797762500827139	0.7391413156585867
6	0.5630361613907475	0.6259957648982943
7	0.412523318883457	0.5350943917275051
8	0.31305061638636555	0.4640752489090737
9	0.25889144125066604	0.4317616440239421
>10	3.050100790219449	13.676706378351597
>50	1.350468060068906	18.189175878756522
>100	1.4750330648653116	43.05436178979048
>500	0.0028392733537350332	0.3494296512156344
>1k	0.0016380423194614485	0.47962598987194544
>5k	0.0	0.0
>10k+	1.0920282129742989E-4	1.1957260881585206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59090	1.1753686179960492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.945579776578517E-5	0.0	0.0	0.033496712687516444	0.0
2	9.945579776578517E-5	0.0	0.0	0.10418989373943655	0.0
3	1.1934695731894221E-4	0.0	0.0	0.16151621557163512	0.0
4	1.1934695731894221E-4	0.0	0.0	0.2588834415843388	0.0
5	1.1934695731894221E-4	0.0	0.0	0.5071648951268449	0.0
6	1.1934695731894221E-4	0.0	0.0	0.76759984715633	0.0
7	1.1934695731894221E-4	0.0	0.0	0.9652582996360515	0.0
8	1.1934695731894221E-4	0.0	0.0	1.2958692625690746	0.0
9	1.1934695731894221E-4	0.0	0.0	1.445271761972837	0.0
10	1.1934695731894221E-4	0.0	0.0	1.675671063077055	0.0
11	1.1934695731894221E-4	1.9891159553157036E-5	0.0	1.990448663005765	0.0
12	1.591292764252563E-4	1.9891159553157036E-5	0.0	2.204895254148351	0.0
13	1.591292764252563E-4	1.9891159553157036E-5	0.0	2.2885972535480357	0.0
14	1.591292764252563E-4	1.9891159553157036E-5	0.0	2.3328550835538104	0.0
15	1.7902043597841331E-4	1.9891159553157036E-5	0.0	2.387933704356502	0.0
16	1.7902043597841331E-4	1.9891159553157036E-5	0.0	2.4972356261011	0.0
17	1.7902043597841331E-4	1.9891159553157036E-5	0.0	2.636433960654093	0.0
18	1.7902043597841331E-4	1.9891159553157036E-5	0.0	2.8010333059564676	0.0
19	1.7902043597841331E-4	1.9891159553157036E-5	0.0	2.9207581953069197	0.0
20	1.7902043597841331E-4	1.9891159553157036E-5	0.0	3.0552622162053673	0.0
21	1.7902043597841331E-4	1.9891159553157036E-5	0.0	3.224814460236478	0.0
22	1.7902043597841331E-4	1.9891159553157036E-5	0.0	3.4165055648502523	0.0
23	1.7902043597841331E-4	1.9891159553157036E-5	0.0	3.608355798740452	0.0
24	1.7902043597841331E-4	1.9891159553157036E-5	0.0	3.7571018898789603	0.0
25	1.9891159553157034E-4	1.9891159553157036E-5	0.0	3.8966980476230164	0.0
26	1.9891159553157034E-4	1.9891159553157036E-5	0.0	4.028297959226704	0.0
27	1.9891159553157034E-4	1.9891159553157036E-5	0.0	4.176288186302192	0.0
28	2.188027550847274E-4	1.9891159553157036E-5	0.0	4.328336209926524	0.0
29	2.188027550847274E-4	1.9891159553157036E-5	0.0	4.514815830737371	0.0
30	2.188027550847274E-4	1.9891159553157036E-5	0.0	4.714960678161237	0.0
31	2.3869391463788442E-4	1.9891159553157036E-5	0.0	4.892469386013611	0.0
32	2.3869391463788442E-4	1.9891159553157036E-5	0.0	5.060728704673766	0.0
33	2.3869391463788442E-4	1.9891159553157036E-5	0.0	5.227456404048328	0.0
34	2.3869391463788442E-4	1.9891159553157036E-5	0.0	5.408804105694461	0.0
35	2.3869391463788442E-4	1.9891159553157036E-5	0.0	5.606880272524799	0.0
36	2.3869391463788442E-4	1.9891159553157036E-5	0.0	5.793081417101901	0.0
37	2.5858507419104147E-4	1.9891159553157036E-5	0.0	5.992370944664982	0.0
38	2.5858507419104147E-4	1.9891159553157036E-5	0.0	6.219169945890079	0.0
39	2.5858507419104147E-4	1.9891159553157036E-5	0.0	6.5822830635329606	0.0
40	2.5858507419104147E-4	5.9673478659471105E-5	0.0	6.820280787586484	0.0
41	2.5858507419104147E-4	5.9673478659471105E-5	0.0	7.068462785331224	0.0
42	2.5858507419104147E-4	5.9673478659471105E-5	0.0	7.30293977414384	0.0
43	2.5858507419104147E-4	5.9673478659471105E-5	0.0	7.5225779579297996	0.0
44	2.5858507419104147E-4	5.9673478659471105E-5	0.0	7.7355128209463455	0.0
45	2.5858507419104147E-4	5.9673478659471105E-5	0.0	7.947671928740319	0.0
46	2.5858507419104147E-4	5.9673478659471105E-5	0.0	8.177951882887218	0.0
47	2.5858507419104147E-4	5.9673478659471105E-5	0.0	8.418615022320864	0.0
48	2.5858507419104147E-4	5.9673478659471105E-5	0.0	8.660392066689488	0.0
49	2.5858507419104147E-4	5.9673478659471105E-5	0.0	8.896858171457419	0.0
50	2.5858507419104147E-4	5.9673478659471105E-5	0.0	9.140644222940912	0.0
51	2.5858507419104147E-4	5.9673478659471105E-5	0.0	9.396086493922555	0.0
52	2.5858507419104147E-4	5.9673478659471105E-5	0.0	9.693141070689402	0.0
53	2.5858507419104147E-4	5.9673478659471105E-5	0.0	9.940408075094696	0.0
54	2.5858507419104147E-4	5.9673478659471105E-5	0.0	10.194895570417788	0.0
55	2.5858507419104147E-4	5.9673478659471105E-5	0.0	10.444867772522313	0.0
56	2.5858507419104147E-4	5.9673478659471105E-5	0.0	10.704805445562968	1.9891159553157036E-5
57	2.5858507419104147E-4	5.9673478659471105E-5	0.0	10.965140941794688	1.9891159553157036E-5
58	2.7847623374419847E-4	5.9673478659471105E-5	0.0	11.222751349167625	1.9891159553157036E-5
59	2.7847623374419847E-4	5.9673478659471105E-5	0.0	11.48551356686483	1.9891159553157036E-5
60	2.7847623374419847E-4	5.9673478659471105E-5	0.0	11.77156833239878	1.9891159553157036E-5
61	2.983673932973555E-4	5.9673478659471105E-5	0.0	12.062575996661467	1.9891159553157036E-5
62	3.182585528505126E-4	5.9673478659471105E-5	0.0	12.391993490021301	1.9891159553157036E-5
63	3.381497124036696E-4	5.9673478659471105E-5	0.0	12.71357386651719	1.9891159553157036E-5
64	3.5804087195682663E-4	5.9673478659471105E-5	0.0	13.015700688970094	1.9891159553157036E-5
65	3.5804087195682663E-4	5.9673478659471105E-5	0.0	13.304261740607743	1.9891159553157036E-5
66	3.5804087195682663E-4	5.9673478659471105E-5	0.0	13.584190028999322	1.9891159553157036E-5
67	3.779320315099837E-4	5.9673478659471105E-5	0.0	13.87022490337372	1.9891159553157036E-5
68	3.978231910631407E-4	5.9673478659471105E-5	0.0	14.193993307420456	1.9891159553157036E-5
69	4.1771435061629773E-4	5.9673478659471105E-5	0.0	14.633568042385674	1.9891159553157036E-5
70	4.376055101694548E-4	5.9673478659471105E-5	0.0	14.961036202109298	1.9891159553157036E-5
71	4.5749666972261184E-4	5.9673478659471105E-5	0.0	15.270622209394634	1.9891159553157036E-5
72	4.972789888289258E-4	5.9673478659471105E-5	0.0	15.57547412070632	1.9891159553157036E-5
73	5.171701483820829E-4	5.9673478659471105E-5	0.0	15.8755720448848	1.9891159553157036E-5
74	5.370613079352399E-4	5.9673478659471105E-5	0.0	16.19749057109309	1.9891159553157036E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34150	0.0	76.322105	1
CGTAAGG	1045	0.0	65.454544	2
TAGCGCG	360	0.0	64.44444	1
TAGGGAT	8050	0.0	63.15528	5
TAACGGG	1435	0.0	62.160282	3
GGGCGAT	3680	0.0	60.54348	7
CGAGGGA	3240	0.0	60.49383	4
TACGGGA	2110	0.0	60.473934	4
ATAGGGA	7960	0.0	60.301502	4
AGGGATC	7215	0.0	59.986137	6
GTACGGG	1860	0.0	59.78495	3
AGGGATT	9165	0.0	59.39989	6
TTGCGGG	3590	0.0	59.387184	3
TAAGGGA	7160	0.0	59.385475	4
AAGGGAT	9100	0.0	59.252747	5
GCGGGAT	3755	0.0	59.2277	5
TACGGGT	695	0.0	58.705036	4
GTAGGGA	7325	0.0	58.593857	4
GGCGATT	1300	0.0	58.461544	8
TAGGGAC	4925	0.0	58.39594	5
>>END_MODULE