FastQCFastQC Report
Thu 26 May 2016
SRR1547273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547273_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2813500
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT857823.048942598187311No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC30600.10876132930513595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT252200.076.27281
GCGATCG351.18210664E-768.571439
GGCGATT8850.067.3446358
GCGGGAT15650.066.198085
TAGCGGG17000.065.4117663
CGAGGGA16800.065.23814
ACGGGAT9700.064.742275
CGGGATC8350.064.6706546
TACGGGA10100.064.554464
CGTAAGG5700.063.859652
TAGGGTA20150.063.1265535
ACGGGTC5150.062.912625
CGGGATT11450.062.1834076
GACCGAT6700.062.0895549
TAAGGGA38000.061.8947374
ATTGCGG7450.061.744972
ATAGGGA36250.061.682764
AGGGATT47150.060.996826
TAGGGAC28400.060.9859165
TAGGGCG7300.060.821925
Produced by FastQC (version 0.11.5)