##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547273_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2813500
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0292379598365	33.0	31.0	34.0	31.0	34.0
2	32.44881322196552	34.0	31.0	34.0	31.0	34.0
3	32.486175937444465	34.0	31.0	34.0	31.0	34.0
4	36.065346010307444	37.0	35.0	37.0	35.0	37.0
5	36.046633374800074	37.0	35.0	37.0	35.0	37.0
6	36.11110360760618	37.0	36.0	37.0	35.0	37.0
7	36.359273147325396	37.0	37.0	37.0	35.0	37.0
8	36.36492660387418	37.0	37.0	37.0	35.0	37.0
9	38.162196196907765	39.0	39.0	39.0	37.0	39.0
10-11	37.687574373556075	39.0	37.5	39.0	35.0	39.0
12-13	37.00536431491025	39.0	35.0	39.0	33.5	39.0
14-15	38.157750133285944	40.0	36.0	41.0	33.0	41.0
16-17	38.335972631953084	40.0	36.0	41.0	34.0	41.0
18-19	38.10026905988982	40.0	36.0	41.0	34.0	41.0
20-21	37.775240980984535	40.0	35.0	41.0	34.0	41.0
22-23	37.57469806291097	39.0	35.0	41.0	33.5	41.0
24-25	37.500516438599604	39.0	35.0	41.0	34.0	41.0
26-27	37.41683845743735	39.0	35.0	41.0	33.5	41.0
28-29	37.279430957881644	39.0	35.0	41.0	33.0	41.0
30-31	37.03244944019904	39.0	35.0	41.0	33.0	41.0
32-33	36.52112155677981	39.0	35.0	41.0	31.5	41.0
34-35	36.048293051359515	38.0	35.0	41.0	30.0	41.0
36-37	35.79065114625911	38.0	35.0	41.0	29.5	41.0
38-39	35.67401386173805	38.0	35.0	41.0	29.0	41.0
40-41	35.50850613115337	38.0	35.0	41.0	27.0	41.0
42-43	35.42265949884485	38.0	35.0	41.0	27.0	41.0
44-45	35.28798453883064	38.0	35.0	40.5	27.0	41.0
46-47	35.16929500622001	37.5	35.0	40.0	26.0	41.0
48-49	35.05232948285054	37.0	35.0	40.0	26.0	41.0
50-51	34.804235116403056	37.0	35.0	40.0	25.0	41.0
52-53	34.614625555358096	36.0	34.0	40.0	24.0	41.0
54-55	34.40103714234939	36.0	34.0	40.0	24.5	41.0
56-57	34.09342278301048	35.5	34.0	39.5	23.5	41.0
58-59	33.81394313133109	35.0	34.0	39.0	23.0	41.0
60-61	33.419268349031455	35.0	33.5	39.0	22.0	40.5
62-63	32.97044304247379	35.0	33.0	38.0	20.5	40.0
64-65	32.56077057046383	35.0	33.0	37.5	19.5	40.0
66-67	32.16583827972276	35.0	32.5	37.0	17.5	40.0
68-69	31.63587026834903	35.0	32.0	36.5	13.0	39.0
70-71	31.291832059712103	35.0	31.5	36.0	10.5	39.0
72-73	31.01578176648303	35.0	31.0	35.5	10.0	38.0
74-75	30.728834725430957	35.0	31.0	35.0	10.0	37.0
76-77	28.843843255731294	32.5	28.0	34.5	10.0	35.5
78-79	29.823725608672472	34.0	29.5	35.0	10.0	36.0
80-81	29.969803803092233	34.0	30.0	35.0	10.0	36.0
82-83	29.78340536698063	34.0	30.0	35.0	10.0	36.0
84-85	29.561981695397193	34.0	30.0	35.0	8.0	35.0
86	29.189107517327173	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	9.0
11	21.0
12	38.0
13	113.0
14	450.0
15	1384.0
16	3880.0
17	9957.0
18	23753.0
19	36701.0
20	39449.0
21	36230.0
22	30120.0
23	25915.0
24	24795.0
25	26969.0
26	31312.0
27	37034.0
28	45103.0
29	55733.0
30	71377.0
31	87642.0
32	96695.0
33	118528.0
34	295752.0
35	321788.0
36	239407.0
37	384563.0
38	546459.0
39	221861.0
40	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.367300515372314	23.51000533143771	29.271832237426693	17.850861915763282
2	33.959516616314204	24.27218766660743	29.7687933179314	11.999502399146971
3	26.927065932113024	23.258325928558733	37.14654345121735	12.668064688110894
4	24.331402168118004	25.538333037142348	36.783579171850015	13.34668562288964
5	22.10015994313133	30.80746401279545	34.26728274391328	12.825093300159942
6	21.32383152656833	38.57618624489071	30.296214679225166	9.803767549315799
7	83.91789585925005	2.825199928914164	11.679545050648658	1.5773591611871334
8	84.54089212724365	2.3016172027723476	11.432059712102363	1.725430957881642
9	80.29521947751911	4.582974942242759	12.902861204904923	2.2189443753332148
10-11	37.804229607250754	25.308636929091875	25.29818731117825	11.588946152479117
12-13	27.619193175759733	25.813701794917364	30.399715656655413	16.167389372667497
14-15	15.010005331437712	31.904922694153193	37.57273858183757	15.51233339257153
16-17	19.663319708548073	26.686653634263376	35.479349564599254	18.1706770925893
18-19	21.59968011373734	29.01494579704994	32.31908654700551	17.066287542207213
20-21	24.221610094188733	29.585534032344057	30.83522303181091	15.357632841656299
22-23	19.377519104318463	26.992340501155144	33.16930869024347	20.460831704282924
24-25	18.000462057934957	29.299911142704815	35.777483561400395	16.922143237959837
26-27	17.11498133996801	34.98752443575617	31.09292695930336	16.80456726497245
28-29	15.931135596232451	29.930371423493867	36.36959303358806	17.76889994668562
30-31	21.028789763639594	30.564314910254133	31.651199573484984	16.755695752621293
32-33	24.112724364670342	30.382992713701796	29.67794561933535	15.826337302292517
34-35	18.437977607961614	31.280220366092053	30.847805224808955	19.433996801137372
36-37	21.671121379065223	31.582797227652392	30.242491558556956	16.50358983472543
38-39	19.133285942775903	32.72296072507553	29.296267993602278	18.847485338546292
40-41	20.25137728807535	29.90007108583615	28.86134707659499	20.987204549493512
42-43	20.377074817842544	28.983774657899414	29.476968189088325	21.162182335169717
44-45	18.867193886618093	33.112155677981164	28.69138084236716	19.329269593033587
46-47	20.164261595877022	32.11773591611871	28.093602274746754	19.62440021325751
48-49	20.085267460458503	28.601972631953082	31.579296250222143	19.733463657366272
50-51	18.150239914696996	32.0562822107695	29.72887862093478	20.06459925359872
52-53	20.79680113737338	28.748320597121023	30.612884307801668	19.841993957703927
54-55	20.444499733428113	29.548480540252353	30.96735382974942	19.039665896570106
56-57	20.719584147858537	27.46531011196019	30.695663763995025	21.119441976186245
58-59	22.0794206504354	30.225857472898525	29.034760973875954	18.659960902790118
60-61	22.411942420472723	32.29713879509507	27.053740892127244	18.23717789230496
62-63	21.30227474675671	30.33042473787098	29.71764705882353	18.64965345654878
64-65	18.988253065576686	33.73428114448196	29.246116936200462	18.03134885374089
66-67	17.899289141638526	33.01553225519815	26.57961613648481	22.505562466678512
68-69	17.812599964457082	34.4404833836858	26.290901012973166	21.45601563888395
70-71	19.001084059001244	32.88066465256797	28.321912208992355	19.79633907943842
72-73	21.220063977252533	32.999697885196376	27.307783899058112	18.47245423849298
74-75	20.26714057224098	34.0384574373556	25.457046383508086	20.237355606895328
76-77	23.636538119779633	31.228238848409458	26.532663941709618	18.6025590901013
78-79	18.579900479829394	32.24897814110538	29.537942065043538	19.63317931402168
80-81	16.739470410520703	31.462484449973342	30.185711746934423	21.612333392571532
82-83	18.566038741780698	30.070055091523013	31.038457437355603	20.32544872934068
84-85	16.942065043540076	30.093975475386532	29.37782121912209	23.586138261951305
86	18.06127599075884	30.126283987915407	30.674640127954504	21.137799893371245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	998.0
1	1718.5
2	29344.0
3	31924.5
4	8266.5
5	12387.5
6	19976.5
7	24111.0
8	26284.0
9	27212.5
10	23845.5
11	19832.0
12	16779.0
13	15127.0
14	14637.0
15	14382.5
16	13892.5
17	13631.0
18	13921.5
19	13940.5
20	13692.5
21	13742.0
22	13434.5
23	13779.5
24	15132.5
25	17257.0
26	20101.0
27	22806.0
28	25902.5
29	27714.0
30	28866.5
31	32997.5
32	37692.5
33	44578.5
34	52637.5
35	59355.5
36	63184.0
37	66905.5
38	78018.0
39	90368.5
40	100989.0
41	115405.0
42	130205.5
43	136251.0
44	142195.0
45	151074.0
46	153467.0
47	152582.0
48	147041.5
49	131662.5
50	121481.0
51	108050.0
52	91245.5
53	80616.5
54	70951.0
55	65356.0
56	59759.0
57	57347.0
58	49932.5
59	36799.5
60	26786.5
61	19098.5
62	13865.5
63	10319.0
64	8612.0
65	7002.5
66	4335.0
67	2975.0
68	2218.0
69	1279.5
70	695.0
71	594.0
72	440.5
73	240.0
74	157.0
75	97.0
76	54.0
77	35.5
78	30.5
79	25.0
80	17.5
81	9.5
82	7.0
83	4.0
84	4.5
85	4.0
86	2.0
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2813500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.553423802784913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59025332517149	14.976016824297345
2	9.112559605950231	3.563634798065671
3	3.5013809710875012	2.053919580680415
4	2.077627083289072	1.6249889145478054
5	1.3451722466107636	1.3151361512862279
6	0.9279254529621429	1.0886471783495961
7	0.6535063015758664	0.8944799970752436
8	0.49975416540994166	0.7817523994774129
9	0.34625906391805206	0.6093495200110736
>10	2.869789986497455	12.217221347745086
>50	0.8192063812580683	11.599886876816333
>100	1.2490090736778607	44.7375632311265
>500	0.004791827009322379	0.5606301478001537
>1k	0.002580214543481281	0.885433099662517
>5k	0.0	0.0
>10k+	1.843010388200915E-4	3.091339933058609
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85782	3.048942598187311	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	3060	0.10876132930513595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0662875422072152E-4	0.0	0.0	0.05494935134174516	0.0
2	1.0662875422072152E-4	0.0	0.0	0.16516794028789764	0.0
3	1.0662875422072152E-4	0.0	0.0	0.26454593922161007	0.0
4	1.0662875422072152E-4	0.0	0.0	0.43739114981339966	0.0
5	1.0662875422072152E-4	0.0	0.0	0.8940465612226763	0.0
6	1.0662875422072152E-4	0.0	0.0	1.447200995201706	0.0
7	1.0662875422072152E-4	0.0	0.0	1.9277057046383508	0.0
8	1.0662875422072152E-4	0.0	0.0	2.6075350986315975	0.0
9	1.0662875422072152E-4	0.0	0.0	2.9380842367158344	0.0
10	1.0662875422072152E-4	0.0	0.0	3.3016172027723476	0.0
11	1.0662875422072152E-4	0.0	0.0	3.836289319353119	0.0
12	1.0662875422072152E-4	0.0	0.0	4.188697352052603	0.0
13	1.0662875422072152E-4	0.0	0.0	4.317860316331971	0.0
14	1.0662875422072152E-4	0.0	0.0	4.368224631242225	0.0
15	1.0662875422072152E-4	0.0	0.0	4.435614003909721	0.0
16	1.0662875422072152E-4	0.0	0.0	4.5775368757775015	7.108583614714768E-5
17	1.0662875422072152E-4	0.0	0.0	4.768864403767549	7.108583614714768E-5
18	1.0662875422072152E-4	0.0	0.0	4.986564776968189	7.108583614714768E-5
19	1.0662875422072152E-4	0.0	0.0	5.133890172383152	7.108583614714768E-5
20	1.0662875422072152E-4	0.0	0.0	5.27638173094011	7.108583614714768E-5
21	1.0662875422072152E-4	0.0	0.0	5.450257686156033	7.108583614714768E-5
22	1.0662875422072152E-4	0.0	0.0	5.645459392216101	7.108583614714768E-5
23	1.0662875422072152E-4	0.0	0.0	5.844393104673894	7.108583614714768E-5
24	1.0662875422072152E-4	0.0	0.0	5.995699306913098	7.108583614714768E-5
25	1.0662875422072152E-4	0.0	0.0	6.1223031810911674	7.108583614714768E-5
26	1.0662875422072152E-4	0.0	0.0	6.243255731295539	7.108583614714768E-5
27	1.0662875422072152E-4	0.0	0.0	6.367869202061489	7.108583614714768E-5
28	1.0662875422072152E-4	0.0	0.0	6.5013684023458325	7.108583614714768E-5
29	1.0662875422072152E-4	0.0	0.0	6.665470055091523	7.108583614714768E-5
30	1.4217167229429537E-4	0.0	0.0	6.860387417807002	7.108583614714768E-5
31	1.4217167229429537E-4	0.0	0.0	7.022001066287542	7.108583614714768E-5
32	1.4217167229429537E-4	0.0	0.0	7.172489781411054	7.108583614714768E-5
33	1.4217167229429537E-4	0.0	0.0	7.321521236893549	7.108583614714768E-5
34	1.7771459036786921E-4	0.0	0.0	7.489070552692376	7.108583614714768E-5
35	1.7771459036786921E-4	0.0	0.0	7.682139683668029	7.108583614714768E-5
36	1.7771459036786921E-4	0.0	0.0	7.853811977963391	7.108583614714768E-5
37	1.7771459036786921E-4	0.0	0.0	8.03401457259641	7.108583614714768E-5
38	1.7771459036786921E-4	0.0	0.0	8.234618802203661	7.108583614714768E-5
39	1.7771459036786921E-4	0.0	0.0	8.538261951306202	7.108583614714768E-5
40	1.7771459036786921E-4	0.0	0.0	8.743060245246134	7.108583614714768E-5
41	1.7771459036786921E-4	0.0	0.0	8.9564243824418	7.108583614714768E-5
42	1.7771459036786921E-4	0.0	0.0	9.164492624844499	7.108583614714768E-5
43	1.7771459036786921E-4	0.0	0.0	9.366092056157811	7.108583614714768E-5
44	1.7771459036786921E-4	0.0	0.0	9.559729873822642	7.108583614714768E-5
45	1.7771459036786921E-4	0.0	0.0	9.759090101297316	7.108583614714768E-5
46	1.7771459036786921E-4	0.0	0.0	9.979527279189622	7.108583614714768E-5
47	1.7771459036786921E-4	0.0	0.0	10.20088857295184	7.108583614714768E-5
48	1.7771459036786921E-4	0.0	0.0	10.413257508441443	7.108583614714768E-5
49	1.7771459036786921E-4	0.0	0.0	10.642793673360583	7.108583614714768E-5
50	1.7771459036786921E-4	0.0	0.0	10.883312599964457	7.108583614714768E-5
51	1.7771459036786921E-4	0.0	0.0	11.115123511640306	7.108583614714768E-5
52	1.7771459036786921E-4	0.0	0.0	11.383756886440377	7.108583614714768E-5
53	1.7771459036786921E-4	0.0	0.0	11.613079793851075	7.108583614714768E-5
54	1.7771459036786921E-4	0.0	0.0	11.847272081037854	7.108583614714768E-5
55	1.7771459036786921E-4	0.0	0.0	12.085942775901902	7.108583614714768E-5
56	1.7771459036786921E-4	0.0	0.0	12.33755109294473	7.108583614714768E-5
57	1.7771459036786921E-4	0.0	0.0	12.581766483028257	7.108583614714768E-5
58	1.7771459036786921E-4	0.0	0.0	12.809703216634086	7.108583614714768E-5
59	1.7771459036786921E-4	0.0	0.0	13.056762040163498	7.108583614714768E-5
60	1.7771459036786921E-4	0.0	0.0	13.305171494579705	7.108583614714768E-5
61	1.7771459036786921E-4	0.0	0.0	13.579669450861916	7.108583614714768E-5
62	1.7771459036786921E-4	0.0	0.0	13.876701617202773	7.108583614714768E-5
63	1.7771459036786921E-4	0.0	0.0	14.204762751021859	7.108583614714768E-5
64	1.7771459036786921E-4	0.0	0.0	14.497387595521593	7.108583614714768E-5
65	1.7771459036786921E-4	0.0	0.0	14.767016172027724	7.108583614714768E-5
66	2.1325750844144304E-4	0.0	0.0	15.03252177003732	7.108583614714768E-5
67	2.1325750844144304E-4	0.0	0.0	15.291949529056335	7.108583614714768E-5
68	2.4880042651501686E-4	0.0	0.0	15.592678158876843	7.108583614714768E-5
69	2.4880042651501686E-4	0.0	0.0	15.976897103252178	7.108583614714768E-5
70	2.4880042651501686E-4	0.0	0.0	16.258610271903322	7.108583614714768E-5
71	2.4880042651501686E-4	0.0	0.0	16.53865292340501	1.0662875422072152E-4
72	2.4880042651501686E-4	0.0	0.0	16.81005864581482	1.0662875422072152E-4
73	2.4880042651501686E-4	0.0	0.0	17.092980273680467	1.0662875422072152E-4
74	2.4880042651501686E-4	0.0	0.0	17.388448551626087	1.0662875422072152E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25220	0.0	76.2728	1
GCGATCG	35	1.18210664E-7	68.57143	9
GGCGATT	885	0.0	67.344635	8
GCGGGAT	1565	0.0	66.19808	5
TAGCGGG	1700	0.0	65.411766	3
CGAGGGA	1680	0.0	65.2381	4
ACGGGAT	970	0.0	64.74227	5
CGGGATC	835	0.0	64.670654	6
TACGGGA	1010	0.0	64.55446	4
CGTAAGG	570	0.0	63.85965	2
TAGGGTA	2015	0.0	63.126553	5
ACGGGTC	515	0.0	62.91262	5
CGGGATT	1145	0.0	62.183407	6
GACCGAT	670	0.0	62.089554	9
TAAGGGA	3800	0.0	61.894737	4
ATTGCGG	745	0.0	61.74497	2
ATAGGGA	3625	0.0	61.68276	4
AGGGATT	4715	0.0	60.99682	6
TAGGGAC	2840	0.0	60.985916	5
TAGGGCG	730	0.0	60.82192	5
>>END_MODULE