FastQCFastQC Report
Thu 26 May 2016
SRR1547272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547272_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1283484
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.6070196434080986No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15680.12216747540288776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG2650.075.47169580
CGTTTTT48500.073.8969041
CGGGTAT1000.072.000016
GATACGT403.7143764E-970.000019
CTAGCGG2000.070.000012
TAGGGCG2250.069.3333365
CGTTAGG2950.069.152542
ACGGGAT5800.066.896555
GTTGACG600.066.666671
GACTTCG303.7958362E-666.666671
AGGGATC13700.064.817516
AAGTACT40500.064.4938380
CGTAAGG1700.063.529412
GAGGGAT18100.063.425415
GCGTAGG2650.063.3962252
TAGCACG1200.063.3333361
CGGGATA2850.063.1578986
AGCGGGA9500.063.1578944
CGTAGGG5800.062.7586253
ATAGGGA14050.062.633454
Produced by FastQC (version 0.11.5)