FastQCFastQC Report
Thu 26 May 2016
SRR1547271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547271_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3076511
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307090.9981761807450062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168200.075.481571
AGTACGG5300.068.6792452
GGGCGAT21100.067.109017
AATCGCG850.065.8823551
CGAGGGA23250.064.8602144
CGTTAGG4950.063.8383872
TACGGGA13350.063.5205964
CGGGTAT2150.063.255816
ACGGGAT16400.063.1707275
TAGGGAC32100.061.931465
TATTGCG2600.061.538461
GTACGGG13150.061.4448623
TAAGGGA44050.061.3847924
GCGGGAT21500.060.651165
CGTAAGG5550.060.5405432
GCGATAA2450.060.408169
ATAGGGA42550.060.0705074
AGCGGGA23050.059.5227784
TAGCGGG20000.059.43
GGCGATA4850.059.3814438
Produced by FastQC (version 0.11.5)