FastQCFastQC Report
Thu 26 May 2016
SRR1547270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547270_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1164531
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.7232954725979815No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA34620.29728706234527036No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27580.23683354071295654No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18060.15508389214198678No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA13060.11214815234630937No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA11830.10158596035657273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG251.2848159E-680.000011
TGCGTTG251.2848159E-680.000011
CGTATGG700.074.285712
CGGGACG950.071.578956
CGTTTTT50800.070.866141
CGTGCGG1300.070.7692262
ATAGCCG403.7143764E-970.000011
CGTAAGG2600.069.230772
TACGGGA3600.068.888894
TAGTAAG2350.068.0851061
ACGTGCG650.067.692311
AACGGGA4200.067.619054
AAGTACT41700.067.3381380
ATCGTAG600.066.6666641
TATAGCG303.795616E-666.6666641
TGCAACG303.795616E-666.6666641
TAGTAGG5450.066.0550462
TAGCACG559.094947E-1265.4545441
TAAGGGA13150.065.0950554
GTGCGGG5050.064.950493
Produced by FastQC (version 0.11.5)