##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547270_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1164531
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22045097983652	33.0	31.0	34.0	31.0	34.0
2	32.64761350277494	34.0	31.0	34.0	31.0	34.0
3	32.73169284458722	34.0	31.0	34.0	31.0	34.0
4	36.23801427355734	37.0	37.0	37.0	35.0	37.0
5	36.21864252647632	37.0	37.0	37.0	35.0	37.0
6	36.27363805686581	37.0	37.0	37.0	35.0	37.0
7	36.434522567454195	37.0	37.0	37.0	35.0	37.0
8	36.42173458671345	37.0	37.0	37.0	35.0	37.0
9	38.18790053678262	39.0	39.0	39.0	37.0	39.0
10-11	37.86702844321019	39.0	37.5	39.0	35.0	39.0
12-13	36.77885689603798	39.0	35.0	39.0	34.0	39.0
14-15	37.81340470970717	40.0	35.0	41.0	33.0	41.0
16-17	38.0451164460199	40.0	35.0	41.0	34.0	41.0
18-19	37.92376673527798	39.0	36.0	41.0	34.0	41.0
20-21	37.67143510992837	39.0	35.0	41.0	34.0	41.0
22-23	37.47844325312079	39.0	35.0	41.0	34.0	41.0
24-25	37.46543501203489	39.0	35.0	41.0	34.0	41.0
26-27	37.424246756848895	39.0	35.0	41.0	34.0	41.0
28-29	37.37996326418103	39.0	35.0	41.0	34.0	41.0
30-31	37.24774952319861	39.0	35.0	41.0	34.0	41.0
32-33	36.852808555547256	39.0	35.0	41.0	33.0	41.0
34-35	36.50532875466604	38.0	35.0	41.0	32.5	41.0
36-37	36.27565603663621	38.0	35.0	41.0	31.5	41.0
38-39	36.223602033780125	38.0	35.0	41.0	31.0	41.0
40-41	36.09029944243648	38.0	35.0	41.0	31.0	41.0
42-43	36.03171748970186	38.0	35.0	41.0	31.0	41.0
44-45	35.884284317034066	38.0	35.0	41.0	31.0	41.0
46-47	35.767130286784976	37.0	35.0	40.0	31.0	41.0
48-49	35.71270322559039	37.0	35.0	40.0	31.0	41.0
50-51	35.48812096887073	36.5	35.0	40.0	30.0	41.0
52-53	35.29354907683866	36.0	35.0	40.0	30.0	41.0
54-55	35.147230945333355	36.0	35.0	40.0	29.5	41.0
56-57	34.877745203863185	35.5	34.5	40.0	29.0	41.0
58-59	34.64712918763004	35.0	34.0	39.0	28.0	41.0
60-61	34.299686311485054	35.0	34.0	39.0	27.5	41.0
62-63	33.850873871112064	35.0	33.5	38.5	26.0	40.0
64-65	33.522625846800125	35.0	33.5	37.5	26.0	40.0
66-67	33.260677903808485	35.0	34.0	37.0	25.0	40.0
68-69	32.923271256840735	35.0	33.5	36.5	24.0	39.0
70-71	32.65437502307796	35.0	33.0	36.0	23.5	39.0
72-73	32.40212111141739	35.0	33.0	36.0	23.0	38.5
74-75	32.14535336543209	35.0	33.0	35.0	22.5	37.0
76-77	30.23290105630507	33.0	30.0	34.5	19.0	35.5
78-79	31.147492423988716	34.0	31.5	35.0	19.0	36.0
80-81	31.441687683711297	35.0	32.5	35.0	19.0	36.0
82-83	31.311786891031666	35.0	32.5	35.0	19.0	36.0
84-85	31.127989293543926	35.0	32.0	35.0	18.0	35.5
86	30.735146595496385	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	24.0
12	18.0
13	34.0
14	134.0
15	380.0
16	1049.0
17	2332.0
18	5389.0
19	8931.0
20	11367.0
21	11693.0
22	9762.0
23	7933.0
24	7452.0
25	7636.0
26	8415.0
27	10266.0
28	12761.0
29	16409.0
30	21837.0
31	27742.0
32	36361.0
33	51302.0
34	171168.0
35	153873.0
36	90794.0
37	166297.0
38	225371.0
39	97633.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.273593403696424	22.804974706555686	29.88705324289349	15.0343786468544
2	31.38078763038511	25.365061127612748	29.963908217127756	13.29024302487439
3	29.732742194067825	24.06213316777312	33.0595750563961	13.145549581762959
4	26.518916198881783	26.71152592760519	32.07462918548325	14.694928688029773
5	24.190596901241786	32.05573745997315	29.674263716466115	14.079401922318944
6	22.97122189104455	40.24804835594759	26.342879665719504	10.437850087288359
7	85.81162717007963	4.227968169159945	7.906874097812768	2.053530562947659
8	86.03008421416003	4.115218916456496	7.710657766946522	2.1440391024369467
9	81.15309940224863	6.81622043552297	8.950212574847729	3.080467587380671
10-11	47.36022484588216	24.59427872680075	18.358420686095947	9.687075741221143
12-13	33.21032243881872	27.745590284844283	24.55709637613769	14.486990900199306
14-15	14.816436831651542	34.257181646516926	36.21943082665897	14.706950695172564
16-17	17.968392425792015	23.07688674668171	36.49988707900434	22.45483374852194
18-19	23.750376761116705	26.001025305466317	30.385193696002936	19.86340423741403
20-21	27.434220299846036	26.575848989850847	28.6811171192523	17.308813591050818
22-23	21.231122228605333	25.780722024574697	28.970332262515985	24.01782348430398
24-25	18.903575774281663	26.35404295806638	35.723651839238286	19.018729428413668
26-27	16.997443606052563	36.09663461084334	29.512567720395595	17.393354062708507
28-29	15.527280939708776	28.757456864609015	37.71436741486487	18.00089478081734
30-31	22.66358731540852	28.759947137517162	30.664147197455456	17.912318349618857
32-33	26.751241486916193	29.546701633533157	26.904393270767375	16.797663608783278
34-35	20.514352988456295	29.32695651725888	28.72491157384389	21.433778920440933
36-37	23.17426500453831	30.097953596769862	29.822349083021404	16.905432315670428
38-39	20.354159743278625	31.05696628084611	27.823175166655073	20.765698809220194
40-41	20.160648364019508	28.33222129767263	28.749513752746815	22.757616585561056
42-43	23.057866213952227	26.375124406306057	26.37405101281117	24.19295836693055
44-45	18.91263521537855	34.48233666600546	26.10505860299125	20.499969515624745
46-47	21.664987879240655	30.706052479496037	26.828697561507592	20.80026207975571
48-49	21.849869174800844	25.37485047628616	31.459875263088744	21.315405085824253
50-51	18.69739835178282	31.982145601963364	27.686510706885432	21.63394533936838
52-53	22.439806239593448	26.586926410718135	30.11671651506057	20.856550834627846
54-55	22.70918507107153	27.128217282322243	31.307839808472256	18.854757838133978
56-57	23.34656612833836	25.289580097051946	28.879351429888946	22.48450234472075
58-59	24.22859503096096	26.15580864742974	28.01853278272541	21.597063538883894
60-61	25.046778488507393	29.262166485907205	26.36052625477553	19.33052877080988
62-63	24.39836294611307	25.14076482292013	31.15468802462107	19.30618420634573
64-65	18.950332794919156	32.454953968593365	29.79684525358277	18.797867982904705
66-67	18.766567828593658	28.859257503664566	25.386228447332016	26.987946220409764
68-69	17.728939804951523	33.26382037060413	23.792024428718513	25.21521539572583
70-71	20.173056792820457	30.205722303657005	27.95584660262372	21.66537430089882
72-73	22.985905914054673	31.517366218675157	25.54822499358111	19.948502873689065
74-75	21.745063033959593	32.76314670884673	24.288318645016748	21.20347161217692
76-77	27.6560263316305	28.192680143336673	24.78993689304965	19.361356631983178
78-79	19.595528156828802	30.64641473691984	29.014083781367777	20.74397332488358
80-81	15.76295521544725	29.111762589402947	31.24803032293687	23.877251872212934
82-83	19.07347249665316	26.46133937181578	31.956427093825756	22.50876103770531
84-85	17.02835733870545	26.398910806152863	29.725142568123992	26.84758928701769
86	18.346613357652135	26.728957837962238	32.341689487012374	22.58273931737326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	761.0
1	926.0
2	3303.5
3	3750.0
4	2151.5
5	2543.0
6	3126.5
7	3486.0
8	3553.5
9	3502.0
10	3220.0
11	3043.0
12	3000.5
13	2974.0
14	2976.0
15	2956.5
16	3021.0
17	3192.0
18	3451.5
19	3457.5
20	3592.5
21	3894.0
22	3635.5
23	3950.0
24	4666.0
25	5586.0
26	7268.5
27	11172.5
28	14183.0
29	14195.0
30	17380.0
31	21704.5
32	19863.0
33	18281.0
34	20266.0
35	23090.5
36	25329.0
37	25277.0
38	27020.0
39	30907.0
40	37392.0
41	47202.0
42	58218.0
43	63817.0
44	63946.0
45	67190.5
46	70969.0
47	73700.5
48	71455.0
49	57674.5
50	48208.0
51	42645.5
52	34138.0
53	29933.0
54	29046.0
55	29165.5
56	27616.0
57	26320.0
58	26545.5
59	21308.0
60	14907.5
61	13633.0
62	12316.0
63	9415.5
64	7495.0
65	6126.5
66	3561.0
67	2019.5
68	1520.5
69	1220.5
70	857.0
71	639.0
72	511.0
73	329.0
74	218.5
75	135.5
76	73.5
77	37.5
78	33.0
79	29.0
80	24.0
81	17.0
82	12.0
83	7.0
84	3.0
85	2.5
86	4.0
87	3.0
88	1.5
89	1.0
90	1.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1164531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.255443367046258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.39265100235242	13.87210836497807
2	8.213751679011505	2.834638538563283
3	2.5926823667548082	1.3421365124483113
4	1.1999540626510878	0.828229574845317
5	0.7377060512981158	0.6364722494850981
6	0.4589391493853463	0.4751519100683536
7	0.36543949417942356	0.4414074347126552
8	0.2812633748363287	0.3882659388570059
9	0.20340223338742563	0.3158816147052703
>10	2.401949414497934	10.574783330512217
>50	1.3063243618550637	16.544118176715017
>100	1.8378978906406325	49.4669367565521
>500	0.005024324468673134	0.638002828019319
>1k	0.002512162234336567	0.9116183906268704
>5k	5.024324468673135E-4	0.7302483789110917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8423	0.7232954725979815	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3462	0.29728706234527036	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2758	0.23683354071295654	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1806	0.15508389214198678	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1306	0.11214815234630937	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1183	0.10158596035657273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.587147959135481E-5	0.0	0.0	0.04774454265279327	0.0
2	8.587147959135481E-5	0.0	0.0	0.19441302979482727	0.0
3	8.587147959135481E-5	0.0	0.0	0.2920489020901977	0.0
4	8.587147959135481E-5	0.0	0.0	0.4515981111709349	0.0
5	8.587147959135481E-5	0.0	0.0	0.7481982016794744	0.0
6	8.587147959135481E-5	0.0	0.0	0.9931036614740183	0.0
7	8.587147959135481E-5	0.0	0.0	1.1499908546874236	0.0
8	8.587147959135481E-5	0.0	0.0	1.4650533133081043	0.0
9	8.587147959135481E-5	0.0	0.0	1.5741959638687162	0.0
10	8.587147959135481E-5	0.0	0.0	1.7601077171839994	0.0
11	8.587147959135481E-5	0.0	0.0	2.1158732571309824	0.0
12	8.587147959135481E-5	0.0	0.0	2.3799280568743986	0.0
13	8.587147959135481E-5	0.0	0.0	2.476876957333038	0.0
14	8.587147959135481E-5	0.0	0.0	2.5201561830470807	0.0
15	8.587147959135481E-5	0.0	0.0	2.5849032786589623	0.0
16	8.587147959135481E-5	0.0	0.0	2.727449934780611	0.0
17	8.587147959135481E-5	0.0	0.0	2.892494918555195	0.0
18	8.587147959135481E-5	0.0	0.0	3.150710457686399	0.0
19	8.587147959135481E-5	0.0	0.0	3.280376391869345	0.0
20	8.587147959135481E-5	0.0	0.0	3.424211120184864	0.0
21	8.587147959135481E-5	0.0	0.0	3.5895995898778135	0.0
22	8.587147959135481E-5	0.0	0.0	3.7618577779380713	0.0
23	8.587147959135481E-5	0.0	0.0	3.9543816351818886	0.0
24	8.587147959135481E-5	0.0	0.0	4.0930640747219265	0.0
25	8.587147959135481E-5	0.0	0.0	4.205383970027419	0.0
26	8.587147959135481E-5	0.0	0.0	4.3220833107920695	0.0
27	8.587147959135481E-5	0.0	0.0	4.4327716479853265	0.0
28	8.587147959135481E-5	0.0	0.0	4.553163462372406	0.0
29	8.587147959135481E-5	0.0	0.0	4.682829396555351	0.0
30	8.587147959135481E-5	0.0	0.0	4.84813199476871	0.0
31	8.587147959135481E-5	0.0	0.0	5.020218439869785	0.0
32	8.587147959135481E-5	0.0	0.0	5.16826087068528	0.0
33	8.587147959135481E-5	0.0	0.0	5.316303301500776	0.0
34	8.587147959135481E-5	0.0	0.0	5.460567387214252	0.0
35	8.587147959135481E-5	0.0	0.0	5.646393269049944	0.0
36	8.587147959135481E-5	0.0	0.0	5.8214852159367165	0.0
37	8.587147959135481E-5	0.0	0.0	6.004391467466302	0.0
38	8.587147959135481E-5	0.0	0.0	6.170638651955165	0.0
39	8.587147959135481E-5	0.0	0.0	6.345215369964389	0.0
40	8.587147959135481E-5	0.0	0.0	6.52717703521847	0.0
41	8.587147959135481E-5	0.0	0.0	6.725883638992865	0.0
42	8.587147959135481E-5	0.0	0.0	6.9208977691448315	0.0
43	8.587147959135481E-5	0.0	0.0	7.092297242409176	0.0
44	8.587147959135481E-5	0.0	0.0	7.266788088938809	0.0
45	1.7174295918270961E-4	0.0	0.0	7.449179111590847	0.0
46	1.7174295918270961E-4	0.0	0.0	7.665231754242695	0.0
47	1.7174295918270961E-4	0.0	0.0	7.893134661078151	0.0
48	2.576144387740644E-4	0.0	0.0	8.096220710311705	0.0
49	2.576144387740644E-4	0.0	0.0	8.293381627453455	0.0
50	2.576144387740644E-4	0.0	0.0	8.50788858347266	0.0
51	2.576144387740644E-4	0.0	0.0	8.712348576379675	0.0
52	2.576144387740644E-4	0.0	0.0	8.947207073062032	0.0
53	2.576144387740644E-4	0.0	0.0	9.155187796632292	0.0
54	2.576144387740644E-4	0.0	0.0	9.384636390100392	0.0
55	2.576144387740644E-4	0.0	0.0	9.61520131280318	0.0
56	2.576144387740644E-4	0.0	0.0	9.851777239077363	0.0
57	2.576144387740644E-4	0.0	0.0	10.071865841270006	0.0
58	2.576144387740644E-4	0.0	0.0	10.283453166983103	0.0
59	2.576144387740644E-4	0.0	0.0	10.504056998053294	0.0
60	2.576144387740644E-4	0.0	0.0	10.74913420080702	0.0
61	2.576144387740644E-4	0.0	0.0	11.000136535652551	0.0
62	2.576144387740644E-4	0.0	0.0	11.28411351866116	0.0
63	2.576144387740644E-4	0.0	0.0	11.61179908478177	0.0
64	2.576144387740644E-4	0.0	0.0	11.89569019631079	0.0
65	2.576144387740644E-4	0.0	0.0	12.13655969656454	0.0
66	2.576144387740644E-4	0.0	0.0	12.362144073451029	0.0
67	2.576144387740644E-4	0.0	0.0	12.61709649635776	0.0
68	2.576144387740644E-4	0.0	0.0	12.895663576152117	0.0
69	2.576144387740644E-4	0.0	0.0	13.188485321558636	0.0
70	2.576144387740644E-4	0.0	0.0	13.426864548904238	0.0
71	2.576144387740644E-4	0.0	0.0	13.662495888902914	0.0
72	2.576144387740644E-4	0.0	0.0	13.937542238034023	0.0
73	2.576144387740644E-4	0.0	0.0	14.192924018338713	0.0
74	2.576144387740644E-4	0.0	0.0	14.512537665377735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	25	1.2848159E-6	80.00001	1
TGCGTTG	25	1.2848159E-6	80.00001	1
CGTATGG	70	0.0	74.28571	2
CGGGACG	95	0.0	71.57895	6
CGTTTTT	5080	0.0	70.86614	1
CGTGCGG	130	0.0	70.769226	2
ATAGCCG	40	3.7143764E-9	70.00001	1
CGTAAGG	260	0.0	69.23077	2
TACGGGA	360	0.0	68.88889	4
TAGTAAG	235	0.0	68.085106	1
ACGTGCG	65	0.0	67.69231	1
AACGGGA	420	0.0	67.61905	4
AAGTACT	4170	0.0	67.33813	80
ATCGTAG	60	0.0	66.666664	1
TATAGCG	30	3.795616E-6	66.666664	1
TGCAACG	30	3.795616E-6	66.666664	1
TAGTAGG	545	0.0	66.055046	2
TAGCACG	55	9.094947E-12	65.454544	1
TAAGGGA	1315	0.0	65.095055	4
GTGCGGG	505	0.0	64.95049	3
>>END_MODULE