FastQCFastQC Report
Thu 26 May 2016
SRR1547269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547269_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2782862
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135530.48701660376978806No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC29460.1058622382281263TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91350.073.256711
GCGTAAG1850.071.351351
CGTAAGG5150.068.349512
TAGCGGG21150.066.382983
TAGGGTA24050.066.195425
CGAGGGA11450.065.676854
ATAGCGG7150.064.89512
AGGGTAA23900.064.100426
TTGCGGG24750.064.03
ATACGCG251.2358339E-464.01
TAGTCCG503.0013325E-1064.01
TAGGGCG4650.063.6559145
CGGGTAT4350.063.4482776
TACGGGT6750.063.4074064
TATAGCG1900.063.157891
GATTACG950.063.157891
CGGGATC8300.062.65066
ACGGGTC6600.062.424245
ATAGGGT15450.062.1359184
CGCGAGG4000.062.02
Produced by FastQC (version 0.11.5)