##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547269_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2782862
Sequences flagged as poor quality	0
Sequence length	86
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13589067657685	33.0	31.0	34.0	31.0	34.0
2	32.58787931273631	34.0	31.0	34.0	31.0	34.0
3	32.7412530696815	34.0	31.0	34.0	31.0	34.0
4	36.24657672568744	37.0	37.0	37.0	35.0	37.0
5	36.22379298721963	37.0	37.0	37.0	35.0	37.0
6	36.27772056250004	37.0	37.0	37.0	35.0	37.0
7	36.43796745939971	37.0	37.0	37.0	35.0	37.0
8	36.43532305949774	37.0	37.0	37.0	35.0	37.0
9	38.2738529614476	39.0	39.0	39.0	37.0	39.0
10-11	37.81301803682683	39.0	37.5	39.0	35.0	39.0
12-13	37.09611364128009	39.0	35.0	39.0	34.0	39.0
14-15	38.22628251059521	40.0	36.0	41.0	33.5	41.0
16-17	38.379400595502034	40.0	36.0	41.0	35.0	41.0
18-19	38.27409569716357	40.0	36.0	41.0	34.0	41.0
20-21	38.10525836351209	40.0	35.0	41.0	34.0	41.0
22-23	37.941254363313746	40.0	35.0	41.0	34.0	41.0
24-25	37.94063377918129	40.0	35.0	41.0	34.0	41.0
26-27	37.944408130909835	40.0	35.0	41.0	34.0	41.0
28-29	37.88541634475587	40.0	35.0	41.0	34.0	41.0
30-31	37.752569836377084	40.0	35.0	41.0	34.0	41.0
32-33	37.4374674346051	40.0	35.0	41.0	33.5	41.0
34-35	37.14876842617421	40.0	35.0	41.0	33.0	41.0
36-37	36.992818185019594	40.0	35.0	41.0	33.0	41.0
38-39	36.909917380021	39.0	35.0	41.0	33.0	41.0
40-41	36.78864079498013	39.0	35.0	41.0	32.0	41.0
42-43	36.75164435031273	39.0	35.0	41.0	32.0	41.0
44-45	36.6241718058603	39.0	35.0	41.0	32.0	41.0
46-47	36.494036894391456	39.0	35.0	41.0	32.0	41.0
48-49	36.409680573452796	38.0	35.0	41.0	32.0	41.0
50-51	36.20350901338263	38.0	35.0	40.0	31.0	41.0
52-53	36.020078969061345	38.0	35.0	40.0	31.0	41.0
54-55	35.82966384966268	37.0	35.0	40.0	31.0	41.0
56-57	35.53431269678482	37.0	35.0	40.0	30.5	41.0
58-59	35.27698876192927	36.0	35.0	40.0	30.0	41.0
60-61	34.890446418112006	36.0	34.5	39.0	29.0	41.0
62-63	34.44516436675624	35.0	34.0	39.0	27.5	40.5
64-65	34.03313189802441	35.0	34.0	38.5	27.0	40.0
66-67	33.675189067945155	35.0	34.0	37.5	26.0	40.0
68-69	33.15024640100731	35.0	33.5	37.0	24.5	39.5
70-71	32.811243424934474	35.0	33.0	36.5	24.0	39.0
72-73	32.51607176352978	35.0	33.0	36.0	23.0	38.5
74-75	32.19785584049802	35.0	33.0	35.5	21.5	37.5
76-77	30.308695328765854	33.0	30.0	34.5	19.0	35.5
78-79	31.24226749296228	34.0	31.5	35.0	19.0	36.0
80-81	31.364536581404323	35.0	32.0	35.0	18.5	36.0
82-83	31.14733734550977	35.0	32.0	35.0	17.5	36.0
84-85	30.901319936094566	34.0	32.0	35.0	15.0	35.5
86	30.522484765683675	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	9.0
12	24.0
13	91.0
14	232.0
15	657.0
16	1699.0
17	4381.0
18	10580.0
19	18158.0
20	21706.0
21	21333.0
22	18059.0
23	15154.0
24	14501.0
25	15864.0
26	18785.0
27	23838.0
28	30524.0
29	41134.0
30	54729.0
31	70528.0
32	88386.0
33	117162.0
34	321724.0
35	298669.0
36	238706.0
37	447269.0
38	635988.0
39	252624.0
40	340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.572518507924574	24.13163139242981	31.318584967562174	13.977265132083444
2	28.3226045704027	26.222787906838356	32.22236675767609	13.232240765082853
3	28.655822674642152	24.736440398409982	32.80712446395114	13.800612462996728
4	26.388408767664366	26.9886900608079	32.53865984012143	14.0842413314063
5	24.55540375340207	31.81185412715399	29.715702754933588	13.91703936451035
6	22.14604964241849	41.0920484019689	25.80325578487183	10.958646170740769
7	89.61256433125322	3.0613806936887276	5.87521048474556	1.4508444903124913
8	90.33908257038976	2.255268137622347	5.79475374632303	1.6108955456648588
9	86.51503380332909	3.787503656307787	7.467096823342301	2.230365717020822
10-11	40.986258032198506	23.971993580709356	21.62949510252395	13.412253284568191
12-13	30.898118555645233	25.45947301734689	25.522411819199082	18.119996607808794
14-15	17.65788242464053	30.39809376102732	33.87526941688089	18.068754397451254
16-17	23.516365525850723	24.636669011974003	31.336013068560355	20.510952393614918
18-19	25.675886910669664	26.687938532345477	28.522704323822023	19.11347023316284
20-21	27.036788026139995	27.33011913634237	28.219832675856726	17.413260161660908
22-23	22.594041673643897	23.519869113164795	31.251046584415615	22.6350426287757
24-25	21.005551119674635	25.92309643812737	32.89622338441504	20.17512905778296
26-27	19.003044347869206	32.10753892934684	28.496310632722714	20.393106090061238
28-29	16.8699885226073	28.34405730503345	33.974268217396336	20.811685954962915
30-31	22.903273680117806	27.83366548538878	29.24897102335653	20.014089811136877
32-33	25.51102066864976	27.236995582245903	27.849566381660317	19.40241736744402
34-35	19.61919060305542	28.217658655010563	29.292325670478807	22.870825071455215
36-37	22.26781277691815	29.19268005384385	28.539683246959424	19.999823922278576
38-39	20.60637573835857	30.151566983917995	27.07419555838558	22.16786171933786
40-41	20.97784582922186	26.16346049498682	27.535662925434316	25.323030750357006
42-43	21.166770037465028	25.791163916859695	27.63739272734329	25.404673318331987
44-45	20.017431694421067	30.214362048854742	26.60735602412193	23.160850232602264
46-47	21.03672406321262	28.993676294404825	27.441227771984384	22.52837187039817
48-49	21.255886925043356	25.628813070860147	30.313217112454733	22.80208289164177
50-51	19.295261496976853	29.264009498135373	28.184958506745932	23.255770498141842
52-53	22.03262324901486	25.589465090256002	29.628993460689028	22.7489182000401
54-55	21.483188889711382	26.417066315181998	30.15857415854613	21.941170636560493
56-57	22.05159652185412	25.18464803500856	28.55605128820617	24.207704154931147
58-59	23.16677938036453	27.655270006202247	27.314865056190353	21.86308555724287
60-61	24.155833095568518	28.23007393108246	26.14339122816726	21.47070174518176
62-63	22.288654629658243	26.000301128837865	29.82399055360992	21.887053687893975
64-65	19.655627911121716	29.933500116067556	28.717521745598596	21.69335022721213
66-67	19.11740503122325	29.654650500096665	25.544798843780253	25.683145624899833
68-69	18.824972276742432	31.207081055402675	25.41994536559844	24.548001302256452
70-71	19.29668089901691	29.428785904583123	28.293138502735673	22.981394693664292
72-73	21.33222919426116	30.678219042122823	26.329225092728276	21.66032667088774
74-75	20.30700767770734	31.95573477951835	24.631728055505448	23.10552948726886
76-77	23.541681190084166	28.243315694418193	25.312717626673546	22.902285488824095
78-79	18.848167821473	29.872968907549136	27.818034095833717	23.46082917514415
80-81	17.296168476913337	29.394127340845504	28.347902267521707	24.96180191471945
82-83	18.871004023914946	28.432491442263398	29.08662017735698	23.609884356464676
84-85	17.36440757752271	28.05124724114958	27.794982288018595	26.78936289330912
86	17.618911753439445	28.826797735568633	28.858276120051947	24.696014390939975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	988.0
1	1219.5
2	4084.0
3	4543.0
4	2706.0
5	3472.5
6	4194.5
7	4487.0
8	4736.0
9	4988.5
10	5034.5
11	4996.0
12	4933.5
13	5054.0
14	5156.0
15	5245.0
16	5421.0
17	5495.0
18	5521.0
19	5840.5
20	6248.0
21	6375.0
22	6807.5
23	8034.5
24	9657.0
25	9779.5
26	10574.0
27	12968.0
28	18439.5
29	23017.0
30	25410.5
31	29979.0
32	36128.0
33	40549.5
34	46653.0
35	56438.0
36	60567.0
37	62020.5
38	70001.0
39	83943.5
40	100200.5
41	119683.0
42	138114.0
43	145904.0
44	150494.0
45	153818.0
46	157456.0
47	167043.5
48	168905.0
49	155316.0
50	144549.0
51	131878.0
52	112253.0
53	102589.5
54	98167.5
55	90953.5
56	82424.5
57	79397.0
58	68676.0
59	51165.0
60	37836.5
61	26678.5
62	19534.5
63	13753.5
64	10497.0
65	8382.5
66	5106.0
67	3575.0
68	2527.5
69	1574.0
70	1082.5
71	866.0
72	649.5
73	483.0
74	362.0
75	196.5
76	167.5
77	95.0
78	37.0
79	17.0
80	18.0
81	18.5
82	15.0
83	9.0
84	5.0
85	2.0
86	0.0
87	1.5
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2782862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.50893828173348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71750746680544	13.432421033647241
2	10.114445202084278	3.5418639359453796
3	3.4495125117140235	1.8119190500900484
4	1.673792951917975	1.172253499661293
5	0.9461237996333255	0.8282811607329534
6	0.6033129685782364	0.6338021718843451
7	0.4613791356303399	0.5654781167161813
8	0.3444650610708833	0.48249739956029203
9	0.26723554845483005	0.4211109652122734
>10	2.5771781359118116	10.698781649941676
>50	1.187011572727322	15.224808290380631
>100	1.650581939414457	49.55135528129688
>500	0.005383232152358876	0.6380679284957159
>1k	0.0018634265142780724	0.5060388735794386
>5k	0.0	0.0
>10k+	2.0704739047534138E-4	0.4913206428556581
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13553	0.48701660376978806	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2946	0.1058622382281263	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.593422886222888E-5	0.0	0.0	0.11056962220907827	0.0
2	3.593422886222888E-5	0.0	0.0	0.3127715280168402	0.0
3	3.593422886222888E-5	0.0	0.0	0.45180105948480376	0.0
4	3.593422886222888E-5	0.0	0.0	0.6654300500707545	0.0
5	3.593422886222888E-5	0.0	0.0	1.1239148761239328	0.0
6	3.593422886222888E-5	0.0	0.0	1.4812807821587992	0.0
7	3.593422886222888E-5	0.0	0.0	1.723513418919084	0.0
8	3.593422886222888E-5	0.0	0.0	2.1938206062679355	0.0
9	3.593422886222888E-5	0.0	0.0	2.3410431419164874	0.0
10	3.593422886222888E-5	0.0	0.0	2.6672900057566635	0.0
11	3.593422886222888E-5	0.0	0.0	3.1904564437618537	0.0
12	7.186845772445777E-5	0.0	0.0	3.598309941348152	0.0
13	1.0780268658668666E-4	0.0	0.0	3.7534020731175315	0.0
14	1.0780268658668666E-4	0.0	0.0	3.821317765667144	0.0
15	1.0780268658668666E-4	0.0	0.0	3.931995190562809	0.0
16	1.0780268658668666E-4	0.0	0.0	4.163878769410772	0.0
17	1.0780268658668666E-4	0.0	0.0	4.438811554435685	0.0
18	1.0780268658668666E-4	0.0	0.0	4.778318148726024	0.0
19	1.0780268658668666E-4	0.0	0.0	4.986449202296053	0.0
20	1.0780268658668666E-4	0.0	0.0	5.223327638955866	0.0
21	1.0780268658668666E-4	0.0	0.0	5.513819945078125	0.0
22	1.0780268658668666E-4	0.0	0.0	5.811786570803727	0.0
23	1.0780268658668666E-4	0.0	0.0	6.093187517023841	0.0
24	1.0780268658668666E-4	0.0	0.0	6.314326761441998	0.0
25	1.0780268658668666E-4	0.0	0.0	6.519475274016462	0.0
26	1.0780268658668666E-4	0.0	0.0	6.714849676340401	0.0
27	1.0780268658668666E-4	0.0	0.0	6.940265093993163	0.0
28	1.0780268658668666E-4	0.0	0.0	7.148144607961156	0.0
29	1.0780268658668666E-4	0.0	0.0	7.400438828802866	0.0
30	1.0780268658668666E-4	0.0	0.0	7.679324379002624	0.0
31	1.4373691544891553E-4	0.0	0.0	7.928312650789008	0.0
32	1.4373691544891553E-4	0.0	0.0	8.15002684286896	0.0
33	1.4373691544891553E-4	0.0	0.0	8.383060317040515	0.0
34	1.4373691544891553E-4	0.0	0.0	8.624071190019484	0.0
35	1.4373691544891553E-4	0.0	0.0	8.894332525292308	0.0
36	1.4373691544891553E-4	0.0	0.0	9.13347481837044	0.0
37	1.4373691544891553E-4	0.0	0.0	9.407365510758348	0.0
38	1.4373691544891553E-4	0.0	0.0	9.693797249019175	0.0
39	1.4373691544891553E-4	0.0	0.0	10.072292481625032	0.0
40	1.4373691544891553E-4	0.0	0.0	10.371552739589674	0.0
41	1.4373691544891553E-4	0.0	0.0	10.65521754222811	0.0
42	1.4373691544891553E-4	0.0	0.0	10.955124616312272	0.0
43	1.4373691544891553E-4	0.0	0.0	11.217624158150853	0.0
44	1.4373691544891553E-4	0.0	0.0	11.482100082576858	0.0
45	1.4373691544891553E-4	0.0	0.0	11.734969251080363	0.0
46	1.4373691544891553E-4	0.0	0.0	12.030672020387644	0.0
47	1.4373691544891553E-4	0.0	0.0	12.318612996260685	0.0
48	1.4373691544891553E-4	0.0	0.0	12.601056035117804	0.0
49	1.4373691544891553E-4	0.0	0.0	12.900927174973104	0.0
50	1.4373691544891553E-4	0.0	0.0	13.196342470449487	0.0
51	1.4373691544891553E-4	0.0	0.0	13.484463117466838	0.0
52	1.4373691544891553E-4	0.0	0.0	13.820735631159575	0.0
53	1.4373691544891553E-4	0.0	0.0	14.110617055391176	0.0
54	1.4373691544891553E-4	0.0	0.0	14.413255130868867	0.0
55	1.4373691544891553E-4	0.0	0.0	14.717833654705121	0.0
56	1.4373691544891553E-4	0.0	0.0	15.023562073864964	0.0
57	1.4373691544891553E-4	0.0	0.0	15.33611799650863	0.0
58	1.4373691544891553E-4	0.0	0.0	15.627868000641067	0.0
59	1.4373691544891553E-4	0.0	0.0	15.937441382289169	0.0
60	1.4373691544891553E-4	0.0	0.0	16.26167592931306	0.0
61	1.4373691544891553E-4	0.0	0.0	16.59298951942281	0.0
62	1.4373691544891553E-4	0.0	0.0	17.007526783577482	0.0
63	1.4373691544891553E-4	0.0	0.0	17.391879295487882	0.0
64	1.4373691544891553E-4	0.0	0.0	17.74716101624874	0.0
65	1.4373691544891553E-4	0.0	0.0	18.083685069543513	0.0
66	1.4373691544891553E-4	0.0	0.0	18.384059288602884	0.0
67	1.7967114431114442E-4	0.0	0.0	18.71641497134964	0.0
68	1.7967114431114442E-4	0.0	0.0	19.065048859770982	0.0
69	1.7967114431114442E-4	0.0	0.0	19.533954612194208	0.0
70	1.7967114431114442E-4	0.0	0.0	19.879318485789092	0.0
71	1.7967114431114442E-4	0.0	0.0	20.213399011521233	0.0
72	1.7967114431114442E-4	0.0	0.0	20.567854245018257	0.0
73	2.156053731733733E-4	0.0	0.0	20.90822326080129	0.0
74	2.8747383089783107E-4	0.0	0.0	21.288946415596605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9135	0.0	73.25671	1
GCGTAAG	185	0.0	71.35135	1
CGTAAGG	515	0.0	68.34951	2
TAGCGGG	2115	0.0	66.38298	3
TAGGGTA	2405	0.0	66.19542	5
CGAGGGA	1145	0.0	65.67685	4
ATAGCGG	715	0.0	64.8951	2
AGGGTAA	2390	0.0	64.10042	6
TTGCGGG	2475	0.0	64.0	3
ATACGCG	25	1.2358339E-4	64.0	1
TAGTCCG	50	3.0013325E-10	64.0	1
TAGGGCG	465	0.0	63.655914	5
CGGGTAT	435	0.0	63.448277	6
TACGGGT	675	0.0	63.407406	4
TATAGCG	190	0.0	63.15789	1
GATTACG	95	0.0	63.15789	1
CGGGATC	830	0.0	62.6506	6
ACGGGTC	660	0.0	62.42424	5
ATAGGGT	1545	0.0	62.135918	4
CGCGAGG	400	0.0	62.0	2
>>END_MODULE