FastQCFastQC Report
Thu 26 May 2016
SRR1547268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547268_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1032110
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.4299929271104824No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10370.10047378670878103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT29950.072.12021
TACGGGA5000.072.04
TTAGGCG800.070.01
ACGGGAT5750.067.478265
CGAGGGA7250.067.310354
TCGTTAG303.7953105E-666.666671
CGGGATC3650.065.7534266
TAGGACG559.094947E-1265.4545441
GGGCGAT7450.064.966447
TTAGGGA16250.064.492314
AGGGATT23000.062.2608646
ACGTAGG1550.061.9354862
TAGGGAC8850.061.9209065
AGGGACC10150.061.8719256
TAGCGGG6700.061.4925353
GGCGATA2150.061.3953488
AGGGATC14500.061.241386
CGTATGG1050.060.9523772
TAAGGGA15750.060.6984144
TCTAGCG602.1827873E-1160.0000041
Produced by FastQC (version 0.11.5)