##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547268_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1032110
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.030431833816166	33.0	31.0	34.0	31.0	34.0
2	32.45057116005077	34.0	31.0	34.0	31.0	34.0
3	32.57516834445941	34.0	31.0	34.0	31.0	34.0
4	36.11786825047718	37.0	35.0	37.0	35.0	37.0
5	36.07204561529294	37.0	35.0	37.0	35.0	37.0
6	36.12850762031179	37.0	36.0	37.0	35.0	37.0
7	36.342675683793395	37.0	37.0	37.0	35.0	37.0
8	36.33861700787707	37.0	37.0	37.0	35.0	37.0
9	38.11835947718751	39.0	39.0	39.0	37.0	39.0
10-11	37.69381364389454	39.0	37.5	39.0	35.0	39.0
12-13	36.86114852099098	39.0	35.0	39.0	33.5	39.0
14-15	37.93442220305975	40.0	35.0	41.0	33.0	41.0
16-17	38.138986639021034	40.0	35.0	41.0	34.0	41.0
18-19	38.03119483388399	40.0	36.0	41.0	34.0	41.0
20-21	37.872825570917826	40.0	35.0	41.0	34.0	41.0
22-23	37.68723634108767	40.0	35.0	41.0	33.5	41.0
24-25	37.663234054509694	40.0	35.0	41.0	34.0	41.0
26-27	37.64620292410693	40.0	35.0	41.0	34.0	41.0
28-29	37.59322843495364	40.0	35.0	41.0	34.0	41.0
30-31	37.43878268789179	40.0	35.0	41.0	33.0	41.0
32-33	37.14990407999147	40.0	35.0	41.0	33.0	41.0
34-35	36.88158335836296	39.0	35.0	41.0	32.0	41.0
36-37	36.71528325469184	39.0	35.0	41.0	31.5	41.0
38-39	36.62264874867989	39.0	35.0	41.0	31.0	41.0
40-41	36.49612492854444	39.0	35.0	41.0	31.0	41.0
42-43	36.453393049190495	39.0	35.0	41.0	31.0	41.0
44-45	36.32947360261987	39.0	35.0	41.0	31.0	41.0
46-47	36.20979643642635	38.0	35.0	41.0	31.0	41.0
48-49	36.125091317785895	38.0	35.0	40.5	31.0	41.0
50-51	35.905391867145944	38.0	35.0	40.0	30.0	41.0
52-53	35.71767495712666	37.5	35.0	40.0	30.0	41.0
54-55	35.54002674133571	37.0	35.0	40.0	29.5	41.0
56-57	35.25329470695953	36.5	34.5	40.0	29.0	41.0
58-59	34.98032670936237	36.0	34.0	40.0	28.0	41.0
60-61	34.62855945587195	35.5	34.0	39.0	27.5	41.0
62-63	34.24979120442589	35.0	34.0	39.0	26.5	40.5
64-65	33.939308794605225	35.0	34.0	38.5	26.0	40.0
66-67	33.65009834223096	35.0	34.0	37.5	26.0	40.0
68-69	33.21155932991638	35.0	33.0	37.0	25.5	39.5
70-71	32.95726376064567	35.0	33.0	36.5	25.0	39.0
72-73	32.714056641249485	35.0	33.0	36.0	25.0	39.0
74-75	32.43785788336515	35.0	33.0	35.5	24.0	38.0
76-77	30.485130460900486	33.0	30.0	34.5	20.0	36.0
78-79	31.543211963841063	34.0	31.5	35.0	22.0	36.5
80-81	31.726619740143978	35.0	32.0	35.0	22.0	36.0
82-83	31.57791320692562	35.0	32.0	35.0	20.0	36.0
84-85	31.38628489211421	34.0	32.0	35.0	20.0	36.0
86	30.984026896357946	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	0.0
11	8.0
12	12.0
13	35.0
14	128.0
15	322.0
16	883.0
17	1901.0
18	3803.0
19	6367.0
20	7879.0
21	8051.0
22	7473.0
23	6557.0
24	6691.0
25	7417.0
26	8818.0
27	10514.0
28	12930.0
29	16399.0
30	20952.0
31	26281.0
32	33100.0
33	44649.0
34	120449.0
35	106184.0
36	89217.0
37	160725.0
38	225151.0
39	98957.0
40	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.70929455193729	23.134646500857468	29.436106616542812	13.71995233066243
2	30.15075912451192	24.56705196151573	31.317301450426793	13.964887463545553
3	29.692959083818586	24.97640755345845	31.94436639505479	13.386266967668176
4	27.02037573514451	27.597639786456867	30.61650405480036	14.765480423598259
5	24.7764288690159	32.30866864965943	28.922692348683764	13.992210132640901
6	23.507474978442218	40.01075466762264	25.78475162531125	10.697018728623888
7	88.54763542645648	3.449147862146477	6.275881446745017	1.7273352646520235
8	89.06880080611562	2.7705380240478243	6.189068994583911	1.971592175252638
9	84.4077666140237	5.62595072230673	7.225392642257124	2.7408900214124463
10-11	41.54925347104475	27.63678290104737	19.371481721909486	11.442481905998392
12-13	31.013457867862922	24.71311197449884	27.419557992849597	16.853872164788637
14-15	17.729166464814796	30.46821559717472	35.039821336873004	16.762796601137474
16-17	23.331234073887476	24.98667777659358	32.01092906763814	19.671159081880806
18-19	24.744552421737993	26.817587272674427	29.800554204493707	18.637306101093877
20-21	27.720349575142183	26.977066397961458	28.506942089505966	16.795641937390393
22-23	22.75837846741142	23.747226555309027	31.292788559358982	22.20160641792057
24-25	20.94985030665336	25.97625253122245	33.527627869122476	19.546269293001714
26-27	19.067541250448112	31.950034395558614	29.193351483853462	19.789072870139808
28-29	17.955353596031433	27.085049074226585	34.80234664909748	20.157250680644506
30-31	22.79829669318193	27.322233095309606	30.263101801164606	19.61636841034386
32-33	26.0115200899129	26.953231729176153	28.145546501826356	18.889701679084595
34-35	20.733061398494346	27.75862068965517	30.43609692765306	21.072220984197422
36-37	23.111635387700925	28.607222098419744	29.31247638333123	18.9686661305481
38-39	21.293854337231497	29.211130596544944	28.620108321787406	20.874906744436156
40-41	21.63301392293457	26.423733904331904	28.743786999447735	23.199465173285795
42-43	21.669928592882542	25.81968007286045	28.673300326515584	23.837091007741424
44-45	20.48381470967242	29.874722655530906	28.231196287217447	21.41026634757923
46-47	21.40847390297546	28.948319462072842	28.231632287256204	21.4115743476955
48-49	21.879789944870218	26.137620990010756	30.803887182567752	21.17870188255128
50-51	20.098487564310005	29.51608840143008	28.641084768096427	21.74433926616349
52-53	21.932061505072134	25.720029841780427	30.52029337958163	21.8276152735658
54-55	22.0392206257085	25.92703297129182	30.713635174545352	21.32011122845433
56-57	22.921103370764744	24.884120878588522	29.28156882502834	22.913206925618393
58-59	23.71224966331108	27.220257530689558	28.496187421883327	20.571305384116034
60-61	24.490025288002247	27.54643400412747	27.139985079109785	20.823555628760502
62-63	22.804933582660762	25.863376965633506	30.12348489986533	21.208204551840403
64-65	20.690720950286305	28.864462121285523	30.01337066785517	20.431446260573
66-67	20.411244925443995	28.143705612773832	26.939957950218485	24.50509151156369
68-69	19.76601331253452	30.40606136942768	26.369185455038707	23.4587398629991
70-71	20.48943426572749	28.526271424557457	29.12480258887134	21.859491720843707
72-73	22.70034201780818	29.264177267926865	27.277228202420282	20.75825251184467
74-75	21.982734398465283	30.522667157570417	25.920492970710484	21.57410547325382
76-77	24.6157386325101	27.58184689616417	26.77514993556888	21.02726453575685
78-79	20.257869800699538	28.710166552014805	29.195192372905986	21.836771274379668
80-81	18.80468167152726	27.93810737227621	29.775895980079643	23.481314976116888
82-83	19.431213727219	27.45966999641511	30.915987636976677	22.19312863938921
84-85	17.957775818468964	27.089118407921635	29.494094621697297	25.459011151912105
86	19.753223978064355	27.183149082946585	30.102217786863804	22.96140915212526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	403.0
2	1655.5
3	1874.0
4	1094.5
5	1353.5
6	1606.5
7	1696.0
8	1774.5
9	1846.5
10	1829.5
11	1821.5
12	1870.5
13	1969.5
14	2022.0
15	2040.5
16	2162.0
17	2214.0
18	2266.0
19	2472.5
20	2481.5
21	2387.0
22	3022.5
23	3703.5
24	4044.0
25	5211.5
26	6540.5
27	7586.5
28	9296.5
29	10417.0
30	11915.5
31	14693.0
32	16916.5
33	19123.0
34	21083.0
35	23083.5
36	24386.0
37	26166.0
38	29212.5
39	31989.0
40	36571.5
41	41751.0
42	48017.5
43	52177.0
44	52390.5
45	53518.0
46	56052.0
47	56647.0
48	55520.0
49	50176.0
50	44934.0
51	42192.5
52	37452.0
53	34551.5
54	33248.5
55	32443.5
56	29694.0
57	27348.0
58	25379.5
59	19786.5
60	14560.0
61	11111.0
62	8552.0
63	6783.5
64	5727.0
65	4980.0
66	3635.5
67	2754.0
68	2186.0
69	1792.0
70	1513.0
71	1344.0
72	1016.0
73	550.0
74	294.5
75	188.0
76	140.5
77	66.0
78	44.5
79	39.0
80	38.5
81	25.0
82	11.5
83	11.5
84	9.5
85	3.5
86	0.0
87	2.0
88	3.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1032110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.48755625388644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83408166763455	15.557711574753927
2	6.871269811900683	2.6780851399009253
3	2.05841518471716	1.203404451180891
4	1.045613320737307	0.8150579363072511
5	0.6422441896756083	0.6257884887517564
6	0.5006081412330509	0.5853377588059564
7	0.3721877800448473	0.5077121210443153
8	0.3318143635775101	0.5173000860851404
9	0.2590423519918616	0.454329216592241
>10	4.903449402791718	24.939756805256387
>50	2.477278971605672	34.76897115545666
>100	0.7029912576907411	16.81117617450619
>500	0.0	0.0
>1k	0.0010035563992730067	0.5353690913583645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4438	0.4299929271104824	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1037	0.10047378670878103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9377779500247066E-4	0.0	0.0	0.03768978112798054	0.0
2	1.9377779500247066E-4	0.0	0.0	0.12682756682911706	0.0
3	1.9377779500247066E-4	0.0	0.0	0.18883646122990766	0.0
4	1.9377779500247066E-4	0.0	0.0	0.30597513830890116	0.0
5	1.9377779500247066E-4	0.0	0.0	0.5697067173072637	0.0
6	1.9377779500247066E-4	0.0	0.0	0.7679414015947913	0.0
7	1.9377779500247066E-4	0.0	0.0	0.90038852447898	0.0
8	1.9377779500247066E-4	0.0	0.0	1.191927217060197	0.0
9	1.9377779500247066E-4	0.0	0.0	1.278739669221304	0.0
10	1.9377779500247066E-4	0.0	0.0	1.4484890176434682	0.0
11	1.9377779500247066E-4	0.0	0.0	1.718518374979411	0.0
12	1.9377779500247066E-4	0.0	0.0	1.9394250612822277	0.0
13	1.9377779500247066E-4	0.0	0.0	2.0172268459757197	0.0
14	1.9377779500247066E-4	0.0	0.0	2.0472624042011027	0.0
15	1.9377779500247066E-4	0.0	0.0	2.1007450756217847	0.0
16	1.9377779500247066E-4	0.0	0.0	2.216624197033262	0.0
17	1.9377779500247066E-4	0.0	0.0	2.3668019881601765	0.0
18	1.9377779500247066E-4	0.0	0.0	2.5454651151524548	0.0
19	1.9377779500247066E-4	0.0	0.0	2.6680295704915173	0.0
20	1.9377779500247066E-4	0.0	0.0	2.795438470705642	0.0
21	1.9377779500247066E-4	0.0	0.0	2.9490073732450997	0.0
22	1.9377779500247066E-4	0.0	0.0	3.1349371675499706	0.0
23	1.9377779500247066E-4	0.0	0.0	3.3195105172898236	0.0
24	1.9377779500247066E-4	0.0	0.0	3.450891862301499	0.0
25	1.9377779500247066E-4	0.0	0.0	3.566577205917974	0.0
26	1.9377779500247066E-4	0.0	0.0	3.6878821055895203	0.0
27	1.9377779500247066E-4	0.0	0.0	3.817035005958667	0.0
28	1.9377779500247066E-4	0.0	0.0	3.940471461375241	0.0
29	1.9377779500247066E-4	0.0	0.0	4.102954142484813	0.0
30	1.9377779500247066E-4	0.0	0.0	4.280648380502078	0.0
31	1.9377779500247066E-4	0.0	0.0	4.438383505634089	0.0
32	1.9377779500247066E-4	0.0	0.0	4.579841295985893	0.0
33	1.9377779500247066E-4	0.0	0.0	4.731859976165331	0.0
34	2.90666692503706E-4	0.0	0.0	4.895892879634923	0.0
35	2.90666692503706E-4	0.0	0.0	5.076590673474726	0.0
36	2.90666692503706E-4	0.0	0.0	5.234035131914234	0.0
37	2.90666692503706E-4	0.0	0.0	5.421612037476626	0.0
38	2.90666692503706E-4	0.0	0.0	5.618296499404133	0.0
39	2.90666692503706E-4	0.0	0.0	5.9048938582127874	0.0
40	2.90666692503706E-4	0.0	0.0	6.106422765015357	0.0
41	2.90666692503706E-4	0.0	0.0	6.30194456017285	0.0
42	2.90666692503706E-4	0.0	0.0	6.49804768871535	0.0
43	2.90666692503706E-4	0.0	0.0	6.679520593735164	0.0
44	2.90666692503706E-4	0.0	0.0	6.859540165292459	0.0
45	2.90666692503706E-4	0.0	0.0	7.045082404007325	0.0
46	2.90666692503706E-4	0.0	0.0	7.253974867019989	0.0
47	2.90666692503706E-4	0.0	0.0	7.465289552470183	0.0
48	2.90666692503706E-4	0.0	0.0	7.6717597930453145	0.0
49	2.90666692503706E-4	0.0	0.0	7.8866593677030545	0.0
50	2.90666692503706E-4	0.0	0.0	8.117061165960992	0.0
51	2.90666692503706E-4	0.0	0.0	8.333607851876254	0.0
52	2.90666692503706E-4	0.0	0.0	8.58735987443199	0.0
53	2.90666692503706E-4	0.0	0.0	8.805553671604772	0.0
54	2.90666692503706E-4	0.0	0.0	9.03295191404017	0.0
55	2.90666692503706E-4	0.0	0.0	9.253567933650483	0.0
56	2.90666692503706E-4	0.0	0.0	9.490655065836005	0.0
57	2.90666692503706E-4	0.0	0.0	9.72948619817655	0.0
58	2.90666692503706E-4	0.0	0.0	9.955140440456928	0.0
59	2.90666692503706E-4	0.0	0.0	10.181860460609819	0.0
60	2.90666692503706E-4	0.0	0.0	10.429508482622976	0.0
61	2.90666692503706E-4	0.0	0.0	10.690624061388805	0.0
62	2.90666692503706E-4	0.0	0.0	10.979449864839989	0.0
63	2.90666692503706E-4	0.0	0.0	11.27999922488882	0.0
64	2.90666692503706E-4	0.0	0.0	11.5529352491498	0.0
65	2.90666692503706E-4	0.0	0.0	11.797676604237921	0.0
66	2.90666692503706E-4	0.0	0.0	12.039511292401004	0.0
67	2.90666692503706E-4	0.0	0.0	12.284930869771634	0.0
68	2.90666692503706E-4	0.0	0.0	12.582089118407922	0.0
69	2.90666692503706E-4	0.0	0.0	12.962668707792774	0.0
70	3.875555900049413E-4	0.0	0.0	13.224074953251106	0.0
71	3.875555900049413E-4	0.0	0.0	13.489938087994497	0.0
72	3.875555900049413E-4	0.0	0.0	13.754929222660376	0.0
73	3.875555900049413E-4	0.0	0.0	14.013719467886174	0.0
74	3.875555900049413E-4	0.0	0.0	14.316981717065042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	2995	0.0	72.1202	1
TACGGGA	500	0.0	72.0	4
TTAGGCG	80	0.0	70.0	1
ACGGGAT	575	0.0	67.47826	5
CGAGGGA	725	0.0	67.31035	4
TCGTTAG	30	3.7953105E-6	66.66667	1
CGGGATC	365	0.0	65.753426	6
TAGGACG	55	9.094947E-12	65.454544	1
GGGCGAT	745	0.0	64.96644	7
TTAGGGA	1625	0.0	64.49231	4
AGGGATT	2300	0.0	62.260864	6
ACGTAGG	155	0.0	61.935486	2
TAGGGAC	885	0.0	61.920906	5
AGGGACC	1015	0.0	61.871925	6
TAGCGGG	670	0.0	61.492535	3
GGCGATA	215	0.0	61.395348	8
AGGGATC	1450	0.0	61.24138	6
CGTATGG	105	0.0	60.952377	2
TAAGGGA	1575	0.0	60.698414	4
TCTAGCG	60	2.1827873E-11	60.000004	1
>>END_MODULE