FastQCFastQC Report
Thu 26 May 2016
SRR1547267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547267_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2257276
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210200.9312108931295952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116750.074.620991
CTACGGG3550.069.859153
TAACGGG7100.069.295783
CACGTCG351.1819975E-768.571431
GCGATAT1450.066.2068949
TATAGCG1950.065.641031
GGCGATA4400.065.4545448
AAGGGAT34850.065.3084645
CTAGGGA17900.064.8044744
ATAACGG2800.064.285712
TAGGGAC27100.063.6162345
ACGGGAT14000.063.4285775
CGGGATT9900.063.0303046
TAAGGGA29350.062.5553674
TACGGGA8900.062.4719124
AGCGGGA17300.062.4277424
ACGGGTA1350.062.222225
CGTTAGG4250.062.1176452
AGGGATT39250.061.859876
ATAGGGA29550.061.7258874
Produced by FastQC (version 0.11.5)