##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547267_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2257276
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00082577407459	33.0	31.0	34.0	31.0	34.0
2	32.418091540423056	34.0	31.0	34.0	31.0	34.0
3	32.507165716553935	34.0	31.0	34.0	31.0	34.0
4	36.06349290029221	37.0	35.0	37.0	35.0	37.0
5	36.02974603017088	37.0	35.0	37.0	35.0	37.0
6	36.08939092959833	37.0	35.0	37.0	35.0	37.0
7	36.324720149419036	37.0	36.0	37.0	35.0	37.0
8	36.320688741651445	37.0	37.0	37.0	35.0	37.0
9	38.099853097273	39.0	39.0	39.0	37.0	39.0
10-11	37.650886732504134	39.0	37.5	39.0	35.0	39.0
12-13	36.96007444371003	39.0	35.0	39.0	33.5	39.0
14-15	38.09769164249299	40.0	36.0	41.0	33.0	41.0
16-17	38.260125257168376	40.0	36.0	41.0	34.0	41.0
18-19	38.088962980158385	40.0	36.0	41.0	34.0	41.0
20-21	37.86969205360798	40.0	35.0	41.0	34.0	41.0
22-23	37.67030305554128	40.0	35.0	41.0	33.0	41.0
24-25	37.626110409183454	40.0	35.0	41.0	33.0	41.0
26-27	37.58421167814658	40.0	35.0	41.0	33.5	41.0
28-29	37.4857170766889	40.0	35.0	41.0	33.0	41.0
30-31	37.309156257365075	40.0	35.0	41.0	33.0	41.0
32-33	36.90894135232023	39.5	35.0	41.0	31.5	41.0
34-35	36.53241982814684	39.0	35.0	41.0	31.0	41.0
36-37	36.286381018537384	39.0	35.0	41.0	30.0	41.0
38-39	36.18679372836995	39.0	35.0	41.0	30.0	41.0
40-41	36.07035559674581	39.0	35.0	41.0	30.0	41.0
42-43	36.01808839503897	39.0	35.0	41.0	30.0	41.0
44-45	35.87725980340906	39.0	35.0	41.0	29.0	41.0
46-47	35.742165114057826	38.0	35.0	41.0	29.0	41.0
48-49	35.64145345983388	38.0	35.0	40.5	28.0	41.0
50-51	35.41107844144889	38.0	35.0	40.0	27.5	41.0
52-53	35.22187650070262	37.5	35.0	40.0	27.0	41.0
54-55	35.01631036700873	37.0	34.5	40.0	26.5	41.0
56-57	34.744050793965826	36.5	34.0	40.0	26.0	41.0
58-59	34.49028630083339	36.0	34.0	40.0	26.0	41.0
60-61	34.11895842599664	35.5	34.0	39.0	25.5	41.0
62-63	33.73970684134328	35.0	33.5	39.0	24.0	40.5
64-65	33.39226926614202	35.0	33.0	38.5	23.0	40.0
66-67	33.076430839649205	35.0	33.0	38.0	22.0	40.0
68-69	32.62027018406256	35.0	33.0	37.0	20.5	39.5
70-71	32.31197492021357	35.0	33.0	36.5	19.5	39.0
72-73	32.04336642927139	35.0	32.5	36.0	19.0	39.0
74-75	31.733636028558315	35.0	32.0	36.0	18.5	38.0
76-77	29.77480622662005	33.0	29.0	34.5	14.0	36.0
78-79	30.793907346731196	34.0	30.5	35.0	15.5	37.0
80-81	30.937892397739574	34.5	31.0	35.0	15.0	36.0
82-83	30.74986355235248	34.5	31.0	35.0	13.5	36.0
84-85	30.51719063153996	34.0	31.0	35.0	11.0	36.0
86	30.07470375798086	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	13.0
10	15.0
11	20.0
12	42.0
13	116.0
14	436.0
15	1227.0
16	3049.0
17	7072.0
18	15602.0
19	23221.0
20	25426.0
21	23788.0
22	20548.0
23	18025.0
24	17122.0
25	18655.0
26	21579.0
27	25739.0
28	31510.0
29	39117.0
30	47819.0
31	59466.0
32	73281.0
33	94231.0
34	232077.0
35	221214.0
36	189949.0
37	334567.0
38	488531.0
39	223310.0
40	502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.71570512422938	23.76541459706301	29.636606245758163	14.88227403294945
2	32.34735140939788	24.197749854249103	30.9616103657683	12.493288370584722
3	28.329012491161915	24.065377915682443	34.866493951116304	12.739115642039343
4	25.953937400654592	26.754282595482344	33.72613716709875	13.565642836764313
5	24.408224780664835	30.949516142465523	31.222677244608104	13.41958183226154
6	21.63337580340198	40.47187849425591	27.50983929302398	10.384906409318134
7	86.52340254359679	3.47064337723876	8.3066935545321	1.6992605246323444
8	87.0266196955977	3.0528832096739613	8.221236570095993	1.6992605246323444
9	82.4996588808812	5.792955757293304	9.291198772325583	2.4161865894999104
10-11	38.33018204242636	28.25046649147025	21.58344836874179	11.835903097361598
12-13	28.649841667567454	25.87647677997728	29.181588782231326	16.292092770223935
14-15	17.268933883140562	31.50398976465439	34.936622725798706	16.29045362640634
16-17	21.928576744713542	26.973241198683723	32.67006338613444	18.4281186704683
18-19	23.59100083463431	28.39909696466006	30.601884749583125	17.408017451122504
20-21	25.48675926204859	28.512175737481815	30.077336577361386	15.923728423108209
22-23	21.12781068863533	25.452868856090262	32.29587343328862	21.123447021985793
24-25	19.588853999245107	28.30783652508599	33.75008195719088	18.353227518478025
26-27	19.018587004867815	33.76082499437375	29.058032779332255	18.16255522142618
28-29	17.418538982384078	28.686744554055423	34.68908985875011	19.205626604810398
30-31	21.823560787426967	29.196584733103087	30.691328840602566	18.288525638867377
32-33	24.677509529184736	29.029502816669293	28.255627579436453	18.037360074709515
34-35	19.994652847059907	29.602737990391958	29.67213579553409	20.730473367014046
36-37	22.8524336412561	29.579701374577144	29.319121808764194	18.248743175402566
38-39	20.44151889268304	30.940855260942833	28.56250188280033	20.055123963573795
40-41	20.72136947364877	28.47053262427811	28.810300556954488	21.99779734511863
42-43	21.12422229271033	27.942861218566094	28.720767863566525	22.212148625157045
44-45	20.153206785523793	31.395296809074303	27.6350787409249	20.816417664477008
46-47	20.881407501785336	30.43336304466091	28.086529959118867	20.598699494434886
48-49	21.323046007665877	27.534647956209163	30.28052395896647	20.861782077158487
50-51	19.574278909623814	30.912657557161815	28.75755999709384	20.75550353612053
52-53	21.71147879125105	28.08396049043183	29.61290511217946	20.591655606137664
54-55	21.753166205639012	28.466833475392466	29.66008587341557	20.119914445552958
56-57	21.68682518221077	27.394235352699447	29.20991938956512	21.70902007552466
58-59	22.950981625640818	28.540284838894316	28.250820900944323	20.257912634520547
60-61	23.057215865494516	30.24616839057342	26.93751229357863	19.759103450353436
62-63	21.85809356055706	27.964945358919337	29.705671792018347	20.47128928850526
64-65	19.650919958392326	30.979729550130337	29.54031762177067	19.829032869706673
66-67	19.271746122317342	30.234052016678508	26.17590848438561	24.318293376618545
68-69	19.064505182352534	32.004969706850204	25.948776312688395	22.98174879810887
70-71	19.488157407423813	30.248405600378508	28.3868698378045	21.87656715439317
72-73	21.544485477185777	30.684572910003034	27.032316827893442	20.73862478491775
74-75	21.026981193261257	31.96097863088076	25.68135221390738	21.3306879619506
76-77	24.067659426671796	29.387035524233635	26.016557124605054	20.52874792448952
78-79	19.621880532110385	30.387799276650263	28.793776215225787	21.196543976013565
80-81	17.879382051641006	29.68013215929288	29.6068801511202	22.833605637945915
82-83	19.506653151852056	28.49040170541839	29.824576170570193	22.17836897215936
84-85	18.049454298012293	28.73259627976375	28.682115080300324	24.53583434192363
86	18.737628894295604	28.536031925205425	29.964567912829448	22.761771267669527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	670.0
1	1087.5
2	6427.0
3	7320.5
4	3629.5
5	5043.5
6	7055.5
7	7991.0
8	8160.0
9	7944.0
10	7153.5
11	6522.5
12	6234.0
13	6338.0
14	6505.0
15	6572.5
16	6899.5
17	7052.5
18	7181.5
19	7528.0
20	8119.5
21	8600.0
22	9279.0
23	10309.5
24	11283.5
25	12672.5
26	17212.5
27	21140.0
28	23682.0
29	26070.0
30	30728.0
31	36658.0
32	40917.5
33	46384.5
34	53306.0
35	58332.5
36	58917.0
37	58708.5
38	64040.5
39	72245.5
40	81694.0
41	95436.0
42	104392.0
43	106390.0
44	112707.5
45	116561.5
46	120497.5
47	128032.5
48	123845.0
49	106063.5
50	93605.0
51	87772.0
52	76445.5
53	69035.5
54	63746.0
55	57966.0
56	53195.0
57	50831.0
58	43946.5
59	32454.0
60	24596.0
61	19315.5
62	15572.5
63	11622.5
64	9385.0
65	7802.0
66	5614.5
67	4529.5
68	3263.5
69	2128.0
70	1754.0
71	1730.0
72	1274.5
73	810.5
74	656.0
75	378.0
76	222.0
77	144.5
78	92.5
79	94.0
80	69.0
81	50.5
82	40.0
83	16.5
84	8.0
85	4.0
86	2.0
87	3.0
88	4.0
89	3.0
90	1.5
91	1.5
92	1.5
93	1.0
94	0.5
95	0.5
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2257276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.40143356813122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7228479588745	16.26460386495204
2	7.99883351643524	3.2637534121619
3	2.6725693526359686	1.6357273831207855
4	1.3905468579911464	1.134765973867199
5	0.8927580665655479	0.910677219372515
6	0.6199782855544428	0.7589067483853713
7	0.45625144700093356	0.6515728510447303
8	0.3882589077548395	0.6336830651036438
9	0.2991436143978368	0.5492662718821317
>10	3.1114891292025026	14.860101274210763
>50	1.152310091443757	17.017678430320295
>100	1.2912839075215878	40.76797664801013
>500	0.002851484710745636	0.4217194757320169
>1k	6.58034933248993E-4	0.18893247310489977
>5k	0.0	0.0
>10k+	2.193449777496643E-4	0.9406349087316421
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21020	0.9312108931295952	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.430118425925762E-5	0.0	0.0	0.042130426230554	0.0
2	4.430118425925762E-5	0.0	0.0	0.1402575493648096	0.0
3	4.430118425925762E-5	0.0	0.0	0.2113609501009181	0.0
4	4.430118425925762E-5	0.0	0.0	0.3226898261444325	0.0
5	8.860236851851524E-5	0.0	0.0	0.6079451515897923	0.0
6	8.860236851851524E-5	0.0	0.0	0.8661767546370049	0.0
7	8.860236851851524E-5	0.0	0.0	1.0424954679888503	0.0
8	8.860236851851524E-5	0.0	0.0	1.3759504819082824	0.0
9	8.860236851851524E-5	0.0	0.0	1.49126646453513	0.0
10	8.860236851851524E-5	0.0	0.0	1.695539225154567	0.0
11	8.860236851851524E-5	0.0	0.0	2.0342217788165913	0.0
12	8.860236851851524E-5	0.0	0.0	2.2606008303813976	0.0
13	8.860236851851524E-5	0.0	0.0	2.3439313579730614	0.0
14	8.860236851851524E-5	0.0	0.0	2.3820303764360227	0.0
15	8.860236851851524E-5	0.0	0.0	2.43948901242028	0.0
16	8.860236851851524E-5	0.0	0.0	2.569202879931386	0.0
17	8.860236851851524E-5	0.0	0.0	2.74264201630638	0.0
18	8.860236851851524E-5	0.0	0.0	2.9416429359989653	0.0
19	8.860236851851524E-5	0.0	0.0	3.0713125023258123	0.0
20	8.860236851851524E-5	0.0	0.0	3.207317138001733	0.0
21	8.860236851851524E-5	0.0	0.0	3.3795601424017265	0.0
22	8.860236851851524E-5	0.0	0.0	3.5707197524804233	0.0
23	8.860236851851524E-5	0.0	0.0	3.783232533372082	0.0
24	8.860236851851524E-5	0.0	0.0	3.939261304333187	0.0
25	8.860236851851524E-5	0.0	0.0	4.079031540671145	0.0
26	8.860236851851524E-5	0.0	0.0	4.210783262658177	0.0
27	8.860236851851524E-5	0.0	0.0	4.357154375450764	0.0
28	8.860236851851524E-5	0.0	0.0	4.516993048258166	0.0
29	8.860236851851524E-5	0.0	0.0	4.701994793724825	0.0
30	8.860236851851524E-5	0.0	0.0	4.906400457897041	0.0
31	8.860236851851524E-5	0.0	0.0	5.091003492705367	0.0
32	8.860236851851524E-5	0.0	0.0	5.260942835523879	0.0
33	8.860236851851524E-5	0.0	0.0	5.433761755319243	0.0
34	8.860236851851524E-5	0.0	0.0	5.6140232740701626	0.0
35	8.860236851851524E-5	0.0	0.0	5.8163024813979325	0.0
36	8.860236851851524E-5	0.0	0.0	5.998601854624778	0.0
37	8.860236851851524E-5	0.0	0.0	6.2077920466969925	0.0
38	8.860236851851524E-5	0.0	0.0	6.432753460365502	0.0
39	8.860236851851524E-5	0.0	0.0	6.722749012526603	0.0
40	8.860236851851524E-5	0.0	0.0	6.951165918567336	0.0
41	8.860236851851524E-5	0.0	0.0	7.181975088558067	0.0
42	8.860236851851524E-5	0.0	0.0	7.41211974078491	0.0
43	8.860236851851524E-5	0.0	0.0	7.6313220004997175	0.0
44	8.860236851851524E-5	0.0	0.0	7.841885529283969	0.0
45	8.860236851851524E-5	0.0	0.0	8.056790574125628	0.0
46	8.860236851851524E-5	0.0	0.0	8.292472874384877	0.0
47	8.860236851851524E-5	0.0	0.0	8.543704890319129	0.0
48	8.860236851851524E-5	0.0	0.0	8.787095596639489	0.0
49	8.860236851851524E-5	0.0	0.0	9.037928901915405	0.0
50	8.860236851851524E-5	0.0	0.0	9.295628890751507	0.0
51	8.860236851851524E-5	0.0	0.0	9.548765857608906	0.0
52	8.860236851851524E-5	0.0	0.0	9.823964814227415	0.0
53	8.860236851851524E-5	0.0	0.0	10.078076407138516	0.0
54	8.860236851851524E-5	0.0	0.0	10.328067989913507	0.0
55	8.860236851851524E-5	0.0	0.0	10.585236364538497	0.0
56	8.860236851851524E-5	0.0	0.0	10.851353578383858	0.0
57	1.3290355277777285E-4	0.0	0.0	11.117692298150514	0.0
58	1.3290355277777285E-4	0.0	0.0	11.3691015188218	0.0
59	1.3290355277777285E-4	0.0	0.0	11.62994689174031	0.0
60	1.3290355277777285E-4	0.0	0.0	11.905898968491226	0.0
61	1.3290355277777285E-4	0.0	0.0	12.201875180527326	0.0
62	1.3290355277777285E-4	0.0	0.0	12.506002810467129	0.0
63	1.3290355277777285E-4	0.0	0.0	12.827585107005081	0.0
64	1.3290355277777285E-4	0.0	0.0	13.11753635798192	0.0
65	1.3290355277777285E-4	0.0	0.0	13.413468268833762	0.0
66	1.7720473703703048E-4	0.0	0.0	13.680870217022642	0.0
67	1.7720473703703048E-4	0.0	0.0	13.963378869043927	0.0
68	2.2150592129628808E-4	0.0	0.0	14.278404590311508	0.0
69	2.658071055555457E-4	0.0	0.0	14.677425356934641	0.0
70	3.101082898148033E-4	0.0	0.0	14.973755978444816	0.0
71	3.9871065833331856E-4	0.0	0.0	15.271504237851286	0.0
72	3.9871065833331856E-4	0.0	0.0	15.56668302857072	0.0
73	3.9871065833331856E-4	0.0	0.0	15.86505150455682	0.0
74	3.9871065833331856E-4	0.0	0.0	16.186456596357733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11675	0.0	74.62099	1
CTACGGG	355	0.0	69.85915	3
TAACGGG	710	0.0	69.29578	3
CACGTCG	35	1.1819975E-7	68.57143	1
GCGATAT	145	0.0	66.206894	9
TATAGCG	195	0.0	65.64103	1
GGCGATA	440	0.0	65.454544	8
AAGGGAT	3485	0.0	65.308464	5
CTAGGGA	1790	0.0	64.804474	4
ATAACGG	280	0.0	64.28571	2
TAGGGAC	2710	0.0	63.616234	5
ACGGGAT	1400	0.0	63.428577	5
CGGGATT	990	0.0	63.030304	6
TAAGGGA	2935	0.0	62.555367	4
TACGGGA	890	0.0	62.471912	4
AGCGGGA	1730	0.0	62.427742	4
ACGGGTA	135	0.0	62.22222	5
CGTTAGG	425	0.0	62.117645	2
AGGGATT	3925	0.0	61.85987	6
ATAGGGA	2955	0.0	61.725887	4
>>END_MODULE