FastQCFastQC Report
Thu 26 May 2016
SRR1547266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547266_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3020991
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204060.6754737104479953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124500.073.12451
TACGGGA14750.066.440684
GCGGGAT21700.063.963135
ATAGCGG6900.063.1884042
AGGGATT53400.061.7977566
TAACGGG9200.061.7391323
TTGCGGG21650.061.524253
ATAGGGA39650.061.235814
TAGCGGG22300.061.165923
ACGGGAT14400.061.1111155
TAGGGAC31900.061.0658265
GGGCGAT18350.059.727527
TAGGGAT48600.059.6707845
TAAGGGA39050.059.4110154
TGCGGGA24250.059.2164964
CGTAAGG3800.058.9473722
CGGGATT14500.058.758626
TAGTAGG16600.058.313252
AGGGATC40500.058.2716066
GTAGGGA42850.058.1563574
Produced by FastQC (version 0.11.5)