##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547266_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3020991
Sequences flagged as poor quality	0
Sequence length	86
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99336343603804	33.0	31.0	33.0	31.0	34.0
2	32.425291237213216	33.0	31.0	34.0	31.0	34.0
3	32.54529424284945	34.0	31.0	34.0	31.0	34.0
4	36.09301649690449	37.0	35.0	37.0	35.0	37.0
5	36.06067181266015	37.0	35.0	37.0	35.0	37.0
6	36.113451182079	37.0	36.0	37.0	35.0	37.0
7	36.329626933678384	37.0	36.0	37.0	35.0	37.0
8	36.32508140540637	37.0	37.0	37.0	35.0	37.0
9	38.118960632454716	39.0	39.0	39.0	37.0	39.0
10-11	37.6641118758712	39.0	37.5	39.0	35.0	39.0
12-13	37.040605880653075	39.0	35.0	39.0	33.5	39.0
14-15	38.16970043935913	40.0	37.0	41.0	33.0	41.0
16-17	38.32692947446715	40.0	36.5	41.0	34.0	41.0
18-19	38.16875588176198	40.0	36.0	41.0	34.0	41.0
20-21	37.988720257690275	40.0	35.0	41.0	34.0	41.0
22-23	37.79994925506233	40.0	35.0	41.0	33.5	41.0
24-25	37.77450330040705	40.0	35.0	41.0	34.0	41.0
26-27	37.75925251018623	40.0	35.0	41.0	34.0	41.0
28-29	37.64871941028623	40.0	35.0	41.0	33.0	41.0
30-31	37.467643730153455	40.0	35.0	41.0	33.0	41.0
32-33	37.139068438138345	40.0	35.0	41.0	32.5	41.0
34-35	36.794288198806285	40.0	35.0	41.0	31.0	41.0
36-37	36.593548938080254	40.0	35.0	41.0	31.0	41.0
38-39	36.477860576214894	39.0	35.0	41.0	30.5	41.0
40-41	36.35256013672335	39.0	35.0	41.0	30.0	41.0
42-43	36.29877613008446	39.0	35.0	41.0	30.0	41.0
44-45	36.17771651752687	39.0	35.0	41.0	30.0	41.0
46-47	36.05049650925805	39.0	35.0	41.0	30.0	41.0
48-49	35.93677952036269	38.0	35.0	40.5	29.5	41.0
50-51	35.71540497803535	38.0	35.0	40.0	29.0	41.0
52-53	35.51952869108183	38.0	35.0	40.0	28.5	41.0
54-55	35.3228662382642	37.0	35.0	40.0	28.0	41.0
56-57	35.0323087688775	37.0	34.5	40.0	27.5	41.0
58-59	34.78752468974585	36.0	34.0	40.0	27.0	41.0
60-61	34.42957377231511	36.0	34.0	39.0	26.0	41.0
62-63	34.09185429549443	35.0	34.0	39.0	26.0	40.5
64-65	33.77343941110715	35.0	34.0	38.5	25.5	40.0
66-67	33.46681949731065	35.0	33.0	38.0	25.0	40.0
68-69	32.97596897839153	35.0	33.0	37.0	23.0	39.5
70-71	32.713471837552646	35.0	33.0	37.0	23.0	39.0
72-73	32.463990127742846	35.0	33.0	36.0	23.0	39.0
74-75	32.17330885792113	35.0	33.0	36.0	22.0	38.0
76-77	30.2620860174691	33.0	30.0	34.5	19.5	36.0
78-79	31.376113003977835	34.0	31.5	35.0	20.0	36.5
80-81	31.486370035528076	35.0	32.0	35.0	20.0	36.0
82-83	31.311979413377927	34.5	32.0	35.0	19.5	36.0
84-85	31.111682226130434	34.0	32.0	35.0	19.0	36.0
86	30.692626029008363	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	10.0
11	25.0
12	55.0
13	150.0
14	406.0
15	1322.0
16	3224.0
17	7375.0
18	16095.0
19	25139.0
20	29463.0
21	29694.0
22	25955.0
23	22433.0
24	21472.0
25	23156.0
26	26531.0
27	31639.0
28	38331.0
29	47597.0
30	58535.0
31	73550.0
32	92720.0
33	124314.0
34	294195.0
35	268863.0
36	279509.0
37	497664.0
38	692053.0
39	289005.0
40	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.554615356351604	23.220029453910985	29.589495632393476	14.635859557343931
2	31.5415040958414	24.779914935198416	30.384996181716527	13.293584787243656
3	29.22137139766388	23.821189801624698	33.566501853199824	13.390936947511594
4	26.155423832775405	26.967409038954436	32.596323524300466	14.28084360396969
5	24.739795649838083	31.71048837947548	30.09072188563289	13.458994085053547
6	22.47514143537667	40.43924659159858	26.570949731396087	10.514662241628656
7	88.01353595558543	3.1943491390739003	7.245734926055722	1.5463799792849433
8	88.57742376590993	2.453962954540414	7.290521554019856	1.6780917255298016
9	84.0714851517267	5.076744684111936	8.336966247168561	2.5148039169928014
10-11	38.027918653183676	27.206088995299886	21.648343209231673	13.117649142284767
12-13	29.334959951883338	22.554635217383964	29.05074857886038	19.05965625187232
14-15	19.188256436381305	28.305910212906955	34.04278595997141	18.46304739074032
16-17	25.54391919737596	25.66412809571429	30.62632427570953	18.165628431200226
18-19	25.40335274087212	26.843625154791923	29.20232135746184	18.55070074687412
20-21	27.20875368380773	26.957197158151086	29.419104525634136	16.414944632407046
22-23	23.650914550887443	22.098659016197004	33.40182079324301	20.84860563967254
24-25	21.727787338658075	26.371975288903542	32.07391216988068	19.826325202557705
26-27	20.629323291595377	30.356975575233424	29.11226812658495	19.90143300658625
28-29	19.3320337597828	27.642799995100948	32.619147160650265	20.406019084465992
30-31	23.25857640754309	25.89888549816931	30.785195983702035	20.057342110585566
32-33	25.17996577944125	26.55516021067259	29.167879017183434	19.096994992702726
34-35	21.34499904170519	27.410690730293467	30.11106289293811	21.133247335063228
36-37	23.576866001917914	28.470971942650607	28.45149158008084	19.50067047535064
38-39	22.520888013238043	27.849073366984545	27.88479012350583	21.74524849627159
40-41	22.234260214611695	26.441190986666296	28.327128415807923	22.997420382914083
42-43	21.601173257384744	26.591588654186655	28.737374589993813	23.069863498434785
44-45	21.821862428587174	27.88513769157207	28.069613580444297	22.223386299396456
46-47	21.549286310353125	27.62232658091335	28.835438437254528	21.992948671478995
48-49	22.306885389595664	26.39049570157607	29.786930844878384	21.515688063949874
50-51	20.70168365281459	28.233251936202393	28.827775388936942	22.237289022046077
52-53	22.03808286750937	26.164344746475578	29.544245580341023	22.253326805674032
54-55	22.30807705153706	26.066810526744373	30.062138549899686	21.562973871818883
56-57	22.164266626414975	25.889732872424975	29.02679948401038	22.91920101714967
58-59	23.167976998276394	27.39840999195297	27.96833886628593	21.465274143484706
60-61	23.625724141515153	27.689258259955096	27.13076603008748	21.554251568442275
62-63	22.535833440086385	26.014642215087697	29.215148274192142	22.234376070633775
64-65	21.641772517693695	27.914515468599543	29.106806342686887	21.336905671019874
66-67	21.34587623730094	28.288382851852255	26.989024462502538	23.37671644834427
68-69	20.822869051910452	28.908924256973954	27.102430957258726	23.16577573385687
70-71	21.1117974201181	28.204089320358783	28.23803513482827	22.446078124694843
72-73	22.218421041307305	28.448694484690616	27.543594138479726	21.78929033552235
74-75	21.227521035315895	29.498697612803216	26.4037860423947	22.869995309486193
76-77	22.884692473430075	27.469677996392573	26.870371345032147	22.775258185145205
78-79	20.89186958848934	28.160494354336045	28.004949369263265	22.94268668791135
80-81	19.733723139195053	28.01357236747809	28.429545139326795	23.82315935400006
82-83	20.34062663543188	27.705262941862458	28.771949337154595	23.182161085551066
84-85	19.673097337926528	27.3403992266114	28.109352196017795	24.877151239444277
86	20.23868988686163	27.597169273261656	28.65576229786848	23.508378542008234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	636.0
1	916.5
2	5536.5
3	6590.5
4	3584.0
5	4412.5
6	5205.5
7	5449.0
8	5681.0
9	6052.0
10	6411.5
11	6613.0
12	6690.5
13	6832.5
14	6878.0
15	7034.0
16	7140.0
17	7367.0
18	7699.0
19	7773.0
20	8293.5
21	8795.0
22	9543.5
23	11095.5
24	12328.0
25	14158.0
26	18834.0
27	24378.5
28	29140.5
29	31633.0
30	33841.5
31	38460.0
32	43560.0
33	49165.5
34	54031.5
35	60811.5
36	65641.0
37	69984.0
38	77731.0
39	84955.0
40	94448.5
41	110670.5
42	126808.5
43	132398.0
44	136539.0
45	143275.0
46	149082.5
47	155053.0
48	155258.5
49	145310.5
50	137915.0
51	134326.5
52	124392.0
53	118278.5
54	114124.5
55	109719.5
56	102461.0
57	95221.0
58	84762.0
59	66325.5
60	50451.0
61	38674.0
62	30359.5
63	22740.5
64	19556.0
65	16668.5
66	12290.0
67	9320.0
68	7335.0
69	5919.5
70	4462.0
71	3914.0
72	3310.0
73	2247.5
74	1457.0
75	935.0
76	682.0
77	473.0
78	254.0
79	181.0
80	146.0
81	85.0
82	52.0
83	44.0
84	32.5
85	17.0
86	12.0
87	14.5
88	10.5
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3020991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.887937449908247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84158039710614	14.28201196045255
2	7.489970805924003	2.6796025855601484
3	2.3183459785692877	1.2441128355568123
4	1.1815743897982507	0.8454371510849844
5	0.7236705971878861	0.6472487188417326
6	0.5276933830705911	0.5663607737458324
7	0.39747637449627105	0.4977022767363921
8	0.3246021438475567	0.46451702762009706
9	0.270442015070295	0.43538848644641226
>10	3.6932621619968304	16.797640633989197
>50	1.7707006724120213	22.887832697847703
>100	1.4588048074435735	37.711652121139196
>500	0.001313391154221529	0.16798109051993915
>1k	3.7525461549186537E-4	0.08763209337613609
>5k	0.0	0.0
>10k+	1.8762730774593268E-4	0.6848795470828928
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20406	0.6754737104479953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.324068823773391E-4	0.0	0.0	0.02310500097484567	0.0
2	1.324068823773391E-4	0.0	0.0	0.06600483086510353	0.0
3	1.324068823773391E-4	0.0	0.0	0.09943756866538166	0.0
4	1.324068823773391E-4	0.0	0.0	0.1713345057962768	0.0
5	1.324068823773391E-4	0.0	0.0	0.33710792253270533	0.0
6	1.324068823773391E-4	0.0	0.0	0.4946721125617388	0.0
7	1.324068823773391E-4	0.0	0.0	0.621451702438041	0.0
8	1.324068823773391E-4	0.0	0.0	0.8759046286466924	0.0
9	1.324068823773391E-4	0.0	3.310172059433477E-5	0.9869609012406856	0.0
10	1.324068823773391E-4	0.0	3.310172059433477E-5	1.1664053285825744	0.0
11	1.324068823773391E-4	0.0	3.310172059433477E-5	1.3856380240788535	0.0
12	1.324068823773391E-4	0.0	3.310172059433477E-5	1.559322752037328	0.0
13	1.324068823773391E-4	0.0	3.310172059433477E-5	1.6259565155937241	0.0
14	1.324068823773391E-4	0.0	3.310172059433477E-5	1.657800370805474	0.0
15	1.324068823773391E-4	0.0	3.310172059433477E-5	1.699210623268987	0.0
16	1.324068823773391E-4	0.0	3.310172059433477E-5	1.7753114789153626	0.0
17	1.324068823773391E-4	0.0	3.310172059433477E-5	1.8778275075960174	0.0
18	1.324068823773391E-4	0.0	3.310172059433477E-5	2.004938114678263	0.0
19	1.324068823773391E-4	0.0	3.310172059433477E-5	2.102753699034522	0.0
20	1.324068823773391E-4	0.0	3.310172059433477E-5	2.216226397231902	0.0
21	1.324068823773391E-4	0.0	3.310172059433477E-5	2.3504538742419294	0.0
22	1.324068823773391E-4	0.0	3.310172059433477E-5	2.5037810440348878	0.0
23	1.6550860297167387E-4	0.0	3.310172059433477E-5	2.649759631855904	0.0
24	1.6550860297167387E-4	0.0	3.310172059433477E-5	2.7611469216558406	0.0
25	1.6550860297167387E-4	0.0	3.310172059433477E-5	2.86270300043926	0.0
26	1.6550860297167387E-4	0.0	3.310172059433477E-5	2.9662782841789332	0.0
27	1.9861032356600865E-4	0.0	3.310172059433477E-5	3.077202149890549	0.0
28	1.9861032356600865E-4	0.0	3.310172059433477E-5	3.1918665100293246	0.0
29	1.9861032356600865E-4	0.0	3.310172059433477E-5	3.3527077703971977	0.0
30	2.317120441603434E-4	0.0	3.310172059433477E-5	3.5224202918843517	0.0
31	2.317120441603434E-4	0.0	3.310172059433477E-5	3.671344932838264	0.0
32	2.317120441603434E-4	0.0	3.310172059433477E-5	3.8141457554822242	0.0
33	2.317120441603434E-4	0.0	3.310172059433477E-5	3.9575755108174766	0.0
34	2.317120441603434E-4	0.0	3.310172059433477E-5	4.113815632022737	0.0
35	2.317120441603434E-4	0.0	3.310172059433477E-5	4.276278876699732	0.0
36	2.317120441603434E-4	0.0	3.310172059433477E-5	4.432221082419643	0.0
37	2.317120441603434E-4	0.0	3.310172059433477E-5	4.599914398950543	0.0
38	2.317120441603434E-4	0.0	3.310172059433477E-5	4.807230475032862	0.0
39	2.317120441603434E-4	0.0	3.310172059433477E-5	5.212958264357623	0.0
40	2.317120441603434E-4	0.0	3.310172059433477E-5	5.4212342903371775	0.0
41	2.317120441603434E-4	0.0	3.310172059433477E-5	5.632224657405467	0.0
42	2.317120441603434E-4	0.0	3.310172059433477E-5	5.820738956190204	0.0
43	2.317120441603434E-4	0.0	3.310172059433477E-5	6.000911621385168	0.0
44	2.317120441603434E-4	0.0	3.310172059433477E-5	6.1776748093589156	0.0
45	2.648137647546782E-4	0.0	3.310172059433477E-5	6.347983161816768	0.0
46	2.648137647546782E-4	0.0	3.310172059433477E-5	6.533419000586232	0.0
47	2.648137647546782E-4	0.0	3.310172059433477E-5	6.747554031110983	0.0
48	2.648137647546782E-4	0.0	3.310172059433477E-5	6.942456961970426	0.0
49	2.648137647546782E-4	0.0	3.310172059433477E-5	7.132063617534776	0.0
50	2.648137647546782E-4	0.0	3.310172059433477E-5	7.330276720453653	0.0
51	2.648137647546782E-4	0.0	3.310172059433477E-5	7.543683513125329	0.0
52	2.648137647546782E-4	0.0	3.310172059433477E-5	7.812999111880837	0.0
53	2.97915485349013E-4	0.0	3.310172059433477E-5	8.027200345846778	0.0
54	2.97915485349013E-4	0.0	3.310172059433477E-5	8.234648828811473	0.0
55	2.97915485349013E-4	0.0	3.310172059433477E-5	8.440872548114179	0.0
56	3.3101720594334774E-4	0.0	3.310172059433477E-5	8.648652048284818	0.0
57	3.3101720594334774E-4	0.0	3.310172059433477E-5	8.863151197736107	0.0
58	3.641189265376825E-4	0.0	3.310172059433477E-5	9.071559630598038	0.0
59	3.641189265376825E-4	0.0	3.310172059433477E-5	9.284900219828527	0.0
60	3.972206471320173E-4	0.0	3.310172059433477E-5	9.525682135431717	0.0
61	4.3032236772635204E-4	0.0	3.310172059433477E-5	9.783048013052671	0.0
62	4.3032236772635204E-4	0.0	3.310172059433477E-5	10.063883010575006	0.0
63	4.3032236772635204E-4	0.0	3.310172059433477E-5	10.333926847183589	0.0
64	4.3032236772635204E-4	0.0	3.310172059433477E-5	10.59440428654041	0.0
65	4.3032236772635204E-4	0.0	3.310172059433477E-5	10.834921388378847	0.0
66	4.3032236772635204E-4	0.0	3.310172059433477E-5	11.06411770177402	0.0
67	4.3032236772635204E-4	0.0	3.310172059433477E-5	11.29976885068509	0.0
68	4.634240883206868E-4	0.0	3.310172059433477E-5	11.575936505603625	0.0
69	4.965258089150216E-4	0.0	3.310172059433477E-5	12.027377771069162	0.0
70	5.296275295093564E-4	0.0	3.310172059433477E-5	12.304737087929093	0.0
71	5.296275295093564E-4	0.0	3.310172059433477E-5	12.562169168991234	0.0
72	5.296275295093564E-4	0.0	3.310172059433477E-5	12.81268299044916	0.0
73	5.627292501036911E-4	0.0	3.310172059433477E-5	13.06789725623148	0.0
74	5.627292501036911E-4	0.0	3.310172059433477E-5	13.352605155063355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12450	0.0	73.1245	1
TACGGGA	1475	0.0	66.44068	4
GCGGGAT	2170	0.0	63.96313	5
ATAGCGG	690	0.0	63.188404	2
AGGGATT	5340	0.0	61.797756	6
TAACGGG	920	0.0	61.739132	3
TTGCGGG	2165	0.0	61.52425	3
ATAGGGA	3965	0.0	61.23581	4
TAGCGGG	2230	0.0	61.16592	3
ACGGGAT	1440	0.0	61.111115	5
TAGGGAC	3190	0.0	61.065826	5
GGGCGAT	1835	0.0	59.72752	7
TAGGGAT	4860	0.0	59.670784	5
TAAGGGA	3905	0.0	59.411015	4
TGCGGGA	2425	0.0	59.216496	4
CGTAAGG	380	0.0	58.947372	2
CGGGATT	1450	0.0	58.75862	6
TAGTAGG	1660	0.0	58.31325	2
AGGGATC	4050	0.0	58.271606	6
GTAGGGA	4285	0.0	58.156357	4
>>END_MODULE