FastQCFastQC Report
Thu 26 May 2016
SRR1547265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547265_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4106347
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670231.632180621851977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT423900.076.7445141
CGTAAGG7150.064.335662
CGAGGGA22350.062.281884
CGGGATA10350.062.2222256
TAGGGAT60050.061.548715
ACGGGAT19400.061.030935
GGCGATA7350.059.8639458
ATAGGGA58400.059.246584
TACGGGA19800.058.5858544
TAGGGAC41150.058.3232045
CGTAGGG19550.058.3120163
ACGGGAC12900.057.9844935
TAGCGGG29150.057.9073753
ATAGCGG10000.057.62
GGGCGAT32700.057.492357
ACGGGTA5650.057.345135
GCGGGAT26800.057.164185
GCGGGTC9100.057.1428575
GTAGGGA53700.057.0577284
TAGGGTC21050.057.0071225
Produced by FastQC (version 0.11.5)