##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547265_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4106347
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05559150261778	33.0	31.0	34.0	31.0	34.0
2	32.49307425797187	34.0	31.0	34.0	31.0	34.0
3	32.51887675347456	34.0	31.0	34.0	31.0	34.0
4	36.11194353521512	37.0	35.0	37.0	35.0	37.0
5	36.096389077688755	37.0	35.0	37.0	35.0	37.0
6	36.15159069606149	37.0	36.0	37.0	35.0	37.0
7	36.36935261437964	37.0	37.0	37.0	35.0	37.0
8	36.367968902774166	37.0	37.0	37.0	35.0	37.0
9	38.15992973803724	39.0	39.0	39.0	37.0	39.0
10-11	37.71444120528538	39.0	37.5	39.0	35.0	39.0
12-13	37.1959912301615	39.0	37.0	39.0	34.5	39.0
14-15	38.39143866799372	40.0	37.0	41.0	33.5	41.0
16-17	38.52112437161302	40.0	37.0	41.0	34.0	41.0
18-19	38.19619079926757	40.0	37.0	41.0	34.0	41.0
20-21	37.85324523231963	40.0	35.0	41.0	34.0	41.0
22-23	37.68580261239491	39.5	35.0	41.0	33.5	41.0
24-25	37.64194246126789	40.0	35.0	41.0	34.0	41.0
26-27	37.61268531373506	40.0	35.0	41.0	34.0	41.0
28-29	37.47579612731218	40.0	35.0	41.0	33.0	41.0
30-31	37.15633274538172	40.0	35.0	41.0	32.5	41.0
32-33	36.48198423075303	40.0	35.0	41.0	30.5	41.0
34-35	35.767331523614544	39.0	35.0	41.0	25.5	41.0
36-37	35.470270169569204	39.0	35.0	41.0	23.5	41.0
38-39	35.32068380972188	39.0	35.0	41.0	23.0	41.0
40-41	35.14706380147611	39.0	35.0	41.0	22.5	41.0
42-43	35.06629931664324	39.0	35.0	41.0	22.0	41.0
44-45	34.92783500761139	38.5	35.0	41.0	22.0	41.0
46-47	34.80718555933047	38.0	35.0	41.0	22.0	41.0
48-49	34.67803378525974	38.0	34.5	40.0	22.0	41.0
50-51	34.446107087394225	37.5	34.0	40.0	20.0	41.0
52-53	34.23438874016249	37.0	34.0	40.0	20.0	41.0
54-55	34.01290697059942	37.0	34.0	40.0	20.0	41.0
56-57	33.73842164337305	36.0	34.0	40.0	19.0	41.0
58-59	33.49159155327107	36.0	33.5	40.0	18.0	41.0
60-61	33.14086741816997	35.0	33.0	39.0	18.0	41.0
62-63	32.80426118396716	35.0	33.0	39.0	17.0	40.5
64-65	32.47833828948211	35.0	32.5	38.5	15.0	40.0
66-67	32.14957747116841	35.0	32.0	38.0	13.0	40.0
68-69	31.628311976557264	35.0	31.5	37.0	10.0	39.5
70-71	31.335291927350514	35.0	31.0	36.5	10.0	39.0
72-73	31.118083907667813	35.0	31.0	36.0	10.0	39.0
74-75	30.848084928039448	35.0	31.0	36.0	10.0	38.0
76-77	28.968550514605802	33.0	28.0	34.5	10.0	36.0
78-79	30.04730908030909	34.0	30.0	35.0	10.0	36.5
80-81	30.143886037882332	34.0	30.5	35.0	10.0	36.0
82-83	29.979896121784158	34.0	30.0	35.0	10.0	36.0
84-85	29.767213170245963	34.0	30.0	35.0	10.0	36.0
86	29.363703554521816	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	15.0
11	37.0
12	76.0
13	228.0
14	828.0
15	2772.0
16	7308.0
17	17956.0
18	42976.0
19	73057.0
20	85626.0
21	79142.0
22	63297.0
23	47429.0
24	38187.0
25	36122.0
26	38732.0
27	44033.0
28	52961.0
29	66150.0
30	82082.0
31	99798.0
32	113996.0
33	150398.0
34	361197.0
35	333122.0
36	337926.0
37	624989.0
38	908558.0
39	396530.0
40	796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.667475008809536	22.00851511087592	28.80567570154203	20.51833417877252
2	36.29252471844197	23.570901338829863	28.451346172157393	11.685227770570778
3	26.33793490905664	23.08087942884515	38.3560132643442	12.225172397754013
4	24.752340705741624	25.271293439156505	37.082716097787156	12.893649757314712
5	22.728814686143185	29.811362751370012	34.89485910469817	12.56496345778864
6	21.18028505627995	37.42277503581651	31.196900797716314	10.20003911018723
7	82.5887583294836	2.849734812961496	13.177381258817144	1.384125598737759
8	83.35849357104989	2.355134624521503	12.89189637407652	1.3944754303520868
9	78.93994345826107	4.543795251594665	14.251572017659491	2.2646892724847656
10-11	34.07960895657381	24.9771634009498	28.444868395194074	12.498359247282318
12-13	27.795252081716427	21.08197383221632	33.22677065528071	17.896003430786536
14-15	17.99967221474464	26.48895721671841	38.3520316232408	17.15933894529615
16-17	23.756772138350705	24.46610089210678	35.08085166694388	16.696275302598636
18-19	23.1605487797305	26.156374509996354	33.912927962493185	16.770148747779963
20-21	24.95775442260481	25.995574655527165	34.0591528187949	14.987518103073121
22-23	21.474086335129495	22.335862020428376	37.45040299809052	18.73964864635161
24-25	20.673070249542963	25.541131813750763	35.8830488509617	17.902749085744578
26-27	19.030564148621636	28.701105873419852	33.870274479969666	18.398055497988842
28-29	18.304480843922835	26.60626342586245	36.41837867087219	18.670877059342526
30-31	21.662282802695437	25.357489272095123	34.633799822567354	18.346428102642083
32-33	24.161328791746044	25.759038386186067	32.27101849892374	17.80861432314415
34-35	20.894361825729778	27.231697662180032	32.761174347905815	19.11276616418437
36-37	22.35556322931306	29.31795583763379	30.201052176058184	18.125428756994964
38-39	21.87953185641642	28.542680392085718	29.688272812794438	19.889514938703424
40-41	21.383385281370522	27.21957009478254	30.40137621102162	20.995668412825317
42-43	21.01047476017005	27.25208074232402	30.253373618936735	21.4840708785692
44-45	20.934056474038847	28.342685116479437	29.521092591541827	21.202165817939886
46-47	20.733135801723527	28.451723636604502	30.042115291279575	20.773025270392395
48-49	21.444851104887142	26.289071527564523	31.609725140130635	20.656352227417702
50-51	19.630927439887568	28.140741637275173	30.789300076199112	21.439030846638143
52-53	21.23344666196013	26.20566406102553	31.772899367734876	20.787989909279464
54-55	20.826259933707505	26.045497372725684	32.25176781212109	20.876474881445723
56-57	21.234213767126842	26.73014482214971	30.53244160807647	21.503199802646975
58-59	22.127550350713175	27.878976131340096	29.646666489704838	20.34680702824189
60-61	23.188432443726747	28.09889178873583	28.691657085969595	20.021018681567824
62-63	21.8562751759654	27.141605422045433	30.264514908262747	20.73760449372642
64-65	21.226031312015277	29.371555789123523	29.461246212266033	19.941166686595167
66-67	21.544099901932302	29.161515088715106	27.557534713944047	21.736850295408548
68-69	20.56378820396815	29.84580942623699	28.219899584716053	21.370502785078806
70-71	20.8294622933717	29.259899370413656	29.214092233315892	20.696546102898754
72-73	21.53739077579172	30.170976783014197	28.031350005247972	20.26028243594611
74-75	20.86133977474383	29.98162356956195	27.799002373642555	21.358034282051662
76-77	22.084190644385387	28.23529039314018	28.01564261373917	21.664876348735262
78-79	20.656729691864815	29.297998927026867	28.502888333596747	21.54238304751157
80-81	19.80697198751104	28.800732134912128	28.87856286865187	22.513733008924962
82-83	20.428022765733147	29.2109751075591	28.83821070162848	21.522791425079273
84-85	19.980008995830115	28.425447240576602	28.31495974402553	23.279584019567757
86	20.172162751954474	29.208953846326185	28.59039920396401	22.02848419775533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1010.0
1	1367.5
2	15606.0
3	19567.0
4	10369.5
5	12257.0
6	14345.0
7	15268.0
8	15891.0
9	17003.5
10	17932.5
11	18586.5
12	19001.5
13	19255.5
14	19309.0
15	19832.0
16	20651.5
17	21062.0
18	21477.0
19	21998.0
20	22301.5
21	22385.0
22	23784.5
23	26035.0
24	27451.5
25	29207.5
26	33047.0
27	38102.0
28	44288.0
29	48068.0
30	52155.0
31	60136.5
32	68142.5
33	76309.5
34	84129.5
35	92071.0
36	96248.0
37	105026.0
38	114887.5
39	122861.0
40	137516.0
41	152127.0
42	164586.5
43	170200.0
44	174594.5
45	180313.5
46	184679.0
47	189670.0
48	191368.5
49	181273.5
50	171430.0
51	163506.0
52	150830.5
53	142914.0
54	134720.5
55	124461.0
56	114526.0
57	109822.0
58	98182.0
59	75128.0
60	56521.0
61	45968.5
62	38705.5
63	29507.5
64	24213.0
65	20373.0
66	13788.0
67	9676.5
68	7554.0
69	5641.0
70	3728.0
71	2972.0
72	2744.5
73	1975.0
74	1255.5
75	755.0
76	387.5
77	267.5
78	169.5
79	147.0
80	106.0
81	57.0
82	40.0
83	27.5
84	16.0
85	8.5
86	9.0
87	7.0
88	6.0
89	8.5
90	6.5
91	2.5
92	1.0
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	4106347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.55900440952258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00359363755206	16.447942343720854
2	7.018918450318473	2.886039507403538
3	2.3432376076794688	1.44523896926524
4	1.269880189137835	1.0442988963220048
5	0.8280386865696752	0.8511825504220619
6	0.6050982926565307	0.7464131080152121
7	0.464624151357441	0.6686546983569821
8	0.3755465826538374	0.6176691079009102
9	0.31414746112422576	0.5812703134643953
>10	4.160114219270801	21.007820223817298
>50	1.6857404832526452	24.997377077967414
>100	0.9264988518020526	26.016108351104243
>500	0.0025207662927762475	0.3564781489386113
>1k	0.0019205838421151762	0.6794925131793346
>5k	0.0	0.0
>10k+	1.2003649013219851E-4	1.654014190121978
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67023	1.632180621851977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.305763492466662E-5	0.0	0.0	0.01534210333417999	0.0
2	7.305763492466662E-5	0.0	0.0	0.05605955853219419	0.0
3	7.305763492466662E-5	0.0	0.0	0.10917245912242682	0.0
4	7.305763492466662E-5	0.0	0.0	0.18291196530639034	0.0
5	7.305763492466662E-5	0.0	0.0	0.3773913894758529	0.0
6	7.305763492466662E-5	0.0	0.0	0.6476072285172199	0.0
7	7.305763492466662E-5	0.0	0.0	0.8694345606934826	0.0
8	7.305763492466662E-5	0.0	0.0	1.1457385359785717	0.0
9	9.741017989955549E-5	0.0	0.0	1.3529056360799514	0.0
10	9.741017989955549E-5	0.0	0.0	1.6209053935286033	0.0
11	9.741017989955549E-5	0.0	0.0	1.927601344942354	0.0
12	9.741017989955549E-5	0.0	0.0	2.13659488591685	0.0
13	1.2176272487444437E-4	0.0	0.0	2.2257008479799687	0.0
14	1.2176272487444437E-4	0.0	0.0	2.2763784940727123	0.0
15	1.2176272487444437E-4	0.0	0.0	2.332243232245107	0.0
16	1.4611526984933325E-4	0.0	0.0	2.4231269300913927	0.0
17	1.704678148242221E-4	0.0	0.0	2.5285490972876867	0.0
18	1.704678148242221E-4	0.0	0.0	2.6570331245752	0.0
19	1.704678148242221E-4	0.0	0.0	2.7501085514692254	0.0
20	1.704678148242221E-4	0.0	0.0	2.857430217173561	0.0
21	1.704678148242221E-4	0.0	0.0	2.9772447384500142	0.0
22	1.704678148242221E-4	0.0	0.0	3.11505579046291	0.0
23	1.704678148242221E-4	0.0	0.0	3.255983968232592	0.0
24	1.704678148242221E-4	0.0	0.0	3.365132074810044	0.0
25	1.704678148242221E-4	0.0	0.0	3.467071828074929	0.0
26	1.704678148242221E-4	0.0	0.0	3.5667224421121744	0.0
27	1.704678148242221E-4	0.0	0.0	3.688874807706217	0.0
28	1.704678148242221E-4	0.0	0.0	3.8035022369030185	0.0
29	1.704678148242221E-4	0.0	0.0	3.942189980535011	0.0
30	1.704678148242221E-4	0.0	0.0	4.0813647750665005	0.0
31	1.9482035979911098E-4	0.0	0.0	4.206609913872354	0.0
32	1.9482035979911098E-4	0.0	0.0	4.324938929905339	0.0
33	1.9482035979911098E-4	0.0	0.0	4.447627051488829	0.0
34	1.9482035979911098E-4	0.0	0.0	4.5834655473587596	0.0
35	1.9482035979911098E-4	0.0	0.0	4.7308715020917615	0.0
36	1.9482035979911098E-4	0.0	0.0	4.8725546087556655	0.0
37	1.9482035979911098E-4	0.0	0.0	5.024490136853997	0.0
38	2.1917290477399987E-4	0.0	0.0	5.208546671774207	0.0
39	2.1917290477399987E-4	0.0	0.0	5.4948108379540255	0.0
40	2.1917290477399987E-4	0.0	0.0	5.690434831737308	0.0
41	2.1917290477399987E-4	0.0	0.0	5.905078163145979	0.0
42	2.1917290477399987E-4	0.0	0.0	6.10084827219911	0.0
43	2.1917290477399987E-4	0.0	0.0	6.265349713504484	0.0
44	2.4352544974888874E-4	0.0	0.0	6.420767655534226	0.0
45	2.678779947237776E-4	0.0	0.0	6.577695455352409	0.0
46	2.678779947237776E-4	0.0	0.0	6.753082484261559	0.0
47	2.678779947237776E-4	0.0	0.0	6.939696036404133	0.0
48	3.1658308467355533E-4	0.0	0.0	7.117177384181122	0.0
49	3.1658308467355533E-4	0.0	0.0	7.295657186302083	0.0
50	3.1658308467355533E-4	0.0	0.0	7.4780333956190255	0.0
51	3.1658308467355533E-4	0.0	0.0	7.671587423079442	0.0
52	3.1658308467355533E-4	0.0	0.0	7.887350971556958	0.0
53	3.1658308467355533E-4	0.0	0.0	8.076813771461593	0.0
54	3.1658308467355533E-4	0.0	0.0	8.266520096815977	0.0
55	3.409356296484442E-4	0.0	0.0	8.44928594685252	0.0
56	3.652881746233331E-4	0.0	0.0	8.63891921457198	0.0
57	3.652881746233331E-4	0.0	0.0	8.835273784704507	0.0
58	3.652881746233331E-4	0.0	0.0	9.021327228312659	0.0
59	3.652881746233331E-4	0.0	0.0	9.21824190697961	0.0
60	3.8964071959822196E-4	0.0	0.0	9.423095515308376	0.0
61	3.8964071959822196E-4	0.0	0.0	9.655053506194191	0.0
62	4.1399326457311085E-4	0.0	0.0	9.947016167898134	0.0
63	4.1399326457311085E-4	0.0	0.0	10.197409035329942	0.0
64	4.1399326457311085E-4	0.0	0.0	10.42439910704088	0.0
65	4.1399326457311085E-4	0.0	0.0	10.639699957163874	0.0
66	4.1399326457311085E-4	0.0	0.0	10.848157742148922	0.0
67	4.1399326457311085E-4	0.0	0.0	11.06238708029302	0.0
68	4.3834580954799974E-4	0.0	0.0	11.309808937237891	0.0
69	4.3834580954799974E-4	0.0	0.0	11.6584399710984	0.0
70	4.8705089949777747E-4	0.0	0.0	11.918232920890514	0.0
71	4.8705089949777747E-4	0.0	0.0	12.159469231411764	0.0
72	5.114034444726664E-4	0.0	0.0	12.3868489438423	0.0
73	5.114034444726664E-4	0.0	0.0	12.611817754320324	0.0
74	5.357559894475552E-4	0.0	0.0	12.854953563349614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	42390	0.0	76.744514	1
CGTAAGG	715	0.0	64.33566	2
CGAGGGA	2235	0.0	62.28188	4
CGGGATA	1035	0.0	62.222225	6
TAGGGAT	6005	0.0	61.54871	5
ACGGGAT	1940	0.0	61.03093	5
GGCGATA	735	0.0	59.863945	8
ATAGGGA	5840	0.0	59.24658	4
TACGGGA	1980	0.0	58.585854	4
TAGGGAC	4115	0.0	58.323204	5
CGTAGGG	1955	0.0	58.312016	3
ACGGGAC	1290	0.0	57.984493	5
TAGCGGG	2915	0.0	57.907375	3
ATAGCGG	1000	0.0	57.6	2
GGGCGAT	3270	0.0	57.49235	7
ACGGGTA	565	0.0	57.34513	5
GCGGGAT	2680	0.0	57.16418	5
GCGGGTC	910	0.0	57.142857	5
GTAGGGA	5370	0.0	57.057728	4
TAGGGTC	2105	0.0	57.007122	5
>>END_MODULE