FastQCFastQC Report
Thu 26 May 2016
SRR1547264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547264_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1011001
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89360.8838764748996291No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC13920.13768532375338896No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11670.11543015288807824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG150.001309745180.02
TTGCGCG304.0596206E-880.01
TAATGCG550.080.01
GCGTAAG351.2896635E-980.01
TGCGCGG900.075.555552
CGTTTTT48400.075.4545441
GATTACG750.074.6666641
TAGGGCG1750.073.142865
GTTAGCG503.6379788E-1272.000011
GGCGATT2800.071.428578
CGTAAGG1700.070.5882342
GGGCGAT8350.069.940127
TAGCGGG4900.068.571433
GCGTCGT351.1813427E-768.5714380
TCGCGTG351.1813427E-768.571431
CGCGAGG1000.068.000012
GGACCGA1950.067.692318
GCGCGAG950.067.3684161
ACGGGAT2500.067.25
AGGGCGA5150.066.796116
Produced by FastQC (version 0.11.5)