FastQCFastQC Report
Thu 26 May 2016
SRR1547263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547263_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences890064
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80280.9019576120368873No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC150.001309693380.080
CGTTTTT44100.074.1950151
TAAACGG1100.069.090912
CGTAAGG1450.068.9655152
TAGGGAT14550.067.079045
TACGGGA3800.066.315794
ATAGGGA13800.064.3478244
GCGGGAT5700.063.859655
GGGCGAT5850.062.905987
CGAGGGA6700.062.686574
GTAGGGA13750.062.2545474
AGGGATT14650.062.252566
CTACGGG1300.061.5384643
AGGGACC9100.061.5384646
TAGGGAC11550.060.952385
TTAGGGA14250.060.912284
GCGCGAC200.0040981660.09
TAGCGAG800.060.01
ACGGGAC2800.060.05
CGCTTAA200.0040981660.080
Produced by FastQC (version 0.11.5)