##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547263_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	890064
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.012190134642005	33.0	31.0	34.0	31.0	34.0
2	32.4462566736774	34.0	31.0	34.0	31.0	34.0
3	32.54464398065757	34.0	31.0	34.0	31.0	34.0
4	36.097818808535116	37.0	35.0	37.0	35.0	37.0
5	36.0696399360046	37.0	35.0	37.0	35.0	37.0
6	36.121782253860395	37.0	36.0	37.0	35.0	37.0
7	36.325992288195	37.0	36.0	37.0	35.0	37.0
8	36.32016461737583	37.0	37.0	37.0	35.0	37.0
9	38.11190880655773	39.0	39.0	39.0	37.0	39.0
10-11	37.6796803375937	39.0	37.5	39.0	35.0	39.0
12-13	36.92383019648025	39.0	35.0	39.0	33.5	39.0
14-15	38.06345217871973	40.0	36.0	41.0	33.0	41.0
16-17	38.22390243847633	40.0	36.0	41.0	34.0	41.0
18-19	38.05685883262328	40.0	36.0	41.0	34.0	41.0
20-21	37.82912071491488	40.0	35.0	41.0	34.0	41.0
22-23	37.63916583526578	39.5	35.0	41.0	33.0	41.0
24-25	37.59775589171116	39.5	35.0	41.0	33.5	41.0
26-27	37.55318493950997	40.0	35.0	41.0	33.5	41.0
28-29	37.45680198277877	39.5	35.0	41.0	33.0	41.0
30-31	37.29182114993978	39.0	35.0	41.0	33.0	41.0
32-33	36.90883127505438	39.0	35.0	41.0	32.5	41.0
34-35	36.514854549785184	39.0	35.0	41.0	31.0	41.0
36-37	36.27437633698251	39.0	35.0	41.0	30.0	41.0
38-39	36.1512582241277	39.0	35.0	41.0	30.0	41.0
40-41	36.01420684355283	39.0	35.0	41.0	30.0	41.0
42-43	35.947053245609304	39.0	35.0	41.0	29.5	41.0
44-45	35.8001317882759	38.0	35.0	41.0	29.0	41.0
46-47	35.67271791691384	38.0	35.0	41.0	29.0	41.0
48-49	35.55483819141095	38.0	35.0	40.0	28.0	41.0
50-51	35.32451205755991	37.5	35.0	40.0	27.5	41.0
52-53	35.124955621168816	37.0	35.0	40.0	27.0	41.0
54-55	34.91772614104154	36.5	34.0	40.0	27.0	41.0
56-57	34.6309624925848	36.0	34.0	40.0	26.0	41.0
58-59	34.377916082439015	36.0	34.0	39.5	26.0	41.0
60-61	34.031397180427476	35.0	34.0	39.0	25.5	41.0
62-63	33.651525058872174	35.0	33.5	39.0	24.5	40.0
64-65	33.332617092703444	35.0	33.0	38.0	23.5	40.0
66-67	33.04050776123964	35.0	33.0	37.5	22.5	40.0
68-69	32.61761457603049	35.0	33.0	37.0	21.0	39.5
70-71	32.33519275018426	35.0	33.0	36.5	20.0	39.0
72-73	32.08989578277517	35.0	32.5	36.0	20.0	39.0
74-75	31.803469750489853	35.0	32.0	35.5	19.5	37.5
76-77	29.89955160527782	33.0	29.5	34.5	16.5	36.0
78-79	30.94865425407611	34.0	31.0	35.0	18.0	36.0
80-81	31.13013052993942	34.5	32.0	35.0	18.0	36.0
82-83	30.97740331032375	34.5	31.5	35.0	17.5	36.0
84-85	30.785778326052956	34.0	31.0	35.0	16.0	36.0
86	30.346270605259846	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	4.0
12	15.0
13	43.0
14	142.0
15	435.0
16	1111.0
17	2559.0
18	5758.0
19	8847.0
20	9984.0
21	9663.0
22	8337.0
23	7202.0
24	6913.0
25	7338.0
26	8242.0
27	9839.0
28	11768.0
29	14516.0
30	18381.0
31	22892.0
32	27647.0
33	37131.0
34	98140.0
35	90116.0
36	78094.0
37	134692.0
38	190179.0
39	79904.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.214272232109153	24.01860989771522	29.154869762174407	15.612248108001223
2	32.47923744809362	24.492620755361415	30.292540761113806	12.73560103543116
3	28.432562152833952	23.954569559042945	33.7964460982581	13.816422189865
4	25.990715274407233	27.103893652591278	32.91628467166406	13.98910640133743
5	23.976253393014435	31.517059447410524	31.241236585234322	13.26545057434072
6	22.5620854230707	39.88937874130399	27.360953819051215	10.187582016574089
7	87.29023980298047	3.2258354455409948	7.913138830466123	1.5707859210124213
8	87.75155494436355	2.7887882219705546	7.86943410810908	1.5902227255568138
9	83.15570565712129	5.613528914774668	8.853857700120441	2.3769077279836055
10-11	40.11132907296554	27.16074349709684	20.906361789713998	11.821565640223625
12-13	29.771735515648313	24.81956353700408	28.664230886767694	16.744470060579914
14-15	18.004716514767477	30.430620719408942	35.27083445684805	16.293828308975534
16-17	22.30682288015244	25.263969781948266	32.81775243128584	19.611454906613456
18-19	24.977754408671736	26.664936454007805	30.322650955436913	18.03465818188355
20-21	26.66184678854554	27.050470527962034	29.51641679699437	16.77126588649805
22-23	22.370807043089037	24.1057946394866	31.97230760934045	21.551090708083915
24-25	20.719015711229755	26.798410001977384	33.39596927861368	19.086605008179188
26-27	19.330576228226285	32.39677146811915	29.900209423142606	18.37244288051196
28-29	18.194421974150174	27.77755307483507	34.86429065775045	19.163734293264305
30-31	22.31401337431915	27.775811627029068	30.835703949378924	19.074471049272862
32-33	25.102576893346995	27.951304625285374	28.530195581441337	18.415922899926297
34-35	20.51599660249151	28.352567905229286	30.210524187024753	20.920911305254453
36-37	23.379217674234663	28.84736378507613	29.27154676517644	18.50187177551277
38-39	21.062137104747524	30.336807240827625	28.11365250139316	20.487403153031693
40-41	21.496150838591383	27.65160707544626	27.99360495425048	22.858637131711877
42-43	22.423162828740406	26.981037318664725	27.956978374588793	22.638821478006076
44-45	20.25534118894821	30.548702115802907	28.020625483111328	21.175331212137554
46-47	21.38323760987974	29.50186728145392	28.258979129590685	20.85591597907566
48-49	22.016506678171456	26.676227776878964	30.138843948300348	21.168421596649228
50-51	20.03063824623847	30.394106491218608	28.193197343112402	21.382057919430515
52-53	22.438217925901956	27.09018677308598	29.236549281849395	21.235046019162667
54-55	22.074648564597602	27.48914684786712	30.29557425084039	20.14063033669489
56-57	22.09459095076309	26.295805694871383	29.05465224972586	22.554951104639667
58-59	23.343714609286522	27.847491865753476	27.85299708784986	20.95579643711014
60-61	24.243200488953605	29.326374283197616	26.461467939384136	19.96895728846465
62-63	22.910712038684856	27.170967480990132	29.06892088658793	20.84939959373708
64-65	20.382860108935986	30.090869870031817	29.219808912617516	20.306461108414673
66-67	19.777903611425696	29.032406658397598	26.419392313361733	24.77029741681497
68-69	19.997831616602852	30.95766147153463	26.01161264807924	23.03289426378328
70-71	20.464989034496394	29.61573549767208	27.944226482590018	21.97504898524151
72-73	22.502651494723974	30.064860504413165	26.50303798378544	20.929450017077425
74-75	21.49244324003667	31.231405831490772	25.207288464649736	22.06886246382283
76-77	24.35010291394776	28.639738266012333	26.188622391198834	20.82153642884107
78-79	20.142877366121986	29.998011378957017	28.356331679519677	21.50277957540132
80-81	18.24138488917651	29.254300814323464	29.572704884143164	22.931609412356867
82-83	19.666450951841664	28.209207427780473	30.046603390318	22.07773823005986
84-85	18.37738634525158	27.946585863488465	28.722541300400874	24.953486490859085
86	19.71386327275342	27.746768771683833	29.723480558701397	22.81588739686135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	360.0
2	2268.0
3	2662.0
4	1355.5
5	1708.5
6	2160.0
7	2354.0
8	2477.5
9	2555.0
10	2452.0
11	2402.5
12	2450.5
13	2526.5
14	2562.0
15	2617.0
16	2718.0
17	2765.0
18	2790.5
19	2922.0
20	3192.5
21	3356.0
22	3447.5
23	3803.5
24	4549.0
25	5287.5
26	5750.5
27	6577.5
28	8533.5
29	9868.0
30	10791.5
31	12648.0
32	14535.0
33	15539.0
34	17408.5
35	20407.5
36	21587.0
37	23033.5
38	24613.0
39	26819.5
40	30193.0
41	34539.0
42	39624.0
43	41663.0
44	43022.0
45	44727.0
46	46046.5
47	47651.5
48	46983.0
49	42604.0
50	39525.0
51	37394.0
52	32838.5
53	29352.5
54	27511.5
55	25394.0
56	23602.0
57	23148.0
58	20805.5
59	16705.0
60	12980.5
61	9798.5
62	7483.5
63	5799.0
64	5214.0
65	4547.5
66	3389.0
67	2590.0
68	2009.5
69	1700.0
70	1492.5
71	1321.0
72	1019.0
73	637.0
74	492.0
75	322.5
76	192.5
77	148.5
78	90.0
79	50.0
80	43.5
81	27.0
82	15.0
83	13.0
84	9.0
85	4.0
86	3.0
87	4.0
88	5.5
89	4.0
90	1.0
91	0.0
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	890064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.439747176177292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07213760110152	18.299911668735444
2	6.243642492471553	2.9269880296394124
3	2.3785845041157363	1.6726025824093766
4	1.3605272708010216	1.2756166101548179
5	0.9460401401539899	1.1087470851862429
6	0.7222994416132107	1.0158309778144616
7	0.5584943779450952	0.9163676912844605
8	0.5015103042921663	0.9404219791084896
9	0.43608697231755505	0.9199591540153305
>10	6.970655722012256	40.47045938949024
>50	1.7167161287256099	26.800953559983675
>100	0.09282159862416293	2.7424205196556084
>500	0.0	0.0
>1k	0.0	0.0
>5k	4.8344582616751525E-4	0.9097207525224256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8028	0.9019576120368873	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1235147135486887E-4	0.0	0.0	0.03437955023458987	0.0
2	1.1235147135486887E-4	0.0	0.0	0.08740944471408797	0.0
3	1.1235147135486887E-4	0.0	0.0	0.14223696273526398	0.0
4	1.1235147135486887E-4	0.0	0.0	0.22728702655089972	0.0
5	1.1235147135486887E-4	0.0	0.0	0.42300328965108125	0.0
6	1.1235147135486887E-4	0.0	0.0	0.6351228675690737	0.0
7	1.1235147135486887E-4	0.0	0.0	0.781404483273113	0.0
8	1.1235147135486887E-4	0.0	0.0	1.0390264070898272	0.0
9	1.1235147135486887E-4	0.0	0.0	1.1428391666217261	0.0
10	1.1235147135486887E-4	0.0	0.0	1.3135010516097718	0.0
11	1.1235147135486887E-4	0.0	0.0	1.5653930503873879	0.0
12	1.1235147135486887E-4	0.0	0.0	1.7636933973287314	0.0
13	1.1235147135486887E-4	0.0	0.0	1.8468334861313345	0.0
14	1.1235147135486887E-4	0.0	0.0	1.8830106599076022	0.0
15	1.1235147135486887E-4	0.0	0.0	1.931209441118841	0.0
16	1.1235147135486887E-4	0.0	0.0	2.030528141796545	0.0
17	1.1235147135486887E-4	0.0	0.0	2.1560247352999333	0.0
18	1.1235147135486887E-4	0.0	0.0	2.3076992216290066	0.0
19	1.1235147135486887E-4	0.0	0.0	2.4110625752754857	0.0
20	1.1235147135486887E-4	0.0	0.0	2.5116171421380935	0.0
21	1.1235147135486887E-4	0.0	0.0	2.6449783386363226	0.0
22	1.1235147135486887E-4	0.0	0.0	2.785305326358554	0.0
23	1.1235147135486887E-4	0.0	0.0	2.9423726833126604	0.0
24	1.1235147135486887E-4	0.0	0.0	3.0677569253446944	0.0
25	1.1235147135486887E-4	0.0	0.0	3.1760637437307877	0.0
26	1.1235147135486887E-4	0.0	0.0	3.27718006795017	0.0
27	1.1235147135486887E-4	0.0	0.0	3.3912168113753616	0.0
28	1.1235147135486887E-4	0.0	0.0	3.5112081827823616	0.0
29	1.1235147135486887E-4	0.0	0.0	3.647827571949882	0.0
30	1.1235147135486887E-4	0.0	0.0	3.790401589099211	0.0
31	1.1235147135486887E-4	0.0	0.0	3.9370202592173147	0.0
32	1.1235147135486887E-4	0.0	0.0	4.058022793866509	0.0
33	1.1235147135486887E-4	0.0	0.0	4.1856540653256396	0.0
34	1.1235147135486887E-4	0.0	0.0	4.3249698898056765	0.0
35	1.1235147135486887E-4	0.0	0.0	4.484846033543655	0.0
36	1.1235147135486887E-4	0.0	0.0	4.63494759927376	0.0
37	1.1235147135486887E-4	0.0	0.0	4.792352010641931	0.0
38	1.1235147135486887E-4	0.0	0.0	4.967058548598753	0.0
39	1.1235147135486887E-4	0.0	0.0	5.220523467975337	0.0
40	1.1235147135486887E-4	0.0	0.0	5.41466681047655	0.0
41	1.1235147135486887E-4	0.0	0.0	5.6069001779647305	0.0
42	1.1235147135486887E-4	0.0	0.0	5.790707185101296	0.0
43	1.1235147135486887E-4	0.0	0.0	5.954290927393985	0.0
44	1.1235147135486887E-4	0.0	0.0	6.131019791835194	0.0
45	1.1235147135486887E-4	0.0	0.0	6.299996404752917	0.0
46	1.1235147135486887E-4	0.0	0.0	6.489870391342645	0.0
47	1.1235147135486887E-4	0.0	0.0	6.684013733843859	0.0
48	1.1235147135486887E-4	0.0	0.0	6.862540221826745	0.0
49	1.1235147135486887E-4	0.0	0.0	7.041853170109116	0.0
50	1.1235147135486887E-4	0.0	0.0	7.232401265526973	0.0
51	1.1235147135486887E-4	0.0	0.0	7.431937478653221	0.0
52	1.1235147135486887E-4	0.0	0.0	7.665179133185928	0.0
53	1.1235147135486887E-4	0.0	0.0	7.861794208056948	0.0
54	1.1235147135486887E-4	0.0	0.0	8.065487425623326	0.0
55	1.1235147135486887E-4	0.0	0.0	8.273112944687124	0.0
56	1.1235147135486887E-4	0.0	0.0	8.474334429883694	0.0
57	1.1235147135486887E-4	0.0	0.0	8.674881806252134	0.0
58	1.1235147135486887E-4	0.0	0.0	8.87026101493825	0.0
59	1.1235147135486887E-4	0.0	0.0	9.079459454601018	0.0
60	1.1235147135486887E-4	0.0	0.0	9.293039601646623	0.0
61	1.1235147135486887E-4	0.0	0.0	9.510439698718294	0.0
62	1.1235147135486887E-4	0.0	0.0	9.75794999011307	0.0
63	1.1235147135486887E-4	0.0	0.0	10.009055528591203	0.0
64	1.1235147135486887E-4	0.0	0.0	10.246791241978105	0.0
65	1.1235147135486887E-4	0.0	0.0	10.475988243542037	0.0
66	1.1235147135486887E-4	0.0	0.0	10.69754534505384	0.0
67	1.1235147135486887E-4	0.0	0.0	10.920338312750543	0.0
68	1.1235147135486887E-4	0.0	0.0	11.172342662999515	0.0
69	1.1235147135486887E-4	0.0	0.0	11.497712524043214	0.0
70	1.1235147135486887E-4	0.0	0.0	11.743649894839022	0.0
71	1.1235147135486887E-4	0.0	0.0	11.971723381689406	0.0
72	1.1235147135486887E-4	0.0	0.0	12.196988081755919	0.0
73	1.1235147135486887E-4	0.0	0.0	12.440116665767855	0.0
74	1.1235147135486887E-4	0.0	0.0	12.682121735066243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGTC	15	0.0013096933	80.0	80
CGTTTTT	4410	0.0	74.195015	1
TAAACGG	110	0.0	69.09091	2
CGTAAGG	145	0.0	68.965515	2
TAGGGAT	1455	0.0	67.07904	5
TACGGGA	380	0.0	66.31579	4
ATAGGGA	1380	0.0	64.347824	4
GCGGGAT	570	0.0	63.85965	5
GGGCGAT	585	0.0	62.90598	7
CGAGGGA	670	0.0	62.68657	4
GTAGGGA	1375	0.0	62.254547	4
AGGGATT	1465	0.0	62.25256	6
CTACGGG	130	0.0	61.538464	3
AGGGACC	910	0.0	61.538464	6
TAGGGAC	1155	0.0	60.95238	5
TTAGGGA	1425	0.0	60.91228	4
GCGCGAC	20	0.00409816	60.0	9
TAGCGAG	80	0.0	60.0	1
ACGGGAC	280	0.0	60.0	5
CGCTTAA	20	0.00409816	60.0	80
>>END_MODULE