FastQCFastQC Report
Thu 26 May 2016
SRR1547262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547262_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2523068
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376651.492825401455688No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC38570.15286944307485964No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25520.10114669917735074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT147600.075.691061
GGACGTA600.073.333338
AGGGCGA9350.071.871666
GGTTACG800.070.01
CGAACGG1500.069.3333362
CGAGGGA15650.067.987224
ACGGGAT14100.067.517735
GCGAACG1250.067.21
CGGGATT15300.067.1895456
GCGGGAT17450.067.163325
TACGGGA9950.067.135684
ACGAGGG14800.066.756763
CTTCGCG303.7968985E-666.6666641
TACGCGG1200.066.6666642
TTACGGG7400.066.486493
GTCGACG559.094947E-1265.4545442
ACGGGAC9000.065.3333365
GCGATAT1600.065.09
TAGTGCG1550.064.516131
TAGGGAT48400.064.132235
Produced by FastQC (version 0.11.5)