##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547262_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2523068
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10729278798669	33.0	31.0	34.0	31.0	34.0
2	32.520961781450204	34.0	31.0	34.0	31.0	34.0
3	32.584096029120104	34.0	31.0	34.0	31.0	34.0
4	36.123786199975584	37.0	35.0	37.0	35.0	37.0
5	36.09346795250861	37.0	35.0	37.0	35.0	37.0
6	36.158691323420534	37.0	36.0	37.0	35.0	37.0
7	36.4128374661325	37.0	37.0	37.0	35.0	37.0
8	36.42178292459815	37.0	37.0	37.0	35.0	37.0
9	38.23698608202395	39.0	39.0	39.0	37.0	39.0
10-11	37.75019856777542	39.0	37.5	39.0	35.0	39.0
12-13	37.03983325062978	39.0	35.0	39.0	33.5	39.0
14-15	38.20920541975087	40.0	36.0	41.0	33.5	41.0
16-17	38.38818731797954	40.0	36.0	41.0	34.0	41.0
18-19	38.2197485363058	40.0	36.0	41.0	34.0	41.0
20-21	37.9938285452473	40.0	35.0	41.0	34.0	41.0
22-23	37.799127688988165	40.0	35.0	41.0	34.0	41.0
24-25	37.75570119394325	40.0	35.0	41.0	34.0	41.0
26-27	37.7205142707212	40.0	35.0	41.0	34.0	41.0
28-29	37.63632470468493	40.0	35.0	41.0	34.0	41.0
30-31	37.431930887316554	39.5	35.0	41.0	33.5	41.0
32-33	37.031415919031915	39.0	35.0	41.0	33.0	41.0
34-35	36.65558756244382	39.0	35.0	41.0	32.0	41.0
36-37	36.42892581571326	39.0	35.0	41.0	31.0	41.0
38-39	36.33259527686135	39.0	35.0	41.0	31.0	41.0
40-41	36.184561811255186	39.0	35.0	41.0	30.5	41.0
42-43	36.153104276222436	39.0	35.0	41.0	31.0	41.0
44-45	36.027269974491375	38.5	35.0	41.0	30.0	41.0
46-47	35.88363789640232	38.0	35.0	41.0	30.0	41.0
48-49	35.79950738545295	38.0	35.0	40.0	30.0	41.0
50-51	35.57036056895811	38.0	35.0	40.0	29.0	41.0
52-53	35.38717050035909	37.5	35.0	40.0	28.5	41.0
54-55	35.23053203480842	37.0	35.0	40.0	28.0	41.0
56-57	34.96332976360526	36.5	34.5	40.0	27.5	41.0
58-59	34.71072400743856	36.0	34.0	40.0	26.5	41.0
60-61	34.34031722490238	36.0	34.0	39.5	26.0	41.0
62-63	33.90620268657048	35.0	33.5	39.0	25.0	40.5
64-65	33.4483248172463	35.0	33.0	38.5	22.5	40.0
66-67	33.047608903129046	35.0	33.0	38.0	21.0	40.0
68-69	32.4770204370235	35.0	33.0	37.0	18.5	39.5
70-71	32.03169514258038	35.0	33.0	36.5	16.5	39.0
72-73	31.675655987076052	35.0	32.0	36.0	13.0	39.0
74-75	31.328370856433516	35.0	32.0	36.0	11.5	38.0
76-77	29.407205037676352	33.0	29.0	34.5	10.0	36.0
78-79	30.293542227161538	34.0	30.5	35.0	10.0	36.5
80-81	30.3574602428472	35.0	31.0	35.0	10.0	36.0
82-83	30.09491737836634	34.5	31.0	35.0	10.0	36.0
84-85	29.81998166517906	34.0	30.5	35.0	10.0	36.0
86	29.424913240546825	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	16.0
12	20.0
13	70.0
14	246.0
15	835.0
16	2669.0
17	6692.0
18	16514.0
19	25723.0
20	27225.0
21	24183.0
22	20132.0
23	16558.0
24	16692.0
25	18469.0
26	22461.0
27	27687.0
28	35200.0
29	45217.0
30	59347.0
31	74870.0
32	92311.0
33	114927.0
34	272458.0
35	274459.0
36	214312.0
37	344963.0
38	516337.0
39	251937.0
40	528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.457351129656434	23.283835394052	31.457812472751428	15.801001003540135
2	31.261820925952055	24.886051426279433	31.32079674428117	12.531330903487342
3	27.3861029508519	24.90681979241146	35.68663230638255	12.020444950354092
4	25.32345541222036	26.21498905301007	34.723836218445165	13.737719316324412
5	23.211582089741537	31.155402866668673	32.51026131677783	13.122753726811961
6	21.463749688870852	39.9324552489271	28.056437638620917	10.547357423581131
7	87.1951925195833	2.786567781764106	8.586134024132525	1.4321056745200684
8	87.86572537878487	2.0921750820826075	8.35260880800676	1.6894907311257565
9	84.18627639048968	4.516604387991128	9.35091721665845	1.946202004860749
10-11	39.45224623355375	27.386142585138412	22.158776537136536	11.002834644171303
12-13	27.284678811668968	26.124523794047565	30.451161046789068	16.1396363474944
14-15	14.711712090201296	32.38737521144892	36.76284190517259	16.138070793177196
16-17	19.714391367969473	27.041661183923697	34.467560921861796	18.77638652624503
18-19	21.39296285316131	28.660919959351077	32.163877469810565	17.78223971767705
20-21	23.891944251997966	29.84005980021149	30.754442607175076	15.513553340615474
22-23	19.52501874701752	26.586481220482366	32.7503658244645	21.138134208035613
24-25	17.56016881035311	29.184924861319633	35.60542165332048	17.649484675006775
26-27	16.659955260817387	35.175231107524645	30.836901740262253	17.327911891395715
28-29	15.388388263812153	30.05816727888428	36.25853524360026	18.294909213703317
30-31	20.5436199103631	30.519767996740477	31.584761092447767	17.351851000448658
32-33	23.506223375668036	30.58591762092817	29.082549499260423	16.82530950414337
34-35	18.420648987661053	31.15304462662124	30.608489347096473	19.817817038621236
36-37	21.098678275813416	31.39037473425211	30.333863375858282	17.177083614076196
38-39	18.873589613914486	33.34089687634261	28.455455818075453	19.330057691667445
40-41	19.966762687331453	29.563095406069117	28.982294571529582	21.487847335069844
42-43	19.442123636778717	29.224499696401363	29.11465327133474	22.218723395485178
44-45	18.60056883128001	32.80494223699083	28.015634140657326	20.57885479107182
46-47	19.865992513875963	31.857762057938988	28.748135206819637	19.528110221365417
48-49	20.04004648309122	28.644511364735315	31.153460786629612	20.161981365543856
50-51	18.429091090688004	31.921216550643898	28.88697411246942	20.762718246198673
52-53	20.766701491993082	28.839531871515156	30.57468130070216	19.8190853357896
54-55	20.57643709959462	28.622454884291663	31.75473273015234	19.04637528596138
56-57	20.942638089817635	27.599018337991684	30.23481729386604	21.22352627832464
58-59	22.102297678857642	29.654274082188824	29.161263192272262	19.08216504668126
60-61	21.90254087483968	31.254429131517657	27.445019317751246	19.398010675891413
62-63	21.088412995606937	29.6154126642643	30.803410768160035	18.49276357196873
64-65	18.111739358590416	33.59881699581621	29.689588231470577	18.599855414122807
66-67	17.48797495747241	32.56099320351255	26.59561692352327	23.355414915491775
68-69	17.196722403042646	34.34960928520357	26.021038671966036	22.432629639787752
70-71	17.801224540916056	33.22585835974298	28.68646425700774	20.286452842333222
72-73	20.24029871569058	33.02094909847852	27.16351679780331	19.575235388027593
74-75	19.466974334421426	34.749301247528805	25.52360855910344	20.26011585894633
76-77	22.75820548633648	31.834675086046037	26.509987047515164	18.89713238010232
78-79	18.14897577076797	32.26106074033677	29.333196727159155	20.25676676173611
80-81	16.253901995507057	31.847040983437623	30.252078025641797	21.64697899541352
82-83	17.766187831639893	30.898671775790426	30.502903607829836	20.832236784739848
84-85	16.202000897320247	31.010975526620765	29.360068773413957	23.426954802645035
86	17.45268062533392	30.86615184370774	30.19843301884848	21.48273451210986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1133.0
1	1794.0
2	13162.5
3	13896.0
4	4528.0
5	7078.5
6	11280.5
7	13538.0
8	15536.0
9	17693.0
10	16989.5
11	14831.0
12	12741.5
13	11428.5
14	10909.0
15	10767.5
16	10559.5
17	10358.0
18	10414.0
19	10698.5
20	10991.0
21	11190.0
22	11399.5
23	12624.0
24	14024.0
25	15500.0
26	18355.0
27	21385.0
28	26636.0
29	30621.0
30	32132.0
31	36051.0
32	42574.5
33	50014.0
34	56226.5
35	63250.0
36	67385.0
37	73294.0
38	88916.0
39	100266.5
40	109743.5
41	123810.0
42	135146.5
43	140256.0
44	140460.0
45	136655.5
46	135596.5
47	140535.5
48	135108.0
49	114126.0
50	100561.0
51	88149.0
52	69606.0
53	59217.0
54	51978.5
55	48003.0
56	43652.0
57	40296.0
58	36465.5
59	27357.5
60	18121.5
61	12753.5
62	9946.5
63	6835.0
64	5121.0
65	4281.5
66	2754.5
67	1479.5
68	787.5
69	628.5
70	520.0
71	466.0
72	432.0
73	297.5
74	181.5
75	156.5
76	123.5
77	81.5
78	36.5
79	10.0
80	31.0
81	29.0
82	6.5
83	6.0
84	2.5
85	0.0
86	0.0
87	0.0
88	0.5
89	1.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2523068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.851290080533467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81719159415492	15.050690634269529
2	9.640876199961953	3.827676601519118
3	3.626129254809792	2.159500311202135
4	1.8826776538696794	1.4949432094042074
5	1.185197761673828	1.1763852284893064
6	0.8661027243009193	1.0315953851782709
7	0.6555653286798714	0.9109672254455075
8	0.5425153312395942	0.8615703370859108
9	0.41509872358676664	0.7416220656582077
>10	3.1171242486189943	13.303641612186407
>50	0.9576004030775388	13.899874748984736
>100	1.2876611731782122	42.68120033951225
>500	0.003432685432753114	0.4737958995288816
>1k	0.002624994742693558	0.8767629196611868
>5k	0.0	0.0
>10k+	2.0192267251488904E-4	1.509773481874393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37665	1.492825401455688	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC	3857	0.15286944307485964	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2552	0.10114669917735074	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9634286511501075E-5	0.0	0.0	0.09908571627875269	0.0
2	3.9634286511501075E-5	0.0	0.0	0.275180851249352	0.0
3	3.9634286511501075E-5	0.0	0.0	0.4084709567875301	0.0
4	3.9634286511501075E-5	0.0	0.0	0.6400144585877194	0.0
5	3.9634286511501075E-5	0.0	0.0	1.2286628818565335	0.0
6	3.9634286511501075E-5	0.0	0.0	1.7009054056410686	0.0
7	3.9634286511501075E-5	0.0	0.0	2.0660560872715283	0.0
8	3.9634286511501075E-5	0.0	0.0	2.7569213354535034	0.0
9	3.9634286511501075E-5	0.0	0.0	3.032696701000528	0.0
10	3.9634286511501075E-5	0.0	0.0	3.474698264176788	0.0
11	3.9634286511501075E-5	0.0	0.0	4.301390212233677	0.0
12	3.9634286511501075E-5	0.0	0.0	4.870498932252321	0.0
13	3.9634286511501075E-5	0.0	0.0	5.0691063419614535	0.0
14	3.9634286511501075E-5	0.0	0.0	5.145481612069116	0.0
15	3.9634286511501075E-5	0.0	0.0	5.255228951419462	0.0
16	3.9634286511501075E-5	0.0	0.0	5.4924401561907965	0.0
17	3.9634286511501075E-5	0.0	0.0	5.827389511499492	0.0
18	3.9634286511501075E-5	0.0	0.0	6.198762776112257	0.0
19	3.9634286511501075E-5	0.0	0.0	6.471525935884407	0.0
20	3.9634286511501075E-5	0.0	0.0	6.734103084023102	0.0
21	3.9634286511501075E-5	0.0	0.0	7.040832827335609	0.0
22	3.9634286511501075E-5	0.0	0.0	7.385096239974507	0.0
23	3.9634286511501075E-5	0.0	0.0	7.741408475712902	0.0
24	3.9634286511501075E-5	0.0	0.0	7.997763040869291	0.0
25	3.9634286511501075E-5	0.0	0.0	8.21238270232907	0.0
26	3.9634286511501075E-5	0.0	0.0	8.411584626335873	0.0
27	3.9634286511501075E-5	0.0	0.0	8.626521362087743	0.0
28	3.9634286511501075E-5	0.0	0.0	8.851445938040513	0.0
29	3.9634286511501075E-5	0.0	0.0	9.116321874796874	0.0
30	3.9634286511501075E-5	0.0	0.0	9.43093884112517	0.0
31	3.9634286511501075E-5	0.0	0.0	9.71083617246939	0.0
32	3.9634286511501075E-5	0.0	0.0	9.94638273720724	0.0
33	3.9634286511501075E-5	0.0	0.0	10.182880524821368	0.0
34	3.9634286511501075E-5	0.0	0.0	10.446210724403782	0.0
35	3.9634286511501075E-5	0.0	0.0	10.743071530374925	0.0
36	3.9634286511501075E-5	0.0	0.0	11.015240175849403	0.0
37	3.9634286511501075E-5	0.0	0.0	11.302073507333136	0.0
38	7.926857302300215E-5	0.0	0.0	11.590888553142443	0.0
39	7.926857302300215E-5	0.0	0.0	11.975182595157959	0.0
40	7.926857302300215E-5	0.0	0.0	12.29312884155322	0.0
41	7.926857302300215E-5	0.0	0.0	12.597163453382946	0.0
42	7.926857302300215E-5	0.0	0.0	12.9109480996945	0.0
43	7.926857302300215E-5	0.0	0.0	13.200476562661015	0.0
44	7.926857302300215E-5	0.0	0.0	13.48972758562195	0.0
45	7.926857302300215E-5	0.0	0.0	13.789600597367967	0.0
46	7.926857302300215E-5	0.0	0.0	14.11127246669531	0.0
47	7.926857302300215E-5	0.0	0.0	14.432270553151957	0.0
48	7.926857302300215E-5	0.0	0.0	14.742844822256078	0.0
49	7.926857302300215E-5	0.0	0.0	15.064595960156444	0.0
50	7.926857302300215E-5	0.0	0.0	15.391856263881909	0.0
51	7.926857302300215E-5	0.0	0.0	15.716897047562728	0.0
52	7.926857302300215E-5	0.0	0.0	16.080977603457377	0.0
53	7.926857302300215E-5	0.0	0.0	16.404155575672156	0.0
54	7.926857302300215E-5	0.0	0.0	16.74528787967665	0.0
55	7.926857302300215E-5	0.0	0.0	17.087093966551834	0.0
56	7.926857302300215E-5	0.0	0.0	17.421607344708903	0.0
57	7.926857302300215E-5	0.0	0.0	17.74981887131064	0.0
58	7.926857302300215E-5	0.0	0.0	18.06027423755523	0.0
59	7.926857302300215E-5	0.0	0.0	18.400494952969957	0.0
60	7.926857302300215E-5	0.0	0.0	18.759621223050665	0.0
61	7.926857302300215E-5	0.0	0.0	19.133967059151793	0.0
62	7.926857302300215E-5	0.0	0.0	19.54390448454025	0.0
63	7.926857302300215E-5	0.0	0.0	19.964820607292392	0.0
64	7.926857302300215E-5	0.0	0.0	20.344120729207457	0.0
65	7.926857302300215E-5	0.0	0.0	20.707844576523502	0.0
66	7.926857302300215E-5	0.0	0.0	21.04834273194381	0.0
67	7.926857302300215E-5	0.0	0.0	21.391337847414338	0.0
68	7.926857302300215E-5	0.0	0.0	21.768259912138713	0.0
69	7.926857302300215E-5	0.0	0.0	22.231188378593046	0.0
70	7.926857302300215E-5	0.0	0.0	22.58857074006725	0.0
71	1.1890285953450323E-4	0.0	0.0	22.947498838715404	0.0
72	1.585371460460043E-4	0.0	0.0	23.305990960211933	0.0
73	1.585371460460043E-4	0.0	0.0	23.67764166483028	0.0
74	1.585371460460043E-4	0.0	0.0	24.074896118535055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14760	0.0	75.69106	1
GGACGTA	60	0.0	73.33333	8
AGGGCGA	935	0.0	71.87166	6
GGTTACG	80	0.0	70.0	1
CGAACGG	150	0.0	69.333336	2
CGAGGGA	1565	0.0	67.98722	4
ACGGGAT	1410	0.0	67.51773	5
GCGAACG	125	0.0	67.2	1
CGGGATT	1530	0.0	67.189545	6
GCGGGAT	1745	0.0	67.16332	5
TACGGGA	995	0.0	67.13568	4
ACGAGGG	1480	0.0	66.75676	3
CTTCGCG	30	3.7968985E-6	66.666664	1
TACGCGG	120	0.0	66.666664	2
TTACGGG	740	0.0	66.48649	3
GTCGACG	55	9.094947E-12	65.454544	2
ACGGGAC	900	0.0	65.333336	5
GCGATAT	160	0.0	65.0	9
TAGTGCG	155	0.0	64.51613	1
TAGGGAT	4840	0.0	64.13223	5
>>END_MODULE