FastQCFastQC Report
Thu 26 May 2016
SRR1547261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547261_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1486047
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183181.2326662615650785No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC22540.15167757143616586No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC22130.1489185739078239No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG21660.14575582064362702No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20510.13801716903974098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG304.0610757E-880.000011
GCGATAT700.080.09
TAATGCG251.2849905E-680.01
CGTTTTT75050.074.457021
ACGATTG850.070.588241
TATTACG1200.070.000011
GTCGACG800.070.01
TATTGCG1500.069.3333361
GTAGGGT6350.068.6614154
GGCGATA3000.068.08
AGGGCGA6000.068.06
TTACGGG5150.067.5728153
TAGGGTA6650.067.368425
TAGGGCG2750.066.909095
GTTACGG900.066.666672
ACGGGAT4950.066.262625
TACGGGT1150.066.086964
GCGCGAC559.094947E-1265.4545449
TAACGGG4100.065.365853
CGAGGGA7900.064.810134
Produced by FastQC (version 0.11.5)