##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547261_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1486047
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98617674945678	33.0	31.0	34.0	30.0	34.0
2	32.393974753153834	33.0	31.0	34.0	31.0	34.0
3	32.48038319111038	34.0	31.0	34.0	31.0	34.0
4	36.036281490423924	37.0	35.0	37.0	35.0	37.0
5	36.008581828165596	37.0	35.0	37.0	35.0	37.0
6	36.057810419185934	37.0	35.0	37.0	35.0	37.0
7	36.30937850552506	37.0	36.0	37.0	35.0	37.0
8	36.30338542455252	37.0	37.0	37.0	35.0	37.0
9	38.071232605698206	39.0	39.0	39.0	37.0	39.0
10-11	37.60728193657401	39.0	37.5	39.0	35.0	39.0
12-13	36.59072559616217	39.0	35.0	39.0	33.0	39.0
14-15	37.64406442057351	40.0	35.0	41.0	33.0	41.0
16-17	37.866502876423155	40.0	35.0	41.0	34.0	41.0
18-19	37.73365041617123	39.0	36.0	41.0	34.0	41.0
20-21	37.48026071853717	39.0	35.0	41.0	34.0	41.0
22-23	37.25573181736513	39.0	35.0	41.0	33.0	41.0
24-25	37.189966064330406	38.5	35.0	41.0	33.0	41.0
26-27	37.089205455816675	38.0	35.0	41.0	33.0	41.0
28-29	37.013205167804244	38.0	35.0	41.0	33.0	41.0
30-31	36.845503204138225	38.0	35.0	41.0	33.0	41.0
32-33	36.45047330266136	38.0	35.0	41.0	31.5	41.0
34-35	36.064000331079704	38.0	35.0	41.0	31.0	41.0
36-37	35.81351733828069	38.0	35.0	41.0	30.0	41.0
38-39	35.75897734055518	37.5	35.0	40.0	30.0	41.0
40-41	35.59796964698963	37.0	35.0	40.0	29.5	41.0
42-43	35.59447278585401	37.0	35.0	40.0	30.0	41.0
44-45	35.48104265881227	37.0	35.0	40.0	29.5	41.0
46-47	35.31767837760178	37.0	35.0	40.0	29.0	41.0
48-49	35.236883490226084	36.0	35.0	40.0	29.0	41.0
50-51	35.00508698580866	36.0	35.0	40.0	27.5	41.0
52-53	34.805485627305195	36.0	34.0	40.0	27.0	41.0
54-55	34.63646741994029	35.5	34.0	40.0	27.0	41.0
56-57	34.36102592986629	35.0	34.0	39.5	26.0	41.0
58-59	34.05690566987451	35.0	34.0	39.0	25.5	41.0
60-61	33.604307602653215	35.0	33.5	38.5	24.5	40.0
62-63	33.030048511251664	35.0	32.5	38.0	22.5	40.0
64-65	32.51926284969453	35.0	32.5	37.0	20.0	40.0
66-67	32.08351788335093	35.0	32.0	36.5	18.5	39.5
68-69	31.61512152711186	35.0	32.0	36.0	14.0	39.0
70-71	31.21590097755993	35.0	31.0	35.5	11.5	38.5
72-73	30.900203694768738	35.0	31.0	35.0	10.5	38.0
74-75	30.607405754999675	35.0	31.0	35.0	10.0	37.0
76-77	28.759162395267445	32.5	27.5	34.5	10.0	35.5
78-79	29.60595257081371	34.0	29.0	35.0	10.0	36.0
80-81	29.863586077694716	34.0	30.0	35.0	10.0	36.0
82-83	29.671726398963155	34.0	29.5	35.0	10.0	36.0
84-85	29.45642297989229	34.0	29.0	35.0	8.0	35.0
86	29.068083983884762	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	2.0
10	1.0
11	5.0
12	20.0
13	60.0
14	205.0
15	669.0
16	1856.0
17	4242.0
18	8968.0
19	13741.0
20	15230.0
21	14912.0
22	13113.0
23	12266.0
24	12984.0
25	14821.0
26	17905.0
27	21829.0
28	26946.0
29	34437.0
30	43674.0
31	52902.0
32	60138.0
33	71593.0
34	202028.0
35	194023.0
36	111987.0
37	183604.0
38	247287.0
39	104364.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.778093828795452	25.093957324364574	29.783983952055355	16.34396489478462
2	29.956925992246543	26.452729960761673	30.78987407531525	12.800469971676534
3	27.94319425967012	24.49545673858229	35.040345291905304	12.521003709842285
4	25.555988471427888	26.842017782748464	34.05296063987209	13.54903310595156
5	23.912231578139856	31.645096016478618	30.827557943995043	13.615114461386485
6	23.032380537089338	37.874374094493646	28.021792042916545	11.071453325500471
7	85.09771225270802	4.026857831549069	8.86950412739301	2.0059257883498978
8	85.2926589805033	3.494505893824354	8.661771801295652	2.551063324376685
9	80.72671994896527	6.067170150069278	10.243888652243166	2.9622212487222814
10-11	45.61141067543624	24.09193652690662	19.17654690598615	11.12010589167099
12-13	29.768304770979654	30.242314004873332	25.27739701368799	14.711984210459022
14-15	14.130609597139257	36.05929018395784	35.9055938338424	13.9045063850605
16-17	16.155410966140373	26.176594683748228	35.40685456112761	22.261139788983794
18-19	22.00236600861211	29.208833906330018	30.208162998882273	18.580637086175606
20-21	24.54626266867737	30.13292984676797	27.96358392433079	17.35722356022387
22-23	19.370988939111616	29.20324861865069	27.96529988620817	23.460462556029523
24-25	17.372263461384467	28.74246238510626	35.33007367869253	18.555200474816747
26-27	16.56825120605203	37.850014165097065	27.526383755022554	18.055350873828353
28-29	14.653910677118557	29.93270064809525	36.321125778659756	19.092262896126435
30-31	21.604733901417653	32.63530695866281	27.723584785676366	18.036374354243172
32-33	26.160814563738562	31.315429458153073	25.297921263593953	17.225834714514413
34-35	19.590598413105372	30.60323798641631	28.106446162200793	21.699717438277524
36-37	23.250913329120813	31.267079708784447	28.96496544187364	16.517041520221095
38-39	20.481418151646615	32.90834677503471	27.59633443625942	19.01390063705926
40-41	20.112351762763897	29.573122519005118	27.92532806835854	22.389197649872447
42-43	21.235499280978328	28.09376150283268	27.236352551433434	23.434386664755554
44-45	18.044886871007446	36.24124943558313	25.363464278047733	20.350399415361693
46-47	21.253870166959725	32.47582344300012	26.535600825545895	19.73470556449426
48-49	21.121270054042704	27.336753144416026	30.918066521449187	20.623910280092083
50-51	18.438649652399956	33.51603280380769	26.462218220554263	21.583099323238095
52-53	21.65008912907869	27.99837421023696	30.208062059948304	20.14347460073605
54-55	21.950146933441538	29.032022540336882	30.332788936016154	18.685041590205422
56-57	22.44966007131672	26.64498498365126	28.746735466644058	22.158619478387966
58-59	23.65042962974926	29.94366934558597	27.276526247151	19.129374777513767
60-61	23.736799710910894	33.103428088075276	25.015258602184183	18.144513598829647
62-63	22.44636273280724	29.606129550411257	29.6380262535438	18.309481463237702
64-65	17.904379874929933	35.61243352330041	28.77408991774823	17.709096684021432
66-67	16.69519873866708	32.82880689507129	24.084332460548016	26.391661905713615
68-69	16.63241472174164	36.91040727514002	22.77189079484027	23.68528720827807
70-71	18.302684908350813	33.687797223102635	27.05412412931758	20.955393739228974
72-73	21.6730695597111	34.09602791836328	24.69995901879281	19.530943503132807
74-75	20.90495791855843	35.85603281726621	22.412346312061462	20.826662952113896
76-77	26.57664932535781	31.057597774498387	23.82794756828014	18.537805331863662
78-79	18.331688028709724	32.628274879596674	28.679846599737424	20.360190491956175
80-81	15.116379226229048	31.52036914041077	30.524606556858565	22.838645076501617
82-83	18.23384455538755	29.07996853396965	31.045417809800096	21.640769100842704
84-85	16.030112102780063	29.637185095760763	28.279724665505196	26.052978135953975
86	17.33841527219529	28.95581364519426	30.8145031752024	22.891267907408043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	727.0
1	1088.0
2	6906.0
3	7454.5
4	3207.5
5	5625.0
6	9029.0
7	10677.0
8	11020.5
9	10315.5
10	8143.5
11	6088.5
12	4867.0
13	4538.0
14	4499.0
15	4569.5
16	4658.5
17	4662.0
18	4705.0
19	4820.0
20	5106.0
21	5335.0
22	5698.0
23	5922.0
24	6730.0
25	8108.5
26	8898.0
27	11421.0
28	15938.5
29	18291.0
30	19918.5
31	23127.0
32	23520.0
33	23622.5
34	25720.5
35	27303.5
36	28079.0
37	32416.0
38	40439.0
39	44394.0
40	51470.0
41	66007.0
42	80722.5
43	87708.0
44	87736.5
45	89802.0
46	93873.5
47	97169.0
48	90143.0
49	72734.0
50	63612.0
51	55136.5
52	42779.0
53	36654.5
54	32889.0
55	31046.5
56	30280.0
57	29833.0
58	27382.5
59	20335.0
60	13180.5
61	9825.5
62	8104.5
63	5882.5
64	4584.0
65	3789.0
66	2547.5
67	1749.0
68	1172.5
69	825.5
70	810.0
71	917.0
72	678.0
73	331.0
74	223.5
75	188.5
76	97.0
77	38.5
78	30.0
79	24.0
80	23.5
81	19.0
82	8.5
83	2.5
84	3.5
85	4.0
86	4.0
87	2.5
88	1.0
89	1.0
90	2.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1486047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.444550806032424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53734450583096	15.852161791143002
2	9.166916811036726	3.7482699295582615
3	3.308995356006906	2.0295277101842557
4	1.7627123430425846	1.441514482150183
5	1.114424425002141	1.139195338821987
6	0.7775030498296229	0.953742036245213
7	0.5717252966191655	0.818206681167714
8	0.4138616144375193	0.6768971842427571
9	0.34283307017381953	0.6308161309041058
>10	2.8039879409766084	12.803017425532474
>50	0.9016994746238371	13.37024252370586
>100	1.2897053861015222	43.60608973339099
>500	0.005306064843821972	0.7923116200622804
>1k	0.002653032421910986	0.8960457274296677
>5k	0.0	0.0
>10k+	3.316290527388732E-4	1.2419616854613085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18318	1.2326662615650785	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	2254	0.15167757143616586	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2213	0.1489185739078239	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG	2166	0.14575582064362702	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2051	0.13801716903974098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1594835156626944	0.0
2	0.0	0.0	0.0	0.5043582067054406	0.0
3	0.0	0.0	0.0	0.7331531236898967	0.0
4	0.0	0.0	0.0	1.1085786654123322	0.0
5	0.0	0.0	0.0	2.0140681956896382	0.0
6	0.0	0.0	0.0	2.7640444750401567	0.0
7	0.0	0.0	0.0	3.313623324161349	0.0
8	0.0	0.0	0.0	4.22247748557078	0.0
9	0.0	0.0	0.0	4.532965646443214	0.0
10	0.0	0.0	0.0	5.0221157204314535	0.0
11	0.0	0.0	0.0	5.846786810915132	0.0
12	0.0	0.0	0.0	6.41978349271591	0.0
13	0.0	0.0	0.0	6.617623803284822	0.0
14	0.0	0.0	0.0	6.684243499700885	0.0
15	0.0	0.0	0.0	6.789691039381662	0.0
16	0.0	0.0	0.0	7.033088455479537	0.0
17	0.0	0.0	0.0	7.330185384446118	0.0
18	0.0	0.0	0.0	7.678357413998346	0.0
19	0.0	0.0	0.0	7.869333877057724	0.0
20	0.0	0.0	0.0	8.058762609796325	0.0
21	0.0	0.0	0.0	8.305255486535756	0.0
22	0.0	0.0	0.0	8.55538216489788	0.0
23	0.0	0.0	0.0	8.840501007034097	0.0
24	0.0	0.0	0.0	9.043590142169124	0.0
25	0.0	0.0	0.0	9.215859256133891	0.0
26	0.0	0.0	0.0	9.374198797211664	0.0
27	0.0	0.0	0.0	9.523251956364772	0.0
28	0.0	0.0	0.0	9.688993685933218	0.0
29	0.0	0.0	0.0	9.865233064633891	0.0
30	0.0	0.0	0.0	10.081780724297415	0.0
31	0.0	0.0	0.0	10.29435811922503	0.0
32	0.0	0.0	0.0	10.48264287737871	0.0
33	6.729262264248708E-5	0.0	0.0	10.669178027343685	0.0
34	6.729262264248708E-5	0.0	0.0	10.86008719778042	0.0
35	6.729262264248708E-5	0.0	0.0	11.07650027219866	0.0
36	6.729262264248708E-5	0.0	0.0	11.274004119654359	0.0
37	6.729262264248708E-5	0.0	0.0	11.480727056412078	0.0
38	6.729262264248708E-5	0.0	0.0	11.680249682547053	0.0
39	6.729262264248708E-5	0.0	0.0	11.897470268437	0.0
40	6.729262264248708E-5	0.0	0.0	12.102241719138089	0.0
41	6.729262264248708E-5	0.0	0.0	12.319731475518607	0.0
42	1.3458524528497416E-4	0.0	0.0	12.533452845031146	0.0
43	1.3458524528497416E-4	0.0	0.0	12.736340102298245	0.0
44	1.3458524528497416E-4	0.0	0.0	12.941919064471042	0.0
45	1.3458524528497416E-4	0.0	0.0	13.146892393040059	0.0
46	1.3458524528497416E-4	0.0	0.0	13.384300765722752	0.0
47	1.3458524528497416E-4	0.0	0.0	13.620632456443168	0.0
48	1.3458524528497416E-4	0.0	0.0	13.859252096333426	0.0
49	1.3458524528497416E-4	0.0	0.0	14.10931148207291	0.0
50	1.3458524528497416E-4	0.0	0.0	14.370003102189903	0.0
51	1.3458524528497416E-4	0.0	0.0	14.606873133891458	0.0
52	1.3458524528497416E-4	0.0	0.0	14.861979466329126	0.0
53	1.3458524528497416E-4	0.0	0.0	15.08848643414374	0.0
54	1.3458524528497416E-4	0.0	0.0	15.33504660350581	0.0
55	1.3458524528497416E-4	0.0	0.0	15.58288533269809	0.0
56	1.3458524528497416E-4	0.0	0.0	15.823591043890268	0.0
57	1.3458524528497416E-4	0.0	0.0	16.074121477988246	0.0
58	1.3458524528497416E-4	0.0	0.0	16.30365661382177	0.0
59	1.3458524528497416E-4	0.0	0.0	16.56259862575006	0.0
60	2.0187786792746126E-4	0.0	0.0	16.825376317168974	0.0
61	2.0187786792746126E-4	0.0	0.0	17.109822233078766	0.0
62	2.0187786792746126E-4	0.0	0.0	17.399247803064103	0.0
63	2.0187786792746126E-4	0.0	0.0	17.760474601408973	0.0
64	3.364631132124354E-4	0.0	0.0	18.073116126205967	0.0
65	3.364631132124354E-4	0.0	0.0	18.325194290624726	0.0
66	4.037557358549225E-4	0.0	0.0	18.55883427643944	0.0
67	4.037557358549225E-4	0.0	0.0	18.80418317859395	0.0
68	4.7104835849740955E-4	0.0	0.0	19.093945211692496	0.0
69	4.7104835849740955E-4	0.0	0.0	19.39279174884778	0.0
70	5.383409811398966E-4	0.0	0.0	19.637467724775863	0.0
71	5.383409811398966E-4	0.0	0.0	19.896880785062653	0.0
72	5.383409811398966E-4	0.0	0.0	20.158514501896644	0.0
73	5.383409811398966E-4	0.0	0.0	20.4338759137497	0.0
74	5.383409811398966E-4	0.0	0.0	20.715899295244363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	4.0610757E-8	80.00001	1
GCGATAT	70	0.0	80.0	9
TAATGCG	25	1.2849905E-6	80.0	1
CGTTTTT	7505	0.0	74.45702	1
ACGATTG	85	0.0	70.58824	1
TATTACG	120	0.0	70.00001	1
GTCGACG	80	0.0	70.0	1
TATTGCG	150	0.0	69.333336	1
GTAGGGT	635	0.0	68.661415	4
GGCGATA	300	0.0	68.0	8
AGGGCGA	600	0.0	68.0	6
TTACGGG	515	0.0	67.572815	3
TAGGGTA	665	0.0	67.36842	5
TAGGGCG	275	0.0	66.90909	5
GTTACGG	90	0.0	66.66667	2
ACGGGAT	495	0.0	66.26262	5
TACGGGT	115	0.0	66.08696	4
GCGCGAC	55	9.094947E-12	65.454544	9
TAACGGG	410	0.0	65.36585	3
CGAGGGA	790	0.0	64.81013	4
>>END_MODULE