FastQCFastQC Report
Thu 26 May 2016
SRR1547260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547260_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1723874
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162670.9436304509494312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG700.074.285711
CGTTTTT100650.073.760551
TATAGCG1650.072.727271
CGAGGGA10950.067.9452064
TACGGGA7450.066.040274
GGGCGAT12350.062.510127
GTACGCG459.427822E-962.2222251
TAGCGGG11900.062.1848753
ATAGCGG4300.061.3953482
TTGCGGG10750.061.3953483
TCGATAG1250.060.81
CGTAAGG2450.060.408162
TGCGGGA11650.060.0858354
GCGAGGG14850.060.0673373
GTACGGG7550.059.867553
AGGGATT22950.059.259266
ACGGGAT6550.058.6259545
TAGGGAT21150.058.4397165
ATAGGGA19750.058.3291134
TAGGGAC17400.058.160925
Produced by FastQC (version 0.11.5)