##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547260_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1723874
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05637999064897	33.0	31.0	34.0	31.0	34.0
2	32.496291492301644	34.0	31.0	34.0	31.0	34.0
3	32.587669980520616	34.0	31.0	34.0	31.0	34.0
4	36.148780595333534	37.0	35.0	37.0	35.0	37.0
5	36.134729684420094	37.0	35.0	37.0	35.0	37.0
6	36.182406022713955	37.0	36.0	37.0	35.0	37.0
7	36.35887019584958	37.0	37.0	37.0	35.0	37.0
8	36.34660885888412	37.0	37.0	37.0	35.0	37.0
9	38.12841251738816	39.0	39.0	39.0	37.0	39.0
10-11	37.72946369630263	39.0	37.5	39.0	35.0	39.0
12-13	37.15392424272308	39.0	36.0	39.0	34.5	39.0
14-15	38.34693486878972	40.0	37.0	41.0	34.0	41.0
16-17	38.4728628658475	40.0	37.0	41.0	34.0	41.0
18-19	38.28093961623645	40.0	36.0	41.0	34.0	41.0
20-21	38.04233865120072	40.0	35.0	41.0	34.0	41.0
22-23	37.85545173255122	40.0	35.0	41.0	34.0	41.0
24-25	37.82391346467317	40.0	35.0	41.0	34.0	41.0
26-27	37.8126118846273	40.0	35.0	41.0	34.0	41.0
28-29	37.71634034738038	40.0	35.0	41.0	34.0	41.0
30-31	37.52281460245935	40.0	35.0	41.0	33.5	41.0
32-33	37.095076554318936	40.0	35.0	41.0	32.5	41.0
34-35	36.64082525753042	40.0	35.0	41.0	31.0	41.0
36-37	36.391842733285614	39.5	35.0	41.0	30.0	41.0
38-39	36.27939338954007	39.0	35.0	41.0	30.0	41.0
40-41	36.14165536460321	39.0	35.0	41.0	30.0	41.0
42-43	36.0625695381449	39.0	35.0	41.0	30.0	41.0
44-45	35.93562145493232	39.0	35.0	41.0	29.0	41.0
46-47	35.79887828228745	39.0	35.0	41.0	29.0	41.0
48-49	35.68177488609956	38.0	35.0	40.5	28.0	41.0
50-51	35.451288203198146	38.0	35.0	40.0	27.5	41.0
52-53	35.26578479633662	38.0	35.0	40.0	27.0	41.0
54-55	35.05907537325814	37.0	35.0	40.0	26.5	41.0
56-57	34.79793302758786	37.0	34.5	40.0	26.0	41.0
58-59	34.54720617632147	36.0	34.0	40.0	26.0	41.0
60-61	34.178472730605606	36.0	34.0	39.5	25.0	41.0
62-63	33.77923995605247	35.0	33.5	39.0	23.5	40.5
64-65	33.392536519490406	35.0	33.0	38.5	22.5	40.0
66-67	33.03162412102044	35.0	33.0	38.0	21.0	40.0
68-69	32.522760654200944	35.0	33.0	37.0	19.0	39.5
70-71	32.17626781307683	35.0	32.5	36.5	18.5	39.0
72-73	31.893156344373196	35.0	32.0	36.0	18.0	39.0
74-75	31.592298509055766	35.0	32.0	36.0	17.0	38.0
76-77	29.685291384405126	33.0	29.0	34.5	12.0	36.0
78-79	30.69894435440177	34.0	31.0	35.0	15.0	36.0
80-81	30.803302909609403	34.5	31.0	35.0	12.5	36.0
82-83	30.59220424462577	34.0	31.0	35.0	10.5	36.0
84-85	30.356701533870805	34.0	31.0	35.0	10.0	36.0
86	29.93289068690635	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	16.0
11	24.0
12	28.0
13	72.0
14	213.0
15	695.0
16	1850.0
17	4493.0
18	10977.0
19	19492.0
20	22761.0
21	20936.0
22	16977.0
23	13482.0
24	12061.0
25	12519.0
26	14337.0
27	17508.0
28	21335.0
29	27069.0
30	34513.0
31	44136.0
32	54510.0
33	70694.0
34	170454.0
35	161422.0
36	152000.0
37	271309.0
38	386353.0
39	161347.0
40	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.232271036050196	23.771690970453758	28.660215305759007	16.33582268773704
2	33.02283113499014	24.630918501004135	29.04643842879468	13.299811935211043
3	28.34569115840253	24.335073213007448	33.96379317745961	13.35544245113042
4	25.36426676195592	27.006614172497528	33.75948590210189	13.86963316344466
5	24.24910405284841	30.933293268533546	30.804397537174992	14.013205141443052
6	22.487374367268142	39.34353670859935	27.56703796217125	10.602050961961258
7	84.76164731297067	3.7677927737178005	9.332178569895481	2.13838134341605
8	84.95255453704853	3.5199208294805766	9.37406098125501	2.153463652215881
9	80.3393983551002	5.847875192734504	10.826487318678742	2.986239133486554
10-11	37.16338897158377	26.34310860306496	23.385931918458077	13.107570506893197
12-13	28.20716595296408	24.174243593209248	30.03026903358366	17.588321420243012
14-15	17.967496464358764	29.659998352547806	34.85071994820967	17.521785234883755
16-17	22.09743867591251	25.237227314757344	33.41894477206571	19.24638923726444
18-19	23.431874951417562	26.61018148658197	31.16689502829093	18.79104853370954
20-21	25.966746989629176	26.584976628222247	30.54941950513785	16.898856877010733
22-23	22.36509164822951	24.017532603891002	32.666598602914135	20.95077714496535
24-25	20.4821814123306	26.19243053726664	34.26984802833618	19.055540022066577
26-27	19.20453583034491	31.265307093209827	30.419131560659306	19.111025515785958
28-29	18.3837681872341	27.3747675294134	34.50458096125355	19.736883322098947
30-31	21.5445270361987	27.6072671204508	30.823221418734782	20.02498442461572
32-33	23.60915008869558	27.447771704892588	29.463609289310007	19.47946891710183
34-35	20.328834938052317	28.095933925565326	30.61000978029717	20.96522135608519
36-37	22.477077791068258	28.221929212923914	29.579888089268707	19.72110490673912
38-39	20.72407844192789	29.211908758992827	28.913134022556175	21.15087877652311
40-41	21.214601531202394	27.361976571373546	29.22841228535264	22.195009612071416
42-43	21.067433002644044	27.253180916934767	29.229079387472634	22.45030669294856
44-45	20.201505446453744	30.07687916866314	28.11684612680509	21.604769258078026
46-47	21.02047481428457	29.214200109752802	28.371969181042232	21.393355894920393
48-49	21.19815601372258	26.465768379823583	30.73754230297574	21.598533303478096
50-51	19.86865049301747	29.248657384472416	29.18673290507311	21.695959217437004
52-53	21.620402651237853	26.59710048414211	30.53752768473798	21.244969179882055
54-55	21.564076028758482	26.833544679019468	30.604179887857235	20.99819940436482
56-57	21.55998640271853	26.37315720290462	29.764704380946633	22.302152013430216
58-59	22.713638003705608	27.408267657613028	28.750216083077994	21.12787825560337
60-61	22.812195090824503	28.783223135797627	27.77415866820893	20.630423105168937
62-63	21.679310668877193	27.79916049548865	29.499719817109604	21.021809018524557
64-65	20.179810125333987	30.394245751139586	28.987936473315333	20.438007650211095
66-67	19.85330714425764	29.199175809832965	26.607658100301997	24.339858945607393
68-69	19.36086976194316	30.746040603895647	26.457647136623674	23.435442497537522
70-71	19.9729214548163	29.6876685883075	28.36399296004232	21.975416996833875
72-73	21.393471912680393	30.036330961543594	27.34176047669377	21.22843664908224
74-75	20.433279926491146	30.757497357695517	26.37878406426456	22.43043865154878
76-77	22.788266427824773	28.854893107036826	26.838098376099413	21.518742089038987
78-79	19.34984807474328	29.89360011230519	28.97415936431549	21.78239244863604
80-81	18.00789964927831	28.840593918116987	29.63119694362813	23.520309488976572
82-83	19.428015040542405	28.753348562597964	29.28917658715196	22.52945980970767
84-85	18.254756438115546	28.53642435584039	28.636953744879268	24.571865461164798
86	18.870346672668653	29.07764720623433	29.380975639750933	22.671030481346087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	445.0
1	577.0
2	4038.5
3	4906.0
4	2573.5
5	3054.0
6	3625.0
7	3845.0
8	4142.0
9	4438.0
10	4514.0
11	4565.0
12	4724.0
13	5045.0
14	5181.0
15	5374.5
16	5634.5
17	5807.5
18	6090.0
19	6582.0
20	6890.5
21	6883.0
22	7466.0
23	8770.0
24	9886.0
25	11052.5
26	12543.5
27	14067.5
28	16949.0
29	19026.0
30	21548.5
31	24950.5
32	26404.5
33	29549.5
34	34239.5
35	38134.5
36	39910.0
37	43896.0
38	49383.0
39	55369.0
40	63617.5
41	70692.0
42	77325.0
43	80647.0
44	83980.0
45	89671.0
46	93248.5
47	94293.5
48	90369.5
49	81944.5
50	77269.0
51	72301.0
52	63006.5
53	55575.5
54	51187.0
55	48034.5
56	42991.5
57	39817.0
58	36171.5
59	28094.0
60	21132.5
61	17348.0
62	14554.5
63	11021.5
64	9027.0
65	7977.0
66	5861.5
67	4403.5
68	3615.0
69	2736.5
70	1943.5
71	1633.0
72	1364.0
73	984.5
74	650.5
75	289.5
76	147.5
77	116.0
78	68.5
79	48.0
80	50.5
81	42.0
82	21.0
83	8.5
84	5.0
85	3.0
86	2.0
87	2.5
88	2.0
89	1.0
90	2.5
91	2.5
92	1.0
93	1.0
94	0.5
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1723874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.91739628562963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24900912587407	18.16180947381135
2	7.259819272715497	3.3275231046974505
3	2.5274165900591905	1.737654227197836
4	1.3585074049558745	1.2453381022534442
5	0.8911201784476636	1.0211077133803046
6	0.6073220663038456	0.8350944279895597
7	0.5009784005671473	0.803678437543675
8	0.42940481879871456	0.7872672319495299
9	0.32609138425462125	0.6725848930463413
>10	4.548035917242621	25.564708436164242
>50	1.7540767404314423	28.68368370849342
>100	0.5456741648554231	15.851989369286091
>500	0.002289541944542332	0.35918862786982814
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.543935493935924E-4	0.948372246316912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16267	0.9436304509494312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.8008879999350304E-5	0.0	0.0	0.05267206303941007	0.0
2	5.8008879999350304E-5	0.0	0.0	0.15540578951825945	0.0
3	5.8008879999350304E-5	0.0	0.0	0.24015676319731025	0.0
4	5.8008879999350304E-5	0.0	0.0	0.37937807519575095	0.0
5	5.8008879999350304E-5	0.0	0.0	0.705445989672099	0.0
6	5.8008879999350304E-5	0.0	0.0	1.0864483135078318	0.0
7	5.8008879999350304E-5	0.0	0.0	1.3376847727850179	0.0
8	5.8008879999350304E-5	0.0	0.0	1.7677046002202017	0.0
9	5.8008879999350304E-5	0.0	0.0	1.952056820858137	0.0
10	5.8008879999350304E-5	0.0	0.0	2.22945528501503	0.0
11	5.8008879999350304E-5	0.0	0.0	2.591372687330977	0.0
12	5.8008879999350304E-5	0.0	0.0	2.866334778527897	0.0
13	5.8008879999350304E-5	0.0	0.0	2.985020947006568	0.0
14	5.8008879999350304E-5	0.0	0.0	3.040535445165946	0.0
15	5.8008879999350304E-5	0.0	0.0	3.113568625085128	0.0
16	5.8008879999350304E-5	0.0	0.0	3.255284318923541	0.0
17	5.8008879999350304E-5	0.0	0.0	3.4330815361215494	0.0
18	5.8008879999350304E-5	0.0	0.0	3.65983824803901	0.0
19	5.8008879999350304E-5	0.0	0.0	3.8075288565173557	0.0
20	5.8008879999350304E-5	0.0	0.0	3.9690835873155463	0.0
21	5.8008879999350304E-5	0.0	0.0	4.176175288913226	0.0
22	5.8008879999350304E-5	0.0	0.0	4.410763199630599	0.0
23	5.8008879999350304E-5	0.0	0.0	4.6629278009877755	0.0
24	5.8008879999350304E-5	0.0	0.0	4.844843648665738	0.0
25	5.8008879999350304E-5	0.0	0.0	5.016839977863811	0.0
26	5.8008879999350304E-5	0.0	0.0	5.168707225702111	0.0
27	5.8008879999350304E-5	0.0	0.0	5.331190098580291	0.0
28	5.8008879999350304E-5	0.0	0.0	5.508117182578309	0.0
29	5.8008879999350304E-5	0.0	0.0	5.702388921696134	0.0
30	5.8008879999350304E-5	0.0	0.0	5.91429536033376	0.0
31	5.8008879999350304E-5	0.0	0.0	6.11245369441154	0.0
32	5.8008879999350304E-5	0.0	0.0	6.2987782169694535	0.0
33	5.8008879999350304E-5	0.0	0.0	6.485392783927364	0.0
34	5.8008879999350304E-5	0.0	0.0	6.672413413045269	0.0
35	5.8008879999350304E-5	0.0	0.0	6.888612508802847	0.0
36	5.8008879999350304E-5	0.0	0.0	7.081550043680687	0.0
37	5.8008879999350304E-5	0.0	0.0	7.29113612711834	0.0
38	5.8008879999350304E-5	0.0	0.0	7.500548183915994	0.0
39	5.8008879999350304E-5	0.0	0.0	7.7804990387928585	0.0
40	5.8008879999350304E-5	0.0	0.0	8.003253137990363	0.0
41	5.8008879999350304E-5	0.0	0.0	8.231924142947802	0.0
42	5.8008879999350304E-5	0.0	0.0	8.463321565265211	0.0
43	5.8008879999350304E-5	0.0	0.0	8.680854865262775	0.0
44	5.8008879999350304E-5	0.0	0.0	8.886728380380468	0.0
45	5.8008879999350304E-5	0.0	0.0	9.102985485018047	0.0
46	5.8008879999350304E-5	0.0	0.0	9.314717897015676	0.0
47	5.8008879999350304E-5	0.0	0.0	9.548609701173056	0.0
48	1.1601775999870061E-4	0.0	0.0	9.774264244370528	0.0
49	1.1601775999870061E-4	0.0	0.0	10.006705826527925	0.0
50	1.1601775999870061E-4	0.0	0.0	10.243846127965268	0.0
51	1.1601775999870061E-4	0.0	0.0	10.477969967642647	0.0
52	1.1601775999870061E-4	0.0	0.0	10.744172717959666	0.0
53	1.1601775999870061E-4	0.0	0.0	10.97493204259708	0.0
54	1.1601775999870061E-4	0.0	0.0	11.211782299634429	0.0
55	1.1601775999870061E-4	0.0	0.0	11.442251579871847	0.0
56	2.3203551999740121E-4	0.0	0.0	11.66773209642932	0.0
57	2.3203551999740121E-4	0.0	0.0	11.912413552266582	0.0
58	2.3203551999740121E-4	0.0	0.0	12.139518317464038	0.0
59	2.3203551999740121E-4	0.0	0.0	12.376484592261383	0.0
60	2.3203551999740121E-4	0.0	0.0	12.61704741761869	0.0
61	2.3203551999740121E-4	0.0	0.0	12.865383432895907	0.0
62	2.900443999967515E-4	0.0	0.0	13.144290127932784	0.0
63	2.900443999967515E-4	0.0	0.0	13.438801211689485	0.0
64	2.900443999967515E-4	0.0	0.0	13.697752852006586	0.0
65	3.480532799961018E-4	0.0	0.0	13.948583249123775	0.0
66	3.480532799961018E-4	0.0	0.0	14.177544298481212	0.0
67	3.480532799961018E-4	0.0	0.0	14.420137434638495	0.0
68	3.480532799961018E-4	0.0	0.0	14.697013818875394	0.0
69	3.480532799961018E-4	0.0	0.0	15.045183116631494	0.0
70	3.480532799961018E-4	0.0	0.0	15.309007502868539	0.0
71	3.480532799961018E-4	0.0	0.0	15.563550468305689	0.0
72	3.480532799961018E-4	0.0	0.0	15.81339471446289	0.0
73	4.060621599954521E-4	0.0	0.0	16.062832898460098	0.0
74	4.060621599954521E-4	0.0	0.0	16.333966403577058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACG	70	0.0	74.28571	1
CGTTTTT	10065	0.0	73.76055	1
TATAGCG	165	0.0	72.72727	1
CGAGGGA	1095	0.0	67.945206	4
TACGGGA	745	0.0	66.04027	4
GGGCGAT	1235	0.0	62.51012	7
GTACGCG	45	9.427822E-9	62.222225	1
TAGCGGG	1190	0.0	62.184875	3
ATAGCGG	430	0.0	61.395348	2
TTGCGGG	1075	0.0	61.395348	3
TCGATAG	125	0.0	60.8	1
CGTAAGG	245	0.0	60.40816	2
TGCGGGA	1165	0.0	60.085835	4
GCGAGGG	1485	0.0	60.067337	3
GTACGGG	755	0.0	59.86755	3
AGGGATT	2295	0.0	59.25926	6
ACGGGAT	655	0.0	58.625954	5
TAGGGAT	2115	0.0	58.439716	5
ATAGGGA	1975	0.0	58.329113	4
TAGGGAC	1740	0.0	58.16092	5
>>END_MODULE