FastQCFastQC Report
Thu 26 May 2016
SRR1547259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547259_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2208812
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297061.3448858481391808No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23000.10412837308018971No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC22360.10123088791621922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA150.001309951880.080
CGTTTTT134950.075.642831
TGTTACG1500.074.6666641
GCGTAAG1550.072.2580641
GTATCCG403.7161954E-970.000011
CTACGCG650.067.692311
CGCTAGG1900.067.368422
GCCGTAA1500.066.66666480
TACGGGA7550.066.225174
TAACGGG7250.065.6551743
CGAGGGA14050.064.911034
TTACGGG9550.062.8272253
TAAGGGA29050.062.3752174
GGGCGAT17450.061.8911137
CGAAGGG12100.061.4876023
TTAGCGG7050.061.2765922
ATAGGGA27550.060.9800384
TAGCGGG14950.060.7357863
GGCGATA3700.060.5405438
CGTAAGG4200.060.0000042
Produced by FastQC (version 0.11.5)