##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547259_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2208812
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.048926753385985	33.0	31.0	34.0	31.0	34.0
2	32.46408476592847	34.0	31.0	34.0	31.0	34.0
3	32.53599219852119	34.0	31.0	34.0	31.0	34.0
4	36.091290702875575	37.0	35.0	37.0	35.0	37.0
5	36.06032926297032	37.0	35.0	37.0	35.0	37.0
6	36.11200817453002	37.0	36.0	37.0	35.0	37.0
7	36.365454823678974	37.0	37.0	37.0	35.0	37.0
8	36.37071511744775	37.0	37.0	37.0	35.0	37.0
9	38.1847957182413	39.0	39.0	39.0	37.0	39.0
10-11	37.67471314896876	39.0	37.5	39.0	35.0	39.0
12-13	36.90295099809309	39.0	35.0	39.0	33.5	39.0
14-15	38.02388003143771	40.0	35.0	41.0	33.0	41.0
16-17	38.198986830929925	40.0	35.0	41.0	34.0	41.0
18-19	38.021279991235104	40.0	36.0	41.0	34.0	41.0
20-21	37.780749108570575	40.0	35.0	41.0	34.0	41.0
22-23	37.583630929205384	39.0	35.0	41.0	33.5	41.0
24-25	37.527893048389814	39.0	35.0	41.0	34.0	41.0
26-27	37.48702130375967	39.0	35.0	41.0	34.0	41.0
28-29	37.41598515401039	39.0	35.0	41.0	33.5	41.0
30-31	37.22045606416481	39.0	35.0	41.0	33.0	41.0
32-33	36.7984190143842	39.0	35.0	41.0	32.5	41.0
34-35	36.388451574873734	39.0	35.0	41.0	31.0	41.0
36-37	36.14570683245111	39.0	35.0	41.0	30.0	41.0
38-39	36.0306481493219	38.5	35.0	41.0	30.0	41.0
40-41	35.89631304067526	38.0	35.0	41.0	29.5	41.0
42-43	35.856288810455574	38.0	35.0	41.0	30.0	41.0
44-45	35.734392062339396	38.0	35.0	41.0	29.0	41.0
46-47	35.6123248606038	38.0	35.0	40.5	29.0	41.0
48-49	35.49725440644111	38.0	35.0	40.0	29.0	41.0
50-51	35.27486562912552	37.5	35.0	40.0	27.5	41.0
52-53	35.093188782024	37.0	35.0	40.0	27.0	41.0
54-55	34.90904431884651	37.0	35.0	40.0	27.0	41.0
56-57	34.632712743320845	36.0	34.0	40.0	26.0	41.0
58-59	34.3874777482194	36.0	34.0	40.0	25.5	41.0
60-61	34.01101383911352	35.0	34.0	39.0	25.0	41.0
62-63	33.60433821438855	35.0	33.5	39.0	23.5	40.0
64-65	33.18864212979647	35.0	33.0	38.0	22.0	40.0
66-67	32.81606266173853	35.0	33.0	37.5	20.0	40.0
68-69	32.30475273585982	35.0	32.5	37.0	18.5	39.5
70-71	31.952484412435282	35.0	32.0	36.5	17.5	39.0
72-73	31.636334826141834	35.0	32.0	36.0	15.0	38.5
74-75	31.322118858463284	35.0	32.0	35.5	13.5	37.5
76-77	29.407946896340658	33.0	29.0	34.5	11.0	35.5
78-79	30.367750401573335	34.0	30.5	35.0	11.0	36.0
80-81	30.49270195924325	34.5	31.0	35.0	10.0	36.0
82-83	30.27580459541147	34.5	31.0	35.0	10.0	36.0
84-85	30.03078917535761	34.0	31.0	35.0	10.0	35.5
86	29.629984806312173	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	9.0
11	11.0
12	28.0
13	85.0
14	307.0
15	958.0
16	2523.0
17	6697.0
18	15899.0
19	25780.0
20	26972.0
21	23792.0
22	19131.0
23	15914.0
24	15792.0
25	17479.0
26	20784.0
27	25427.0
28	31621.0
29	39620.0
30	51151.0
31	63215.0
32	77500.0
33	98835.0
34	249135.0
35	247984.0
36	177546.0
37	311329.0
38	450023.0
39	192971.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.51047893618832	24.50443949054967	30.51291825651074	15.472163316751267
2	31.845670885525795	24.606847481813755	30.958768786116696	12.588712846543753
3	26.9759943354165	23.893749219037204	35.99129305708227	13.138963388464026
4	24.48397600157913	26.469115524544417	35.01203361807161	14.03487485580484
5	23.67603037288823	30.65100153385621	32.81492494607961	12.858043147175948
6	21.627689454783837	40.311488709767964	28.232054153997716	9.828767681450481
7	86.51071254592966	3.2235880645342387	8.84991570129101	1.4157836882450838
8	87.2671825397544	2.596463619357374	8.538073860518686	1.598279980369538
9	83.34928459280373	4.885748538128189	9.645728110857782	2.119238758210296
10-11	40.605289178074	26.23731671142678	21.61818208158956	11.539212028909658
12-13	28.447848888904986	26.94688366415974	28.691418735501255	15.91384871143402
14-15	16.46274105718368	32.33670407440742	35.57871833365628	15.621836534752617
16-17	20.311031450390526	25.68650025443542	33.9863465066289	20.016121788545156
18-19	22.57254125747234	28.321129186186965	31.048432369979878	18.05789718636081
20-21	25.099057774043242	27.99120069974267	30.58123552389248	16.32850600232161
22-23	20.53146668888072	26.264254268810568	31.10606516081948	22.098213881489233
24-25	18.696249386548065	28.53142322660326	34.60955481951384	18.16277256733484
26-27	17.98487150558762	34.106048862465435	30.098238329020305	17.81084130292664
28-29	16.39204694650337	28.978405586351396	35.95339938392222	18.67614808322302
30-31	21.50909629248664	29.522883794546573	31.122046602427005	17.845973310539783
32-33	24.027124082991218	29.195309514798	29.7068741024587	17.070692299752082
34-35	18.840331363647064	30.322318060568303	30.18638526049297	20.650965315291657
36-37	22.336599946034337	30.07865766756066	30.02145497217509	17.56328741422991
38-39	19.909186476712367	31.41718263030081	28.559379431115005	20.114251461871813
40-41	20.075497597803707	28.77060157224789	28.95997939163677	22.193921438311637
42-43	21.543300199383197	27.74111151152746	28.86026515611107	21.85532313297827
44-45	19.031157925617933	32.2866545455204	27.72207412853606	20.960113400325607
46-47	20.888468552325865	30.795174057366587	28.098045465164077	20.21831192514347
48-49	21.38348578330795	27.799898769112087	30.60092936836634	20.215686079213622
50-51	19.097686901375038	31.45994317307222	27.912380048641534	21.52998987691121
52-53	21.58388762828163	27.97334042009913	30.140636686146216	20.302135265473023
54-55	21.509911210189006	28.456586617602586	30.470202986945015	19.563299185263393
56-57	21.953067984056588	26.691452237673463	29.618704534383188	21.736775243886758
58-59	22.611793126803008	28.6938861252112	28.56261646532163	20.131704282664163
60-61	23.340488008938742	30.023944998487874	26.898961975940004	19.736605016633376
62-63	21.58300480077073	28.26379067118433	30.32510236271806	19.828102165326882
64-65	19.10090129897882	32.04279042308716	29.725526663201755	19.130781614732264
66-67	18.225838142856883	31.12555527586775	26.41845480738062	24.230151773894747
68-69	17.734601224549667	33.24775942905055	25.836264018848144	23.181375327551642
70-71	19.091756111430037	31.04605552668131	28.19119508586516	21.67099327602349
72-73	21.40772958495336	31.180449037763285	27.167930090926706	20.243891286356646
74-75	20.385256871114425	32.5748184997184	25.452641510459017	21.587283118708157
76-77	24.157035546710176	30.008393652334377	26.35215219765195	19.482418603303493
78-79	18.915711251115987	31.22993265157922	29.042897267852585	20.811458829452214
80-81	17.065033149041202	30.252665233618796	30.580805428438456	22.101496188901546
82-83	18.5120327126075	28.914321363701394	30.701277428771668	21.872368494919442
84-85	16.884370421747075	28.75654424188206	30.122550040474245	24.23653529589662
86	18.256058007652985	28.90612691347204	30.67775799841725	22.16005708045773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	935.0
1	1299.5
2	9563.0
3	10468.0
4	4078.0
5	6412.5
6	9714.5
7	11286.0
8	11998.5
9	12132.0
10	10523.0
11	8857.5
12	8091.5
13	7901.5
14	7842.0
15	8100.0
16	8292.5
17	8304.5
18	8608.5
19	8992.5
20	9733.0
21	10316.0
22	9928.5
23	9880.5
24	10434.0
25	11678.0
26	12843.0
27	14713.0
28	18854.0
29	21260.0
30	22515.0
31	26420.5
32	32099.0
33	36934.5
34	41771.5
35	48908.5
36	53016.0
37	56991.5
38	64542.0
39	74284.0
40	87651.0
41	101312.5
42	114070.5
43	120367.0
44	124306.0
45	127159.5
46	124614.5
47	127217.0
48	128136.0
49	113502.5
50	102012.0
51	91116.5
52	73839.0
53	62703.0
54	55675.5
55	51857.0
56	46553.0
57	42794.0
58	37453.0
59	26995.5
60	18908.0
61	14769.5
62	11628.5
63	7842.5
64	6030.0
65	4924.5
66	3250.0
67	2315.0
68	1549.0
69	977.0
70	671.0
71	537.0
72	476.0
73	343.5
74	231.0
75	130.5
76	75.0
77	63.5
78	55.5
79	63.0
80	48.0
81	21.5
82	10.5
83	9.0
84	6.5
85	4.0
86	2.0
87	2.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2208812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.748658247426395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64501966040791	16.110299775485075
2	9.060523959271274	3.759874303470767
3	3.204932843975371	1.9949416885679199
4	1.7255074313303789	1.432078559842744
5	1.1106187430419643	1.1521924371281993
6	0.7599228977944994	0.9460428300439204
7	0.544396014288592	0.7906840796214537
8	0.447681476733314	0.7431031971554163
9	0.3487809235796822	0.6513062567918911
>10	2.9952557835169706	13.921908598963896
>50	0.9593378720832456	14.390132830784088
>100	1.193617090165882	41.904778398137246
>500	0.002863447477093171	0.4130096212692963
>1k	0.001321591143273771	0.432021683065028
>5k	0.0	0.0
>10k+	2.2026519054562852E-4	1.3576257396730902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29706	1.3448858481391808	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2300	0.10412837308018971	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	2236	0.10123088791621922	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07293513436181984	0.0
2	0.0	0.0	0.0	0.22034469207881885	0.0
3	0.0	0.0	0.0	0.33502172208408865	0.0
4	0.0	0.0	0.0	0.5372118586824048	0.0
5	0.0	0.0	0.0	1.0116297810768866	0.0
6	0.0	0.0	0.0	1.432308408320853	0.0
7	0.0	0.0	0.0	1.735186154367144	0.0
8	0.0	0.0	0.0	2.316539388594412	0.0
9	0.0	0.0	0.0	2.516058406057193	0.0
10	0.0	0.0	0.0	2.8442891472882255	0.0
11	0.0	0.0	0.0	3.3838099394606695	0.0
12	0.0	0.0	0.0	3.766640166750271	0.0
13	0.0	0.0	0.0	3.9026861498398233	0.0
14	0.0	0.0	0.0	3.956968723458583	0.0
15	0.0	0.0	0.0	4.034204812360672	0.0
16	0.0	0.0	0.0	4.213486706881346	0.0
17	0.0	0.0	0.0	4.453706336256775	0.0
18	0.0	0.0	0.0	4.745175234469932	0.0
19	0.0	0.0	0.0	4.9400763849526355	0.0
20	0.0	0.0	0.0	5.134388983761407	0.0
21	0.0	0.0	0.0	5.3871945643178325	0.0
22	0.0	0.0	0.0	5.65525721519079	0.0
23	0.0	0.0	0.0	5.9700870875384595	0.0
24	0.0	0.0	0.0	6.1787512925500225	0.0
25	0.0	0.0	0.0	6.3787683152753605	0.0
26	0.0	0.0	0.0	6.5542472605183235	0.0
27	0.0	0.0	0.0	6.743308167467399	0.0
28	4.527320568703901E-5	0.0	0.0	6.947943057172815	0.0
29	4.527320568703901E-5	0.0	0.0	7.182141350191868	0.0
30	4.527320568703901E-5	0.0	0.0	7.44585777331887	0.0
31	4.527320568703901E-5	0.0	0.0	7.685398304608993	0.0
32	4.527320568703901E-5	0.0	0.0	7.910994688547508	0.0
33	4.527320568703901E-5	0.0	0.0	8.131203561009267	0.0
34	9.054641137407801E-5	0.0	0.0	8.3672127822558	0.0
35	9.054641137407801E-5	0.0	0.0	8.626718797253908	0.0
36	9.054641137407801E-5	0.0	0.0	8.87069610270136	0.0
37	9.054641137407801E-5	0.0	0.0	9.132194138749698	0.0
38	9.054641137407801E-5	0.0	0.0	9.412525828363844	0.0
39	9.054641137407801E-5	0.0	0.0	9.799475917370966	0.0
40	9.054641137407801E-5	0.0	0.0	10.088907521328208	0.0
41	9.054641137407801E-5	0.0	0.0	10.376075465001096	0.0
42	9.054641137407801E-5	0.0	0.0	10.665325976135588	0.0
43	9.054641137407801E-5	0.0	0.0	10.935018462413279	0.0
44	9.054641137407801E-5	0.0	0.0	11.207110428592383	0.0
45	9.054641137407801E-5	0.0	0.0	11.483956081368627	0.0
46	9.054641137407801E-5	0.0	0.0	11.777960279100258	0.0
47	9.054641137407801E-5	0.0	0.0	12.08586335097781	0.0
48	9.054641137407801E-5	0.0	0.0	12.382765033873412	0.0
49	9.054641137407801E-5	0.0	0.0	12.691709389481767	0.0
50	9.054641137407801E-5	0.0	0.0	13.00893874173085	0.0
51	9.054641137407801E-5	0.0	0.0	13.320599489680426	0.0
52	9.054641137407801E-5	0.0	0.0	13.690481580143533	0.0
53	9.054641137407801E-5	0.0	0.0	13.991729490785092	0.0
54	9.054641137407801E-5	0.0	0.0	14.324215913350706	0.0
55	9.054641137407801E-5	0.0	0.0	14.635378656037725	0.0
56	9.054641137407801E-5	0.0	0.0	14.956410957564518	0.0
57	9.054641137407801E-5	0.0	0.0	15.277533805502687	0.0
58	9.054641137407801E-5	0.0	0.0	15.586840346756537	0.0
59	9.054641137407801E-5	0.0	0.0	15.91430144349089	0.0
60	9.054641137407801E-5	0.0	0.0	16.241943633047992	0.0
61	9.054641137407801E-5	0.0	0.0	16.579681747473302	0.0
62	1.3581961706111701E-4	0.0	0.0	16.945262883396143	0.0
63	1.3581961706111701E-4	0.0	0.0	17.324290161408033	0.0
64	1.3581961706111701E-4	0.0	0.0	17.689418565274003	0.0
65	1.3581961706111701E-4	0.0	0.0	18.023036817981794	0.0
66	1.3581961706111701E-4	0.0	0.0	18.33881742764889	0.0
67	1.3581961706111701E-4	0.0	0.0	18.66890437031309	0.0
68	1.3581961706111701E-4	0.0	0.0	19.028781082319366	0.0
69	1.3581961706111701E-4	0.0	0.0	19.515739682689155	0.0
70	1.3581961706111701E-4	0.0	0.0	19.86533937700447	0.0
71	1.3581961706111701E-4	0.0	0.0	20.222182784229712	0.0
72	1.3581961706111701E-4	0.0	0.0	20.587084822067247	0.0
73	1.8109282274815603E-4	0.0	0.0	20.942434213504818	0.0
74	1.8109282274815603E-4	0.0	0.0	21.319831656111973	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	15	0.0013099518	80.0	80
CGTTTTT	13495	0.0	75.64283	1
TGTTACG	150	0.0	74.666664	1
GCGTAAG	155	0.0	72.258064	1
GTATCCG	40	3.7161954E-9	70.00001	1
CTACGCG	65	0.0	67.69231	1
CGCTAGG	190	0.0	67.36842	2
GCCGTAA	150	0.0	66.666664	80
TACGGGA	755	0.0	66.22517	4
TAACGGG	725	0.0	65.655174	3
CGAGGGA	1405	0.0	64.91103	4
TTACGGG	955	0.0	62.827225	3
TAAGGGA	2905	0.0	62.375217	4
GGGCGAT	1745	0.0	61.891113	7
CGAAGGG	1210	0.0	61.487602	3
TTAGCGG	705	0.0	61.276592	2
ATAGGGA	2755	0.0	60.980038	4
TAGCGGG	1495	0.0	60.735786	3
GGCGATA	370	0.0	60.540543	8
CGTAAGG	420	0.0	60.000004	2
>>END_MODULE