FastQCFastQC Report
Thu 26 May 2016
SRR1547258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547258_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2134992
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108890.5100253303056873No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28610.13400518596790995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG503.6379788E-1272.01
CGTTTTT73000.071.397261
GGGCGAT15250.069.5081947
CGTTAGG2600.069.2307742
CGAAACG3800.068.4210580
TACGGGA9650.065.906734
TTACGGG6500.064.03
GGCGATT6000.064.08
CGAGGGA13450.061.8587344
CGATAGG4800.061.6666642
GGCGATA3050.061.6393478
ACGTTAG1450.060.689661
GCGATAG2250.060.4444431
AGGGATT34100.059.9413536
AGGGCGA8950.059.888276
TAGGGAG24800.059.354845
TAAGGGA26400.059.0909044
TACGGGG11800.058.9830554
TTGCGGG12100.058.842983
GTAGGGC6500.058.461544
Produced by FastQC (version 0.11.5)