##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547258_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2134992
Sequences flagged as poor quality	0
Sequence length	86
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.074719717919315	33.0	31.0	34.0	31.0	34.0
2	32.49419810472358	34.0	31.0	34.0	31.0	34.0
3	32.61365897389779	34.0	31.0	34.0	31.0	34.0
4	36.12970072019005	37.0	35.0	37.0	35.0	37.0
5	36.110621491790134	37.0	35.0	37.0	35.0	37.0
6	36.16948072873341	37.0	36.0	37.0	35.0	37.0
7	36.34856524052549	37.0	37.0	37.0	35.0	37.0
8	36.335135213621406	37.0	37.0	37.0	35.0	37.0
9	38.09976383986451	39.0	39.0	39.0	37.0	39.0
10-11	37.701103329661194	39.0	37.5	39.0	35.0	39.0
12-13	36.79443037725669	39.0	35.0	39.0	33.5	39.0
14-15	37.82980381191123	40.0	35.0	41.0	33.0	41.0
16-17	38.01256538666187	40.0	35.0	41.0	34.0	41.0
18-19	37.890765867038375	40.0	36.0	41.0	34.0	41.0
20-21	37.70191129521797	40.0	35.0	41.0	34.0	41.0
22-23	37.51630380816415	39.0	35.0	41.0	33.0	41.0
24-25	37.47865308160405	39.0	35.0	41.0	34.0	41.0
26-27	37.445185040506004	39.0	35.0	41.0	33.5	41.0
28-29	37.37104565263008	39.0	35.0	41.0	33.0	41.0
30-31	37.195243354541844	39.0	35.0	41.0	33.0	41.0
32-33	36.86032055389435	39.0	35.0	41.0	32.5	41.0
34-35	36.506501663706466	39.0	35.0	41.0	31.0	41.0
36-37	36.31821782001994	39.0	35.0	41.0	31.0	41.0
38-39	36.20753754580814	38.5	35.0	41.0	31.0	41.0
40-41	36.07359863643517	38.0	35.0	41.0	30.0	41.0
42-43	36.02202865397153	38.0	35.0	41.0	30.0	41.0
44-45	35.8862426650779	38.0	35.0	41.0	30.0	41.0
46-47	35.78218841101044	38.0	35.0	40.5	30.0	41.0
48-49	35.694088549277936	37.5	35.0	40.0	30.0	41.0
50-51	35.46219517450183	37.0	35.0	40.0	29.0	41.0
52-53	35.27921088228902	37.0	35.0	40.0	29.0	41.0
54-55	35.084833104761046	36.0	34.5	40.0	28.5	41.0
56-57	34.80698803555236	36.0	34.0	40.0	27.5	41.0
58-59	34.58223824726275	36.0	34.0	39.5	27.0	41.0
60-61	34.2558405371074	35.0	34.0	39.0	26.0	41.0
62-63	33.879853413970636	35.0	33.5	39.0	26.0	40.0
64-65	33.608109070197926	35.0	33.0	38.0	25.5	40.0
66-67	33.34710762382248	35.0	33.0	37.0	25.0	40.0
68-69	32.95638531666629	35.0	33.0	37.0	24.0	39.5
70-71	32.704559314507975	35.0	33.0	36.0	24.0	39.0
72-73	32.50015245958767	35.0	33.0	36.0	24.0	38.5
74-75	32.237928994581715	35.0	33.0	35.5	23.0	37.5
76-77	30.312262294191264	33.0	30.0	34.5	19.5	35.5
78-79	31.364626893215526	34.0	31.5	35.0	20.0	36.0
80-81	31.600375083372676	35.0	32.0	35.0	20.0	36.0
82-83	31.470116984044907	35.0	32.0	35.0	20.0	36.0
84-85	31.27048157557499	34.0	32.0	35.0	19.5	36.0
86	30.84720130098848	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	17.0
11	18.0
12	37.0
13	111.0
14	327.0
15	947.0
16	2395.0
17	5118.0
18	10430.0
19	17084.0
20	20807.0
21	20432.0
22	17839.0
23	15113.0
24	14863.0
25	16094.0
26	18314.0
27	21851.0
28	26496.0
29	33097.0
30	41436.0
31	51836.0
32	66932.0
33	93979.0
34	272478.0
35	241358.0
36	179532.0
37	320842.0
38	438060.0
39	186676.0
40	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.55113274429131	23.562055501847315	28.549146788372042	14.337664965489333
2	31.812859251931624	24.490255701192325	29.632851083282745	14.064033963593307
3	29.66273409923784	24.75030351401785	32.01843379272616	13.56852859401815
4	26.317194631174264	27.670689164174856	31.229718893560253	14.782397311090628
5	24.750162998268845	30.785876481035995	29.609291276032884	14.854669244662274
6	23.601634104483765	39.43996043076508	26.34234695024618	10.616058514504973
7	85.59507482932021	4.297439990407458	7.7642445498624815	2.343240630409856
8	86.12819158104573	3.4801067170275113	7.974221917459176	2.4174797844675764
9	81.86840981137166	5.717726342768498	9.004061841917908	3.409802003941935
10-11	44.308924810959475	25.104871587340842	19.3504472147905	11.235756386909177
12-13	31.63550495739563	25.70670054032989	26.257264664223566	16.400529838050915
14-15	17.769621619191078	29.978472987252413	35.84861207910849	16.403293314448018
16-17	22.529616036031985	23.298424537422154	33.208695863965765	20.963263562580096
18-19	25.76063516865637	25.475060328094905	29.99643558383357	18.767868919415154
20-21	27.819097214415795	25.713164264784133	28.983176517757443	17.484562003042633
22-23	22.816338421876992	24.14322863973261	30.78732847710905	22.253104461281353
24-25	21.17525498924586	25.698995593426112	33.743779836177374	19.381969581150653
26-27	19.003677765537294	31.964218132901667	29.334442470978818	19.69766163058222
28-29	18.071730479552148	27.524248334419987	34.59183922000644	19.81218196602142
30-31	23.231749814519212	26.855627562070488	30.81575949699109	19.09686312641921
32-33	27.33160124253393	26.97984348419104	27.796169728036453	17.892385545238575
34-35	21.18780772949032	28.072212916957067	29.739736729692662	21.00024262385995
36-37	23.799597375540518	29.28687788994057	27.97375821548746	18.939766519031455
38-39	21.999801404408075	30.228356827566564	27.045768789765955	20.726072978259403
40-41	21.659706453232612	26.88394148549503	27.67216457953941	23.784187481732953
42-43	22.14500101171339	25.55264844083725	27.735092215802215	24.567258331647142
44-45	20.50923375825296	30.371659472260315	26.896400548573485	22.222706220913242
46-47	21.449869601384925	29.56474778359825	26.84122469779746	22.144157917219363
48-49	22.218584425609087	25.691548258728837	30.538311150580423	21.55155616508165
50-51	20.037264776636164	30.57662511147583	27.538159393571497	21.847950718316508
52-53	22.88594055621754	25.701548296199704	29.931447049918685	21.481064097664067
54-55	22.64980384001439	26.37958362373255	30.156951407780454	20.813661128472614
56-57	23.377698839152558	24.698101913262438	28.884909170619842	23.03929007696516
58-59	24.264353215375046	27.594318854590554	27.19626115695047	20.94506677308393
60-61	25.675904172006263	27.92663860098773	26.57869444007284	19.81876278693316
62-63	23.390204740814017	25.21459096802236	29.868214962866375	21.52698932829725
64-65	20.24588382532581	29.25275598222382	29.76425672789406	20.73710346455631
66-67	20.805417537864308	28.240269752767222	25.527660056805836	25.42665265256263
68-69	19.850917474163836	30.62027867083343	25.295996425279345	24.23280742972339
70-71	20.54885919947241	28.648327487878174	28.344321664905536	22.458491647743877
72-73	22.65476872981257	29.989433215674815	26.068270981811644	21.287527072700975
74-75	21.984321252726005	30.339247172823132	25.324872411699907	22.351559162750963
76-77	25.37583279000577	27.161694282695205	25.718105735290813	21.744367192008212
78-79	20.286773908286307	28.65584508044995	29.25736958264949	21.80001142861425
80-81	18.18880351776494	27.820338436865338	29.296245606540914	24.694612438828813
82-83	19.632111033671322	27.67024419763634	30.113204171256847	22.584440597435492
84-85	18.561615219167095	26.736634141954628	28.442963720707148	26.258786918171122
86	20.02653874112877	27.23944633047805	29.60891656736887	23.125098361024303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	571.0
1	997.5
2	3635.0
3	4010.0
4	2341.0
5	2833.5
6	3328.5
7	3498.0
8	3690.5
9	4019.0
10	4154.5
11	4151.0
12	4273.5
13	4478.0
14	4557.0
15	4619.0
16	4822.0
17	4946.5
18	4984.5
19	5867.0
20	6513.5
21	6332.0
22	6323.0
23	7173.0
24	9168.5
25	12895.5
26	15589.5
27	16394.0
28	17589.0
29	18083.0
30	21002.0
31	24801.5
32	30677.5
33	36896.5
34	39430.0
35	43118.5
36	45497.0
37	47937.5
38	50774.0
39	56956.5
40	68622.5
41	84850.5
42	99410.0
43	103621.0
44	104318.5
45	109594.5
46	116744.5
47	122482.0
48	120605.0
49	104331.0
50	93100.0
51	86559.5
52	75717.0
53	71782.5
54	70030.0
55	66363.5
56	64306.0
57	63795.0
58	61720.0
59	49190.5
60	34042.5
61	27266.0
62	22854.0
63	17149.5
64	13774.0
65	11469.0
66	8286.5
67	6754.5
68	5203.0
69	3831.5
70	3017.0
71	2677.0
72	2395.5
73	1926.0
74	1242.5
75	781.0
76	606.0
77	332.5
78	207.5
79	147.0
80	139.5
81	87.0
82	30.5
83	19.0
84	14.5
85	12.0
86	14.0
87	9.0
88	4.5
89	4.0
90	1.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2134992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.11549250996039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.08298218875096	13.066921921480676
2	8.189909244134443	2.639688421622079
3	2.362765867946647	1.1423140694305276
4	1.1653723491383687	0.7512219745541728
5	0.6404588479912366	0.5160654883870317
6	0.42336012421603536	0.40935941464916503
7	0.29757921085598893	0.33569448805687285
8	0.23117016471723503	0.2980336846421528
9	0.2019767415508671	0.29294591990842833
>10	2.299637573803231	8.927594135180266
>50	1.1757049971696227	13.916442578664215
>100	1.922626000363692	56.43403812558435
>500	0.005869717601471749	0.6193502213006539
>1k	2.934858800735874E-4	0.13531584445522496
>5k	0.0	0.0
>10k+	2.934858800735874E-4	0.5150137120842169
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10889	0.5100253303056873	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2861	0.13400518596790995	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02824366555003485	0.0
2	0.0	0.0	0.0	0.08571460689314059	0.0
3	0.0	0.0	0.0	0.1329747371418722	0.0
4	0.0	0.0	0.0	0.23096105278146242	0.0
5	0.0	0.0	0.0	0.42627794389861884	0.0
6	0.0	0.0	0.0	0.5847797087764264	0.0
7	0.0	0.0	0.0	0.6895576189512654	0.0
8	0.0	0.0	0.0	0.8962562857378388	0.0
9	0.0	0.0	0.0	0.9587389554621282	0.0
10	0.0	0.0	0.0	1.0844068736557326	0.0
11	0.0	0.0	0.0	1.3015505444516888	0.0
12	0.0	0.0	0.0	1.4828158606683304	0.0
13	0.0	0.0	0.0	1.5484367154537346	0.0
14	0.0	0.0	0.0	1.5739169046066683	0.0
15	0.0	0.0	0.0	1.6200997474463605	0.0
16	0.0	0.0	0.0	1.7066106102505303	0.0
17	0.0	0.0	0.0	1.8112011660933625	0.0
18	0.0	0.0	0.0	1.9476887969603633	0.0
19	0.0	0.0	0.0	2.041974864542818	0.0
20	0.0	0.0	0.0	2.1412258219234546	0.0
21	0.0	0.0	0.0	2.2644581338009697	0.0
22	0.0	0.0	0.0	2.4038029182310754	0.0
23	0.0	0.0	0.0	2.5364497852919357	0.0
24	0.0	0.0	0.0	2.642445498624819	0.0
25	0.0	0.0	0.0	2.7300336488380283	0.0
26	0.0	0.0	0.0	2.8216030786063837	0.0
27	0.0	0.0	0.0	2.9270835675262483	0.0
28	0.0	0.0	0.0	3.0305968359600413	0.0
29	0.0	0.0	0.0	3.1680212384870763	0.0
30	0.0	0.0	0.0	3.3091927276542488	0.0
31	0.0	0.0	0.0	3.4529871774695176	0.0
32	0.0	0.0	0.0	3.5808096704812007	0.0
33	0.0	0.0	0.0	3.7062433957597967	0.0
34	0.0	0.0	0.0	3.83706355808359	0.0
35	0.0	0.0	0.0	3.98362148429596	0.0
36	0.0	0.0	0.0	4.125308197876151	0.0
37	0.0	0.0	0.0	4.268212714614387	0.0
38	0.0	0.0	0.0	4.445871459939896	0.0
39	0.0	0.0	0.0	4.732429910744396	0.0
40	0.0	0.0	0.0	4.910276010401913	0.0
41	0.0	0.0	0.0	5.0868574683183825	0.0
42	0.0	0.0	0.0	5.252103989148437	0.0
43	0.0	0.0	0.0	5.3977251437007725	0.0
44	0.0	0.0	0.0	5.550091054205355	0.0
45	0.0	0.0	0.0	5.695524854425684	0.0
46	0.0	0.0	0.0	5.8695770288600615	0.0
47	0.0	0.0	0.0	6.063957148317184	0.0
48	0.0	0.0	0.0	6.231358243965317	0.0
49	4.6838583001716166E-5	0.0	0.0	6.405035709735681	0.0
50	4.6838583001716166E-5	0.0	0.0	6.586207348786319	0.0
51	4.6838583001716166E-5	0.0	0.0	6.773280649295173	0.0
52	9.367716600343233E-5	0.0	0.0	6.998199524869414	0.0
53	9.367716600343233E-5	0.0	0.0	7.19023771517645	0.0
54	9.367716600343233E-5	0.0	0.0	7.373657606211171	0.0
55	9.367716600343233E-5	0.0	0.0	7.563681737449134	0.0
56	9.367716600343233E-5	0.0	0.0	7.76283939237243	0.0
57	9.367716600343233E-5	0.0	0.0	7.958858862234613	0.0
58	9.367716600343233E-5	0.0	0.0	8.142138237520328	0.0
59	9.367716600343233E-5	0.0	0.0	8.333754880580349	0.0
60	9.367716600343233E-5	0.0	0.0	8.544809535586081	0.0
61	9.367716600343233E-5	0.0	0.0	8.768978993832295	0.0
62	9.367716600343233E-5	0.0	0.0	9.028324227912798	0.0
63	9.367716600343233E-5	0.0	0.0	9.311744493656182	0.0
64	9.367716600343233E-5	0.0	0.0	9.546827341741796	0.0
65	1.405157490051485E-4	0.0	0.0	9.761722760553669	0.0
66	1.405157490051485E-4	0.0	0.0	9.955400301265765	0.0
67	1.405157490051485E-4	0.0	0.0	10.16593973185848	0.0
68	1.405157490051485E-4	0.0	0.0	10.426690123429035	0.0
69	1.405157490051485E-4	0.0	0.0	10.8214925395505	0.0
70	1.405157490051485E-4	0.0	0.0	11.07451456492577	0.0
71	1.405157490051485E-4	0.0	0.0	11.310299991756409	0.0
72	1.405157490051485E-4	0.0	0.0	11.542478847695916	0.0
73	1.405157490051485E-4	0.0	0.0	11.765711534282095	0.0
74	1.8735433200686466E-4	0.0	0.0	12.027679729010694	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	50	3.6379788E-12	72.0	1
CGTTTTT	7300	0.0	71.39726	1
GGGCGAT	1525	0.0	69.508194	7
CGTTAGG	260	0.0	69.230774	2
CGAAACG	380	0.0	68.42105	80
TACGGGA	965	0.0	65.90673	4
TTACGGG	650	0.0	64.0	3
GGCGATT	600	0.0	64.0	8
CGAGGGA	1345	0.0	61.858734	4
CGATAGG	480	0.0	61.666664	2
GGCGATA	305	0.0	61.639347	8
ACGTTAG	145	0.0	60.68966	1
GCGATAG	225	0.0	60.444443	1
AGGGATT	3410	0.0	59.941353	6
AGGGCGA	895	0.0	59.88827	6
TAGGGAG	2480	0.0	59.35484	5
TAAGGGA	2640	0.0	59.090904	4
TACGGGG	1180	0.0	58.983055	4
TTGCGGG	1210	0.0	58.84298	3
GTAGGGC	650	0.0	58.46154	4
>>END_MODULE