FastQCFastQC Report
Thu 26 May 2016
SRR1547257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547257_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1294721
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.5586531770165155No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG18190.1404935889662715No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC17420.1345463617258081No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG15630.12072098930966595No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT14350.11083468948136317No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT14090.10882653482873916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.856852E-444.01
TAATACG454.802132E-1044.01
TTGCGTA207.856852E-444.016
CGTTTTT72150.042.84131
CGCGACC4950.040.010
GCGTAAG501.3478711E-939.61
TACGGGT1950.039.487184
TAGCCGT452.3514076E-839.1111144
GCGCGAC5050.038.7722749
TAACGCG404.125086E-738.51
AGCGCAG800.038.51
TAGTACG404.125086E-738.51
TAGGGTA8350.038.4670685
TAGGGCG6950.038.302165
AATACGG1150.038.2608682
AGGGCGC4150.038.1686746
CACGACG1450.037.93103426
TACGGGA3200.037.81254
AGGGCGA15950.037.7931026
ACAACGA4250.037.78823513