##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547257_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1294721
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39995180428834	31.0	31.0	33.0	30.0	34.0
2	31.82841863227676	31.0	31.0	34.0	30.0	34.0
3	31.95571941754247	33.0	31.0	34.0	30.0	34.0
4	35.62820329630863	37.0	35.0	37.0	33.0	37.0
5	35.49064084076801	37.0	35.0	37.0	33.0	37.0
6	35.55454186654885	37.0	35.0	37.0	33.0	37.0
7	35.99317922548565	37.0	35.0	37.0	35.0	37.0
8	35.99241072014743	37.0	35.0	37.0	35.0	37.0
9	37.66905534088039	39.0	37.0	39.0	35.0	39.0
10	37.08938605305699	39.0	37.0	39.0	33.0	39.0
11	36.876415845576	39.0	37.0	39.0	32.0	39.0
12	36.50566260993681	39.0	35.0	39.0	32.0	39.0
13	36.44507735643432	39.0	35.0	39.0	32.0	39.0
14	37.50042596049651	40.0	36.0	41.0	32.0	41.0
15	37.61707889190026	40.0	36.0	41.0	33.0	41.0
16	37.666906615402084	40.0	35.0	41.0	33.0	41.0
17	37.55750852886452	39.0	35.0	41.0	32.0	41.0
18	37.461305563129045	39.0	36.0	41.0	32.0	41.0
19	37.40416352248863	39.0	36.0	41.0	32.0	41.0
20	37.22493263027324	39.0	35.0	41.0	32.0	41.0
21	37.096382154919866	39.0	35.0	41.0	32.0	41.0
22	37.05806501941345	39.0	35.0	41.0	32.0	41.0
23	36.962270635913065	39.0	35.0	41.0	32.0	41.0
24	36.89297771488992	38.0	35.0	41.0	32.0	41.0
25	36.72300132615444	38.0	35.0	41.0	31.0	41.0
26	36.646448926062064	38.0	35.0	41.0	31.0	41.0
27	36.45959554220562	38.0	35.0	40.0	31.0	41.0
28	36.419220048180264	38.0	35.0	40.0	31.0	41.0
29	36.36620090351512	38.0	35.0	40.0	30.0	41.0
30	36.322328130925506	38.0	35.0	40.0	30.0	41.0
31	36.20055981172778	38.0	35.0	40.0	30.0	41.0
32	36.00872852143435	38.0	35.0	40.0	30.0	41.0
33	35.7992053886513	38.0	35.0	41.0	29.0	41.0
34	35.68245513898361	38.0	35.0	41.0	28.0	41.0
35	35.43677595404724	38.0	35.0	41.0	26.0	41.0
36	35.319372281750276	38.0	35.0	40.0	25.0	41.0
37	35.293966808293064	38.0	35.0	40.0	25.0	41.0
38	35.21237007818673	38.0	34.0	40.0	25.0	41.0
39	35.15274951128467	38.0	34.0	40.0	25.0	41.0
40	35.08646573277177	38.0	34.0	40.0	24.0	41.0
41	35.07987975787834	38.0	34.0	40.0	24.0	41.0
42	34.90038780555811	38.0	34.0	40.0	24.0	41.0
43	34.872230387859624	38.0	34.0	40.0	24.0	41.0
44	34.637223772534774	37.0	34.0	40.0	23.0	41.0
45	34.47028046969193	37.0	34.0	40.0	23.0	41.0
46	34.49495064959941	37.0	34.0	40.0	23.0	41.0
47	34.35998257539655	37.0	33.0	40.0	23.0	41.0
48	34.30854060450089	37.0	33.0	40.0	23.0	41.0
49	34.22082209217275	37.0	33.0	40.0	23.0	41.0
50	33.65015397139615	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	4.0
13	4.0
14	7.0
15	24.0
16	63.0
17	204.0
18	501.0
19	1008.0
20	1788.0
21	2964.0
22	4512.0
23	6766.0
24	10171.0
25	15503.0
26	22065.0
27	25875.0
28	26634.0
29	27652.0
30	31750.0
31	37661.0
32	45590.0
33	57914.0
34	89374.0
35	142682.0
36	97122.0
37	123140.0
38	186375.0
39	337364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.47087287531445	25.40964423995594	28.01831437043193	15.101168514297674
2	32.83549119848987	26.53459702901243	26.941789003190646	13.688122769307054
3	28.240756116568743	25.10749420145344	32.81031202861466	13.841437653363156
4	24.16088099289345	28.070680864834973	32.33391595563832	15.43452218663326
5	22.19343009034379	31.75749833361782	31.329529682456688	14.719541893581706
6	19.18714533864825	40.177922502222486	28.973732564776505	11.66119959435276
7	84.51450157987705	3.484380032454869	9.743180190944614	2.257938196723464
8	83.84377792590064	2.8785352211016892	9.01924043867366	4.258446414324013
9	77.73759752101031	4.842973891672414	12.967890379471717	4.451538207845552
10	37.99544457840724	23.642236435494596	21.703285881668716	16.65903310442945
11	32.10985223843593	25.69101760147553	25.410030423542988	16.789099736545555
12	27.181995194331442	21.68814748505663	30.519625463709943	20.61023185690199
13	21.589361723491006	29.88497135676335	32.22068692791729	16.304979991828354
14	18.581918421034338	32.45602720586134	30.888585262770896	18.073469110333424
15	17.115424867596957	25.34414750359344	40.15436530341286	17.386062325396743
16	18.247020014350582	22.379493342581146	39.63131825312172	19.74216838994656
17	17.885474940160854	24.787734191381773	31.04344488117517	26.283345987282203
18	21.661578054268062	23.492319967004473	33.3683473118919	21.47775466683556
19	23.855332538824968	28.94546392620495	27.264252298371616	19.934951236598465
20	25.904190941523307	24.570776252180973	27.50507638325168	22.019956423044036
21	22.508324187218715	29.58969538611021	28.318533490999222	19.583446935671855
22	21.6995785192331	24.59711397281731	27.818966402800292	25.884341105149293
23	19.52969018035546	29.878792419370658	26.833194178514137	23.758323221759746
24	20.36871264156525	25.49159239712648	33.813539751035165	20.3261552102731
25	20.580881904286716	26.1569094808843	30.847649802544332	22.414558812284653
26	19.304081728804892	30.001984983637403	29.47553951778028	21.21839376977743
27	21.590365800817317	27.47340932911415	28.68741605334277	22.248808816725766
28	20.370489086065646	27.35739977956641	32.64919623610029	19.622914898267656
29	20.191531611829884	24.54698734321912	31.5627845690307	23.698696475920293
30	22.80529936565484	25.942423116640573	30.346537979997233	20.90573953770735
31	24.16412493502461	25.189674068776206	28.23728046428536	22.408920531913825
32	25.363842866532636	24.33945228354217	28.878731402363904	21.417973447561288
33	23.203918064200703	24.783872355511342	28.54738588468095	23.464823695607006
34	21.04407049858618	25.42323790221986	31.053176707568657	22.4795148916253
35	21.39356664486017	24.20544657883822	30.42253890992731	23.9784478663743
36	24.59317490022947	23.7597134826731	29.38285545688994	22.264256160207488
37	22.304728200129603	26.270679165627186	30.08462827126462	21.339964362978588
38	21.284971820183653	26.731473421686992	28.700932478889275	23.282622279240083
39	21.918930796673568	25.72994490704947	28.57851228179662	23.77261201448034
40	23.757937038172702	23.731135897231916	29.054831118055553	23.456095946539833
41	19.42480271811456	23.67266770215359	29.957805581279672	26.944723998452176
42	22.8147994818961	25.420611853827968	27.603475961230256	24.161112703045674
43	21.92789025589297	24.70879826619017	29.605992333483428	23.757319144433435
44	22.41000184595755	26.737806832514494	28.76604303166474	22.08614828986322
45	20.564121536609044	26.835434043318983	27.767912932593198	24.83253148747877
46	23.794624478941795	26.129181499334607	27.393701036748457	22.68249298497514
47	22.961549244972467	24.921894369520537	28.364412101139933	23.75214428436706
48	22.591353658432975	23.338387189209104	29.520954707616543	24.549304444741377
49	22.663492752492623	22.282175078646286	29.82704381870689	25.227288350154204
50	20.868125256329357	24.484193891965912	30.422075489622863	24.225605362081872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	910.0
1	903.5
2	897.0
3	6551.0
4	12205.0
5	9053.5
6	5902.0
7	6392.0
8	6882.0
9	7329.5
10	7777.0
11	8179.0
12	8581.0
13	8453.0
14	8325.0
15	8137.5
16	7950.0
17	7748.5
18	7547.0
19	7250.5
20	6954.0
21	7026.5
22	7099.0
23	6961.5
24	6824.0
25	7545.5
26	8267.0
27	9242.0
28	10217.0
29	11791.5
30	13366.0
31	15956.5
32	18547.0
33	21079.0
34	23611.0
35	26728.5
36	29846.0
37	33563.0
38	37280.0
39	42429.5
40	47579.0
41	59788.5
42	71998.0
43	84827.0
44	97656.0
45	108512.5
46	119369.0
47	132378.5
48	145388.0
49	144174.0
50	142960.0
51	125108.5
52	107257.0
53	93841.5
54	80426.0
55	69818.5
56	59211.0
57	54257.5
58	49304.0
59	44571.5
60	39839.0
61	35348.0
62	30857.0
63	27337.0
64	23817.0
65	20164.0
66	16511.0
67	13670.5
68	10830.0
69	9373.5
70	7917.0
71	6976.5
72	6036.0
73	4932.5
74	3829.0
75	3151.5
76	2474.0
77	1920.5
78	1367.0
79	1023.0
80	679.0
81	461.5
82	244.0
83	173.5
84	103.0
85	82.0
86	61.0
87	37.0
88	13.0
89	8.5
90	4.0
91	2.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1294721.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.570149608246005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23166460385022	25.591083856368833
2	7.659418807226592	5.142556705416198
3	3.1039041051106744	3.1259557553464283
4	1.8385866259323047	2.4688651240107085
5	1.314471598910859	2.2063504115613943
6	1.0681821821656357	2.1515421398497847
7	0.8436590003703006	1.9825231202541986
8	0.7230724913221509	1.9418921369033413
9	0.6007119694028397	1.8149391615885684
>10	6.348439102826298	44.491296211743325
>50	0.22421196645792374	4.800037098189538
>100	0.03859338777546139	2.0660326343240705
>500	0.0025420793243292553	0.5915473837794868
>1k	0.002310981203935686	1.0642422309097703
>5k	2.3109812039356865E-4	0.5611360297543643
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7233	0.5586531770165155	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1819	0.1404935889662715	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1742	0.1345463617258081	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1563	0.12072098930966595	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	1435	0.11083468948136317	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	1409	0.10882653482873916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1425017436188955	0.0
2	0.0	0.0	0.0	0.552319766189009	0.0
3	0.0	0.0	0.0	0.9242145605114924	0.0
4	0.0	0.0	0.0	1.4498104224771207	0.0
5	0.0	0.0	0.0	2.378195765728678	0.0
6	0.0	0.0	0.0	4.100188380353759	0.0
7	0.0	0.0	0.0	4.9235317879296	0.0
8	0.0	0.0	0.0	7.155672921038587	0.0
9	0.0	0.0	0.0	8.278231371855403	0.0
10	0.0	0.0	0.0	9.870929721538463	0.0
11	0.0	0.0	0.0	10.908450546488394	0.0
12	0.0	0.0	0.0	11.781920583662426	0.0
13	0.0	0.0	0.0	12.28094701483949	0.0
14	0.0	0.0	0.0	12.49968139854069	0.0
15	0.0	0.0	0.0	12.728302082070192	0.0
16	0.0	0.0	0.0	13.174344125104945	0.0
17	0.0	0.0	0.0	13.636296931925875	0.0
18	0.0	0.0	0.0	14.289101667463493	0.0
19	0.0	0.0	0.0	14.577503570267263	0.0
20	0.0	0.0	0.0	14.920357358844106	0.0
21	0.0	0.0	0.0	15.274410471445199	0.0
22	0.0	0.0	0.0	15.648776840724759	0.0
23	0.0	0.0	0.0	16.085087057366028	0.0
24	0.0	0.0	0.0	16.428635976399548	0.0
25	0.0	0.0	0.0	16.711013415245446	0.0
26	0.0	0.0	0.0	16.98319560739341	0.0
27	0.0	0.0	0.0	17.283260254525878	0.0
28	0.0	0.0	0.0	17.592593307747382	0.0
29	0.0	0.0	0.0	17.88833269870497	0.0
30	0.0	0.0	0.0	18.270113792855756	0.0
31	7.723671740861544E-5	0.0	0.0	18.59080064353633	0.0
32	7.723671740861544E-5	0.0	0.0	18.905308556824213	0.0
33	7.723671740861544E-5	0.0	0.0	19.20784477891376	0.0
34	1.5447343481723088E-4	0.0	0.0	19.491921425542646	0.0
35	1.5447343481723088E-4	0.0	0.0	19.82419378383451	0.0
36	1.5447343481723088E-4	0.0	0.0	20.12927881759854	0.0
37	1.5447343481723088E-4	0.0	0.0	20.45236000651878	0.0
38	1.5447343481723088E-4	0.0	0.0	20.769416731481144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.856852E-4	44.0	1
TAATACG	45	4.802132E-10	44.0	1
TTGCGTA	20	7.856852E-4	44.0	16
CGTTTTT	7215	0.0	42.8413	1
CGCGACC	495	0.0	40.0	10
GCGTAAG	50	1.3478711E-9	39.6	1
TACGGGT	195	0.0	39.48718	4
TAGCCGT	45	2.3514076E-8	39.11111	44
GCGCGAC	505	0.0	38.772274	9
TAACGCG	40	4.125086E-7	38.5	1
AGCGCAG	80	0.0	38.5	1
TAGTACG	40	4.125086E-7	38.5	1
TAGGGTA	835	0.0	38.467068	5
TAGGGCG	695	0.0	38.30216	5
AATACGG	115	0.0	38.260868	2
AGGGCGC	415	0.0	38.168674	6
CACGACG	145	0.0	37.931034	26
TACGGGA	320	0.0	37.8125	4
AGGGCGA	1595	0.0	37.793102	6
ACAACGA	425	0.0	37.788235	13
>>END_MODULE