FastQCFastQC Report
Thu 26 May 2016
SRR1547256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547256_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences213944
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27901.3040795722245073No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT7330.3426130202295928No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC7020.32812324720487607TruSeq Adapter, Index 16 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT6780.31690535841154693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.29026287252739036No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG6160.28792581236211345TruSeq Adapter, Index 13 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT5450.25473955801518156No Hit
GAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC5380.25146767378379387No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC5000.23370601652768946No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG4120.19257375761881615TruSeq Adapter, Index 13 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT3640.17013798003215794No Hit
AAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC3620.16920315596604718No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT3110.14536514228022285No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC2780.12994054518939535No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT2600.12152712859439853No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT2430.11358112403245708No Hit
TCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG2230.1042328833713495TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.8421005E-444.00000425
ATTAGCG207.8421005E-444.0000041
GGTGTAA207.8421005E-444.0000048
ATCTAGG207.8421005E-444.0000042
CGACGGT207.8421005E-444.00000428
TTGCTAG207.8421005E-444.0000041
TCTTACA207.8421005E-444.00000432
CACGACG207.8421005E-444.00000426
AATGCGG207.8421005E-444.0000042
AACATAG207.8421005E-444.0000041
TGATTCG207.8421005E-444.00000415
CGGGCTC207.8421005E-444.0000046
GCGGGAT207.8421005E-444.0000045
CGGGAAC207.8421005E-444.0000046
CAAGCGA207.8421005E-444.00000415
CGTAAGG408.258212E-944.0000042
CGGTCTA207.8421005E-444.00000431
CCGAAGG207.8421005E-444.0000042
ACGACGG207.8421005E-444.00000427
CAACGAG254.43066E-544.014
Produced by FastQC (version 0.11.5)