##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547256_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	213944
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198444452753993	31.0	31.0	33.0	30.0	34.0
2	31.613492315746175	31.0	31.0	34.0	30.0	34.0
3	31.676686422615266	31.0	31.0	34.0	30.0	34.0
4	35.395271659873615	37.0	35.0	37.0	33.0	37.0
5	35.28275156115619	37.0	35.0	37.0	33.0	37.0
6	35.32453819691134	37.0	35.0	37.0	32.0	37.0
7	35.802186553490635	37.0	35.0	37.0	35.0	37.0
8	35.773090154432936	37.0	35.0	37.0	35.0	37.0
9	37.39885203604682	39.0	37.0	39.0	35.0	39.0
10	36.990347941517406	39.0	37.0	39.0	33.0	39.0
11	36.80697752682945	39.0	37.0	39.0	32.0	39.0
12	36.60719160154059	39.0	35.0	39.0	32.0	39.0
13	36.598465018883445	39.0	35.0	39.0	32.0	39.0
14	37.68930187338743	40.0	36.0	41.0	32.0	41.0
15	37.76253599072655	40.0	36.0	41.0	32.0	41.0
16	37.755249037131215	40.0	36.0	41.0	32.0	41.0
17	37.616329506786826	39.0	36.0	41.0	32.0	41.0
18	37.37302752122051	39.0	36.0	41.0	32.0	41.0
19	37.18264592603672	38.0	36.0	41.0	32.0	41.0
20	36.88373593089781	38.0	35.0	40.0	32.0	41.0
21	36.75850689900161	38.0	35.0	40.0	31.0	41.0
22	36.72720898926822	38.0	35.0	40.0	31.0	41.0
23	36.60693452492241	38.0	35.0	40.0	31.0	41.0
24	36.47976573308903	38.0	35.0	40.0	31.0	41.0
25	36.30030288299742	38.0	35.0	40.0	31.0	41.0
26	36.23162135886026	38.0	35.0	40.0	31.0	41.0
27	36.04400684291216	38.0	35.0	40.0	30.0	41.0
28	35.94126032980593	38.0	35.0	40.0	30.0	41.0
29	35.7784700669334	38.0	35.0	40.0	30.0	41.0
30	35.622602176270426	37.0	35.0	40.0	29.0	41.0
31	35.331624163332464	37.0	34.0	40.0	27.0	41.0
32	34.693480536962944	37.0	33.0	40.0	24.0	41.0
33	34.25548274314774	37.0	33.0	40.0	21.0	41.0
34	33.75250532849718	37.0	33.0	40.0	18.0	41.0
35	33.34961017836443	37.0	32.0	40.0	15.0	41.0
36	33.08878024155854	37.0	32.0	40.0	14.0	41.0
37	33.0357056052051	37.0	32.0	40.0	12.0	41.0
38	32.916618367423254	37.0	32.0	40.0	10.0	41.0
39	32.865399356841046	37.0	32.0	40.0	10.0	41.0
40	32.7711270238941	37.0	31.0	40.0	10.0	41.0
41	32.7159770781139	37.0	31.0	40.0	10.0	41.0
42	32.51806547507759	37.0	31.0	40.0	10.0	41.0
43	32.42630781886849	37.0	31.0	40.0	10.0	41.0
44	32.12347156265191	36.0	31.0	40.0	10.0	41.0
45	31.930173316381858	36.0	30.0	40.0	9.0	41.0
46	31.928607486071122	36.0	30.0	40.0	10.0	41.0
47	31.788617582171035	35.0	30.0	40.0	10.0	41.0
48	31.702038851288187	35.0	30.0	40.0	9.0	41.0
49	31.614572037542533	35.0	29.0	40.0	8.0	41.0
50	31.08661612384549	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	10.0
16	30.0
17	83.0
18	207.0
19	340.0
20	631.0
21	1022.0
22	1393.0
23	2065.0
24	3213.0
25	4721.0
26	6481.0
27	7210.0
28	6563.0
29	6306.0
30	6661.0
31	7131.0
32	8222.0
33	9624.0
34	13551.0
35	19140.0
36	16856.0
37	20149.0
38	28316.0
39	44014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.711513293198223	24.89576711662865	24.491923120068805	20.90079647010433
2	37.279848932430916	27.254328235426094	23.32105597726508	12.144766854877911
3	27.281438133343304	25.118722656396063	35.40552667987885	12.194312530381781
4	21.696331750364582	29.455838911116928	34.832946939386005	14.014882399132484
5	21.040552667987885	29.94194742549452	34.45247354447893	14.565026362038664
6	17.325561829263734	38.227760535467226	32.32574879407695	12.120928841192088
7	71.88890550798341	5.695883034812848	18.973189245783946	3.442022211419811
8	68.62730434132295	5.840313353026961	17.36435702800733	8.168025277642748
9	61.69605130314475	7.647795684852111	24.10584078076506	6.550312231238081
10	29.805930523875407	23.115862094753766	27.48522977975545	19.592977601615377
11	23.054163706390458	24.280185469094718	35.034868937665934	17.630781886848894
12	20.11367460643907	19.76171334554837	38.37546273791273	21.74914931009984
13	17.91543581497962	23.931028680402346	42.02127659574468	16.13225890887335
14	14.480892196088696	28.623845492278356	38.066035972030065	18.829226339602886
15	12.61217888793329	26.22321729050593	44.91923120068803	16.245372620872754
16	13.700314100886214	23.312175148637028	43.68199154919044	19.305519201286316
17	13.38995251093744	28.091930598661328	36.15058146056912	22.367535429832106
18	15.112833264779567	26.07224320382904	38.25954455371499	20.5553789776764
19	17.35220431514789	33.038084732453356	32.13177280035897	17.477938152039783
20	18.02761470291291	26.791122910668214	32.60198930561268	22.579273080806193
21	16.928729013199717	33.760703735556966	31.688198780989417	17.6223684702539
22	15.219403208316196	27.137007815129195	32.37716411771305	25.266424858841567
23	14.517817746700072	31.777474479303	30.476666791309874	23.22804098268706
24	15.884530531353999	27.768014059753952	36.4525296339229	19.894925774969153
25	18.751635942115694	27.55020005235015	32.172437647234794	21.52572635829937
26	15.551733163818568	29.59839958119882	34.04675989978686	20.803107355195753
27	19.65748046217702	25.995587630407957	31.724189507534682	22.62274239988034
28	20.001963130538833	26.49805556594249	35.134895112739784	18.365086190778896
29	20.122088023034067	25.423475301948173	31.4998317316681	22.95460494334966
30	24.32131772800359	25.949313839135474	30.909022921886102	18.820345510974835
31	24.674213812960403	24.661593688067907	28.60935571925364	22.054836779718055
32	24.655517331638187	25.425344950080397	30.897805033092773	19.021332685188646
33	23.48465018883446	24.99018434730584	29.827431477395955	21.697733986463746
34	20.561922746139178	27.01314362636952	31.016060277455782	21.408873350035524
35	20.137980032157948	24.511087013424074	30.903413977489436	24.447518976928542
36	21.38690498448192	29.798919343379577	28.72106719515387	20.093108476984632
37	18.460905657555248	26.987903376584526	29.295983995811987	25.25520697004824
38	17.5354298321056	31.927981153946828	28.68086976031111	21.855719253636465
39	18.057996485061512	26.534046292487755	26.37559361328198	29.032363609168755
40	20.408611599297014	26.462064839397225	30.130781886848894	22.998541674456867
41	16.794114347679766	24.016097670418425	30.461709606252104	28.7280783756497
42	21.493942340051603	27.259469767789703	27.945630632314998	23.300957259843695
43	20.12956661556295	23.515499383016117	32.345847511498334	24.009086489922595
44	22.390438619451817	25.444508843435663	29.54745166959578	22.61760086751673
45	19.405077964327113	24.240455446285008	31.18619825748794	25.168268331899935
46	23.529054331974724	24.82892719590173	28.39247653591594	23.249541936207603
47	21.87441573495868	23.30189208390981	29.830235949594286	24.993456231537227
48	19.776203118573086	23.26029241296788	30.90107691732416	26.062427551134874
49	20.8507833825674	21.176102157573943	30.228003589724413	27.745110870134244
50	18.284223909060316	21.031671839359834	35.247074000673074	25.43703025090678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	758.0
1	651.0
2	544.0
3	2523.5
4	4503.0
5	3297.0
6	2091.0
7	2344.0
8	2597.0
9	2805.5
10	3014.0
11	3173.0
12	3332.0
13	3368.5
14	3405.0
15	3343.5
16	3282.0
17	3191.5
18	3101.0
19	2932.0
20	2763.0
21	2632.5
22	2502.0
23	2409.0
24	2316.0
25	2219.5
26	2123.0
27	2138.5
28	2154.0
29	2156.0
30	2158.0
31	2271.5
32	2385.0
33	2563.5
34	2742.0
35	3224.5
36	3707.0
37	3975.5
38	4244.0
39	4957.0
40	5670.0
41	7027.0
42	8384.0
43	10454.0
44	12524.0
45	15749.5
46	18975.0
47	23427.0
48	27879.0
49	27720.5
50	27562.0
51	22139.0
52	16716.0
53	13352.0
54	9988.0
55	8539.5
56	7091.0
57	6562.5
58	6034.0
59	5528.5
60	5023.0
61	4468.5
62	3914.0
63	3695.5
64	3477.0
65	2880.0
66	2283.0
67	1904.5
68	1526.0
69	1337.5
70	1149.0
71	1026.5
72	904.0
73	714.0
74	524.0
75	410.5
76	297.0
77	213.0
78	129.0
79	105.5
80	82.0
81	73.0
82	64.0
83	39.5
84	15.0
85	11.5
86	8.0
87	5.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	213944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.02445119176167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0120199255911	42.02507249116732
2	12.26692698514062	13.744957043038294
3	5.433010352702711	9.131442699879866
4	2.841602094925722	6.367967914942952
5	1.5436650437595572	4.324149345026797
6	0.9127256136403866	3.0680970955719933
7	0.5489261407753633	2.1527300047226006
8	0.3139041929788442	1.4069048110746074
9	0.22900086596382646	1.1546683054255393
>10	0.82320281638742	7.89007699100936
>50	0.04046914070410594	1.5140882214960805
>100	0.026650409731972205	3.2255940818066606
>500	0.006909365486066869	2.451407262926269
>1k	9.870522122952669E-4	1.542843731911638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2790	1.3040795722245073	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	733	0.3426130202295928	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	702	0.32812324720487607	TruSeq Adapter, Index 16 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	678	0.31690535841154693	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.29026287252739036	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	616	0.28792581236211345	TruSeq Adapter, Index 13 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	545	0.25473955801518156	No Hit
GAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC	538	0.25146767378379387	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	500	0.23370601652768946	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	412	0.19257375761881615	TruSeq Adapter, Index 13 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	364	0.17013798003215794	No Hit
AAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC	362	0.16920315596604718	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	311	0.14536514228022285	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC	278	0.12994054518939535	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	260	0.12152712859439853	No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	243	0.11358112403245708	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	223	0.1042328833713495	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35476573308903264	0.0
2	0.0	0.0	0.0	1.090472273118199	0.0
3	0.0	0.0	0.0	1.5480686534794152	0.0
4	0.0	0.0	0.0	2.6469543431926112	0.0
5	0.0	0.0	0.0	4.479209512769697	0.0
6	0.0	0.0	0.0	8.343304790038514	0.0
7	0.0	0.0	0.0	10.120405339715065	0.0
8	0.0	0.0	0.0	14.530437871592566	0.0
9	0.0	0.0	0.0	17.02548330404218	0.0
10	0.0	0.0	0.0	20.449743858205885	0.0
11	0.0	0.0	0.0	22.457745952211795	0.0
12	4.6741203305537897E-4	0.0	0.0	24.21801966869835	0.0
13	4.6741203305537897E-4	0.0	0.0	25.20332423437909	0.0
14	4.6741203305537897E-4	0.0	0.0	25.662790262872527	0.0
15	4.6741203305537897E-4	0.0	0.0	26.037654713382942	0.0
16	4.6741203305537897E-4	0.0	0.0	26.656040833115206	0.0
17	4.6741203305537897E-4	0.0	0.0	27.314624387690237	0.0
18	4.6741203305537897E-4	0.0	0.0	28.349474628874844	0.0
19	4.6741203305537897E-4	0.0	0.0	28.72714355158359	0.0
20	4.6741203305537897E-4	0.0	0.0	29.259993269266722	0.0
21	4.6741203305537897E-4	0.0	0.0	29.719926709793217	0.0
22	4.6741203305537897E-4	0.0	0.0	30.16864226152638	0.0
23	4.6741203305537897E-4	0.0	0.0	30.67671914145758	0.0
24	4.6741203305537897E-4	0.0	0.0	31.077291253786036	0.0
25	4.6741203305537897E-4	0.0	0.0	31.422708746213964	0.0
26	4.6741203305537897E-4	0.0	0.0	31.74475563698912	0.0
27	4.6741203305537897E-4	0.0	0.0	32.11868526343342	0.0
28	4.6741203305537897E-4	0.0	0.0	32.444471450473024	0.0
29	4.6741203305537897E-4	0.0	0.0	32.77633399394234	0.0
30	4.6741203305537897E-4	0.0	0.0	33.16662304154358	0.0
31	4.6741203305537897E-4	0.0	0.0	33.51718206633512	0.0
32	4.6741203305537897E-4	0.0	0.0	33.83315260068055	0.0
33	9.348240661107579E-4	0.0	0.0	34.167819616348204	0.0
34	9.348240661107579E-4	0.0	0.0	34.51323710877613	0.0
35	9.348240661107579E-4	0.0	0.0	34.89651497588154	0.0
36	9.348240661107579E-4	0.0	0.0	35.23071457951613	0.0
37	9.348240661107579E-4	0.0	0.0	35.559305238754064	0.0
38	9.348240661107579E-4	0.0	0.0	35.879482481397	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.8421005E-4	44.000004	25
ATTAGCG	20	7.8421005E-4	44.000004	1
GGTGTAA	20	7.8421005E-4	44.000004	8
ATCTAGG	20	7.8421005E-4	44.000004	2
CGACGGT	20	7.8421005E-4	44.000004	28
TTGCTAG	20	7.8421005E-4	44.000004	1
TCTTACA	20	7.8421005E-4	44.000004	32
CACGACG	20	7.8421005E-4	44.000004	26
AATGCGG	20	7.8421005E-4	44.000004	2
AACATAG	20	7.8421005E-4	44.000004	1
TGATTCG	20	7.8421005E-4	44.000004	15
CGGGCTC	20	7.8421005E-4	44.000004	6
GCGGGAT	20	7.8421005E-4	44.000004	5
CGGGAAC	20	7.8421005E-4	44.000004	6
CAAGCGA	20	7.8421005E-4	44.000004	15
CGTAAGG	40	8.258212E-9	44.000004	2
CGGTCTA	20	7.8421005E-4	44.000004	31
CCGAAGG	20	7.8421005E-4	44.000004	2
ACGACGG	20	7.8421005E-4	44.000004	27
CAACGAG	25	4.43066E-5	44.0	14
>>END_MODULE