FastQCFastQC Report
Thu 26 May 2016
SRR1547255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547255_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1190132
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68700.577246893621884No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC29470.24761959177637438No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG23410.19670087015557935No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG20490.17216577656932172No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT17430.14645434287961337No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT16450.13821996215545837No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15870.1333465531554483No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC15870.1333465531554483No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT13500.11343279568988986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13030.10948365391401964No Hit
AAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC12820.10771914375884356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG551.8189894E-1244.0000041
TATAACG551.8189894E-1244.0000041
ACGCATT207.856596E-444.015
CCGGATA207.856596E-444.020
TATCTCG207.856596E-444.01
CTCGCTA207.856596E-444.012
GCGTATG351.4461511E-744.01
TCACGAC1300.043.99999625
CGTTTTT55450.042.6510351
CAACCCG800.041.2523
CGAATAT750.041.06666614
ATAACGG1450.040.9655152
CGGTCTA1350.040.7407431
ATAGCGG1900.040.5263142
CGTTTAT1150.040.17391639
TCGTTTA1100.040.00000438
CGCATCG1100.040.00000421
TACGGGA2650.039.8490564
GCAATAG1000.039.61
TAGGGAC9350.039.2941175